Acanthamoeba polyphaga Intracellular Infection of
... which coincided with the presence of host cell organelles dispersed between the bacteria, as well as the lysosomal enzyme acid phosphatase. We show that the type II secretion system, ...
... which coincided with the presence of host cell organelles dispersed between the bacteria, as well as the lysosomal enzyme acid phosphatase. We show that the type II secretion system, ...
Lipid Microdomains in Synapse Formation
... presynaptic vesicles and active zone markers around the SSBLMs (ratios of synaptophysin are 15.26 ± 2.34 (SS-BLMs) vs 2.24 ± 0.13 (uncoated beads), bassoon 11.65 ± 1.76 (SSBLMs) vs 1.26 ± 0.22 (uncoated beads), and actin 20.37 ± 2.74 (SS-BLMs) vs 1.76 ± 0.19 (uncoated beads); see Supporting Informat ...
... presynaptic vesicles and active zone markers around the SSBLMs (ratios of synaptophysin are 15.26 ± 2.34 (SS-BLMs) vs 2.24 ± 0.13 (uncoated beads), bassoon 11.65 ± 1.76 (SSBLMs) vs 1.26 ± 0.22 (uncoated beads), and actin 20.37 ± 2.74 (SS-BLMs) vs 1.76 ± 0.19 (uncoated beads); see Supporting Informat ...
PDF - The Journal of Immunology
... monocytic cells abrogates GAIT element–mediated translational silencing but, interestingly, overall protein synthesis was not inhibited (11). In addition to our studies, reports from other laboratories provided compelling evidence that controlling the protein synthesis of many inflammatory molecules ...
... monocytic cells abrogates GAIT element–mediated translational silencing but, interestingly, overall protein synthesis was not inhibited (11). In addition to our studies, reports from other laboratories provided compelling evidence that controlling the protein synthesis of many inflammatory molecules ...
Inflammation Protein L13a in Macrophages Resolves An
... monocytic cells abrogates GAIT element–mediated translational silencing but, interestingly, overall protein synthesis was not inhibited (11). In addition to our studies, reports from other laboratories provided compelling evidence that controlling the protein synthesis of many inflammatory molecules ...
... monocytic cells abrogates GAIT element–mediated translational silencing but, interestingly, overall protein synthesis was not inhibited (11). In addition to our studies, reports from other laboratories provided compelling evidence that controlling the protein synthesis of many inflammatory molecules ...
Nitrogen gas flushing can be bactericidal: the temperature
... bacteria in raw and pasteurized milk, respectively. In previous studies, nitrogen (N2 ) gas flushing treatments of raw and pasteurized milk at cold chain-temperatures inhibited bacterial spoilage and highlighted different susceptibilities to the N2 treatment with the exclusion of certain bacterial t ...
... bacteria in raw and pasteurized milk, respectively. In previous studies, nitrogen (N2 ) gas flushing treatments of raw and pasteurized milk at cold chain-temperatures inhibited bacterial spoilage and highlighted different susceptibilities to the N2 treatment with the exclusion of certain bacterial t ...
Localization of Enzymes in Bacillus megaterium
... It appears that repeated washing of the ‘ghost ’ fraction decreased its RNA content to some extent. It can also be concluded that at least 95% of the bacterial RNA was present in the soluble protoplasm of Bacillus megaterium strain M, since no nucleic acids have been found in the cell wall of this o ...
... It appears that repeated washing of the ‘ghost ’ fraction decreased its RNA content to some extent. It can also be concluded that at least 95% of the bacterial RNA was present in the soluble protoplasm of Bacillus megaterium strain M, since no nucleic acids have been found in the cell wall of this o ...
Inflammation Protein L13a in Macrophages Resolves An
... monocytic cells abrogates GAIT element–mediated translational silencing but, interestingly, overall protein synthesis was not inhibited (11). In addition to our studies, reports from other laboratories provided compelling evidence that controlling the protein synthesis of many inflammatory molecules ...
... monocytic cells abrogates GAIT element–mediated translational silencing but, interestingly, overall protein synthesis was not inhibited (11). In addition to our studies, reports from other laboratories provided compelling evidence that controlling the protein synthesis of many inflammatory molecules ...
Antigens and Antibodies - Thieme Medical Publishers
... The IgG molecule comprises a single Y-shaped unit with a molecular structure as described above. IgG consists of paired γ heavy chains with either paired κ or λ light chains. The molecule has two N-terminal antigen-binding sites and is therefore said to have an antigen-binding valence of two. IgG is ...
... The IgG molecule comprises a single Y-shaped unit with a molecular structure as described above. IgG consists of paired γ heavy chains with either paired κ or λ light chains. The molecule has two N-terminal antigen-binding sites and is therefore said to have an antigen-binding valence of two. IgG is ...
Quorum sensing, communication and cross
... (AQs), long-chain fatty acids and fatty acid methyl esters as well as autoinducer-2 (AI-2), a collective term for a group of interconvertible furanones derived from dihydroxypentanedione (DPD) (Fig. 1). AI-2 is also produced by some Gram-positive bacteria, although generally these organisms prefer l ...
... (AQs), long-chain fatty acids and fatty acid methyl esters as well as autoinducer-2 (AI-2), a collective term for a group of interconvertible furanones derived from dihydroxypentanedione (DPD) (Fig. 1). AI-2 is also produced by some Gram-positive bacteria, although generally these organisms prefer l ...
Lipid-Protein Bio-Nanotubes with Open or Closed Ends
... closed ends with lipid caps. Because the main governing concepts for this self assembly are general(13, 14) we expect synthetic analogs to follow similar assembly pathways, so that synthetic versions mimicking the LPNs (e.g. replacing microtubules with rigid polyelectrolytes) may have applications i ...
... closed ends with lipid caps. Because the main governing concepts for this self assembly are general(13, 14) we expect synthetic analogs to follow similar assembly pathways, so that synthetic versions mimicking the LPNs (e.g. replacing microtubules with rigid polyelectrolytes) may have applications i ...
Two faces of the prokaryote concept
... emphasized that bacteria and chromacae (blue-green algae) were, however, true to the definition [21]. “The whole vital activity of the simplest monera”, he wrote, “especially the chromacae is confined to their metabolism, and is therefore a purely chemical process, that may be compared to the cataly ...
... emphasized that bacteria and chromacae (blue-green algae) were, however, true to the definition [21]. “The whole vital activity of the simplest monera”, he wrote, “especially the chromacae is confined to their metabolism, and is therefore a purely chemical process, that may be compared to the cataly ...
A Genome-Wide Screen for Bacterial Envelope Biogenesis Mutants
... significantly in recent years with the emergence of carbapenemresistant Gram-negative Enterobacteriaceae like Klebsiella pneumoniae and Escherichia coli [10]. It is therefore important that new vulnerabilities in the Gram-negative envelope be identified to serve as targets for antibacterial drugs, o ...
... significantly in recent years with the emergence of carbapenemresistant Gram-negative Enterobacteriaceae like Klebsiella pneumoniae and Escherichia coli [10]. It is therefore important that new vulnerabilities in the Gram-negative envelope be identified to serve as targets for antibacterial drugs, o ...
Structure of Spin-Coated Lipid Films and Domain Formation in
... Upon hydration in liquid water, the multilamellar configuration was shown to be unstable and detach from the substrate except for one membrane remaining in the substrate. In this work, AFM and fluorescence microscopy is used to study the detailed structure of spin-coated lipid films before and after ...
... Upon hydration in liquid water, the multilamellar configuration was shown to be unstable and detach from the substrate except for one membrane remaining in the substrate. In this work, AFM and fluorescence microscopy is used to study the detailed structure of spin-coated lipid films before and after ...
Chemotaxis Movement and Attachment of Agrobacterium
... By incorporating potential attractants into minimal swarm plates, it is possible to perform crude tests of their attractiveness. This does usually require that the attractant can also be metabolized. ...
... By incorporating potential attractants into minimal swarm plates, it is possible to perform crude tests of their attractiveness. This does usually require that the attractant can also be metabolized. ...
Biologically Induced Mineralization by Bacteria
... constructed of phospholipid and its outer face contains lipopolysaccharide (LPS) which is highly anionic. LPS consists of O-polysaccharide, the core polysaccharide, and lipid A. The O-sidechain can extend up to 40 nm away from the core polysaccharide which is attached to lipid A. Lipid A contains se ...
... constructed of phospholipid and its outer face contains lipopolysaccharide (LPS) which is highly anionic. LPS consists of O-polysaccharide, the core polysaccharide, and lipid A. The O-sidechain can extend up to 40 nm away from the core polysaccharide which is attached to lipid A. Lipid A contains se ...
THE COMPLEMENT SYSTEM IN HUMAN DISEASE Joseph C. …
... In the absence if C1 inhibitor there is spontaneous activation of the classical complement pathway with cleavage of C4 and C2. Since there is no target cell surface for complement binding, C3 cleavage does not occur to any significant degree and if some C3b is formed, it undergoes spontaneous hydrol ...
... In the absence if C1 inhibitor there is spontaneous activation of the classical complement pathway with cleavage of C4 and C2. Since there is no target cell surface for complement binding, C3 cleavage does not occur to any significant degree and if some C3b is formed, it undergoes spontaneous hydrol ...
There are technologies emerging for the rapid detection of E. coli in
... DNA polymerase (Taq polymerase). The cycling of PCR results in an exponential amplication of the amount of the target sequence and thereby significantly increases the chance of detecting low numbers of target organisms in a sample (Bej et al. 1990). In order to detect the target sequence from an env ...
... DNA polymerase (Taq polymerase). The cycling of PCR results in an exponential amplication of the amount of the target sequence and thereby significantly increases the chance of detecting low numbers of target organisms in a sample (Bej et al. 1990). In order to detect the target sequence from an env ...
Atomic force microscopy study on specificity and non
... Enterococcus faecalis is one of the leading causes of hospital-acquired infections, and indwelling medical devices are especially prone to infection. E. faecalis expressing aggregation substance (Agg) adheres to biomaterial surfaces by means of positive cooperativity, i.e. the ability of one adherin ...
... Enterococcus faecalis is one of the leading causes of hospital-acquired infections, and indwelling medical devices are especially prone to infection. E. faecalis expressing aggregation substance (Agg) adheres to biomaterial surfaces by means of positive cooperativity, i.e. the ability of one adherin ...
Membranes 1: Lipids and Lipid Bilayers
... – paracrine hormones (locally acting) – all derived from arachidonic acid (20-carbon fatty acid with 4 double bonds) removed by phospholipase A2 – prostaglandins: mediate fever, inflammation and pain, among other functions – thromboxanes (involved in blood clotting) – leukotrienes (smooth muscle con ...
... – paracrine hormones (locally acting) – all derived from arachidonic acid (20-carbon fatty acid with 4 double bonds) removed by phospholipase A2 – prostaglandins: mediate fever, inflammation and pain, among other functions – thromboxanes (involved in blood clotting) – leukotrienes (smooth muscle con ...
Membranes 1: Lipids and Lipid Bilayers
... – paracrine hormones (locally acting) – all derived from arachidonic acid (20-carbon fatty acid with 4 double bonds) removed by phospholipase A2 – prostaglandins: mediate fever, fever inflammation and pain, pain among other functions – thromboxanes (involved in blood clotting) – leukotrienes (smooth ...
... – paracrine hormones (locally acting) – all derived from arachidonic acid (20-carbon fatty acid with 4 double bonds) removed by phospholipase A2 – prostaglandins: mediate fever, fever inflammation and pain, pain among other functions – thromboxanes (involved in blood clotting) – leukotrienes (smooth ...
Rapid Determination of Pathogenic Bacteria in Surface Waters
... DNA polymerase (Taq polymerase). The cycling of PCR results in an exponential amplication of the amount of the target sequence and thereby significantly increases the chance of detecting low numbers of target organisms in a sample (Bej et al. 1990). In order to detect the target sequence from an env ...
... DNA polymerase (Taq polymerase). The cycling of PCR results in an exponential amplication of the amount of the target sequence and thereby significantly increases the chance of detecting low numbers of target organisms in a sample (Bej et al. 1990). In order to detect the target sequence from an env ...
Base composition bias might result from competition for metabolic
... bacterial cell invasion, followed by cell division inhibition and phage replication up to depletion of bacterial resources [a]. These phages only replicate horizontally and replication is typically followed by bacterial lysis. Temperate and filamentous phages are different in this respect. After inv ...
... bacterial cell invasion, followed by cell division inhibition and phage replication up to depletion of bacterial resources [a]. These phages only replicate horizontally and replication is typically followed by bacterial lysis. Temperate and filamentous phages are different in this respect. After inv ...
High Resolution Structure of the Neisseria gonorrhoeae Type IV
... aeruginosa, and a truncated structure lacking the hydrophobic N-terminus was determined for Vibrio cholerae 2, 3. In a recently published article in Molecular Cell, this group report the 2.3 Å structure of N. gonorrhoeae (GC) pilin along with a “pseudo-atomic” structure of the pilus filament, solved ...
... aeruginosa, and a truncated structure lacking the hydrophobic N-terminus was determined for Vibrio cholerae 2, 3. In a recently published article in Molecular Cell, this group report the 2.3 Å structure of N. gonorrhoeae (GC) pilin along with a “pseudo-atomic” structure of the pilus filament, solved ...
Effects of Single Amino Acid Substitution on the Biophysical
... hydrophobicity. The amino acid sequences of the analogs are shown in Table 1. As the central location substitutions had great effects on the properties of peptides [12,19], the amino acid substitutions at the sixteenth position should maximize the effect of single amino acid substitutions. It is not ...
... hydrophobicity. The amino acid sequences of the analogs are shown in Table 1. As the central location substitutions had great effects on the properties of peptides [12,19], the amino acid substitutions at the sixteenth position should maximize the effect of single amino acid substitutions. It is not ...
Mechanisms associated with phagocytosis of
... Data were analyzed using Student’s t test through Graph Pad software. Values of p<0.05 were considered statistically significant. For the attachment inhibitory assay, results are shown as graphs indicating the count of attached bacteria to the amoeba (log CFU/mL) with and without monosaccharide trea ...
... Data were analyzed using Student’s t test through Graph Pad software. Values of p<0.05 were considered statistically significant. For the attachment inhibitory assay, results are shown as graphs indicating the count of attached bacteria to the amoeba (log CFU/mL) with and without monosaccharide trea ...
Lipopolysaccharide
Lipopolysaccharides (LPS), also known as lipoglycans and endotoxin, are large molecules consisting of a lipid and a polysaccharide composed of O-antigen, outer core and inner core joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria, and elicit strong immune responses in animals.The term lipooligosaccharide (""LOS"") is used to refer to a low molecular weight form of bacterial lipopolysaccharides.