Modeling Spatial Correlation of DNA Deformation
... ABSTRACT: We report a study of DNA deformations using a coarsegrained mechanical model and quantitatively interpret the allosteric effects in protein−DNA binding affinity. A recent single-molecule study (Kim et al. Science 2013, 339, 816) showed that when a DNA molecule is deformed by specific binding o ...
... ABSTRACT: We report a study of DNA deformations using a coarsegrained mechanical model and quantitatively interpret the allosteric effects in protein−DNA binding affinity. A recent single-molecule study (Kim et al. Science 2013, 339, 816) showed that when a DNA molecule is deformed by specific binding o ...
Phenotypic effects and variations in the genetic material (part 1)
... product becomes a disomic strain directly. The abortion patterns themselves are diagnostic for the presence of disomics in the asci. b. Trisomics – (2n+1) the gain of an extra copy of a chromosome so, the individual will have 3 copies of a certain chromosome. Nondisjunction is a failure of this disj ...
... product becomes a disomic strain directly. The abortion patterns themselves are diagnostic for the presence of disomics in the asci. b. Trisomics – (2n+1) the gain of an extra copy of a chromosome so, the individual will have 3 copies of a certain chromosome. Nondisjunction is a failure of this disj ...
B. Eukaryotic RNA polymerases
... (3) Termination does not occur in mutants lacking the repeat b) High G+C content (1) Near (or within) the stem loop sequence (2) Slows RNA polymerase down (a) Harder to break G-C bonds in DNA c) G-C region followed by A-T rich segment (1) Yields a RNA molecule with 5-6 Us (poly-U tail) (2) RNA polym ...
... (3) Termination does not occur in mutants lacking the repeat b) High G+C content (1) Near (or within) the stem loop sequence (2) Slows RNA polymerase down (a) Harder to break G-C bonds in DNA c) G-C region followed by A-T rich segment (1) Yields a RNA molecule with 5-6 Us (poly-U tail) (2) RNA polym ...
Stability of triple helices containing RNA and DNA strands
... to form a triple helix. Triple helix-forming oligonucleotides can compete with the binding of transcription factors (13 -15) and affect transcription initiation (15-17) or elongation (18,19). A triple helix approach can also be used to target a single-stranded nucleic acid, using either a linear oli ...
... to form a triple helix. Triple helix-forming oligonucleotides can compete with the binding of transcription factors (13 -15) and affect transcription initiation (15-17) or elongation (18,19). A triple helix approach can also be used to target a single-stranded nucleic acid, using either a linear oli ...
DNA structure 2008
... form between A and T, while three form between G and C. The bases can pair in this way only if the two polynucleotide chains that contain them are antiparallel to each other. The coiling of the two strands around each other creates two groves in the double helix. Consequences – each strand of DNA co ...
... form between A and T, while three form between G and C. The bases can pair in this way only if the two polynucleotide chains that contain them are antiparallel to each other. The coiling of the two strands around each other creates two groves in the double helix. Consequences – each strand of DNA co ...
1 Introduction
... localization signals (NLS), spanning amino acids 1422-1489 or 14541497, have been identified in etoposide-resistant cell lines (Takano and Fojo, 1995). At present, these mutations in NLS sequences are only found in IIα isoforms, and consequently the enzyme is extra-nuclear (Feldhoff et al, 1994; Mir ...
... localization signals (NLS), spanning amino acids 1422-1489 or 14541497, have been identified in etoposide-resistant cell lines (Takano and Fojo, 1995). At present, these mutations in NLS sequences are only found in IIα isoforms, and consequently the enzyme is extra-nuclear (Feldhoff et al, 1994; Mir ...
Human karyotype
... female has twice the genetic material than males for genes on the X chromosome dosage compensation: adjust for levels of gene expression in humans and many organisms dosage compensation occurs by X-inactivation the X-chromosome inactivated in each cell is randomly chosen can observe inacti ...
... female has twice the genetic material than males for genes on the X chromosome dosage compensation: adjust for levels of gene expression in humans and many organisms dosage compensation occurs by X-inactivation the X-chromosome inactivated in each cell is randomly chosen can observe inacti ...
Paper I- Discussion Points
... We also verify the above results by looking at the actual replication timings of the two fluorescent foci, one cyan (red) and the other green in a population of cells going through the cell cycle synchronously after release from G1. We simply classify cells into different classes. Class I: the green ...
... We also verify the above results by looking at the actual replication timings of the two fluorescent foci, one cyan (red) and the other green in a population of cells going through the cell cycle synchronously after release from G1. We simply classify cells into different classes. Class I: the green ...
The cytogenetics of homologous chromosome pairing in meiosis in plants Meiosis
... meiotic prophase I and coincides with two other major meiotic processes: recombination and synapsis. Recombination starts by formation of double-strand breaks (DSBs) in chromosomal DNA, which are later repaired, leading to crossovers between a single sister chromatid of each homologous chromosome. S ...
... meiotic prophase I and coincides with two other major meiotic processes: recombination and synapsis. Recombination starts by formation of double-strand breaks (DSBs) in chromosomal DNA, which are later repaired, leading to crossovers between a single sister chromatid of each homologous chromosome. S ...
The cytogenetics of homologous chromosome pairing in meiosis in
... meiotic prophase I and coincides with two other major meiotic processes: recombination and synapsis. Recombination starts by formation of double-strand breaks (DSBs) in chromosomal DNA, which are later repaired, leading to crossovers between a single sister chromatid of each homologous chromosome. S ...
... meiotic prophase I and coincides with two other major meiotic processes: recombination and synapsis. Recombination starts by formation of double-strand breaks (DSBs) in chromosomal DNA, which are later repaired, leading to crossovers between a single sister chromatid of each homologous chromosome. S ...
What Are Chromosomes?
... Normal Chromosomes Normal human somatic cells have 46 chromosomes: 22 pairs, or homologs, of autosomes (chromosomes 1-22) and two sex chromosomes. This is called the diploid number. Females carry two X chromosomes (46,XX) while males have an X and a Y (46,XY). Germ cells (egg and sperm) have 23 ...
... Normal Chromosomes Normal human somatic cells have 46 chromosomes: 22 pairs, or homologs, of autosomes (chromosomes 1-22) and two sex chromosomes. This is called the diploid number. Females carry two X chromosomes (46,XX) while males have an X and a Y (46,XY). Germ cells (egg and sperm) have 23 ...
Lecture Notes
... (a) Usually do not affect the individual, but may interfere with chromosome pairing during meiosis: a cause of multiple spontaneous abortions or abnormal liveborns. (b) Pericentric inversions include the centromere. (c) Paracentric inversions do not include the centromere. 5) Translocations: Breaks ...
... (a) Usually do not affect the individual, but may interfere with chromosome pairing during meiosis: a cause of multiple spontaneous abortions or abnormal liveborns. (b) Pericentric inversions include the centromere. (c) Paracentric inversions do not include the centromere. 5) Translocations: Breaks ...
Chromosomes in Saccharomyces cerevisiae
... Directed chromosome breakage. One possible explanation for the failure of artificial chromosomes to segregate as faithfully as their natural counterparts is that they carry telomeres derived from those of the Tetrahymena ribosomal DNA plasmid (Tr ends [24]) rather than those found on natural yeast c ...
... Directed chromosome breakage. One possible explanation for the failure of artificial chromosomes to segregate as faithfully as their natural counterparts is that they carry telomeres derived from those of the Tetrahymena ribosomal DNA plasmid (Tr ends [24]) rather than those found on natural yeast c ...
The nucleotide sequence of Saccharomyces cerevisiae
... Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-c ...
... Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-c ...
Chemistry and biology of DNA-binding small
... DNA-binding affinity, and cellular and nuclear transport activity, but also would not interfere with the functions of normal cells. Keywords: DNA-binding molecules, duplex DNA, G-quadruplex structures, transcription. ...
... DNA-binding affinity, and cellular and nuclear transport activity, but also would not interfere with the functions of normal cells. Keywords: DNA-binding molecules, duplex DNA, G-quadruplex structures, transcription. ...
The Influence of Hydrogen Donors on Breakage of Parental DNA
... reasoning is supported by the results obtained with of donor DNA. For this reason the transformation native DNA. It was found that double strand breaks experiments were repeated with another hydrogen with a linear dose dependency occur in hybrid as donor (mercaptoethanol ...
... reasoning is supported by the results obtained with of donor DNA. For this reason the transformation native DNA. It was found that double strand breaks experiments were repeated with another hydrogen with a linear dose dependency occur in hybrid as donor (mercaptoethanol ...
Exporter la page en pdf
... consist of megabase-scale domains of coordinated origin firing separated by large originless transition regions. Here, we report a quantitative genome-wide analysis of DNA replication kinetics in several human cell types that contradicts this view. DNA combing in HeLa cells sorted into four temporal ...
... consist of megabase-scale domains of coordinated origin firing separated by large originless transition regions. Here, we report a quantitative genome-wide analysis of DNA replication kinetics in several human cell types that contradicts this view. DNA combing in HeLa cells sorted into four temporal ...
The Relationship Between DNA Replication and the
... expected if they contained chromosomes in different stages of replication. In view of these uncertainties about thymine-requiring strains, we decided to investigate the relationship between DNA replication and induction of sporulation in a strain that does not require thymine for growth. In addition ...
... expected if they contained chromosomes in different stages of replication. In view of these uncertainties about thymine-requiring strains, we decided to investigate the relationship between DNA replication and induction of sporulation in a strain that does not require thymine for growth. In addition ...
File
... karyotyping, or chromosome analysis, in humans became available at that time. These techniques allow for the detection of normal vs. abnormal chromosome content in tested cells. Thus, karyotyping has become an important process in studying actual or potential birth defects due to chromosome abnormal ...
... karyotyping, or chromosome analysis, in humans became available at that time. These techniques allow for the detection of normal vs. abnormal chromosome content in tested cells. Thus, karyotyping has become an important process in studying actual or potential birth defects due to chromosome abnormal ...
Slides
... Section 18.1: Genetic Information: Replication §Telemeres– mini-satellite sequences at end of linear chromosome §Telomerase – overcomes susceptibility to nuclease digestion §Ribonucleoprotein with reverse transcriptase activity §RNA base sequence complementary to the TG-rich sequence of telomeres § ...
... Section 18.1: Genetic Information: Replication §Telemeres– mini-satellite sequences at end of linear chromosome §Telomerase – overcomes susceptibility to nuclease digestion §Ribonucleoprotein with reverse transcriptase activity §RNA base sequence complementary to the TG-rich sequence of telomeres § ...
pdf, 1.3 MB - DNA and Natural Algorithms Group
... Feng et al. 2003) have been constructed. For a recent review see Seeman (2003). This suggests that DNA may be a suitable medium for the construction of synthetic replicators. Most of the DNA-based nanomachines that have been constructed (Yurke et al. 2000; Simmel and Yurke 2001; Li and Tan 2002; Sim ...
... Feng et al. 2003) have been constructed. For a recent review see Seeman (2003). This suggests that DNA may be a suitable medium for the construction of synthetic replicators. Most of the DNA-based nanomachines that have been constructed (Yurke et al. 2000; Simmel and Yurke 2001; Li and Tan 2002; Sim ...
Cytogenetics Cytogenetics
... "land-mark" bands, and bands numbered sequentially within each. Sub-bands are catered for by using a decimal system ...
... "land-mark" bands, and bands numbered sequentially within each. Sub-bands are catered for by using a decimal system ...
Relationship between chromosome fragility, aneuploidy and
... repair as they show abnormal rearrangements associated with V(D)J recombination [10] and impaired fidelity in blunt DNA end-joining [11,12]. One of the major consequences of this defect is increased chromosome fragility especially after the treatments with DNA cross-linking agents. Also resembling o ...
... repair as they show abnormal rearrangements associated with V(D)J recombination [10] and impaired fidelity in blunt DNA end-joining [11,12]. One of the major consequences of this defect is increased chromosome fragility especially after the treatments with DNA cross-linking agents. Also resembling o ...
Processivity of DNA polymerases: two mechanisms, one goal
... as a processivity factor in a similar manner to T7. Similarly, the DNA polymerase of the Bacillus subtilis bacteriophage Spo1 also contains an insertion of 45 amino acids between α helices H and H1 [15]. This region is shorter than the one found in T3 and T7 and does not have significant similaritie ...
... as a processivity factor in a similar manner to T7. Similarly, the DNA polymerase of the Bacillus subtilis bacteriophage Spo1 also contains an insertion of 45 amino acids between α helices H and H1 [15]. This region is shorter than the one found in T3 and T7 and does not have significant similaritie ...
The replication of DNA
... placement of sliding camp on DNA. These enzyme couple ATP binding and hydrolysis to the placement of sliding clamp around primer template junction, every time that this junction is present in the cell. The clamp loaders also remove the slide clamp from DNA once all of the enzymes that interact with ...
... placement of sliding camp on DNA. These enzyme couple ATP binding and hydrolysis to the placement of sliding clamp around primer template junction, every time that this junction is present in the cell. The clamp loaders also remove the slide clamp from DNA once all of the enzymes that interact with ...
Telomere
A telomere is a region of repetitive nucleotide sequences at each end of a chromatid, which protects the end of the chromosome from deterioration or from fusion with neighboring chromosomes. Its name is derived from the Greek nouns telos (τέλος) 'end' and merοs (μέρος, root: μερ-) 'part.' For vertebrates, the sequence of nucleotides in telomeres is TTAGGG. This sequence of TTAGGG is repeated approximately 2,500 times in humans. During chromosome replication, the enzymes that duplicate DNA cannot continue their duplication all the way to the end of a chromosome, so in each duplication the end of the chromosome is shortened (this is because the synthesis of Okazaki fragments requires RNA primers attaching ahead on the lagging strand). The telomeres are disposable buffers at the ends of chromosomes which are truncated during cell division; their presence protects the genes before them on the chromosome from being truncated instead.Over time, due to each cell division, the telomere ends become shorter. They are replenished by an enzyme, telomerase reverse transcriptase.