DNA Metallization Processes and Nanoelectronics
... insertion of the metal complexes between the DNA bases (like platinum or palladium complexes [14]). In the second step, the bound metal ions or metal complexes are usually treated with a reducing agent (Fig. 1b). This converts them in metal nanoclusters fixed on the DNA strand. The more used reducing ...
... insertion of the metal complexes between the DNA bases (like platinum or palladium complexes [14]). In the second step, the bound metal ions or metal complexes are usually treated with a reducing agent (Fig. 1b). This converts them in metal nanoclusters fixed on the DNA strand. The more used reducing ...
dna structure flip
... discovery, you are prepared for your mission: To determine how cells make new copies of their DNA. Remember, it is still the 1950s. At this time, three hypothetical models have been proposed to explain how DNA is replicated. These models, known as the conservative model, the dispersive model, and th ...
... discovery, you are prepared for your mission: To determine how cells make new copies of their DNA. Remember, it is still the 1950s. At this time, three hypothetical models have been proposed to explain how DNA is replicated. These models, known as the conservative model, the dispersive model, and th ...
Characterization of Deletions in the LDL Receptor Gene in Patients
... a clinical diagnosis of FH who reside in the southeast of England and in whom we intend to determine the frequency of the previously described mutations in the LDL receptor gene. In this article we describe the identification of those patients in the group in whom the mutant allele of the LDL recept ...
... a clinical diagnosis of FH who reside in the southeast of England and in whom we intend to determine the frequency of the previously described mutations in the LDL receptor gene. In this article we describe the identification of those patients in the group in whom the mutant allele of the LDL recept ...
101. The Role of Rigidity in DNA Looping
... protein prefers binding to the well separated I1 and O2 half-sites and forming a DNA loop (1–4), Fig. 1. On the addition of arabinose, the protein’s affinity for the I1 and I2 half-sites increases by about 50-fold, leading the protein to prefer to bind to these adjacent half-sites and induce the pBA ...
... protein prefers binding to the well separated I1 and O2 half-sites and forming a DNA loop (1–4), Fig. 1. On the addition of arabinose, the protein’s affinity for the I1 and I2 half-sites increases by about 50-fold, leading the protein to prefer to bind to these adjacent half-sites and induce the pBA ...
The Role of Sperm Chromatin Integrity and DNA Damage on Male
... Base modifications are the second most prevalent form of spermatozoa DNA damage after strand breaks. These base alterations result in improper reading of the base sequences, and incorrect protein production. These changes are due to either additions to the base sequence or subtraction of the necessa ...
... Base modifications are the second most prevalent form of spermatozoa DNA damage after strand breaks. These base alterations result in improper reading of the base sequences, and incorrect protein production. These changes are due to either additions to the base sequence or subtraction of the necessa ...
Number 53, 2006 5 m sh4
... A 3.2 kb sequence including the potential msh4 gene was analysed using GenScan (Burge and Karlin, 1997) to identify possible coding sequence and to suggest possible amino acid sequences by removing introns predicted by identification of eukaryotic splice sites (figure 2). Using either human or Arabi ...
... A 3.2 kb sequence including the potential msh4 gene was analysed using GenScan (Burge and Karlin, 1997) to identify possible coding sequence and to suggest possible amino acid sequences by removing introns predicted by identification of eukaryotic splice sites (figure 2). Using either human or Arabi ...
On Map Representations of DNA†
... having about 160 amino acids, takes about 15 minutes by using only pen and pencil (no calculator or computer). The high efficiency of the exact solution is due to the fact that the algorithm identifies also pairs of adjacent amino which appear only in one sequence and not in the other, and thus elim ...
... having about 160 amino acids, takes about 15 minutes by using only pen and pencil (no calculator or computer). The high efficiency of the exact solution is due to the fact that the algorithm identifies also pairs of adjacent amino which appear only in one sequence and not in the other, and thus elim ...
evolution of protein function by domain swapping
... The study and understanding of the natural evolution of protein function clearly has important implications for the design of in vitro evolution strategies. Because nature is blind to the rules and relationships of sequence, structure, and function, strategies that seem most applicable are combinato ...
... The study and understanding of the natural evolution of protein function clearly has important implications for the design of in vitro evolution strategies. Because nature is blind to the rules and relationships of sequence, structure, and function, strategies that seem most applicable are combinato ...
Nucleotide sequence of the thioredoxin gene from
... gene~ a 15-mer oligodeoxyribonucleotide was synthesized (Table 1) for use as a primer in the sequence analysis. The isolated 3-kb fragment was used as template in direct dideoxy sequence analysis after heat-induced strand separation. The mixed l#-mer oligodeoxyribonucleotide was utilized as primer t ...
... gene~ a 15-mer oligodeoxyribonucleotide was synthesized (Table 1) for use as a primer in the sequence analysis. The isolated 3-kb fragment was used as template in direct dideoxy sequence analysis after heat-induced strand separation. The mixed l#-mer oligodeoxyribonucleotide was utilized as primer t ...
Using a Single-Nucleotide Polymorphism to Predict
... of many drugs. For example, SNPs in serotonin transporter and receptor genes predict adverse responses to anti-depression drugs, including PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds conta ...
... of many drugs. For example, SNPs in serotonin transporter and receptor genes predict adverse responses to anti-depression drugs, including PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds conta ...
DNA PROVIDER bro.indd - the National Center for Victims of Crime
... of material in which DNA can be found are blood, saliva, sweat, urine, skin tissue, ...
... of material in which DNA can be found are blood, saliva, sweat, urine, skin tissue, ...
Structural Basis of Transcription Initiation: An RNA
... includes the –35 element, is straight (Fig. 2B). In addition, the 4 recognition helix is shifted upstream about 6 Å, so that the sequence-specific interactions with the –35 element (9) could not occur. There are two possible explanations for this discrepancy between the high-resolution 4-DNA struc ...
... includes the –35 element, is straight (Fig. 2B). In addition, the 4 recognition helix is shifted upstream about 6 Å, so that the sequence-specific interactions with the –35 element (9) could not occur. There are two possible explanations for this discrepancy between the high-resolution 4-DNA struc ...
Leishmania donovani - Oxford Academic
... topoisomerase I open reading frame from the kinetoplastid Leishmania donovani was reported which shared a substantial degree of homology with type IB topoisomerases but having a variable C-terminus. Here we present a molecular model of the above parasite gene product, using the human topoisomerase I ...
... topoisomerase I open reading frame from the kinetoplastid Leishmania donovani was reported which shared a substantial degree of homology with type IB topoisomerases but having a variable C-terminus. Here we present a molecular model of the above parasite gene product, using the human topoisomerase I ...
Jounral of Bacteriology
... pathogenic organisms present in the rhizosphere. We sampled sequenced the genome of FZB42 and identified 2,947 genes with >50% identity on the amino acid level to the corresponding genes of Bacillus subtilis 168. Six large gene clusters encoding nonribosomal peptide synthetases (NRPS) and polyketide ...
... pathogenic organisms present in the rhizosphere. We sampled sequenced the genome of FZB42 and identified 2,947 genes with >50% identity on the amino acid level to the corresponding genes of Bacillus subtilis 168. Six large gene clusters encoding nonribosomal peptide synthetases (NRPS) and polyketide ...
Interaction of Rat Testis Protein, TP, with Nucleic Acids in Vitro
... respectively. It is clear that TP did induce hyperchromicity of both DNA (Fig. 8A) and poly(rA) (Fig. 8B) at this salt concentration also, and the shapes of the difference spectra were very similar to those observed at 2 mM Na' concentration. Effect of TP Binding on the Thermal Melting of the DNAThe ...
... respectively. It is clear that TP did induce hyperchromicity of both DNA (Fig. 8A) and poly(rA) (Fig. 8B) at this salt concentration also, and the shapes of the difference spectra were very similar to those observed at 2 mM Na' concentration. Effect of TP Binding on the Thermal Melting of the DNAThe ...
View/Open
... organic chemistry thus far is related to how things work chemically, how diseases can be treated at the molecular level with small molecules, and how we can create new compounds and materials that improve our daily lives. One of the most interesting of the many applications of organic chemistry is ...
... organic chemistry thus far is related to how things work chemically, how diseases can be treated at the molecular level with small molecules, and how we can create new compounds and materials that improve our daily lives. One of the most interesting of the many applications of organic chemistry is ...
BCL-6, a POZ transcriptional repressor zinc-finger protein, is a sequence-specific C
... and that the full-length protein was present in the complex. Analogous EMSA performed on nuclear extracts from a panel of B cells representative of discrete stages of B-cell differentiation indicated complete concordance between formation of BCL-6 containing protein–DNA complexes and protein express ...
... and that the full-length protein was present in the complex. Analogous EMSA performed on nuclear extracts from a panel of B cells representative of discrete stages of B-cell differentiation indicated complete concordance between formation of BCL-6 containing protein–DNA complexes and protein express ...
First mutation in the red blood cell-specific
... was due to compound heterozygosity for a 95 bp deletion and a c.1667T>C missense substitution causing a p.Leu529Ser amino acid change. Kanno et al.11 described a homozygous 9490-bp intragenic deletion variant and, more recently, van Wijk et al.12 reported a homozygous missense mutation in exon 15 of ...
... was due to compound heterozygosity for a 95 bp deletion and a c.1667T>C missense substitution causing a p.Leu529Ser amino acid change. Kanno et al.11 described a homozygous 9490-bp intragenic deletion variant and, more recently, van Wijk et al.12 reported a homozygous missense mutation in exon 15 of ...
Putrescine oxidase of Micrococcus rubens : primary
... colonies, under the same conditions as for the above Southern hybridization. Plasmid DNA was prepared from the positive colonies by CsCl/ethidium bromide ultracentrifugation. Restriction endonuclease analysis together with Southern hybridization experiments with the probes indicated the location of ...
... colonies, under the same conditions as for the above Southern hybridization. Plasmid DNA was prepared from the positive colonies by CsCl/ethidium bromide ultracentrifugation. Restriction endonuclease analysis together with Southern hybridization experiments with the probes indicated the location of ...
Amplification of a DNA Fragment Using Polymerase
... (proofreading) activity. The Taq DNA polymerase has no proofreading activity, increasing the possibility of introducing point mutations (single base pair changes) in the amplified DNA product. In this experiment, you will amplify a fragment of pBluescript II (a plasmid), which includes the multiple ...
... (proofreading) activity. The Taq DNA polymerase has no proofreading activity, increasing the possibility of introducing point mutations (single base pair changes) in the amplified DNA product. In this experiment, you will amplify a fragment of pBluescript II (a plasmid), which includes the multiple ...
Alisch RS, Wang T, Chopra P, Visootsak J, Conneely KN, Warren ST . Genome-wide analysis validates aberrant methylation in fragile X syndrome is specific to the FMR1 locus. BMC Med Genet. 2013 Jan 29;14:18. doi: 10.1186/1471-2350-14-18.
... interfering RNA (siRNA) and generally causes the transcriptional downregulation of a genomic region [12]. This model is attractive in that the unmethylated full mutation allele is known to be expressed in early development, presumably producing a transcript with a long riboCGG tract, and this riboCG ...
... interfering RNA (siRNA) and generally causes the transcriptional downregulation of a genomic region [12]. This model is attractive in that the unmethylated full mutation allele is known to be expressed in early development, presumably producing a transcript with a long riboCGG tract, and this riboCG ...
BRCA2 Is Required for Homology-Directed Repair of Chromosomal
... expressed. Homologous integrations were also detected in the Brca2lex1/lex2 ES cells (64 targeted clones/121 total), but at an approximately 1.8-fold lower frequency. This diminished ability to gene target is suggestive of a homologous recombination defect in cells that are mutated for the Brca2 gen ...
... expressed. Homologous integrations were also detected in the Brca2lex1/lex2 ES cells (64 targeted clones/121 total), but at an approximately 1.8-fold lower frequency. This diminished ability to gene target is suggestive of a homologous recombination defect in cells that are mutated for the Brca2 gen ...
View
... The positioning patterns of γ-H2AX in Jurkat cells indicate that sub-telomeric and actively transcribed regions are sensitive to endogenous DNA damage. Sub-telomeres are known to be prone to replication-mediated DSBs, particularly due to oncogenic replication stress (10,11). DNA hyperreplication in ...
... The positioning patterns of γ-H2AX in Jurkat cells indicate that sub-telomeric and actively transcribed regions are sensitive to endogenous DNA damage. Sub-telomeres are known to be prone to replication-mediated DSBs, particularly due to oncogenic replication stress (10,11). DNA hyperreplication in ...
UCSF Community Viral Load
... treatment and support services at the heart of the effort to improve the health outcomes of HIV+ individuals and prevent new infections.6 What is the scientific basis for this approach, how might it actually be implemented, and will it have the desired results in the real world? ...
... treatment and support services at the heart of the effort to improve the health outcomes of HIV+ individuals and prevent new infections.6 What is the scientific basis for this approach, how might it actually be implemented, and will it have the desired results in the real world? ...