Analyzing the Changes in DNA Flexibility Due to Base Modifications
... Unlike protein simulations for which one usually starts MD simulations using an experimentally determined native structure, the native structure of a given DNA sequence can rarely be found except for several well known DNA sequences. Thus, we build a dsDNA helix with a canonical B-DNA conformation u ...
... Unlike protein simulations for which one usually starts MD simulations using an experimentally determined native structure, the native structure of a given DNA sequence can rarely be found except for several well known DNA sequences. Thus, we build a dsDNA helix with a canonical B-DNA conformation u ...
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.
... Yeast vectors are of four general types. These differ in the manner in which they are maintained in yeast cells. YIp (yeast integrating plasmid) vectors lack a yeast replication origin, so must be propagated as integrated elements in a yeast chromosome, usually in a single copy per genome. YRp (yeas ...
... Yeast vectors are of four general types. These differ in the manner in which they are maintained in yeast cells. YIp (yeast integrating plasmid) vectors lack a yeast replication origin, so must be propagated as integrated elements in a yeast chromosome, usually in a single copy per genome. YRp (yeas ...
Altering substrate specificity of catechol 2,3
... C23O for tested catecholic compounds is shown in Table 1. The lowest activity towards catechol and catechols with substituent in para-position (4-chlorocatechol, 4-methylcatechol) was found for mutant C23OB58 (Table 1). In this mutant three amino acid changes were localized: H24R, F168S, Q275R. Base ...
... C23O for tested catecholic compounds is shown in Table 1. The lowest activity towards catechol and catechols with substituent in para-position (4-chlorocatechol, 4-methylcatechol) was found for mutant C23OB58 (Table 1). In this mutant three amino acid changes were localized: H24R, F168S, Q275R. Base ...
Tracking bacterial DNA replication forks in vivo by pulsed field gel
... Intact chromosomal DNA was prepared in agarose and digested with the restriction enzyme Not I. The resulting Not I fragments were fractionated by PFG electrophoresis. Exposure of this gel to X-ray film revealed the time-dependent incorporation of 14C-thymidine into various Not I fragments (Figure 2) ...
... Intact chromosomal DNA was prepared in agarose and digested with the restriction enzyme Not I. The resulting Not I fragments were fractionated by PFG electrophoresis. Exposure of this gel to X-ray film revealed the time-dependent incorporation of 14C-thymidine into various Not I fragments (Figure 2) ...
Alteration by site-directed mutagenesis of the
... nuclease activity on double-stranded [3H]DNA was measured as in (11). Table I shows the nuclease activity on double-stranded DNA found in each cell extract. There is a significant amount of ATP-stimulated nuclease activity on double-stranded DNA in crude extracts of cells expressing the wild-type ge ...
... nuclease activity on double-stranded [3H]DNA was measured as in (11). Table I shows the nuclease activity on double-stranded DNA found in each cell extract. There is a significant amount of ATP-stimulated nuclease activity on double-stranded DNA in crude extracts of cells expressing the wild-type ge ...
Development of a qPCR Method to Measure Mitochondrial
... by a range of genotoxic stimuli and interrogate specific DNA loci in both mitochondrial and genomic DNA with reproducible sensitivity. However, this method has not been widely tested on different instruments (the published report used Roche LightCycler 480 II instrument) and has the disadvantage of ...
... by a range of genotoxic stimuli and interrogate specific DNA loci in both mitochondrial and genomic DNA with reproducible sensitivity. However, this method has not been widely tested on different instruments (the published report used Roche LightCycler 480 II instrument) and has the disadvantage of ...
Sequence-specific RNA Photocleavage by Single
... gene suppression strategies through RNA degradation involving antisense oligonucleotides and artificial ribonucleases1–5. More efforts are still need to put into these methodologies to improve the efficiency before they could be broadly adapted as genomic or therapeutic tools. Since the first descri ...
... gene suppression strategies through RNA degradation involving antisense oligonucleotides and artificial ribonucleases1–5. More efforts are still need to put into these methodologies to improve the efficiency before they could be broadly adapted as genomic or therapeutic tools. Since the first descri ...
Conclusions Synapsin IIa is expressed in the brain of adult zebrafish
... Figure 1: RT-PCR analysis of Synapsin IIa from zebrafish embryos. The SynIIa gene was amplified from zebrafish brain, 24 hpf, and 72 hpf. The primers used encompassed the predicted ATG start codon and stop codon to produce an amplicon of 1420 base pairs ...
... Figure 1: RT-PCR analysis of Synapsin IIa from zebrafish embryos. The SynIIa gene was amplified from zebrafish brain, 24 hpf, and 72 hpf. The primers used encompassed the predicted ATG start codon and stop codon to produce an amplicon of 1420 base pairs ...
12–4 Mutations - Gravette School District
... of the proteins for which they code. Mutations that cause dramatic changes in protein structure or gene activity are often harmful, producing defective proteins that disrupt normal biological activities. However, mutations are also the source of genetic variability in a species. Some of this variati ...
... of the proteins for which they code. Mutations that cause dramatic changes in protein structure or gene activity are often harmful, producing defective proteins that disrupt normal biological activities. However, mutations are also the source of genetic variability in a species. Some of this variati ...
Structure, function and evolution of the XPD family of iron–sulfur
... The lack of phenotype of a DinG knockout may be explained by the existence of a second repair pathway with overlapping specificity. As shown in Figure 2, DinG in Gram-positive bacteria acquired a further function with the fusion of an exonuclease III-type domain at the N-terminus. This nuclease– hel ...
... The lack of phenotype of a DinG knockout may be explained by the existence of a second repair pathway with overlapping specificity. As shown in Figure 2, DinG in Gram-positive bacteria acquired a further function with the fusion of an exonuclease III-type domain at the N-terminus. This nuclease– hel ...
Reviews Cross-protection by anti
... Eijkman-Winkler Institute for Medical & Clinical Microbiology, bDepartment of Intensive Care & Clinical Toxicology and cDepartment of Medicine, Division of Infectious Diseases and AIDS, Utrecht University Hospital, The Netherlands The ability of antibodies against the core glycolipid (CGL) of endoto ...
... Eijkman-Winkler Institute for Medical & Clinical Microbiology, bDepartment of Intensive Care & Clinical Toxicology and cDepartment of Medicine, Division of Infectious Diseases and AIDS, Utrecht University Hospital, The Netherlands The ability of antibodies against the core glycolipid (CGL) of endoto ...
Database Mining Tools in the Human Genome Initiative
... context of higher order processes such as the regulation of gene expression, metabolic pathways and signalling cascades. Thus, the final objective of such higher-level functional analysis will be the elucidation of high-resolution structural and functional maps of the human genome. ...
... context of higher order processes such as the regulation of gene expression, metabolic pathways and signalling cascades. Thus, the final objective of such higher-level functional analysis will be the elucidation of high-resolution structural and functional maps of the human genome. ...
Ch12_lecture - Dr. Brahmbhatt`s Class Handouts
... soon after infection might cure the resulting disease much more rapidly than waiting for the immune system to handle the pathogens. • Plant-derived antibodies against bacteria that cause tooth decay and non-Hodgkins lymphoma could be produced cheaply, enhancing the availability of therapies. Copyrig ...
... soon after infection might cure the resulting disease much more rapidly than waiting for the immune system to handle the pathogens. • Plant-derived antibodies against bacteria that cause tooth decay and non-Hodgkins lymphoma could be produced cheaply, enhancing the availability of therapies. Copyrig ...
Characterization of the Human Gene for a Newly Discovered
... corresponding regions of the human CA I, CA II, and CA III proteins, respectively. This region of the carbonic anhydrases is precisely encoded by exon 6 of all other characterized vertebrate CA genes (Tashian et al., 1990; Hewett-Emmett and Tashian, 1991). Additional potential coding material, which ...
... corresponding regions of the human CA I, CA II, and CA III proteins, respectively. This region of the carbonic anhydrases is precisely encoded by exon 6 of all other characterized vertebrate CA genes (Tashian et al., 1990; Hewett-Emmett and Tashian, 1991). Additional potential coding material, which ...
The Mechanism of Insertion of a Segment of
... the B. suhtifis chromosome. It has generally been assumed that integration is by a Campbell mechanism, by analogy with the mechanism of integration of bacteriophage ;1 into the chromosome of E. cofi (Campbell, 1962). In some cases evidence has been adduced, principally from Southern hybridization ex ...
... the B. suhtifis chromosome. It has generally been assumed that integration is by a Campbell mechanism, by analogy with the mechanism of integration of bacteriophage ;1 into the chromosome of E. cofi (Campbell, 1962). In some cases evidence has been adduced, principally from Southern hybridization ex ...
File
... A) the leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction. B) the leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized b ...
... A) the leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction. B) the leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized b ...
Making sense of deep sequencing
... sequencing methods. A few misreads in a hundred can be dismissed. Redundancy is also necessary because of biology. RNA expression and epigenetic modifications are measured by counting sequence representations, quantitative accuracy depending on the number of representations. There is often wide varia ...
... sequencing methods. A few misreads in a hundred can be dismissed. Redundancy is also necessary because of biology. RNA expression and epigenetic modifications are measured by counting sequence representations, quantitative accuracy depending on the number of representations. There is often wide varia ...
A type III-like restriction endonuclease functions as a major barrier to
... cleavage occurs on collision of two translocating restriction complexes away from the recognition site (11). The type II restriction system is well known by all scientists who perform molecular biology experiments (12, 13). It consists of a site-specific methylase and a site-specific restriction endon ...
... cleavage occurs on collision of two translocating restriction complexes away from the recognition site (11). The type II restriction system is well known by all scientists who perform molecular biology experiments (12, 13). It consists of a site-specific methylase and a site-specific restriction endon ...
Analysis of aptamer sequence activity relationshipsw
... dissociation constants and the weight matrix scores.13,14 This results from a combination of noise within the SELEX process and over-selection of the best aptamers from each generation.13 It has been demonstrated that in order to construct an accurate model of DNA binding, information is required no ...
... dissociation constants and the weight matrix scores.13,14 This results from a combination of noise within the SELEX process and over-selection of the best aptamers from each generation.13 It has been demonstrated that in order to construct an accurate model of DNA binding, information is required no ...
How to determine whether a strain will undergo senescence. Background
... unlimited growth. Deleterious mutant genes and gene combinations are expected eventually to accumulate and slow or terminate growth even in normal growing, nonsenescent cultures. Occasional outcrossing can purge the genome of the accumulated defective genes, but in the absence of genetic recombinati ...
... unlimited growth. Deleterious mutant genes and gene combinations are expected eventually to accumulate and slow or terminate growth even in normal growing, nonsenescent cultures. Occasional outcrossing can purge the genome of the accumulated defective genes, but in the absence of genetic recombinati ...
Human Heredity - Lyndhurst School
... and one Y chromosome. As you can see in Figure 14–2, this is the reason why males and females are born in a roughly 50 : 50 ratio. All human egg cells carry a single X chromosome (23,X). However, half of all sperm cells carry an X chromosome (23,X) and half carry a Y chromosome (23,Y). This ensures ...
... and one Y chromosome. As you can see in Figure 14–2, this is the reason why males and females are born in a roughly 50 : 50 ratio. All human egg cells carry a single X chromosome (23,X). However, half of all sperm cells carry an X chromosome (23,X) and half carry a Y chromosome (23,Y). This ensures ...
![A second allele of spectrin alpha-gene associated with the alpha... phenotype (allele alpha Ponte de Sor) [letter]](http://s1.studyres.com/store/data/007858793_1-cec18df0f8a6bd226cd9c76efa32daa1-300x300.png)
A second allele of spectrin alpha-gene associated with the alpha... phenotype (allele alpha Ponte de Sor) [letter]
... mutation at position I5 1 . The latter created the same abnormal trypsin cleavage site after Arg 137. A limited elliptocytosis accompanied the al.lil.Y/aPa,ntC de SOr diplotype, but not the a""""dc '"/a diplotype. The modulation by allele assigned the primary alteration to spectrin a-gene. The norma ...
... mutation at position I5 1 . The latter created the same abnormal trypsin cleavage site after Arg 137. A limited elliptocytosis accompanied the al.lil.Y/aPa,ntC de SOr diplotype, but not the a""""dc '"/a diplotype. The modulation by allele assigned the primary alteration to spectrin a-gene. The norma ...
dna: the indispensible forensic science tool
... bands (one RFLP from each chromosome). • When comparing the DNA fragment patterns of two or more specimens, one merely looks for a match between the band sets. • A high degree of discrimination can be achieved by using a number of different probes and combining their frequencies. CRIMINALISTICS An I ...
... bands (one RFLP from each chromosome). • When comparing the DNA fragment patterns of two or more specimens, one merely looks for a match between the band sets. • A high degree of discrimination can be achieved by using a number of different probes and combining their frequencies. CRIMINALISTICS An I ...
MayerFrankiPoster
... the photosynthetic process requires phycobilisome (PBS) light-harvesting protein complexes that efficiently capture solar energy (Figure 2). Cell density and bioproduct formation in cyanobacterial cultures is limited by an ‘overshadowing’ effect. Cells growing at the surface of a culture capture mos ...
... the photosynthetic process requires phycobilisome (PBS) light-harvesting protein complexes that efficiently capture solar energy (Figure 2). Cell density and bioproduct formation in cyanobacterial cultures is limited by an ‘overshadowing’ effect. Cells growing at the surface of a culture capture mos ...
Polymerase Chain Reaction (PCR) - G
... look for the insertion of a short DNA sequence called Alu within the PV92 locus. DNA from different individuals contain many regions that exhibit a great deal of diversity and these regions are known as polymorphic (many forms) and provide the basis for genetic disease diagnosis, forensic identi ...
... look for the insertion of a short DNA sequence called Alu within the PV92 locus. DNA from different individuals contain many regions that exhibit a great deal of diversity and these regions are known as polymorphic (many forms) and provide the basis for genetic disease diagnosis, forensic identi ...