unit 7 exam study guide
... 15. What makes up the “backbone” of a DNA molecule? 16. What makes up the "rungs" of a DNA molecule? 17. What type of bonds holds the DNA bases together? 18. Explain Chargaff’s discovery. 19. If a DNA molecule contains 22% adenine, what percentages of the other bases would be present? 20. If the seq ...
... 15. What makes up the “backbone” of a DNA molecule? 16. What makes up the "rungs" of a DNA molecule? 17. What type of bonds holds the DNA bases together? 18. Explain Chargaff’s discovery. 19. If a DNA molecule contains 22% adenine, what percentages of the other bases would be present? 20. If the seq ...
CH 13 Gene Technoogy PPT
... In recombinant DNA, nucleotide sequences from two different sources, often two species, are combined in vitro into the same DNA molecule ...
... In recombinant DNA, nucleotide sequences from two different sources, often two species, are combined in vitro into the same DNA molecule ...
DNA – Deoxyribose Nucleic Acid
... Errors in DNA replication? Mutations can be in form of deletions, additions, and/or change of nucleotide types. Errors are usually very few – a type of DNA polymerase is involved in proof-reading and repair of mistakes. Final errors are usually about one in a billion. DNA polymerases and DNA ligase ...
... Errors in DNA replication? Mutations can be in form of deletions, additions, and/or change of nucleotide types. Errors are usually very few – a type of DNA polymerase is involved in proof-reading and repair of mistakes. Final errors are usually about one in a billion. DNA polymerases and DNA ligase ...
Understanding DNA Structure
... total length of DNA in a human cell is about 2m DNA is compacted in length by a factor of 10000 the compaction could be higher (if DNA was a ball of string) DNA of one chromosome is very long and narrow (expanded scale: length = 30km, diameter = 2mm. DNA carries genetic material ...
... total length of DNA in a human cell is about 2m DNA is compacted in length by a factor of 10000 the compaction could be higher (if DNA was a ball of string) DNA of one chromosome is very long and narrow (expanded scale: length = 30km, diameter = 2mm. DNA carries genetic material ...
DNA - The Double Helix
... construct a house, the DNA "blueprint" tells the cell how to build the organism. Yet, how can a heart be so different from a brain if all the cells contain the same instructions? Although much work remains in genetics, it has become apparent that a cell has the ability to turn off most genes and onl ...
... construct a house, the DNA "blueprint" tells the cell how to build the organism. Yet, how can a heart be so different from a brain if all the cells contain the same instructions? Although much work remains in genetics, it has become apparent that a cell has the ability to turn off most genes and onl ...
Biotechnology - The Bio Edge
... 42. The DNA of somatic cells is constantly bombarded with agents from the environment that could cause mutations. Select the correct statement about mutations and somatic cells. A. Somatic cells can withstand the mutations that might be induced since there are so many cell cycles in a somatic cell’s ...
... 42. The DNA of somatic cells is constantly bombarded with agents from the environment that could cause mutations. Select the correct statement about mutations and somatic cells. A. Somatic cells can withstand the mutations that might be induced since there are so many cell cycles in a somatic cell’s ...
CHAPTER 18
... occurred during DNA replication. In this case, the wrong base is in the newly made strand. The binding of MutH, which occurs on a hemimethylated sequence, provides a sensing mechanism to distinguish between the unmethylated and methylated strands. In other words, MutH binds to the hemimethylated DNA ...
... occurred during DNA replication. In this case, the wrong base is in the newly made strand. The binding of MutH, which occurs on a hemimethylated sequence, provides a sensing mechanism to distinguish between the unmethylated and methylated strands. In other words, MutH binds to the hemimethylated DNA ...
E. Coli - mrkeay
... • Plasmids carry genes which confer antibiotic resistance, as well as resistance to toxic heavy metals and industrial chemicals • We can use plasmids for biotechnology, since bacteria are able to express foreign genes inserted into plasmids ...
... • Plasmids carry genes which confer antibiotic resistance, as well as resistance to toxic heavy metals and industrial chemicals • We can use plasmids for biotechnology, since bacteria are able to express foreign genes inserted into plasmids ...
DNA Paternity Test RFLP analysis (Restriction Fragment Length
... sequences -each enzyme recognizes and cuts DNA at a different base sequence e.g. BamHI XXXXXXXXGGATCCXXXXXXXXXX XXXXXXXXCCTAGGXXXXXXXXXX -due to spontaneous mutations over time, different people have slightly different base sequences in their DNA -if mutation creates or deletes a restriction site in ...
... sequences -each enzyme recognizes and cuts DNA at a different base sequence e.g. BamHI XXXXXXXXGGATCCXXXXXXXXXX XXXXXXXXCCTAGGXXXXXXXXXX -due to spontaneous mutations over time, different people have slightly different base sequences in their DNA -if mutation creates or deletes a restriction site in ...
12_Clicker_Questions
... with λ DNA that was cut with both EcoR1 and BamH1 enzymes together. This creates several additional fragments. Which EcoR1 fragment does not have a BamH1 cut site? a. the first fragment from the top (the largest) b. the second fragment from the top c. the second to the bottom fragment d. the bottom ...
... with λ DNA that was cut with both EcoR1 and BamH1 enzymes together. This creates several additional fragments. Which EcoR1 fragment does not have a BamH1 cut site? a. the first fragment from the top (the largest) b. the second fragment from the top c. the second to the bottom fragment d. the bottom ...
Sos response in E. coli
... damages its DNA. For example: Radiation or some type of mutagen that will damage the bacterial DNA. After damage occurs lesions will appear damaging sections of the DNA. ...
... damages its DNA. For example: Radiation or some type of mutagen that will damage the bacterial DNA. After damage occurs lesions will appear damaging sections of the DNA. ...
Conceptual Questions C1. Answer: A. G→A, which is a transition. B
... C35. Answer: The DNA N-glycosylase would first make a cut between the base and the sugar. This would release the thymine base from the nucleotide to which it was directly attached, but the thymine would still be connected to the adjacent (3’) thymine. AP endonuclease would then make a nick in this D ...
... C35. Answer: The DNA N-glycosylase would first make a cut between the base and the sugar. This would release the thymine base from the nucleotide to which it was directly attached, but the thymine would still be connected to the adjacent (3’) thymine. AP endonuclease would then make a nick in this D ...
A2 5.2.3 Genetic Engineering
... • explain that genetic engineering involves the extraction of genes from one organism, or the manufacture of genes, in order to place them in another organism (often of a different species) such that the receiving organism expresses the gene product (HSW6a); • describe how sections of DNA containing ...
... • explain that genetic engineering involves the extraction of genes from one organism, or the manufacture of genes, in order to place them in another organism (often of a different species) such that the receiving organism expresses the gene product (HSW6a); • describe how sections of DNA containing ...
Section 3 - DNA Sequencing
... • ESTs are produced by purifying mRNA from cells and then using an enzyme called reverse transcriptase to convert these to copy DNA (cDNA). The DNA is then cloned in bacteria and sequenced. • The sequence obtained is usually only short (c. 700 base pairs) and may not be very accurate, but ESTs still ...
... • ESTs are produced by purifying mRNA from cells and then using an enzyme called reverse transcriptase to convert these to copy DNA (cDNA). The DNA is then cloned in bacteria and sequenced. • The sequence obtained is usually only short (c. 700 base pairs) and may not be very accurate, but ESTs still ...
Powerpoint
... Hardy-Weinberg equilibrium applies to portions of the genome that do not affect phenotype ...
... Hardy-Weinberg equilibrium applies to portions of the genome that do not affect phenotype ...
Mechanisms
... Reactions with target molecules Cellular deregulation Repair mechanisms “Essentials of Toxicology” by Klaassen Curtis D. and Watkins John B ...
... Reactions with target molecules Cellular deregulation Repair mechanisms “Essentials of Toxicology” by Klaassen Curtis D. and Watkins John B ...
dna & rna - Department of Electrical Engineering and Computing
... biological material to solve a reasonable sized problem for the “computation” to be feasible ...
... biological material to solve a reasonable sized problem for the “computation” to be feasible ...
„DNA damage“?
... Repair of double strand breaks • consequences of DSBs can be very severe (chromosome aberrations) • two repair pathways: • non-homologous end joining: conceptually simple pathway that involves the religation of broken ends (without using a homologous template • less accurate: may loss of a few nucl ...
... Repair of double strand breaks • consequences of DSBs can be very severe (chromosome aberrations) • two repair pathways: • non-homologous end joining: conceptually simple pathway that involves the religation of broken ends (without using a homologous template • less accurate: may loss of a few nucl ...
draft key
... A and C must be 30 map units (cM) apart. In a cross, they exhibit only 26% recombination due to the effect of multiple crossovers. ...
... A and C must be 30 map units (cM) apart. In a cross, they exhibit only 26% recombination due to the effect of multiple crossovers. ...
Genetic Engineering
... • Gene therapy is a technique that uses genes to treat disease • Allows doctors to treat a disorder by inserting a gene into a patient’s cells instead of using drugs or surgery – Replaces a mutated gene with a healthy copy of the gene ...
... • Gene therapy is a technique that uses genes to treat disease • Allows doctors to treat a disorder by inserting a gene into a patient’s cells instead of using drugs or surgery – Replaces a mutated gene with a healthy copy of the gene ...
DNA - The Double Helix
... alternating sugar and phosphate molecules. The sugar is Deoxyribose. Color all the phosphates pink (one is labeled with a "p"). Color all the Deoxyribose a LIGHT blue (one is labeled with a "D"). The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded lette ...
... alternating sugar and phosphate molecules. The sugar is Deoxyribose. Color all the phosphates pink (one is labeled with a "p"). Color all the Deoxyribose a LIGHT blue (one is labeled with a "D"). The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded lette ...