Slide 1
... Ex: Hold DNA out straight so that it has no Writhe, add of take out twist, then let fold up (Twist goes into Writhe). Normal DNA is negatively supercoiled, -0.06 = 6 turns for every 100 taken out. Why? Helps unwind DNA– makes it easier to uncoil, separate strands. Enzymes which do this called Topois ...
... Ex: Hold DNA out straight so that it has no Writhe, add of take out twist, then let fold up (Twist goes into Writhe). Normal DNA is negatively supercoiled, -0.06 = 6 turns for every 100 taken out. Why? Helps unwind DNA– makes it easier to uncoil, separate strands. Enzymes which do this called Topois ...
Bryan Fong - Angelfire
... plates, most of them were red with a few possibly white exceptions. The potential Laccolonies were purified on LB and then tested on back onto MacLac- agar plates. It shows that the possible Lac- colonies could utilize the sugar lactose because the new MacLac plates made all had red colonies. We did ...
... plates, most of them were red with a few possibly white exceptions. The potential Laccolonies were purified on LB and then tested on back onto MacLac- agar plates. It shows that the possible Lac- colonies could utilize the sugar lactose because the new MacLac plates made all had red colonies. We did ...
KlenTherm™ DNA Polymerase
... KlenTherm™ DNA Polymerase is thermostable polymerase corresponding to the KlenTaq Polymerase described by W. M. Barnes. It is a N-terminally truncated Taq DNA polymerase. As expressed from a gene construct in E.coli, translation initiates at Met236, bypassing the 5'-3' exonuclease domain of the DNA ...
... KlenTherm™ DNA Polymerase is thermostable polymerase corresponding to the KlenTaq Polymerase described by W. M. Barnes. It is a N-terminally truncated Taq DNA polymerase. As expressed from a gene construct in E.coli, translation initiates at Met236, bypassing the 5'-3' exonuclease domain of the DNA ...
Chap 3 Recombinant DNA Technology
... tumefaciens (農桿菌, containing Ti plasmid commonly used for gene transfer into plant cells) can be used as host cells. Many vectors may provide a second Ori so the vector can shuttle between different host organisms. Vectors that contain a single broad hostrange Ori to replace the narrow host-range Or ...
... tumefaciens (農桿菌, containing Ti plasmid commonly used for gene transfer into plant cells) can be used as host cells. Many vectors may provide a second Ori so the vector can shuttle between different host organisms. Vectors that contain a single broad hostrange Ori to replace the narrow host-range Or ...
Lecture 7
... repaired (i.e. they’re fixed) • Thus, there are at least six new base changes in each kid that were not present in either parent, but this is an underestimate as there’s more since they accumulate in the germ line stem cells as the father ages • Remember, most of these are not in genes ...
... repaired (i.e. they’re fixed) • Thus, there are at least six new base changes in each kid that were not present in either parent, but this is an underestimate as there’s more since they accumulate in the germ line stem cells as the father ages • Remember, most of these are not in genes ...
DNA Replication
... Gene is part of DNA started with promoter sequence and ended with terminator sequence which serves as a template for single RNA production One gene – one RNA – one protein ...
... Gene is part of DNA started with promoter sequence and ended with terminator sequence which serves as a template for single RNA production One gene – one RNA – one protein ...
Exam 3
... 4. The Hgh antibody will bind to the Hgh made by the colonies that contain the plasmid with the hgh gene. 5. Add a radioactive secondary antibody that binds to the primary antibody in step 4. 6. Detect the radioactive spot using autoradiography (exposing a piece of X-ray film). 7. Pick the colony th ...
... 4. The Hgh antibody will bind to the Hgh made by the colonies that contain the plasmid with the hgh gene. 5. Add a radioactive secondary antibody that binds to the primary antibody in step 4. 6. Detect the radioactive spot using autoradiography (exposing a piece of X-ray film). 7. Pick the colony th ...
Lecture 10 Types of mutations Substitutions that occur in protein
... • Human global genome NER. (a) In the damage recognition step, the XPC-hHR23B complex recognizes the damage (a pyrimidine dimer in this case), binds to it, and causes localized DNA melting. XPA also aids this process. RPA binds to the undamaged DNA strand across from the damage. (b) The DNA helicase ...
... • Human global genome NER. (a) In the damage recognition step, the XPC-hHR23B complex recognizes the damage (a pyrimidine dimer in this case), binds to it, and causes localized DNA melting. XPA also aids this process. RPA binds to the undamaged DNA strand across from the damage. (b) The DNA helicase ...
Chap3 Recombinant DNA
... Multiple cloning sites: allow the choice of different restriction enzyme (containing many restriction recognition sites) ...
... Multiple cloning sites: allow the choice of different restriction enzyme (containing many restriction recognition sites) ...
MCDB 1041 3/15/13 Working with DNA and Biotechnology Part I
... plasmid containing the recombinant DNA is incorporated into the genome of a bacterium called Agrobacteria. These bacteria naturally infect plant seeds. If the Agrobacteria are made to contain the gene ...
... plasmid containing the recombinant DNA is incorporated into the genome of a bacterium called Agrobacteria. These bacteria naturally infect plant seeds. If the Agrobacteria are made to contain the gene ...
POSSIBLE TOXICITIES OF MEDICATIONS AND MEDICATIONS USED TO AVAILABILITY
... system of stagnant water before such use. A water purifying system may be advisable for high levels of copper (over 0.1 ppm) Maintenance Phase: ■ May be more liberal than in the initial phase of treatment, based on response to therapy ■ Avoid organ meat and excessive shellfish consumption ■ Careful ...
... system of stagnant water before such use. A water purifying system may be advisable for high levels of copper (over 0.1 ppm) Maintenance Phase: ■ May be more liberal than in the initial phase of treatment, based on response to therapy ■ Avoid organ meat and excessive shellfish consumption ■ Careful ...
PROYECTO GENOMA HUMANO
... BAC to BAC - 4 These pieces are fingerprinted to give each piece a unique identification tag that determines the order of the fragments. Cutting each BAC fragment with a single enzyme and finding common sequence landmarks in overlapping fragments that determine the location of each BAC along the ch ...
... BAC to BAC - 4 These pieces are fingerprinted to give each piece a unique identification tag that determines the order of the fragments. Cutting each BAC fragment with a single enzyme and finding common sequence landmarks in overlapping fragments that determine the location of each BAC along the ch ...
Solution Key 7.013 Practice Exam 2
... These proteins can be produced from the same gene due to alternative splicing of introns i.e. if the splice donor site of Intron1 base pairs with splice acceptor site of Intron 2 you get a mature mRNA corresponding toTF-1. In comparison, if both Introns 1 & 2 are spliced out as two separate exons yo ...
... These proteins can be produced from the same gene due to alternative splicing of introns i.e. if the splice donor site of Intron1 base pairs with splice acceptor site of Intron 2 you get a mature mRNA corresponding toTF-1. In comparison, if both Introns 1 & 2 are spliced out as two separate exons yo ...
09_01.jpg
... • 5% of the genome encodes genes (1.5% is coding). • 35,000 ~ 40,000 genes with multiple splicing products per gene (build 34). • Finish at April, 2003 & single chromosome papers published one by one. • The entire human genome was finished again Oct. 2004. Build 35 assembly with 2.85 billion nucleot ...
... • 5% of the genome encodes genes (1.5% is coding). • 35,000 ~ 40,000 genes with multiple splicing products per gene (build 34). • Finish at April, 2003 & single chromosome papers published one by one. • The entire human genome was finished again Oct. 2004. Build 35 assembly with 2.85 billion nucleot ...
Fig. 20.14a
... – fragments of ancient DNA from a 40,000-yearold frozen wooly mammoth, – DNA from tiny amount of blood or semen found at the scenes of violent crimes, – DNA from single embryonic cells for rapid prenatal diagnosis of genetic disorders, – DNA of viral genes from cells infected with difficult-to-detec ...
... – fragments of ancient DNA from a 40,000-yearold frozen wooly mammoth, – DNA from tiny amount of blood or semen found at the scenes of violent crimes, – DNA from single embryonic cells for rapid prenatal diagnosis of genetic disorders, – DNA of viral genes from cells infected with difficult-to-detec ...
I. Introduction: Definitions and mutation rates
... have very serious consquences, as in the case of sickle-cell anemia, mild consequences as in the case of hemoglobin C (a different amino acid substitution in position 6 of beta-globin) or no phenotype as in the case of two known amino acid substitutions at position 7 of beta-globin. Finally, base s ...
... have very serious consquences, as in the case of sickle-cell anemia, mild consequences as in the case of hemoglobin C (a different amino acid substitution in position 6 of beta-globin) or no phenotype as in the case of two known amino acid substitutions at position 7 of beta-globin. Finally, base s ...
Transposons - iPlant Pods
... • Subtle impact on the expression of many genes • Produces stress-inducible networks (cold, salt, others?) • Generates dominant alleles Naito et al, Nature, 2009 ...
... • Subtle impact on the expression of many genes • Produces stress-inducible networks (cold, salt, others?) • Generates dominant alleles Naito et al, Nature, 2009 ...
DNA Repair and Recombination
... Holliday Junctions and the Results of Homologous Recombination • Two Holliday junctions appear during homologous recombination. They need to be resolved by cutting 2 strands and then ligating the cut ends so that the two DNA molecules can separate from each other. • The recombination of genetic mar ...
... Holliday Junctions and the Results of Homologous Recombination • Two Holliday junctions appear during homologous recombination. They need to be resolved by cutting 2 strands and then ligating the cut ends so that the two DNA molecules can separate from each other. • The recombination of genetic mar ...
Forensics of DNA
... Dr. Jeffreys found that certain regions of DNA contained ________________________________ that were repeated over and over again next to each other. He also discovered that the number of repeated sections present in a sample could differ from individual to individual. By developing a technique to ex ...
... Dr. Jeffreys found that certain regions of DNA contained ________________________________ that were repeated over and over again next to each other. He also discovered that the number of repeated sections present in a sample could differ from individual to individual. By developing a technique to ex ...
DNA Replication
... Guanine and Cytosine – These follow the rules of base-pairing: • Adenine bonds with Thymine • Guanine bonds with Cytosine ...
... Guanine and Cytosine – These follow the rules of base-pairing: • Adenine bonds with Thymine • Guanine bonds with Cytosine ...
The Central Dogma of Molecular Biology
... 1. There is a product formed. Though biochemistry is an exact science, not every PCR is successful. There is for example a possibility that the quality of the DNA is poor, that one of the primers doesn't fit, or that there is too much starting template 2. The product is of the right size It is possi ...
... 1. There is a product formed. Though biochemistry is an exact science, not every PCR is successful. There is for example a possibility that the quality of the DNA is poor, that one of the primers doesn't fit, or that there is too much starting template 2. The product is of the right size It is possi ...
7 DNA
... • How did Griffith show that the disease-causing bacteria were killed by the heat? ▫ He tried to grow them in a petri dish. If the bacteria ...
... • How did Griffith show that the disease-causing bacteria were killed by the heat? ▫ He tried to grow them in a petri dish. If the bacteria ...
An RNA-directed nuclease mediates post
... Most studies with RNAi have been done in vitro using cell-free extracts. Upon treatment with dsRNA, a nuclease known as RISC (RNAinduced silencing complex) is assembled. RISC, a multiprotein complex, is about 500 kDa. This complex degrades target mRNAs homologous to the dsRNA in a sequence-specific ...
... Most studies with RNAi have been done in vitro using cell-free extracts. Upon treatment with dsRNA, a nuclease known as RISC (RNAinduced silencing complex) is assembled. RISC, a multiprotein complex, is about 500 kDa. This complex degrades target mRNAs homologous to the dsRNA in a sequence-specific ...