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2008 exam 3 answers
2008 exam 3 answers

... repressor does. When the effector is missing, enzyme levels are also too high, indicating too much enzyme production. Therefore the job of the effector is to act as a co-repressor and allow RPX to bind to the DNA and reduce transcription. If the effector were acting as an inducer, and it were missin ...
Transitioning from custom amplicon-based parallel
Transitioning from custom amplicon-based parallel

... multiple targetable genes [1]. By this method, therapeutic genes and gene segments can be amplified, enriched and subsequently sequenced by means of multiplex-PCR for the detection of point mutations, small deletions, insertions or duplications. This method can also be used on fragmented and chemica ...
Chapter 13
Chapter 13

... However, Initiation Complex needs the help of other proteins, Activators, in order to assemble. Some Activators bind to promoter-proximal elements, others bind to enhancer elements, due to the sequence-specific DNA binding properties of their DNA binding domains. Activators' activation domains physi ...
CILJANA MUTAGENEZA I GENETSKI MARKERI U SELEKCIJI SVINJA
CILJANA MUTAGENEZA I GENETSKI MARKERI U SELEKCIJI SVINJA

... The original method using single-primer extension was inefficient due to lower yield of mutants. The resulting mixture may contain both the original unmutated template as well as the mutant strand. The mutants may also be counter-selected due to presence of mismatch repair system which favors the me ...
Chapter 2 DNA to end Multiple Choice
Chapter 2 DNA to end Multiple Choice

... A base substitution in a gene has changed a codon. Which of these consequences could result from a base substitution in a codon? I. Another amino acid will be incorporated in the protein II. A stop codon is generated III. The same protein will be synthesized A. I only B. I and II only C. I and III o ...
Comparative Genomic Hybridization
Comparative Genomic Hybridization

... – Spotting high molecular weight DNA at sufficient concentration to obtain good ratio of signal to noise in the hybridization may be difficult. ...
Structure and function of nucleases in DNA repair: shape
Structure and function of nucleases in DNA repair: shape

... many nucleases have been studied extensively. However, in some cases, it is very difficult to identify the actual functional targets of the nucleases, because of their broad substrate specificity. Nevertheless, many candidates for nucleases are available from various genome sequences, and their func ...
Sample Exam 3 answer key
Sample Exam 3 answer key

... 7. A plant was transformed using Ti plasmid which contains a kanamycin-resistance gene between the left and right borders of the T-DNA region. Two kanamycin-resistant plants were identified for further study. The plants were allowed to self, and the results were as follows: Progeny from selfing pla ...
GenomeAnnot - Nematode bioinformatics. Analysis tools and data
GenomeAnnot - Nematode bioinformatics. Analysis tools and data

... • Search/browse to find the gene or region. • Export formats: – Screen shot – FASTA seq. – Genbank file with features annotated – Feature list (Gff, tab-delimited text) – Pip (plot of sequence identity between organisms). ...
Genetic Engineering
Genetic Engineering

... People have genetically engineered their crops and livestock for years. They would try to improve the taste, size, disease resistance, and many other traits. The methods have ranged from simply picking out the best ones, to manipulating genetic material. We will look at some of the ways in which thi ...
DNA Structure and replication notes
DNA Structure and replication notes

... spread, and eventually join together, speeding up the copying of the DNA by copying many sections at once. As the enzyme unzips the DNA, the base pairs are separated and the two sides, or strands, of DNA move apart and the bases are now available to match up with new partners. ...
Endosymbiotic Evolution: Transfer of Plastid DNA to the Nucleus
Endosymbiotic Evolution: Transfer of Plastid DNA to the Nucleus

... the result of neo transfer to the nucleus. In the cross where tp7 was the male parent, 13 resistant plants were obtained from a screen of 146,000 progeny indicating a transfer frequency in the male germline of approximately 1 event for every 11,000 pollen grains. Screening of 273,000 progeny from th ...
DNA Mutation Consequences
DNA Mutation Consequences

... nucleotides. The components of the DNA nucleotide are deoxyribose (a simple sugar), a phosphate group, and a nitrogen base. There are four possible nitrogen bases in DNA—adenine (A), guanine (G), cytosine (C), and thymine (T). In DNA, the nucleotides pair using hydrogen bonds to form a double strand ...
Lab: DNA Extraction from Human Cheek Cells
Lab: DNA Extraction from Human Cheek Cells

... Individual chromosomes can be studied using microscopes, but the double helix of a chromosome is so thin that it only be detected through innovative, high-tech procedures. Chromosomal DNA from a single cell is not visible to the naked eye. However, when chromosomal DNA is extracted from multiple cel ...
Final Exam answer key
Final Exam answer key

... b. (2 pts) Are any of the mutations dominant to wild type? No 6. (2 pts) Agouti brown looks like agouti black. Explain the phenotype of agouti and why agouti brown looks like agouti black. Explain why the dominant or wild type allele of Agouti can be considered epistatic to black or brown alleles at ...
Exam #3 Review
Exam #3 Review

... encode for different amino acids. (PLEASE do not attempt to memorize all 61 codons and the amino acids for which they encode! I will provide a copy of the table in your notes.) Since there are only 20 amino acids, it must be true that more than one codon can specify the same amino acid = degeneracy. ...
Whole-Genome Chromatin IP Sequencing (ChIP-Seq)
Whole-Genome Chromatin IP Sequencing (ChIP-Seq)

... enrich for DNA sequences bound by a particular protein in living cells. However, the widespread use of this method has been limited by the lack of a sufficiently robust method to identify all of the enriched DNA sequences. The ChIP process enriches specific crosslinked DNAprotein complexes using an ...
GENETICS: BIOLOGY HSA REVIEW
GENETICS: BIOLOGY HSA REVIEW

... assembly of cars, ribosomes use mRNA to direct the assembly of proteins. The mRNA is “read” three bases at a time by the ribosome. As this happens, another type of RNA called transfer-RNA (t-RNA), moves in with an attached amino acid. The exposed nucleotides of the t-RNA (called the anticodon) provi ...
Mutations Notes
Mutations Notes

... happens when things go wrong? ...
Powerpoint template for scientific posters (Swarthmore
Powerpoint template for scientific posters (Swarthmore

... various classes of genetic mutations, including tumor suppressor genes, transcription factors/core-binding factors (CBFs), and chromosomal translocations and deletions. One specific deletion on chromosome 9, del(9q), and the translocation t(8;21), have been found together in AML, and it is likely th ...
CHAPTER 19
CHAPTER 19

... Answer: All vectors have the ability to replicate when introduced into a living cell. This ability is due to a DNA sequence known as an origin of replication, which determines the host cell specificity of a vector. Modern vectors also contain convenient restriction sites where geneticists can insert ...


... of a ladder, with hydrogen-bonded base pairs composing the rungs. This ladder is twisted into a helix . The Watson and Crick model also accurately predicted DNA replicates. As the helix unzips, each parental strand serves as the template for the synthesis of a new daughter strand. Through replicatio ...
Notes
Notes

... or egg cell, the altered gene would become part of the genetic makeup of the offspring ● the result could be:  a new trait (beneficial or harmful);  a protein that does not work correctly;  miscarriage ...
NOTES: 13.3
NOTES: 13.3

... or egg cell, the altered gene would become part of the genetic makeup of the offspring ● the result could be:  a new trait (beneficial or harmful);  a protein that does not work correctly;  miscarriage ...
What is the Structure of DNA?
What is the Structure of DNA?

... correct model. They used density labeling to distinguish parent DNA strands from new DNA strands. ...
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Zinc finger nuclease

Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms.
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