DNA
... learn how certain types of bacteria caused pneumonia. • He isolated two different strains of pneumonia bacteria from mice and grew them in his lab. ...
... learn how certain types of bacteria caused pneumonia. • He isolated two different strains of pneumonia bacteria from mice and grew them in his lab. ...
replicate, transcribe, translate
... i.e., is responsible for the replication of chromosomal DNA, plasmid DNA, phage DNA, etc. It can also correct replication errors by removing nucleotides from the 3’ ends of nucleotide strands. It is a doublestranded DNA binding protein, so requires the activity of DNA polymerase I. It forms parts of ...
... i.e., is responsible for the replication of chromosomal DNA, plasmid DNA, phage DNA, etc. It can also correct replication errors by removing nucleotides from the 3’ ends of nucleotide strands. It is a doublestranded DNA binding protein, so requires the activity of DNA polymerase I. It forms parts of ...
1. DNA SEQUENCER (Applied Biosystems, 3730xl DNA Analyzer)
... DNA Sequencer is a method of determining the order of nucleotide bases such as adenine, guanine, cytosine and thymine in a DNA oligonucleotide. It is useful in basic research studying fundamental biological processes in diagnostic or forensic research. We have the 96-capillary 3730xl DNA Analyzer as ...
... DNA Sequencer is a method of determining the order of nucleotide bases such as adenine, guanine, cytosine and thymine in a DNA oligonucleotide. It is useful in basic research studying fundamental biological processes in diagnostic or forensic research. We have the 96-capillary 3730xl DNA Analyzer as ...
Bacterial transformation - BLI-Research-Synbio-2014-session-1
... together. • When DNA from two sources is joined together, the enzyme DNA ligase is used to catalyze bonding between sugar and phosphate groups in the DNA backbone. • DNA from a “foreign” source (plant, animal, viral, bacterial, yeast) is generally bonded to vector DNA. Vectors can be bacterial plasm ...
... together. • When DNA from two sources is joined together, the enzyme DNA ligase is used to catalyze bonding between sugar and phosphate groups in the DNA backbone. • DNA from a “foreign” source (plant, animal, viral, bacterial, yeast) is generally bonded to vector DNA. Vectors can be bacterial plasm ...
DNA extraction from cheek cells protocol I mailed to you
... 6. Complete the following sentences to describe the structure of DNA. In the backbone of each strand in the DNA double helix molecule, the sugar of one nucleotide is bonded to the __________________ in the next nucleotide. The ________________ of the nucleotides in each strand of DNA extend toward e ...
... 6. Complete the following sentences to describe the structure of DNA. In the backbone of each strand in the DNA double helix molecule, the sugar of one nucleotide is bonded to the __________________ in the next nucleotide. The ________________ of the nucleotides in each strand of DNA extend toward e ...
Genetic Engineering
... Problems with PCR • Contamination: Need a clean room environment. No dust mites or bacteria • Small segments of DNA: if its too big the primer will attach to the wrong thing, an incorrect DNA sequence would result. • The nucleotide sequence must be known in order to create the correct primers. • Di ...
... Problems with PCR • Contamination: Need a clean room environment. No dust mites or bacteria • Small segments of DNA: if its too big the primer will attach to the wrong thing, an incorrect DNA sequence would result. • The nucleotide sequence must be known in order to create the correct primers. • Di ...
DNA Function in Heredity Chapter 11
... • The chromosome theory of inheritance was proposed in ~1903 – chromosomes behave much like Mendel’s “factors” • DNA was discovered in the mid-1800’s – “simple repetitive sequences of A, C, T, G” – “too simple to store complex information” ...
... • The chromosome theory of inheritance was proposed in ~1903 – chromosomes behave much like Mendel’s “factors” • DNA was discovered in the mid-1800’s – “simple repetitive sequences of A, C, T, G” – “too simple to store complex information” ...
2013 Charlotte-Mecklenburg Schools Science Elective Resource
... comprised of a sugar phosphate backbone, and nitrogenous bases paired via hydrogen bonding.) o Students will be able to explain why variations in nitrogenous base sequences aid in the distinctiveness of an individual. HS-FS- F-1b o Students will know, describe and sequence the contributions of the ...
... comprised of a sugar phosphate backbone, and nitrogenous bases paired via hydrogen bonding.) o Students will be able to explain why variations in nitrogenous base sequences aid in the distinctiveness of an individual. HS-FS- F-1b o Students will know, describe and sequence the contributions of the ...
Chapter 13 DNA - Pearson Places
... Explain why a DNA molecule is able to produce a replica of itself. A12. Because nitrogen base pairing ensures that the opposite polymer strand is produced from each half of the double helix Q13. How are DNA databases useful for forensic analysis? A13. To eliminate individual from suspicion; to ident ...
... Explain why a DNA molecule is able to produce a replica of itself. A12. Because nitrogen base pairing ensures that the opposite polymer strand is produced from each half of the double helix Q13. How are DNA databases useful for forensic analysis? A13. To eliminate individual from suspicion; to ident ...
DNA - thatscienceguy
... Adenine (A) always pairs with thymine (T) forming 2 hydrogen bonds A-T Guanine (G) always pairs with cytosine (C) forming 3 hydrogen bonds G-C ...
... Adenine (A) always pairs with thymine (T) forming 2 hydrogen bonds A-T Guanine (G) always pairs with cytosine (C) forming 3 hydrogen bonds G-C ...
Gel Electrophoresis – Virtual Lab
... represents the uniqueness of a person’s DNA. Individuals might have some of the same fragments in common, but it is very unlikely that all of them would be the same. Usually DNA fingerprinting compares at least five regions of the genome. That way it is more certain that the pattern of DNA fragments ...
... represents the uniqueness of a person’s DNA. Individuals might have some of the same fragments in common, but it is very unlikely that all of them would be the same. Usually DNA fingerprinting compares at least five regions of the genome. That way it is more certain that the pattern of DNA fragments ...
PDF file - the Houpt Lab
... Expt 2. Hershey & Chase Phage viruses are composed of DNA and protein. Is the genetic material in the protein, or in the DNA? In other words, does the phage inject the bacteria with protein or DNA? Use radioactive isotopes to label protein or label DNA. Sulfur is an element only in protein: so use 3 ...
... Expt 2. Hershey & Chase Phage viruses are composed of DNA and protein. Is the genetic material in the protein, or in the DNA? In other words, does the phage inject the bacteria with protein or DNA? Use radioactive isotopes to label protein or label DNA. Sulfur is an element only in protein: so use 3 ...
DNA 1: Today`s story, logic & goals
... • 1-base Fluorescent, isotopic or Mass-spec* primer extension (Pastinen97) • 30-base extension Pyrosequencing (Ronaghi99)* • 700-base extension, capillary arrays dideoxy* (Tabor95, Nickerson97, Heiner98) ...
... • 1-base Fluorescent, isotopic or Mass-spec* primer extension (Pastinen97) • 30-base extension Pyrosequencing (Ronaghi99)* • 700-base extension, capillary arrays dideoxy* (Tabor95, Nickerson97, Heiner98) ...
Poster
... DNA without the help of other chaperones. Histone chaperones like NAP1 are essential in cells because without them the first step in protein synthesis, transcription – the process of making RNA copies of the genes encoded in DNA – cannot occur because RNA Polymerase needs to access the DNA strands. ...
... DNA without the help of other chaperones. Histone chaperones like NAP1 are essential in cells because without them the first step in protein synthesis, transcription – the process of making RNA copies of the genes encoded in DNA – cannot occur because RNA Polymerase needs to access the DNA strands. ...
CH 16 Vocab
... A short stretch of RNA with a free 3' end, bound by complementary base pairing to the template strand, that is elongated with DNA nucleotides during DNA replication. Pyrimidine One of two types of nitrogenous bases found in nucleotides, characterized by a six-membered ring. Cytosine (C), thymine (T) ...
... A short stretch of RNA with a free 3' end, bound by complementary base pairing to the template strand, that is elongated with DNA nucleotides during DNA replication. Pyrimidine One of two types of nitrogenous bases found in nucleotides, characterized by a six-membered ring. Cytosine (C), thymine (T) ...
T4 DNA Ligase (5U/µl) - GRiSP Research Solutions
... 1 µl T4 DNA Ligase (5U/µl) (Optional: 1µl PEG 6000* (10x) [not provided]) nuclease-free water up to 10µl 2. Incubate at 22ºC for 5-15 min. 3. (Optional): heat-inactivate at 65ºC for 10min (do not perform if PEG 6000 was included!). 4. Use 5-10µl of the ligation mixture for the transformation o ...
... 1 µl T4 DNA Ligase (5U/µl) (Optional: 1µl PEG 6000* (10x) [not provided]) nuclease-free water up to 10µl 2. Incubate at 22ºC for 5-15 min. 3. (Optional): heat-inactivate at 65ºC for 10min (do not perform if PEG 6000 was included!). 4. Use 5-10µl of the ligation mixture for the transformation o ...
Lab - TeacherWeb
... Sort the DNA nucleotides into 4 separate piles according to their nitrogenous base and count them. Check the front of the envelope to be sure they are all there. Let your teacher know if you are missing any nucleotides. ...
... Sort the DNA nucleotides into 4 separate piles according to their nitrogenous base and count them. Check the front of the envelope to be sure they are all there. Let your teacher know if you are missing any nucleotides. ...
DNA Methylation studies
... levels. It involves digestion of DNA followed by radiolabelling of the newly created DNA ends and then digestion of this DNA to obtain free nucleotides. If the first cleavage had occurred next to a methylated base, this base would now be radioactive and could be identified by its different migration ...
... levels. It involves digestion of DNA followed by radiolabelling of the newly created DNA ends and then digestion of this DNA to obtain free nucleotides. If the first cleavage had occurred next to a methylated base, this base would now be radioactive and could be identified by its different migration ...
Forensic DNA Fingerprinting Kit - Bio-Rad
... Level 1 questions are simple to adapt and do not add extra days to the running of this laboratory. An example of how to organize and execute a Level 1 question is given below. Level 2 questions may add a few days onto the lab and may require some additional materials to answer. Level 3 questions are ...
... Level 1 questions are simple to adapt and do not add extra days to the running of this laboratory. An example of how to organize and execute a Level 1 question is given below. Level 2 questions may add a few days onto the lab and may require some additional materials to answer. Level 3 questions are ...
chapter11
... 4. DNA synthesis always proceeds in a 5’ 3’ direction. 5. DNA polymerases catalyze the linking together of the nucleotide subunits. 6. Nucleotides with three phosphate groups are used as substrates for the polymerization reaction. Two of the phosphates are removed and the nucleotide is added to the ...
... 4. DNA synthesis always proceeds in a 5’ 3’ direction. 5. DNA polymerases catalyze the linking together of the nucleotide subunits. 6. Nucleotides with three phosphate groups are used as substrates for the polymerization reaction. Two of the phosphates are removed and the nucleotide is added to the ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.