Divergent roles for the two PolI-like organelle DNA polymerases of
... DNA Double-Strand Breaks DNA-synthesis activity is required for the repair of DNA damages by mechanisms such as homologous recombination (HR). To test for a role in DNA repair, the PolI mutants were treated with ciprofloxacin, a molecule that induces DNA double-strand breaks (DSBs) specifically in p ...
... DNA Double-Strand Breaks DNA-synthesis activity is required for the repair of DNA damages by mechanisms such as homologous recombination (HR). To test for a role in DNA repair, the PolI mutants were treated with ciprofloxacin, a molecule that induces DNA double-strand breaks (DSBs) specifically in p ...
Bio II Ch 16 Molecular Basis of Inheritance
... elongation of a DNA strand in the 5' 3' direction. The function of DNA polymerase is to add nucleotides to the end of a growing DNA strand and proof-read • The strands that make up DNA are antiparallel. This means that the 5' to 3' direction of one strand runs counter to the 5' to 3' direction of th ...
... elongation of a DNA strand in the 5' 3' direction. The function of DNA polymerase is to add nucleotides to the end of a growing DNA strand and proof-read • The strands that make up DNA are antiparallel. This means that the 5' to 3' direction of one strand runs counter to the 5' to 3' direction of th ...
Development of a qPCR Method to Measure Mitochondrial
... Abstract: DNA damage quantitation assays such as the comet assay have focused on the measurement of total nuclear damage per cell. The adoption of PCR-based techniques to quantify DNA damage has enabled sequence- and organelle-specific assessment of DNA lesions. Here we report on an adaptation of a ...
... Abstract: DNA damage quantitation assays such as the comet assay have focused on the measurement of total nuclear damage per cell. The adoption of PCR-based techniques to quantify DNA damage has enabled sequence- and organelle-specific assessment of DNA lesions. Here we report on an adaptation of a ...
DNA Polymerase I
... • New DNA chains are initiated by short RNA primers synthesized by DNA primase. • DNA synthesis is catalyzed by enzymes called DNA polymerases. • All DNA polymerases require a primer strand, which is extended, and a template strand, which is copied. ...
... • New DNA chains are initiated by short RNA primers synthesized by DNA primase. • DNA synthesis is catalyzed by enzymes called DNA polymerases. • All DNA polymerases require a primer strand, which is extended, and a template strand, which is copied. ...
Document
... Molecular analyses necessary Take advantage of enzymes and reactions that naturally occur in bacteria ...
... Molecular analyses necessary Take advantage of enzymes and reactions that naturally occur in bacteria ...
Biochemical and functional characterization of Plasmodium
... fidelity of DNA synthesis required for accurate genome replication [6]. Pol δ holoenzyme participates in replicative synthesis in concert with the processivity factor proliferating cell nuclear antigen (PCNA). Kinetic and binding studies have shown that PCNA increases Pol δ processivity as well as a ...
... fidelity of DNA synthesis required for accurate genome replication [6]. Pol δ holoenzyme participates in replicative synthesis in concert with the processivity factor proliferating cell nuclear antigen (PCNA). Kinetic and binding studies have shown that PCNA increases Pol δ processivity as well as a ...
A novel type of replicative enzyme harbouring ATPase, primase and
... Fig. 1. Hypothetical domain organization and puri®cation of ORF904. (A) Two domains can be identi®ed by sequence comparison: an N-terminal domain, presumably carrying the primase and DNA polymerase activity; and a C-terminal helicase domain. The N-terminal domain was tentatively named prim/pol domai ...
... Fig. 1. Hypothetical domain organization and puri®cation of ORF904. (A) Two domains can be identi®ed by sequence comparison: an N-terminal domain, presumably carrying the primase and DNA polymerase activity; and a C-terminal helicase domain. The N-terminal domain was tentatively named prim/pol domai ...
DNA
... of DNA that are not expressed as polypeptides. This non-coding DNA is still important to organisms for a variety of reasons. ...
... of DNA that are not expressed as polypeptides. This non-coding DNA is still important to organisms for a variety of reasons. ...
myDNA
... DNA letters are strung together in chains, and are attached along the DNA backbone. Two DNA strands wrap around each other in a spiral shape called a double helix. Each chromosome contains one pair of extremely long DNA strands, twisted in a double helix. Two strands = Double helix ...
... DNA letters are strung together in chains, and are attached along the DNA backbone. Two DNA strands wrap around each other in a spiral shape called a double helix. Each chromosome contains one pair of extremely long DNA strands, twisted in a double helix. Two strands = Double helix ...
Globicatella sulfidifaciens sp. nov., isolated from purulent infections
... * This table is based on the results of Collins et al. (1999) and our results obtained with the four collection strains used in this study for comparative purposes. ...
... * This table is based on the results of Collins et al. (1999) and our results obtained with the four collection strains used in this study for comparative purposes. ...
DNA recognition code of transcription factors
... side of a Watson-Crick base pair, a protein side chain can bind to a particular DNA base pair through a bidentate hydrogen bond, thereby discriminating between the DNA base pairs. They modeled two specific amino acid-nucleotide base interactions, Arg-G and Asn/Gln-A, which were later found in many c ...
... side of a Watson-Crick base pair, a protein side chain can bind to a particular DNA base pair through a bidentate hydrogen bond, thereby discriminating between the DNA base pairs. They modeled two specific amino acid-nucleotide base interactions, Arg-G and Asn/Gln-A, which were later found in many c ...
Molecular Inheritance
... Your answer: nitrogen base, five-carbon sugar, and a phosphate group Correct. Each nucleotide unit of DNA consists of a nitrogenous base (A, G, C, or T), the sugar deoxyribose, and a phosphate group. ...
... Your answer: nitrogen base, five-carbon sugar, and a phosphate group Correct. Each nucleotide unit of DNA consists of a nitrogenous base (A, G, C, or T), the sugar deoxyribose, and a phosphate group. ...
The Influence of Hydrogen Donors on Breakage of Parental DNA
... either. Similar experi ondary breaks due to energy or damage transfer ments were then performed with normal and bi is just a few base pairs away from the primary lesion in the complementary strand. Hydrogen do filarly labeled DNA and 254 nm radiation. Again no protection was observed. These resul ...
... either. Similar experi ondary breaks due to energy or damage transfer ments were then performed with normal and bi is just a few base pairs away from the primary lesion in the complementary strand. Hydrogen do filarly labeled DNA and 254 nm radiation. Again no protection was observed. These resul ...
DNA structurereplication2014
... A new strand is formed by pairing complementary bases with the old strand. Two molecules are made. Each has one new and one old DNA strand. ...
... A new strand is formed by pairing complementary bases with the old strand. Two molecules are made. Each has one new and one old DNA strand. ...
Interaction of Rat Testis Protein, TP, with Nucleic Acids in Vitro
... and a rather interestingprotein in that it is a smallbut highly basic protein made up of 54 amino acids (6). It contains19% lysine and 21% arginine, thus falling in between the histones on the one hand and protein S1 (60% arginine) on the other. ...
... and a rather interestingprotein in that it is a smallbut highly basic protein made up of 54 amino acids (6). It contains19% lysine and 21% arginine, thus falling in between the histones on the one hand and protein S1 (60% arginine) on the other. ...
Electroosmotic screening of the DNA charge in a
... time of water. This indicates that ions are not bound to DNA. The chloride ions in the same region are depleted. Up to 30 Å away from the DNA central axis, the density of potassium ions is higher than that of chloride ions. Under the action of the external electric field, ions of both types move in ...
... time of water. This indicates that ions are not bound to DNA. The chloride ions in the same region are depleted. Up to 30 Å away from the DNA central axis, the density of potassium ions is higher than that of chloride ions. Under the action of the external electric field, ions of both types move in ...
Isolation, Cloning, and Sequencing of the Salmonella typhimurium dd1A Gene with Purification and Characterization of its Product, D-Alanine:D-Alanine Ligase (ADP Forming).
... Figure 1). The temperature-sensitive mutation in ST640, however, was never backcrossed into a nonmutagenized background. Thus, it has not been shown whether or not ddl is an essential structural gene for cell wall synthesis and cell viability in E. coli. The goal of this work, therefore, was 2-fold: ...
... Figure 1). The temperature-sensitive mutation in ST640, however, was never backcrossed into a nonmutagenized background. Thus, it has not been shown whether or not ddl is an essential structural gene for cell wall synthesis and cell viability in E. coli. The goal of this work, therefore, was 2-fold: ...
Using DNA to solve the Bounded Post Correspondence Problem
... nucleotides are linked together end-to-end to form DNA strands. A short single-stranded polynucleotide chain, usually less than 30 nucleotides long, is called an oligonucleotide. The DNA sequence has a polarity: a sequence of DNA is distinct from its reverse. The two distinct ends of a DNA sequence ...
... nucleotides are linked together end-to-end to form DNA strands. A short single-stranded polynucleotide chain, usually less than 30 nucleotides long, is called an oligonucleotide. The DNA sequence has a polarity: a sequence of DNA is distinct from its reverse. The two distinct ends of a DNA sequence ...
Variable effects of DNA-synthesis inhibitors upon DNA methylation
... intracellularly trapped [ H]-dCTP (21), this result may have been due to incorporation from this pool immediately after aphidicolin was removed. Thus the methylation level determined after a 24 h chase would represent the average value of the pre-existing hypermethylated sequences and the newly inco ...
... intracellularly trapped [ H]-dCTP (21), this result may have been due to incorporation from this pool immediately after aphidicolin was removed. Thus the methylation level determined after a 24 h chase would represent the average value of the pre-existing hypermethylated sequences and the newly inco ...
NUCLEIC ACIDS
... Four core histones: H2a, H2b, H3 and H4 an one linker histomes: H1 In many eukaryotes there are amino acid sequence variants of all the histones except H4. All histones are post-translationally modified at various stages of cell cycle. ...
... Four core histones: H2a, H2b, H3 and H4 an one linker histomes: H1 In many eukaryotes there are amino acid sequence variants of all the histones except H4. All histones are post-translationally modified at various stages of cell cycle. ...
Molecular Structure of the GARP Family of Plant Myb
... Figure 5A. The purified ARR10-B displayed an ability to associate efficiently with AGATT and to associate slightly less efficiently with GGATT, CGATT, and TGATT, whereas GcATT, GGtTT, GGAaT, and GGATg were unfavorable. The apparent enhancement in binding affinity observed for GGATC, compared with GG ...
... Figure 5A. The purified ARR10-B displayed an ability to associate efficiently with AGATT and to associate slightly less efficiently with GGATT, CGATT, and TGATT, whereas GcATT, GGtTT, GGAaT, and GGATg were unfavorable. The apparent enhancement in binding affinity observed for GGATC, compared with GG ...
dna and it`s role in heredity
... your fingers would represent the sugar-phosphate backbone. Only one type of DNA, called Z-DNA, is left-handed. • The DNA double helix is anti-parallel, which means that the 5' end of one strand is paired with the 3' end of its complementary strand (and vice versa). As shown in Figure 4, nucleotides ...
... your fingers would represent the sugar-phosphate backbone. Only one type of DNA, called Z-DNA, is left-handed. • The DNA double helix is anti-parallel, which means that the 5' end of one strand is paired with the 3' end of its complementary strand (and vice versa). As shown in Figure 4, nucleotides ...
File
... A) the leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction. B) the leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized b ...
... A) the leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction. B) the leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized b ...