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Bio 9B: Tuesday, 2.1.11Title: DNA Structure & Function
Bio 9B: Tuesday, 2.1.11Title: DNA Structure & Function

... Genetics: the study of heredity  What determines an organism’s traits (characteristics)?  How are traits passed on from one cell to another, and from parents to offspring? DNA: the molecule that carries genetic information DNA has two important functions:  Replicate – make exact copies of itself ...
Recombinant DNA Technology I Restriction Enzymes
Recombinant DNA Technology I Restriction Enzymes

... corner. The Home icon button will automatically set the slide back to the key questions which we are trying to answer with this course material. Several pages have hyperlinks which navigate immediately to either specific slides OR navigate away from this module via the default web browser. To return ...
DNA is the genetic material
DNA is the genetic material

... Hershey/Chase Experiments• They grew one batch of T2 phage in the presence of radioactive sulfur, marking the proteins (but not the DNA) radioactive. • They grew another batch in the presence of radioactive phosphorus, marking the DNA (but not the proteins) radioactive. • They allowed each batch to ...
Word file (68 KB )
Word file (68 KB )

... used these conditions to scan the second and third chromosomes for regions capable of suppressing the temperature sensitive lethality of cdk7ts1 mutants. The screen was carried out by crossing cdk7ts1 flies to a collection of 144 deficiency strains that are heterozygous for a deletion of the second ...
10 Modeling DNA Structure
10 Modeling DNA Structure

... end, while the other end is the 3’ end. In a double-stranded DNA molecule, the 5’ end of one strand is opposite the 3’ end of the other strand. Thus, the two strands of DNA in a double helix are said to be antiparallel. The Work of Erwin Chargaff In the late 1940s, Erwin Chargaff, an Austrian bioche ...
Communication: Formation of Knots in Partially Replicated DNA
Communication: Formation of Knots in Partially Replicated DNA

... (Bliska & Cozzarelli, 1987). Further treatments of the DNA-like deproteinisation and subsequent RecA covering do not change the type of knots formed, and thus we can use this topological information to draw conclusions about the structural arrangement of partially replicated DNA molecules in vivo. A ...
Gene organization, transcription signals and processing of the
Gene organization, transcription signals and processing of the

... Preparation of cells, cellular RNA and DNA: cloning and DNA sequencing Cells from T. tenax were kindly provided by W. Zillig and grown under the conditions described by Zillig et al. [18]. Total cellular RNA was prepared using hot phenol extractions (65°C) essentially as described by Aiba etal. [19] ...
The Influence of Hydrogen Donors on Breakage of Parental DNA
The Influence of Hydrogen Donors on Breakage of Parental DNA

... strand breaks in parental thymine containing DNA. of up to 100). Here the protection factor is about 20 —25. If this Bacterial transformation provides the opportuni­ secondary damage is due to the transfer of excita­ ty for testing the effect of cysteamine and other tion energy one would expect that ...
RNA vs. DNA
RNA vs. DNA

... • The results of Griffith showed that the bacteria treated with ...
Molecular Basis of Heredity
Molecular Basis of Heredity

... million base pairs in its single chromosome and divide to form two identical daughter cells. • A human cell can copy its 6 billion base pairs and divide into daughter cells in only a few hours. • This process is remarkably accurate, with only one error per billion nucleotides. • More than a dozen en ...
jeopardy practice
jeopardy practice

... • The results of Griffith showed that the bacteria treated with ...
Therapeutic Targeting of the DNA Mismatch Repair Pathway
Therapeutic Targeting of the DNA Mismatch Repair Pathway

... Fig. 2. A, schematic of mammalian MMR as a postreplicative repair process. Mismatches (X) caused by errors in replication are shown in the daughter strands of replicated DNA (mismatches are shown on both the leading and lagging strands of replicated DNA). MMR involves (i) detection of the mismatch a ...
Proceedings Template - WORD
Proceedings Template - WORD

... anti-parallel fashion to form a double strand. This double strand resembles the shape of a twisted ladder or a double helix (Figure 2). The process of combining two single strands into a double strand is known as ‘Annealing’. Similarly a process known as ‘Denaturing’ allows the double helix to separ ...
Selecting for Mutant Reverse Transcriptases
Selecting for Mutant Reverse Transcriptases

... Summary: Combating HIV and other retroviruses is complicated by high rates of genetic variation, which leads to the rapid evolution of drug resistant strains. Genetic variation stems from mutations that arise when viral reverse transcriptase (RT) introduces base pair mismatches during conversion of ...
dna and it`s role in heredity
dna and it`s role in heredity

... •  Most DNA double helices are right-handed; that is, if you were to hold your right hand out, with your thumb pointed up and your fingers curled around your thumb, your thumb would represent the axis of the helix and your fingers would represent the sugar-phosphate backbone. Only one type of DNA, c ...
A reagentless biosensor for mRNA: a new tool to study transcription
A reagentless biosensor for mRNA: a new tool to study transcription

... A fluorescently labelled single stranded binding protein (SSB) from Escherichia coli has been successfully used as a single stranded DNA (ssDNA) biosensor for monitoring helicase activity (19-23). E. coli SSB is a well characterised homo-tetrameric protein containing 4 OB-fold domains (24). The form ...
biomolecules (introduction, structure and functions)
biomolecules (introduction, structure and functions)

... difference between DNA and RNA is the type of sugar they contain. RNA contains the sugar D-ribose (hence called ribonucleic acid, RNA) whereas DNA contains its derivatives 2’-deoxy-D-ribose, where the 2’-hydroxyl group of ribose is replaced by hydrogen (hence called deoxyribonucleic acid, DNA). Suga ...
Chapter 16 The Molecular Basis of Inheritance Multiple
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... A) adding a single 5ʹ cap structure that resists degradation by nucleases B) causing specific double strand DNA breaks that result in blunt ends on both strands C) causing linear ends of the newly replicated DNA to circularize D) adding numerous short DNA sequences such as TTAGGG, which form a hairp ...
Single-base Mutation
Single-base Mutation

... in¯uenza A virus and the Moloney murine sarcoma virus to be of the order of 10 2 substitution mutations per nucleotide site per year, that is, approximately 2 million times higher than the rate of mutation in the nuclear DNA of vertebrates. The rate of mutation in the Rous sarcoma virus may be even ...
Document
Document

... What is multiplexing and why is it used in DNA profiling? What characteristics are revealed by the amelogenin gene and Y-STRs? What are the three main differences between nuclear DNA and mitochondrial DNA? Name two advantages and two disadvantages of mitochondrial DNA analysis compared to nuclear DN ...
Nucleic Acids exploringorigins.org - vtu-nptel
Nucleic Acids exploringorigins.org - vtu-nptel

... b) conservative c) dispersive d) semidiscontinuous 7. The sugar in RNA and DNA respectively are: a) deoxyribose, ribose b) ribose, deoxyribose c) ribose, phosphate d) ribose, uracil 8. In a nucleoside the base is bonded to sugar by a) covalently bonded to a sugar b) ionically bonded to a sugar c) hy ...
Chapter 7: Essentials of Molecular Biology Question: Which strand
Chapter 7: Essentials of Molecular Biology Question: Which strand

... leading strand, ligase, DNA polymerase lagging strand, primer, helicase single-strand binding protein, replication fork, promoter DNA polymerase III, DNA polymerase I, ligase ...
The Regulatory Region of the Larabinose Operon: Its Isolation on a
The Regulatory Region of the Larabinose Operon: Its Isolation on a

... electron microscope shows a size distribution with over 9OyA of the molecules in a symmetrical peak centered about a mean of 270 nm with a standard deviation of 25 nm (Fig. 2(a)). The standard deviation associated with our measurements of DNA length with the electron microscope generally follows the ...
DNA - Armstrong State University
DNA - Armstrong State University

... The smaller DNA fragments will move at a faster rate on the gel plate than the larger ones. The fragments are then transferred to a nylon membrane in a process called Southern blotting. To visualize the RFLPs, RFLPs, the nylon sheet is treated with radioactive probes containing a base sequence compl ...
Active-site Determination of a Pyrimidine Dimer
Active-site Determination of a Pyrimidine Dimer

... that seen for the cis-syn CPD; however, the trapping appears to be more ef®cient for both of these enzymes on the AP site-containing DNA. However, the T2P cv-pdg (lane 9) mutant appeared to be the most readily formed enzyme substrate complex on AP site-containing DNA. ...
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Helicase



Helicases are a class of enzymes vital to all living organisms. Their main function is to unpackage an organism's genes. They are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands (i.e., DNA, RNA, or RNA-DNA hybrid) using energy derived from ATP hydrolysis. There are many helicases resulting from the great variety of processes in which strand separation must be catalyzed. Approximately 1% of eukaryotic genes code for helicases. The human genome codes for 95 non-redundant helicases: 64 RNA helicases and 31 DNA helicases. Many cellular processes, such as DNA replication, transcription, translation, recombination, DNA repair, and ribosome biogenesis involve the separation of nucleic acid strands that necessitates the use of helicases.
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