The identification of circular extrachromosomal DNA in the nuclear
... sequences, could have provided the necessary components for replication and segregation. Integration into a chromosome by recombination also renders extrachromosomal DNA stable in Leishmania (Beverley et al., 1984). Alternatively, a process of neo-centromerisation, described for yeast and mammalian ...
... sequences, could have provided the necessary components for replication and segregation. Integration into a chromosome by recombination also renders extrachromosomal DNA stable in Leishmania (Beverley et al., 1984). Alternatively, a process of neo-centromerisation, described for yeast and mammalian ...
Effects of Florida Red Tides on histone variant
... dinoflagellate Karenia brevis and associated brevetoxins) are particularly detrimental in the southeastern U.S., causing high mortality rates and annual losses in excess of $40 million. The ability of marine organisms to cope with environmental stressors (including those produced during HABs) is influ ...
... dinoflagellate Karenia brevis and associated brevetoxins) are particularly detrimental in the southeastern U.S., causing high mortality rates and annual losses in excess of $40 million. The ability of marine organisms to cope with environmental stressors (including those produced during HABs) is influ ...
Studies on Chlamydomonas Chloroplast Transformation: Foreign
... ulation would be facilitated greatly by the development of a transformation-proficient in vivo expression system to permit rigorous examination of the molecular events that modulate gene expression in chloroplasts. Recently, Sanford and co-workers (Klein et al., 1987) have developed a DNA transfer t ...
... ulation would be facilitated greatly by the development of a transformation-proficient in vivo expression system to permit rigorous examination of the molecular events that modulate gene expression in chloroplasts. Recently, Sanford and co-workers (Klein et al., 1987) have developed a DNA transfer t ...
Apoptin protein multimers form distinct higher
... DNA. Furthermore, we show a single apoptin multimer to have eight independent, non-speci®c DNAbinding sites which preferentially bind strand ends, but which can also collaborate to bind longer stretches of DNA. Apoptin's high af®nity for naked, undecorated double- and single-stranded DNA and for DNA ...
... DNA. Furthermore, we show a single apoptin multimer to have eight independent, non-speci®c DNAbinding sites which preferentially bind strand ends, but which can also collaborate to bind longer stretches of DNA. Apoptin's high af®nity for naked, undecorated double- and single-stranded DNA and for DNA ...
chapter 20 notes
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
C. elegans MCM-4 is a general DNA replication and
... ortholog chk-1 contributes to different cell cycle timing of early blastomeres (Brauchle et al., 2003). Thus, DNA replication and replication checkpoint control have developmental functions that go beyond the duplication of individual cells. Studies of single-cell eukaryotes, Xenopus egg extracts an ...
... ortholog chk-1 contributes to different cell cycle timing of early blastomeres (Brauchle et al., 2003). Thus, DNA replication and replication checkpoint control have developmental functions that go beyond the duplication of individual cells. Studies of single-cell eukaryotes, Xenopus egg extracts an ...
DNA Sequencing
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
PDF - 167.13 kbytes - Istituto Superiore di Sanità
... The LAMP is a molecular biology technique that allows the amplification of specific nucleic acid fragments, which initial and terminal nucleotide sequences are known (oligonucleotide pair). If a species has its own characteristic DNA portion, due to its composition, it is possible to design 2 or 3 p ...
... The LAMP is a molecular biology technique that allows the amplification of specific nucleic acid fragments, which initial and terminal nucleotide sequences are known (oligonucleotide pair). If a species has its own characteristic DNA portion, due to its composition, it is possible to design 2 or 3 p ...
UltraClean 15 DNA Purification Kit
... Kit reagents and components should be stored at room temperature (15-30°C). DO NOT REFRIGERATE ULTRA MELT or crystals may form. If salt crystals form, heat to dissolve before using. Heating will not cause a loss of efficiency. NOTE: ULTRA SALT contains sodium iodide which may oxidize and turn yellow ...
... Kit reagents and components should be stored at room temperature (15-30°C). DO NOT REFRIGERATE ULTRA MELT or crystals may form. If salt crystals form, heat to dissolve before using. Heating will not cause a loss of efficiency. NOTE: ULTRA SALT contains sodium iodide which may oxidize and turn yellow ...
Characterization of two rice DNA methyltransferases
... MET1 MTases include a long N-terminal domain (that does not exist in prokaryotic DNA MTases) and a shorter C-terminal domain that contains some or all of ten motifs (I to X) that are well conserved among prokaryotes and eukaryotes (Lauster et al. 1989;Posfai et al. 1989;Sankpal and Rao 2002) . The p ...
... MET1 MTases include a long N-terminal domain (that does not exist in prokaryotic DNA MTases) and a shorter C-terminal domain that contains some or all of ten motifs (I to X) that are well conserved among prokaryotes and eukaryotes (Lauster et al. 1989;Posfai et al. 1989;Sankpal and Rao 2002) . The p ...
Comparison of methods for high quantity and quality - Funpec-RP
... Due to increasing concern for food safety, cow’s milk can be used for population screening for disease diagnosing, eradicating genetic diseases and disease-resistance/susceptibility studies (Psifidi et al., 2010). In early studies, the phenol-chloroform method was generally used for DNA extraction f ...
... Due to increasing concern for food safety, cow’s milk can be used for population screening for disease diagnosing, eradicating genetic diseases and disease-resistance/susceptibility studies (Psifidi et al., 2010). In early studies, the phenol-chloroform method was generally used for DNA extraction f ...
from Saccharomyces cerevisiae
... The 289 base pair Rsa I-Alu I fragment containing CEN3 DNA was isolated as described by Bloom et al. (1984). The fragment was incubated with synthetic oligonucleotide linkers encoding the Barn HI restriction recognition site in the presence of T4 DNA ligase. Enzymes were purchased from New England B ...
... The 289 base pair Rsa I-Alu I fragment containing CEN3 DNA was isolated as described by Bloom et al. (1984). The fragment was incubated with synthetic oligonucleotide linkers encoding the Barn HI restriction recognition site in the presence of T4 DNA ligase. Enzymes were purchased from New England B ...
DNA crosslinking and biological activity of a
... the fraction of double-stranded DNA that was resistant to denaturation (Fig. 2A, lanes 5±7). Crosslinks could be detected at agent concentrations as low as 0.1 mM, and at 1 mM 1-Chl ~50% of the plasmid was crosslinked and migrated as double-stranded DNA (Fig. 2A, chart). Under similar conditions, no ...
... the fraction of double-stranded DNA that was resistant to denaturation (Fig. 2A, lanes 5±7). Crosslinks could be detected at agent concentrations as low as 0.1 mM, and at 1 mM 1-Chl ~50% of the plasmid was crosslinked and migrated as double-stranded DNA (Fig. 2A, chart). Under similar conditions, no ...
Sheared DNA fragment sizing: comparison of techniques
... sperslon of repeated and nonrepeated sequences of DNA in eucaryotes ' ' In general, however, on kinetic and other grounds it is advantageous to use short DNA fragments less than 500 base pairs in length . This length not only permits separation of the repetitive and nonrepetitive components of most ...
... sperslon of repeated and nonrepeated sequences of DNA in eucaryotes ' ' In general, however, on kinetic and other grounds it is advantageous to use short DNA fragments less than 500 base pairs in length . This length not only permits separation of the repetitive and nonrepetitive components of most ...
Structure of B-DNA with Cations Tethered in the Major Groove†
... has been covalently modified by the tethering of four cationic charges. This modified version of the DDD, called here the DDD4+, is composed of [d(CGCGAAXXCGCG)]2, where X is effectively a thymine residue linked at the 5 position to an n-propyl-amine. The structure was determined from crystals soake ...
... has been covalently modified by the tethering of four cationic charges. This modified version of the DDD, called here the DDD4+, is composed of [d(CGCGAAXXCGCG)]2, where X is effectively a thymine residue linked at the 5 position to an n-propyl-amine. The structure was determined from crystals soake ...
1 X chromosome crossover formation and genome stability in
... fidelity propagation of unaltered genomic information across generations. Yet, germ cells in most metazoans also intentionally create double-strand breaks (DSBs) to promote DNA exchange between parental chromosomes, a process known as crossing over. Homologous recombination is employed in the repair ...
... fidelity propagation of unaltered genomic information across generations. Yet, germ cells in most metazoans also intentionally create double-strand breaks (DSBs) to promote DNA exchange between parental chromosomes, a process known as crossing over. Homologous recombination is employed in the repair ...
Chapter 13 Unintended Horizontal Transfer of Recombinant DNA
... DNA is continually released from living organisms (e.g. crop plants) shedding tissues or cells or from their decaying debris. The release of DNA is therefore not specific to GMOs and the effect thereof should be seen in the context of DNA released from other organisms present in the same natural sys ...
... DNA is continually released from living organisms (e.g. crop plants) shedding tissues or cells or from their decaying debris. The release of DNA is therefore not specific to GMOs and the effect thereof should be seen in the context of DNA released from other organisms present in the same natural sys ...
File
... Watson and Crick discovered that hydrogen bonds can form only between certain base pairs—adenine and thymine, and guanine and cytosine. This principle is called base pairing. ...
... Watson and Crick discovered that hydrogen bonds can form only between certain base pairs—adenine and thymine, and guanine and cytosine. This principle is called base pairing. ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.