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DNA and RNA
DNA and RNA

... cytosine, and thymine. Took samples of DNA of different cells and found that the amount of adenine was almost equal to the amount of thymine. ...
code sequence practice
code sequence practice

... Transcription – making mRNA from DNA 2. If this is your original DNA strand, what is the mRNA sequence that is synthesized? DNA Strand: C A G T G C A T T mRNA strand: 3. Now go backwards, if you are given the following mRNA strand, write the DNA strand that goes with it. mRNA strand: U C G A C C G A ...
Sliding
Sliding

... strand may remain nicked for a short time, also old strands may have chemical modifications, such as methylation. ...
Recombinant DNA Technology (b)
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... Recombinant DNA Technology Production of a unique DNA molecule by joining together two or more DNA fragments not normally associated with each other, which can replicate in the living cell. Recombinant DNA is also called Chimeric DNA Developed by Boyer and Cohen in 1973 3 different methods of D ...
from dna to protein - Aurora City Schools
from dna to protein - Aurora City Schools

... A. Friedrich Miescher – extracted DNA from the nucleus of white blood cells. ...
DNA Technology and its Applications
DNA Technology and its Applications

... Using the technology of recombinant DNA, we are able to introduce specific genes from one organism into another. A transgenic organism is an organism that has been genetically engineered to contain 1 or more genes ...
Genetic Engineering - Biology Class With Mrs. Caskey
Genetic Engineering - Biology Class With Mrs. Caskey

... List one behavior or physical characteristic of that species that was targeted through selective breeding. ...
Topic 7.1
Topic 7.1

DNA Technology
DNA Technology

... Bacteria make the viral protein: Protein used as vaccine for hepatitis B Step 2. Purified DNA fused with other pieces of DNA to form recombinant DNA molecules: Fragmented donor DNA attached to a vector: Carrier of foreign genes: ...
Recall that the nucleus is a small spherical, dense body in a cell
Recall that the nucleus is a small spherical, dense body in a cell

... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
Geneticky modifikované organismy (GMO)
Geneticky modifikované organismy (GMO)

... Homologous recombination 1. extrachromosomal recombination - between two introduced molecules - frequency in plants: 1 - 4 % 2. Intrachromosomal recombination - between two loci in the same chromosome - frequency in plants: 10-5 až 10-6 ...
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... Okazaki fragment - Small pieces of DNA which form during DNA replication since DNA polymerase must work backwards on some strands. The Okazaki fragments are joined together by the enzyme DNA ligase. point mutation - A mutation which occurs when one base in the DNA molecule is replaced by another. Th ...
Random Priming - ltcconline.net
Random Priming - ltcconline.net

... Library Construction • Genomic l Library – Isolate Genomic DNA – Limited Digest with restriction enzyme – Analyze digest with gel electrophoresis – Prepare l arms – Ligate digest to arms – In vitro package l – Infect and plate ...
DNA Review Questions (answers)
DNA Review Questions (answers)

... Phosphorus was chosen because it is found in DNA, not proteins. Sulfur was chosen because it is found in protein, not DNA. 6. What did the x-ray crystallography work from Franklin tell Watson and Crick about the shape of DNA? It was a double helix with a consistent 2 nm width. 7. What is the basic s ...
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Manipulating and Analyzing DNA
Manipulating and Analyzing DNA

... recombinant DNA. You will use two different websites to understand both topics. By the end of today you should be able answer the flooring questions: What are restriction enzymes? How and why are they used in biotechnology? How do restriction enzymes play a role in recombinant DNA? Restriction Enzym ...
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Microbial Genetics - University of Montana
Microbial Genetics - University of Montana

... – Bacterial chromosomal DNA packaged into phage heads – After lysis, phage particles inject this DNA into new host – Homologous recombination: donor DNA incorporated into recipient genome • DNA replacement ...
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... Limitation to fingerprinting system: single base pair changes only detected= Surprise- only 10% of spontaneous changes are base pair changes! OH Frameshifts- one incredible hotspot- ca 2/3 of all mutations ...
DNA - The Double Helix
DNA - The Double Helix

... controls the production of proteins within the cell. These proteins in turn, form the structural units of cells and control all chemical processes within the cell. Think of proteins as the building blocks for an organism, proteins make up your skin, your hair, parts of individual cells. How you look ...
PCR
PCR

... ■ dNTPS (deoxynucleotide triphosphates) ● nucleotides with triphosphate groups ● building blocks that DNA polymerase uses to synthesize new strand ■ Buffer Solution ● provides best chem environment for best activity and stability of DNA polymerase ■ Thermal Cycler→ heats and cools tubes (with the DN ...
Recall that the nucleus is a small spherical, dense body in a cell
Recall that the nucleus is a small spherical, dense body in a cell

... Recall that the nucleus is a small spherical, dense body in a cell. It is often called the "control center" because it controls all the activities of the cell including cell reproduction, and heredity. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribon ...
CS262 Discussion Section 4
CS262 Discussion Section 4

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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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