DNATechnology
... More than 95 percent of all DNA, was called "Junk DNA" by molecular biologists, because they were unable to ascribe any function to it. However that category is becoming smaller as scientists identify non-coding DNA as introns, transposable elements, pseudogenes, VNTRs (variable number tandem repeat ...
... More than 95 percent of all DNA, was called "Junk DNA" by molecular biologists, because they were unable to ascribe any function to it. However that category is becoming smaller as scientists identify non-coding DNA as introns, transposable elements, pseudogenes, VNTRs (variable number tandem repeat ...
Recombinant DNA technology engineering) involves combining genes from genes.
... the late 1960s. •In nature, bacteria use restriction enzymes to cut up intruder DNA from phages and from other organisms into nonfunctional pieces. The bacteria first chemically modify their own DNA so that it will not be cut. •Several hundred different restriction enzymes have been discovered that ...
... the late 1960s. •In nature, bacteria use restriction enzymes to cut up intruder DNA from phages and from other organisms into nonfunctional pieces. The bacteria first chemically modify their own DNA so that it will not be cut. •Several hundred different restriction enzymes have been discovered that ...
DNA Structure and Replication
... Review these before coming to class. We will start with DNA replication. DNA structure: fig 11.5, 11.6, 11.7 Double helix: antiparallel, complementary, dNMPs joined by phosphodiester linkage to adjacent dNMPs. Strands have a polarity: 5’ -> 3’ DNA sequence is written from 5’ -> 3’ Hydrogen bonds bet ...
... Review these before coming to class. We will start with DNA replication. DNA structure: fig 11.5, 11.6, 11.7 Double helix: antiparallel, complementary, dNMPs joined by phosphodiester linkage to adjacent dNMPs. Strands have a polarity: 5’ -> 3’ DNA sequence is written from 5’ -> 3’ Hydrogen bonds bet ...
Chapter 12 Study Guide
... 3 is a harmless version of a disease-causing microbe 9 The part of the molecule for which deoxyribonucleic acid is named 12 rules stating that in DNA a purine on one strand always pairs with a pyrimidine on the opposite strand 13 describes a microorganism or virus that causes disease and that is hig ...
... 3 is a harmless version of a disease-causing microbe 9 The part of the molecule for which deoxyribonucleic acid is named 12 rules stating that in DNA a purine on one strand always pairs with a pyrimidine on the opposite strand 13 describes a microorganism or virus that causes disease and that is hig ...
Chapter 12-1: DNA - SandersBiologyStuff
... Francis _____ and James __________ were trying to figure out the structure of DNA by building models with cardboard and wire. When they saw Franklin’s pictures they soon were able piece all the information together to come up with the 3dimensional structure of DNA: a ______________ with two strands ...
... Francis _____ and James __________ were trying to figure out the structure of DNA by building models with cardboard and wire. When they saw Franklin’s pictures they soon were able piece all the information together to come up with the 3dimensional structure of DNA: a ______________ with two strands ...
DNA & Proteins
... National DNA Database Britain has the largest DNA database of its citizens in the world. It holds details of over 4 million people – 5.2% of the UK population is on the database compared with 0.5% in the USA. DNA samples obtained for analysis from the collection of DNA at crime scenes and from ...
... National DNA Database Britain has the largest DNA database of its citizens in the world. It holds details of over 4 million people – 5.2% of the UK population is on the database compared with 0.5% in the USA. DNA samples obtained for analysis from the collection of DNA at crime scenes and from ...
Document
... Guanine and Cytosine – These follow the rules of base-pairing: • Adenine bonds with Thymine • Guanine bonds with Cytosine ...
... Guanine and Cytosine – These follow the rules of base-pairing: • Adenine bonds with Thymine • Guanine bonds with Cytosine ...
Mutations - TeacherWeb
... What do mutations do to the protein? Are they all bad or all good? The genes in your DNA code for a specific ____________________. The ____________ and ____________ of amino acids will determine the ___________ and _________________ of the protein. The DNA sequence below codes for a protein called ...
... What do mutations do to the protein? Are they all bad or all good? The genes in your DNA code for a specific ____________________. The ____________ and ____________ of amino acids will determine the ___________ and _________________ of the protein. The DNA sequence below codes for a protein called ...
Genetic Technology - Solon City Schools
... What macromolecule do you think they are made of? They are PROTEINS that cut ...
... What macromolecule do you think they are made of? They are PROTEINS that cut ...
Chapter 14: DNA Technologies
... 1. Restriction enzymes cut DNA in areas of specific base pair sequences that are palindromic 2. Staggered cuts in palindromic regions leave strands with complementary ("sticky") ends 3. Segments of DNA with "sticky" ends can be joined with DNA ligases 4. Restriction enzymes vary in the number of DNA ...
... 1. Restriction enzymes cut DNA in areas of specific base pair sequences that are palindromic 2. Staggered cuts in palindromic regions leave strands with complementary ("sticky") ends 3. Segments of DNA with "sticky" ends can be joined with DNA ligases 4. Restriction enzymes vary in the number of DNA ...
CHAPTER 18
... and –10: 5’–TATAAT–3’. Most mutations that alter the consensus sequence would be expected to decrease the rate of transcription. For example, a mutation that changed the – 35 region to 5’–GAGACA–3’ would decrease transcription. The sequence 5’–TATAAT–3’ is recognized by the transcription factor TFII ...
... and –10: 5’–TATAAT–3’. Most mutations that alter the consensus sequence would be expected to decrease the rate of transcription. For example, a mutation that changed the – 35 region to 5’–GAGACA–3’ would decrease transcription. The sequence 5’–TATAAT–3’ is recognized by the transcription factor TFII ...
goals - s3.amazonaws.com
... shows the presence(+) or absence (-) of the “Alu” DNA sequence on the paternal and maternal chromosomes. • We will use primers for the Alu sequence that will generate a 550 bp fragment if the Alu sequence is not present and an 850 bp fragment if the Alu sequence is present through the application of ...
... shows the presence(+) or absence (-) of the “Alu” DNA sequence on the paternal and maternal chromosomes. • We will use primers for the Alu sequence that will generate a 550 bp fragment if the Alu sequence is not present and an 850 bp fragment if the Alu sequence is present through the application of ...
DNA STRUCTURE AND REPLICATION Nucleotides: 1. 5 carbon
... Into what phage component was the 35S incorporated?_______________ The phages with the 35S were then allowed to infect E. coli free of 35S. After a period of time, the culture was blended, centrifuged, and analyzed to determine where the location of the 35S in the mixture. Where was the 35S located ...
... Into what phage component was the 35S incorporated?_______________ The phages with the 35S were then allowed to infect E. coli free of 35S. After a period of time, the culture was blended, centrifuged, and analyzed to determine where the location of the 35S in the mixture. Where was the 35S located ...
DNA Cutout Model Activity
... DNA is a special molecule that carries the code for every protein manufactured in your body. The DNA molecule is formed by subunits called nucleotides. There are four different nucleotides, each containing a sugar, phosphate, and nitrogen-containing base. The names of these four nitrogen-containing ...
... DNA is a special molecule that carries the code for every protein manufactured in your body. The DNA molecule is formed by subunits called nucleotides. There are four different nucleotides, each containing a sugar, phosphate, and nitrogen-containing base. The names of these four nitrogen-containing ...
DNA and PROTEIN SYNTHESIS - Salisbury Composite High
... The bacterial Salmonella typhimurium is unable to make histidine, so this amino acid must be supplied for the bacteria to survive. After the bacteria is exposed to a potential mutagen, it is grown in a culture without histidine. If no mutation took place, the bacteria will die due to the lack of his ...
... The bacterial Salmonella typhimurium is unable to make histidine, so this amino acid must be supplied for the bacteria to survive. After the bacteria is exposed to a potential mutagen, it is grown in a culture without histidine. If no mutation took place, the bacteria will die due to the lack of his ...
Genomes 1
... Essential for the control of the host cell Enzyme can be used to manufacture DNA from mRNA Used to reverse manufacture the DNA code for the insulin gene from mRNA ...
... Essential for the control of the host cell Enzyme can be used to manufacture DNA from mRNA Used to reverse manufacture the DNA code for the insulin gene from mRNA ...
plasmid
... • Many cloning vectors contain a multiple cloning site or polylinker: a DNA segment with several unique sites for restriction endo- nucleases located next to each other • Restriction sites of the polylinker are not present anywhere else in the plasmid. • Cutting plasmids with one of the restriction ...
... • Many cloning vectors contain a multiple cloning site or polylinker: a DNA segment with several unique sites for restriction endo- nucleases located next to each other • Restriction sites of the polylinker are not present anywhere else in the plasmid. • Cutting plasmids with one of the restriction ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.