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Go Bananas
Go Bananas

... A simple laboratory practical in which DNA is extracted from bananas using everyday chemicals. DNA is the chemical genes are made from. It contains a code that specifies the amino acid sequences of proteins. Proteins do jobs within cells, and the proteins a cell makes determine what type of cell it ...
VGEC: Teacher Notes Go Bananas!
VGEC: Teacher Notes Go Bananas!

... A simple laboratory practical in which DNA is extracted from bananas using everyday chemicals. DNA is the chemical genes are made from. It contains a code that specifies the amino acid sequences of proteins. Proteins do jobs within cells, and the proteins a cell makes determine what type of cell it ...
answer key
answer key

... Why is there not simply chaos in the cell, with rampant tRNA’s flying all over the place at random? Each tRNA has an anticodon on it – a triplet of bases that perfect complements one, AND ONLY ONE, codon on mRNA. For example, the tRNA with the anticodon UAC will perfectly match a segment of mRNA wit ...
8-DNA
8-DNA

... 16. During the synthesis of each new Okazaki fragment, the following enzymes operate sequentially. A. Next to each protein, add a number to indicate the order in which the proteins function during the synthesis of a new stretch of DNA. ____ DNA ligase - ______________________________________________ ...
DNA Technology
DNA Technology

... to change the information it contains. By changing this information, genetic engineering changes the type or amount of proteins an organism is capable of producing, thus enabling it to make new substances or perform new functions. ...
Antibiotics and resistance
Antibiotics and resistance

... • Diagnosis of microbial diseases: by detecting specific sequences of their DNA. This is especially useful for those pathogens that: • Cannot be cultured or cultured with difficulty e.g. HBV, HCV and HIV. • Those that grow very slowly e.g. M. tuberculosis. • Detection of genes coding for virulence f ...
Chapter 11
Chapter 11

... register, a frame-shift mutation results. • These are extremely detrimental because the final protein intended by the message may be altered or not made. ...
DNA TECHNOLOGY AND THE HUMAN GENOME
DNA TECHNOLOGY AND THE HUMAN GENOME

... • VECTOR  A PLASMID CARRYING EXTRA GENES OTHER THAN THOSE NEEDED FOR REPLICATION AND CONJUGATION • “F” PLASMID NOT THE ONLY PLASMID • “R” PLASMID = RESISTANCE TO ANTI-BIOTICS • PRESENCE OF “R” PLASMID MAY LEAD TO “SUPERBACTERIA” ...
Transposition and transposable elements
Transposition and transposable elements

... have suffered mutation and are no longer active • some of these may have cis-acting end mutations and cannot be mobilized • others may have intact ends but no transposase: these can be mobilized by a element that is tnp+ (“autonomous” element) • Ac Ds system is an example of latter: Ac (activator) c ...
Palindromic Sequences
Palindromic Sequences

... separated through a process known as paper chromatography. A similar process was done for fraction A. From all the studies perform, the overall structure and cross linking between chains can be deduced. Cross-linking can occur on cysteine residues, because they contain sulfur atoms, capable of dimer ...
This would be given at the end of the unit
This would be given at the end of the unit

... 11. A strand of DNA formed by the splicing of DNA from two different species is called a. determinant RNA. b. recombinant DNA. c. plasmid DNA. d. restriction RNA. 12. Plasmids a. are circular pieces of bacterial DNA. b. can replicate independently of the organism’s main chromosome. c. are often used ...
Lab 5 minipreps
Lab 5 minipreps

... proteins that recognize and bind to specific DNA sequences and cut the DNA at or near the recognition site. Restriction enzymes were originally discovered through their ability to break down, or "restrict" foreign DNA. In their natural environment, the bacterial cell, they serve a protective functio ...
D. melanogaster - University of Evansville Faculty Web sites
D. melanogaster - University of Evansville Faculty Web sites

... • probe forms double helix with complementary DNA ...
DNA: The Carrier of Genetic Information
DNA: The Carrier of Genetic Information

Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... • Staggered cuts occur when the recognition sequence usually displays twofold symmetry, palindromes ...
DNA - K.T. Leung
DNA - K.T. Leung

... Restriction enzymes • Each restriction enzymes cuts the DNA into defined fragments by acting at specific target molecules. • These enzymes act as scissors, cutting the DNA into specific sites. • Restriction enzymes are commonly available. ...
chapter14
chapter14

... 1. The plasmid and the DNA from another organism are treated with the same restriction enzyme. 2. Single molecules with complementary single-stranded sticky ends are produced. 3. The two types of molecules are mixed together so that their sticky ends pair. 4. The mixture is then treated with DNA lig ...
DNA - anisam2
DNA - anisam2

... Nucleotides are linked by phosphodiester bonds to form polynucleotides. Phosphodiester bond Covalent bond between the phosphate group (attached to 5’ carbon) of one nucleotide and the 3’ carbon of the sugar of another nucleotide. This bond is very strong, and for this reason DNA is remarkably stab ...
DNA: The Genetic Material
DNA: The Genetic Material

... – synthesize all their molecules from 3 simple starting materials: CO2, H2O, & NH4. 2. To satisfy their need for ATP, plants, like all other organisms, carry out cellular respiration. – Both aerobic respiration and fermentation can occur in plants – respiration is more common. ...
nucleic acid
nucleic acid

... (3) The diameter of the double helix is 2 nm, the distance between two base is 0.34 nm, each turn of the helix involves 10 bases pairs, 3,4 nm. ...
Lab 8
Lab 8

... In our cells, DNA is found inside the nucleus, wrapped around basic protein molecules called histones (kind of like thread wrapped around a spool). This combination of DNA and protein is called a nucleosome. The DNA does not leave the nucleus, so when new proteins or other structures need to be made ...
Gel Electrophoresis of DNA
Gel Electrophoresis of DNA

... • The power source is turned on and the gel is run. The time of the run depends upon the amount of current and % gel, and requires experimentation • At the end of the run the gel is removed (it is actually quite stiff) • The gel is then visualized - UV light causes the bands of DNA to fluoresce ...
DNA Structure and history10
DNA Structure and history10

... • DNA backbone is bonded from phosphate to sugar between 3 & 5 carbons ...
This is a test - DNA Learning Center
This is a test - DNA Learning Center

... Although the DNA from different individuals is more alike than different, there are many regions of the human chromosomes that exhibit a great deal of diversity. Such variable sequences are termed “polymorphic” (meaning many forms) and provide the basis for genetic disease diagnosis, forensic identi ...
02HYD16_Layout 1
02HYD16_Layout 1

... A) Its charged phosphate groups B) The formation of hydrogen bonds between bases from opposite strands C) The opposite direction of the two strands D) The pairing of bases on one strand with bases on the other strand 25. What is antisense technology? A) RNA polymerase producing DNA B) A cell display ...
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DNA repair



DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.
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