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„DNA damage“?
„DNA damage“?

... • the MMR contributes to replication fidelity by a factor of 103 by removal of base-base mismatches, insertions and deletions (hence the resulting incidence of mutations due to erroneous replication is only 1:1010) • the system must be able discrimitate between parental and daughter DNA strand! • Mu ...
DNA - The Double Helix
DNA - The Double Helix

... which in turn codes for a trait. Hence, you hear it commonly referred to as the gene for baldness or the gene for blue eyes. Meanwhile, DNA is the chemical that genes and chromosomes are made of. DNA is called a nucleic acid because it was first found in the nucleus. We now know that DNA is also fou ...
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Document

... the linear DNA phenotype, just like deletion of ruvABC does. Therefore: ruvC may be directly breaking the chromosome. But note that rep recBCTS ruvC is lethal while rep recBCTS ruvABC is fine. So ruvC is lethal only when ruvAB are active. ...
lecture notes-biochemistry-4-Nucleic Acids
lecture notes-biochemistry-4-Nucleic Acids

... • The three major components in all nucleotides are phosphoric acid, pentose (ribose and deoxyribose), and a base (purine or purimidine). • Two major purines present in nucleotides are adenine (A) and guanine (G), and three major purimidines are thymine (T), cytosine (C) and uracil (U). ...
DNA Replication and Protein Synthesis
DNA Replication and Protein Synthesis

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Chapter 17-part 2
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DNA Synthesis Activity for Biology 40S

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DNA: THE CODE OF LIFE Checklist Exam Questions

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DNA - The Double Helix
DNA - The Double Helix

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The role of novel genes... - Sussex Research Online

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Class14 1-25 Win16 DNA Replication Notes

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Molecular Genetics - Ursuline High School
Molecular Genetics - Ursuline High School

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DNA - Southgate Schools

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Ch. 12 DNA
Ch. 12 DNA

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... Cells Divide by the Process of Mitosis Cell with a single copy of DNA DNA replicates to form chromosomes (two copies of DNA) Chromosomes line up in the middle of the cell Chromosomes are split. Half of each chromosome travels to either end of the cell. The cell divides to form two new cells with th ...
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Lecture #7

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DNA_RNA - District 128 Moodle
DNA_RNA - District 128 Moodle

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Quiz-3
Quiz-3

... was cloned using Pst-1 and restriction enzyme and the size of the recombinant plasmid is 5.0 kb. What will you do to confirm that you have the correct insert, and that it is the specific genes you wanted? Indicate the size of the DNA fragments on agarose gel. 23. What is RFLP and how is it useful fo ...
DNA History and Structure - Ms. Ottolini`s Biology Wiki!
DNA History and Structure - Ms. Ottolini`s Biology Wiki!

... pyrimidines because they are so small that they don’t get close enough to each other (given the width of the double helix) to form hydrogen bonds with each other • Purines cannot pair with purines because they are so large that they would not fit within the width of the double helix ...
Describe the process of DNA fingerprinting.
Describe the process of DNA fingerprinting.

... In the last 15 years, DNA has played an increasingly important role in our legal system. Tissue evidence is now routinely collected during criminal investigations in hopes that it will provide genetic clues linking suspected criminals to crimes. DNA profiles help forensic investigators determine whe ...
How many chromosomes?
How many chromosomes?

... Copying DNA • A dividing cell duplicates its DNA – creates 2 copies of all DNA – separates the 2 copies to opposite ends of the cell – splits into 2 daughter cells ...
word - marric
word - marric

... Proteins are polymers composed of amino acid monomers. Different types of proteins function as enzymes, transport molecules, hormones, structural components of cells, and antibodies that fight infection. Most cells in an individual organism carry the same set of DNA instructions but do not use the e ...
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Homologous recombination



Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks. Homologous recombination also produces new combinations of DNA sequences during meiosis, the process by which eukaryotes make gamete cells, like sperm and egg cells in animals. These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses.Although homologous recombination varies widely among different organisms and cell types, most forms involve the same basic steps. After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then ""invades"" a similar or identical DNA molecule that is not broken. After strand invasion, the further sequence of events may follow either of two main pathways discussed below (see Models); the DSBR (double-strand break repair) pathway or the SDSA (synthesis-dependent strand annealing) pathway. Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break.Homologous recombination is conserved across all three domains of life as well as viruses, suggesting that it is a nearly universal biological mechanism. The discovery of genes for homologous recombination in protists—a diverse group of eukaryotic microorganisms—has been interpreted as evidence that meiosis emerged early in the evolution of eukaryotes. Since their dysfunction has been strongly associated with increased susceptibility to several types of cancer, the proteins that facilitate homologous recombination are topics of active research. Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. For their development of this technique, Mario Capecchi, Martin Evans and Oliver Smithies were awarded the 2007 Nobel Prize for Physiology or Medicine.
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