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Experiment #6: DNA Extraction from Fruits
Experiment #6: DNA Extraction from Fruits

... contains the biological instructions that make each species unique. DNA, along with the instructions it contains, is passed from adult organisms to their offspring during reproduction. In complex eukaryotic cells such as plants animal and plant cells, most of the DNA is located in the cell nucleus ( ...
Unit 4
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... 8. Define codon, and explain what relationship exists between the linear sequence of codons on mRNA and the linear sequence of amino acids in a polypeptide. - Each three bases of mRNA are called a codon, these codons line up with the amino acids picked up by the tRNA. 12. Explain the process of tra ...
2013 Charlotte-Mecklenburg Schools Science Elective Resource
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...  CODIS is the acronym for the “Combined DNA Index System” and is the generic term used to describe the FBI’s program of support for criminal justice DNA databases as well as the software used to run these databases. The National DNA Index System or NDIS is considered one part of CODIS, the national ...
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DNA/RNA/Transcription/Translation Notes DNA, RNA, Replication

... molecules Two DNA molecules are identical Semi-Conservative Replication ● Each have one original strand and one new strand ...
DNA Structure DNA Structure
DNA Structure DNA Structure

...  These mistakes or mutations are a mixed blessing. They provide the genetic variation that is essential for evolution but, unfortunately, most are harmful. ...
DNA * Deoxyribonucleic Acid
DNA * Deoxyribonucleic Acid

... • They cut, splice together, & insert the modified DNA molecules from different species into bacteria or another type of cell that rapidly replicates and divides. • The cells copy the foreign DNA right along with their own DNA. • An example of this is the gene for human insulin inserted into a bacte ...
unit iv - dna & cell division
unit iv - dna & cell division

... polymerase can only add nucleotides to the 3’ end  Results in shorter and shorter DNA molecules with jagged ends  To protect genetic integrity, ends of chromosomes do not contain genes – instead there are nucleotide sequences known as telomeres  Contain nucleotide repeat sequences  Telomeres sho ...
Ch. 16 The Molecular Basis of Life
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... ­telomerase catalyzes the lengthening of the   telomeres in germ cells ­restores original length ­uses a short RNA molecule that acts as a   template for new telomere synthesis ­have found telomerase in cancer cells that  are not germ cells ­if we can find a way to stop the telomerase  in tumor cell ...
dna review with key
dna review with key

... Each new molecule contains one strand from the original molecule and one newly synthesized strand. ...
codon - Anoka-Hennepin School District
codon - Anoka-Hennepin School District

... DNA strand from which it was transcribed or the complementary strand that wasn’t used? Explain 3. Explain how the structure of DNA enables the molecule to be easily transcribed. Why is this important for genetic information? 4. Why is RNA important to the cell? How does an mRNA molecule carry inform ...
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... is a closed loop attached to the inner cell membrane. ...
Let`s make some animal proteins using DNA!!
Let`s make some animal proteins using DNA!!

... What is “transcription?” • A part of the DNA double helix within the nucleus is unzipped, cut by enzymes, and then copied onto a new single strand, called mRNA. This process is called “transcription.” • Once the DNA is transcribed, the single strand moves from the nucleus to a ribosome in the cytop ...
Let`s make some animal proteins using DNA!!
Let`s make some animal proteins using DNA!!

... What is “transcription?” • A part of the DNA double helix within the nucleus is unzipped, cut by enzymes, and then copied onto a new single strand, called mRNA. This process is called “transcription.” • Once the DNA is transcribed, the single strand moves from the nucleus to a ribosome in the cytop ...
Document
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... • Knew A - T (2 H bonds), C - G (3 H bonds ...
How are animal proteins made from DNA?
How are animal proteins made from DNA?

... What is “transcription?” • A part of the DNA double helix within the nucleus is unzipped, cut by enzymes, and then copied onto a new single strand, called mRNA. This process is called “transcription.” • Once the DNA is transcribed, the single strand moves from the nucleus to a ribosome in the cytop ...
Discovery of nucleic acid • DNA is made up of:
Discovery of nucleic acid • DNA is made up of:

...  Phosphate groups bonded to sugar groups (backbone)  Antiparallel (strands opposite one another) ...
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Gel Electrophoresis of DNA

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Scientist Reading
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... with radioactive sulfur. One group of bacteria was then exposed to phages with radioactive DNA. Another group was exposed to phages with radioactive protein. After large numbers of bacteria had become infected with phages, the cytoplasm of the bacteria was tested for radioactivity. The cells that ha ...
Ch122008–i only
Ch122008–i only

... of individual units called nucleotides. Three of these units form a code. The order, or sequence, of a code and the type of code determine the meaning of the message. 1. On a sheet of paper, write the word cats. List the letters or units that make up the word cats. 2. Try rearranging the units to fo ...
Strawberry DNA Extraction Lab
Strawberry DNA Extraction Lab

... 4. A person cannot see a single cotton thread 100 feet away, but if you wound thousands of threads together into a rope, it would be visible at some distance. How is this statement an analogy to our DNA extraction? ...
chapt02_lecture
chapt02_lecture

... In 1944 Avery, MacLeod and McCarty - used a transformation test - Griffith’s - care to define the chemical nature of the transforming substance - Techniques used excluded both protein and RNA as the chemical agent of transformation - Other treatments verified that DNA is the chemical agent of transf ...
Transposition - Pennsylvania State University
Transposition - Pennsylvania State University

... • The 3’ ends of each strand from the staggered break (at the target) serve as primers for repair synthesis. • Copying through the transposon followed by ligation leads to formation of a cointegrate structure. • Copying also generates the flanking direct repeats. • The cointegrate is resolved by rec ...
Restriction Enzymes
Restriction Enzymes

... lengths (usually several million bp in length.) • These are the “elephants” in the race through the agarose and cant enter the gel matrix when they are this big. • Restriction enzymes made possible the cutting of DNA into smaller fragments together with their separation and visualization by agarose ...
Lecture 2 DNA Structure
Lecture 2 DNA Structure

... track which entered the E. coli cell during infection. – They grew one batch of T2 phage in the presence of radioactive sulfur, marking the proteins but not DNA. – They grew another batch in the presence of radioactive phosphorus, marking the DNA but not proteins. – They allowed each batch to infect ...
DNA cloning by homologous recombination in Escherichia coli
DNA cloning by homologous recombination in Escherichia coli

... the selectable gene flanked by carrying an E. coli plasmid origin and an antibiotic selectable marker (Sm) gene flanked by two two homology arms. In the pres- oligonucleotide homology arms (green blocks). The linear cloning vectors were PCR amplified using ence of either RecE/RecT or oligonucleotide ...
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Homologous recombination



Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is most widely used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks. Homologous recombination also produces new combinations of DNA sequences during meiosis, the process by which eukaryotes make gamete cells, like sperm and egg cells in animals. These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses.Although homologous recombination varies widely among different organisms and cell types, most forms involve the same basic steps. After a double-strand break occurs, sections of DNA around the 5' ends of the break are cut away in a process called resection. In the strand invasion step that follows, an overhanging 3' end of the broken DNA molecule then ""invades"" a similar or identical DNA molecule that is not broken. After strand invasion, the further sequence of events may follow either of two main pathways discussed below (see Models); the DSBR (double-strand break repair) pathway or the SDSA (synthesis-dependent strand annealing) pathway. Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break.Homologous recombination is conserved across all three domains of life as well as viruses, suggesting that it is a nearly universal biological mechanism. The discovery of genes for homologous recombination in protists—a diverse group of eukaryotic microorganisms—has been interpreted as evidence that meiosis emerged early in the evolution of eukaryotes. Since their dysfunction has been strongly associated with increased susceptibility to several types of cancer, the proteins that facilitate homologous recombination are topics of active research. Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. For their development of this technique, Mario Capecchi, Martin Evans and Oliver Smithies were awarded the 2007 Nobel Prize for Physiology or Medicine.
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