How dormant origins promote complete genome replication
... With these considerations in mind, we recently modelled the behaviour of origin activation within a single 250 kb origin cluster [41]. Origins were assigned a certain initiation probability per unit time and were then activated stochastically during S phase (Figure 4a). Model parameters (mean origin ...
... With these considerations in mind, we recently modelled the behaviour of origin activation within a single 250 kb origin cluster [41]. Origins were assigned a certain initiation probability per unit time and were then activated stochastically during S phase (Figure 4a). Model parameters (mean origin ...
Replication of Nuclear DNA
... On this basis, it appears that the budding yeast ars core sequence may also be part of plant origins, despite the absence of this sequence in S. pombe origins (as discussed above). However, to date, there has been no direct demonstration of this. Further, since no other plant nuclear DNA replication ...
... On this basis, it appears that the budding yeast ars core sequence may also be part of plant origins, despite the absence of this sequence in S. pombe origins (as discussed above). However, to date, there has been no direct demonstration of this. Further, since no other plant nuclear DNA replication ...
Effects of habitat fragmentation and isolation on the genetic
... The Asiatic wild ass (khulan: Equus hemionus), once distributed across Central Asia, continuously declined in both numbers and range during the 19th century (Reading et al. 2001). In the IUCN Equid Action Plan the status of Equus hemionus is classified as “insufficiently known” and the species is li ...
... The Asiatic wild ass (khulan: Equus hemionus), once distributed across Central Asia, continuously declined in both numbers and range during the 19th century (Reading et al. 2001). In the IUCN Equid Action Plan the status of Equus hemionus is classified as “insufficiently known” and the species is li ...
Equilibrium Statistics of Channel-confined DNA
... Introduction Chapter 1. Introduction This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels wi ...
... Introduction Chapter 1. Introduction This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels wi ...
A Highly Efficient Method for the Construction of a Plasmid
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
... library. The new method was based on the addition of complementary single stranded oligomers to cDNA and vector as 5' overhangs and annealing of the 5' overhangs before ligation to increase ligation efficiency. To generate the long, complementary 5' overhangs to cDNA and vector DNA a common un phosp ...
Optimization and the Robustness of BOX A1R PCR for DNA
... fingerprints were generated from Escherichia coli strains isolated from human, duck, goose, gull, and heron sources. Optimization tools (use of restriction enzymes, BSA, DMSO, and storage buffer) were used in an attempt to generate clear, well-defined DNA fingerprints from the Escherichia coli isola ...
... fingerprints were generated from Escherichia coli strains isolated from human, duck, goose, gull, and heron sources. Optimization tools (use of restriction enzymes, BSA, DMSO, and storage buffer) were used in an attempt to generate clear, well-defined DNA fingerprints from the Escherichia coli isola ...
345 - Timstar
... common technique is a “hot start” step, in which the PCR reagents are introduced in the reaction only after the DNA is fully denatured at 94°C. In this experiment, the students will use the PCR-RFLP method to examine the presence of the polymorphism. Students will use the PCR to amplify the polymorp ...
... common technique is a “hot start” step, in which the PCR reagents are introduced in the reaction only after the DNA is fully denatured at 94°C. In this experiment, the students will use the PCR-RFLP method to examine the presence of the polymorphism. Students will use the PCR to amplify the polymorp ...
Deconstructing the Genome: DNA at High Resolution
... round of replication. Modification enzymes also ensure the survival of those few viral DNA molecules that happen to evade digestion. These viral DNA molecules, modified by methylation, then use the host cell’s machinery to produce new viral particles. Biologists have identified a large number of res ...
... round of replication. Modification enzymes also ensure the survival of those few viral DNA molecules that happen to evade digestion. These viral DNA molecules, modified by methylation, then use the host cell’s machinery to produce new viral particles. Biologists have identified a large number of res ...
safety in numbers? deciding when dna alone is enough to convict
... Bay Area in 1972 shared the matching profile was likely over fifty percent.6 The jury found Puckett guilty, and his appeal is pending. Cases like Puckett are part of an emerging phenomenon of “pure cold hit” DNA prosecutions in which the entirety of the government’s case against the suspect, aside f ...
... Bay Area in 1972 shared the matching profile was likely over fifty percent.6 The jury found Puckett guilty, and his appeal is pending. Cases like Puckett are part of an emerging phenomenon of “pure cold hit” DNA prosecutions in which the entirety of the government’s case against the suspect, aside f ...
An attempt to unify the structure of polymerases
... with the three most conserved motifs of DNAmerases/sequences/structure dependent DNA pol as, suggesting that the two polymerase types may share a common tertiary fold, or at least contain similar local tertiary architecture required for similar functions. These motifs Introduction are likely to repr ...
... with the three most conserved motifs of DNAmerases/sequences/structure dependent DNA pol as, suggesting that the two polymerase types may share a common tertiary fold, or at least contain similar local tertiary architecture required for similar functions. These motifs Introduction are likely to repr ...
Separation of DNA Restriction Fragments by Ion
... plays a central role in DNA research. However, when used for preparative purposes electrophoresis has two particularly serious drawbacks: (A) Extraction of the purified material out of the gel is tedious and difficult to accomplish with high yields (1). (B) The purified DNA is often contaminated wit ...
... plays a central role in DNA research. However, when used for preparative purposes electrophoresis has two particularly serious drawbacks: (A) Extraction of the purified material out of the gel is tedious and difficult to accomplish with high yields (1). (B) The purified DNA is often contaminated wit ...
Protein–Nucleic Acid Interactions
... In the idealized A-form of duplex DNA, the major and minor grooves are more equal in width (Figure 10.1a). The major groove of the A-form is comparatively narrower than the B-form, so that the bases of the Aform are more deeply buried within the body of the duplex and are consequently less accessibl ...
... In the idealized A-form of duplex DNA, the major and minor grooves are more equal in width (Figure 10.1a). The major groove of the A-form is comparatively narrower than the B-form, so that the bases of the Aform are more deeply buried within the body of the duplex and are consequently less accessibl ...
Complete
... introduced. The DNA prism separates ~100 kb DNA 1000 times faster than conventional methods, and provides solutions to sample loading and field uniformity problems. A physical model for explaining the complicated separation characteristics of DNA prisms is developed. The model assumes that DNA molec ...
... introduced. The DNA prism separates ~100 kb DNA 1000 times faster than conventional methods, and provides solutions to sample loading and field uniformity problems. A physical model for explaining the complicated separation characteristics of DNA prisms is developed. The model assumes that DNA molec ...
Genome organization of Magnaporthe grisea
... inoculate 50 ml of liquid complete medium (CM) in 250-ml Erlenmeyer flasks. The flasks were incubated at 25—30°C with shaking at 200 rpm using an orbital shaker. After 2—3 days, the cultures were homogenized using a sterile Waring microblender and the sheared mycelium was shaken for 1 additional day ...
... inoculate 50 ml of liquid complete medium (CM) in 250-ml Erlenmeyer flasks. The flasks were incubated at 25—30°C with shaking at 200 rpm using an orbital shaker. After 2—3 days, the cultures were homogenized using a sterile Waring microblender and the sheared mycelium was shaken for 1 additional day ...
SNP
... Polymorphisms (SNPs) the variant sequence type has a frequency of at least 1% in the population. high frequency of SNPs in human genome: estimated ~1 SNP/Kb. ...
... Polymorphisms (SNPs) the variant sequence type has a frequency of at least 1% in the population. high frequency of SNPs in human genome: estimated ~1 SNP/Kb. ...
Safety in Numbers - Deciding When DNA Alone
... Bay Area in 1972 shared the matching profile was likely over fifty percent. 6 The jury found Puckett guilty, and his appeal is pending. Cases like Puckett are part of an emerging phenomenon of "pure cold hit" DNA prosecutions in which the entirety of the government's case against the suspect, aside ...
... Bay Area in 1972 shared the matching profile was likely over fifty percent. 6 The jury found Puckett guilty, and his appeal is pending. Cases like Puckett are part of an emerging phenomenon of "pure cold hit" DNA prosecutions in which the entirety of the government's case against the suspect, aside ...
Non-invasive prenatal assessment of trisomy 21 by multiplexed
... specificity values of the sequencing protocols based on the predefined cut-off point—namely, chromosome 21 z score of 3. We plotted the receiver operating characteristic (ROC) curves for the measurements of percentage chromosome 21 and determined the areas under the curves (AUC). ROC curve analysis ...
... specificity values of the sequencing protocols based on the predefined cut-off point—namely, chromosome 21 z score of 3. We plotted the receiver operating characteristic (ROC) curves for the measurements of percentage chromosome 21 and determined the areas under the curves (AUC). ROC curve analysis ...
Methods for detection of point mutations
... the type of base exchange on sensitivity [21]. In comparison with DNA-SSCP, higher sensitivities, especially for analysis of fragments of .200 bp, have been reported for RNA-SSCP [13]. Limit of detection. Approximately one mutant cell is detectable in the presence of 10 normal cells [22]. Methods of ...
... the type of base exchange on sensitivity [21]. In comparison with DNA-SSCP, higher sensitivities, especially for analysis of fragments of .200 bp, have been reported for RNA-SSCP [13]. Limit of detection. Approximately one mutant cell is detectable in the presence of 10 normal cells [22]. Methods of ...
2- pcr primer design and reaction optimisation
... dramatically improved the technological abilities of basic and applied researchers in various fields of life sciences. It will not be an exaggeration to say that now no research related to DNA can be performed without the employment of DNA amplification procedures. Newly-developed protocols includin ...
... dramatically improved the technological abilities of basic and applied researchers in various fields of life sciences. It will not be an exaggeration to say that now no research related to DNA can be performed without the employment of DNA amplification procedures. Newly-developed protocols includin ...
2 - cellbiochem.ca
... • Antibiotic resistance gene: allow for selection for bacterial cells that have taken up the vector ...
... • Antibiotic resistance gene: allow for selection for bacterial cells that have taken up the vector ...
PTC PCR II Manual
... extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product is digested with the restriction enzyme HaeIII, whose recognition PTC II - 3 ...
... extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product is digested with the restriction enzyme HaeIII, whose recognition PTC II - 3 ...
Mitochondrial DNA and aging
... levels of lipophilic carcinogens such as polycyclic aromatic hydrocarbons [22,23]. When cells are exposed to some of these compounds, mtDNA is damaged preferentially [24]. Other mutagenic chemicals also have been shown to preferentially target mtDNA [23,25–29]. Therefore it is conceivable that life- ...
... levels of lipophilic carcinogens such as polycyclic aromatic hydrocarbons [22,23]. When cells are exposed to some of these compounds, mtDNA is damaged preferentially [24]. Other mutagenic chemicals also have been shown to preferentially target mtDNA [23,25–29]. Therefore it is conceivable that life- ...
structure and mechanism of dna polymerases
... The viability of an organism is dependent on the accurate replication of its genome. In general, this is performed with only one error generated for every 109–1010 bases replicated (Echols and Goodman, 1991). This remarkable accuracy is achieved by a combination of different mechanisms working in un ...
... The viability of an organism is dependent on the accurate replication of its genome. In general, this is performed with only one error generated for every 109–1010 bases replicated (Echols and Goodman, 1991). This remarkable accuracy is achieved by a combination of different mechanisms working in un ...
Principles and challenges of genome
... frequently in the genome but relatively rarely within CpG islands56. This digestion step is often incorporated into protocols to deplete the sample of CpG-poor DNA before further processing, such as adaptor ligation. small genomes, such as the A. thaliana genome, do not require an amplification step ...
... frequently in the genome but relatively rarely within CpG islands56. This digestion step is often incorporated into protocols to deplete the sample of CpG-poor DNA before further processing, such as adaptor ligation. small genomes, such as the A. thaliana genome, do not require an amplification step ...
A structural determinant in the uracil DNA glycosylase superfamily
... pairs and single-stranded uracil-containing DNA (2,5). Escherichia coli MUG, which belongs to family 2, is named for its UDG activity on mismatched T/U, G/U and C/U base pairs (8–11). In addition to its UDG activity on mismatched base pairs, E. coli MUG is also a robust xanthine DNA glycosylase (12) ...
... pairs and single-stranded uracil-containing DNA (2,5). Escherichia coli MUG, which belongs to family 2, is named for its UDG activity on mismatched T/U, G/U and C/U base pairs (8–11). In addition to its UDG activity on mismatched base pairs, E. coli MUG is also a robust xanthine DNA glycosylase (12) ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.