DNA breathing dynamics distinguish binding from nonbinding
... fragments contain the flanking sequence (CCT) on both ends to minimize end wobbling. The gel shift results are consistent between three independent experiments. The gel shift reactions are conducted at 37 C. RESULTS LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG ...
... fragments contain the flanking sequence (CCT) on both ends to minimize end wobbling. The gel shift results are consistent between three independent experiments. The gel shift reactions are conducted at 37 C. RESULTS LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG ...
KARNATAKA COMMON ENTRANCE TEST – MAY, 2016 BIOLOGY
... Disclaimer: These solutions are provided by the academic team of BASE. These solutions may be different from those to be provided by the Karnataka Examinations Authority (KEA) due to difference in assumptions taken in support of the solutions. In such cases solution provided by KEA will be deemed as ...
... Disclaimer: These solutions are provided by the academic team of BASE. These solutions may be different from those to be provided by the Karnataka Examinations Authority (KEA) due to difference in assumptions taken in support of the solutions. In such cases solution provided by KEA will be deemed as ...
Using the DNA Profile as the Unique Patient Identifier in the
... transferred. Provider departments that complete tests without ever seeing the patient may receive specimens labeled by patient name, financial account number, or medical record number, instead of the department's unique patient identifier. If providers use the patient's initials rather than the pati ...
... transferred. Provider departments that complete tests without ever seeing the patient may receive specimens labeled by patient name, financial account number, or medical record number, instead of the department's unique patient identifier. If providers use the patient's initials rather than the pati ...
Use of novel assays to measure in vivo base excision DNA repair
... The BER system consists of a group of lesion-specific enzymes termed DNA glycosylases, which recognize and excise specific base modifications, resulting in AP sites. Other enzymes act in a series of steps to fill in the missing base, and ultimately restore the correct sequence (Krokan, Nilsen, Skor ...
... The BER system consists of a group of lesion-specific enzymes termed DNA glycosylases, which recognize and excise specific base modifications, resulting in AP sites. Other enzymes act in a series of steps to fill in the missing base, and ultimately restore the correct sequence (Krokan, Nilsen, Skor ...
Collaborative coupling between polymerase and helicase for
... DNA synthesis by a holoenzyme on a DNA hairpin presents two phases. Initially, the holoenzyme has to open a base pair to incorporate a new nucleotide (strand displacement synthesis activity). This phase gives rise to a large change in extension, typically 0.8 nm for a nucleotide incorporated at 10 ...
... DNA synthesis by a holoenzyme on a DNA hairpin presents two phases. Initially, the holoenzyme has to open a base pair to incorporate a new nucleotide (strand displacement synthesis activity). This phase gives rise to a large change in extension, typically 0.8 nm for a nucleotide incorporated at 10 ...
biotransformation - University of California, Berkeley
... Nitrosamines are in tobacco smoke, but also can be formed in beer, fish, meats, and chesses that use nitrite as a preservative. ...
... Nitrosamines are in tobacco smoke, but also can be formed in beer, fish, meats, and chesses that use nitrite as a preservative. ...
NST110: Advanced Toxicology Lecture 4: Phase I Metabolism
... Nitrosamines are in tobacco smoke, but also can be formed in beer, fish, meats, and chesses that use nitrite as a preservative. ...
... Nitrosamines are in tobacco smoke, but also can be formed in beer, fish, meats, and chesses that use nitrite as a preservative. ...
DNA methylation controls histone H3 lysine 9 methylation
... and 5S rDNA) co-localized with chromocenters in wildtype and mutants, and thus remained within the heterochromatin of ddm1 and met1 nuclei. However, BAC DNA clones that represent sequences from the pericentromeric regions hybridized exclusively with chromocenters in the wild type, but showed a dispe ...
... and 5S rDNA) co-localized with chromocenters in wildtype and mutants, and thus remained within the heterochromatin of ddm1 and met1 nuclei. However, BAC DNA clones that represent sequences from the pericentromeric regions hybridized exclusively with chromocenters in the wild type, but showed a dispe ...
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl)-5[5-(4-methylpiperazine-1-yl) benzimidazo-2-yl]-benzimidazole. Hoechst 33342 contains additional ethyl gr ...
... on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl)-5[5-(4-methylpiperazine-1-yl) benzimidazo-2-yl]-benzimidazole. Hoechst 33342 contains additional ethyl gr ...
Charge Transport in DNA - Insights from
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
Forensic Sciences
... evidence and solve crimes. Students are commonly exposed to crime situations, both of a fictional and real nature, through the media. Many current television programs and popular authors use the science of forensics to develop their dramas. However, the methodology used by law enforcement personnel ...
... evidence and solve crimes. Students are commonly exposed to crime situations, both of a fictional and real nature, through the media. Many current television programs and popular authors use the science of forensics to develop their dramas. However, the methodology used by law enforcement personnel ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
... transmission of genetic material. Mitochondrial DNA is no exception; it also forms nucleoprotein complexes, which have become the subject of intense study in recent years. (37) Mitochondrial nucleoprotein complexes, or mitochondrial nucleoids (mt-nucleoids), are believed to contain multi-copy mitoch ...
... transmission of genetic material. Mitochondrial DNA is no exception; it also forms nucleoprotein complexes, which have become the subject of intense study in recent years. (37) Mitochondrial nucleoprotein complexes, or mitochondrial nucleoids (mt-nucleoids), are believed to contain multi-copy mitoch ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... (14, 15), Dryas spp. (23), Alnus spp., and Myrica spp. (17, 26, 27). With some exceptions, most procedures have required the use of relatively large quantities of nodule tissue for DNA isolation, and PCR amplification has usually been limited to short segments of 16S ribosomal DNA (rDNA). Two contin ...
... (14, 15), Dryas spp. (23), Alnus spp., and Myrica spp. (17, 26, 27). With some exceptions, most procedures have required the use of relatively large quantities of nodule tissue for DNA isolation, and PCR amplification has usually been limited to short segments of 16S ribosomal DNA (rDNA). Two contin ...
Applied and Environmental Microbiology
... Proteolytic bacteria are of central importance in nitrogen mineralization. There is evidence that proteases from Bacillus cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus ...
... Proteolytic bacteria are of central importance in nitrogen mineralization. There is evidence that proteases from Bacillus cereus and B. mycoides, which belong to the neutral metalloprotease class, play an important role in proteolytic processes in soils (6, 16–18). The DNA sequence of the B. cereus ...
Recombinant DNA technology and molecular cloning
... foreign DNA thus becomes amplified in number, and following its amplification can be purified for further analysis. ...
... foreign DNA thus becomes amplified in number, and following its amplification can be purified for further analysis. ...
Unraveling DNA helicases
... be the distinction between the SF1 and SF2 family. Another group called family 4 (F4) contains five motifs (1, 1a, 2–4) [3]. The seven motifs of SF1 and SF2 are usually clustered in a region of 200–700 amino acids, the core region. These conserved motifs are separated by stretches of low sequence but ...
... be the distinction between the SF1 and SF2 family. Another group called family 4 (F4) contains five motifs (1, 1a, 2–4) [3]. The seven motifs of SF1 and SF2 are usually clustered in a region of 200–700 amino acids, the core region. These conserved motifs are separated by stretches of low sequence but ...
Revisiting Plus-Strand DNA Synthesis in Retroviruses and Long
... as the first g:C base pair upstream of the PPT/U3 junction (note that throughout the manuscript, RNA and DNA bases have been represented in lower and upper case, respectively). Collectively designated the “unzipped” portion, X-ray crystallography provided the first notion that PPT structural malleab ...
... as the first g:C base pair upstream of the PPT/U3 junction (note that throughout the manuscript, RNA and DNA bases have been represented in lower and upper case, respectively). Collectively designated the “unzipped” portion, X-ray crystallography provided the first notion that PPT structural malleab ...
Molecular events during translocation and proofreading extracted
... based on molecular distance geometry (12), the chief computational algorithm in nucleic magnetic resonance spectroscopy. These computed structures at atomic resolution may reveal short-lived intermediate conformation hard to be captured in crystal lattices. This structural meta-analysis has been app ...
... based on molecular distance geometry (12), the chief computational algorithm in nucleic magnetic resonance spectroscopy. These computed structures at atomic resolution may reveal short-lived intermediate conformation hard to be captured in crystal lattices. This structural meta-analysis has been app ...
A molecular method for assessing meiofauna diversity in marine
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
... Sediment samples were collected from East Beach at Fort DeSoto Park, St. Petersburg, FL, in 1.5mL microcentrifuge tubes and stored at -80°C. DNA was extracted from the sediment using an SDS-based extraction buffer and series of phenol, phenolchloroform, and chloroform extractions, and ethanol-precip ...
... E420-specific bands along with two RFLP markers (Diers et al. 1992) relative to the Rps1 locus was conducted on 54 susceptible F2 plants of the cross Elgin × E420. All RAPD markers along with two RFLP markers were positioned on one side of Rps1 (Fig. 1; Table 1). RAPD271 and RAPD217 were converted t ...
Transparent DNA/RNA Co-extraction Workflow Protocol Suitable for
... steps in the manufacturer’s recommended procedure difficult. Surprisingly, enzymatic treatments are often performed before purification procedures are complete, which we have identified here as a major problem when seeking efficient genomic DNA removal from RNA extracts. Here, we tested several DNA/ ...
... steps in the manufacturer’s recommended procedure difficult. Surprisingly, enzymatic treatments are often performed before purification procedures are complete, which we have identified here as a major problem when seeking efficient genomic DNA removal from RNA extracts. Here, we tested several DNA/ ...
Recent Advances in Developing Small Molecules Targeting Nucleic
... Nucleic acids play significant roles in variety kinds of biological processes [1–5]. According to the differences on sugar scaffold, nucleic acids can be classified into two categories. DNAs and RNAs participate in gene storage, replication, transcription and other important biological activities. T ...
... Nucleic acids play significant roles in variety kinds of biological processes [1–5]. According to the differences on sugar scaffold, nucleic acids can be classified into two categories. DNAs and RNAs participate in gene storage, replication, transcription and other important biological activities. T ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.