DNA denaturation in the rodlike polyelectrolyte model
... stabilize the duplex DNA [1, 2, 27, 28]. Intercalators as ethidium bromide and daunomycin in the presence of monovalent salt induce the denaturation [29, 30] what suggests that the denaturation depends not only on the electrostatic interaction. As result, the balancing between salt concentration and ...
... stabilize the duplex DNA [1, 2, 27, 28]. Intercalators as ethidium bromide and daunomycin in the presence of monovalent salt induce the denaturation [29, 30] what suggests that the denaturation depends not only on the electrostatic interaction. As result, the balancing between salt concentration and ...
DNA SEQUENCING (using a Li
... methods, and their derivations. Both methods were first described in 1977. The first method (Maxam and Gilbert 1977) is based on specific chemical degradation of the DNA. The DNA is first end-labeled using 35s or 33P, followed by separation of the two strands on a gel. Four aliquots of the desired s ...
... methods, and their derivations. Both methods were first described in 1977. The first method (Maxam and Gilbert 1977) is based on specific chemical degradation of the DNA. The DNA is first end-labeled using 35s or 33P, followed by separation of the two strands on a gel. Four aliquots of the desired s ...
PDF
... 1981). Results from several embryo injection studies using different templates led to the conclusion that circular DNA persists and eventually may be amplified through early embryogenesis, but becomes degraded after the gastrula stage. However, it was also found that a small number of injected gene ...
... 1981). Results from several embryo injection studies using different templates led to the conclusion that circular DNA persists and eventually may be amplified through early embryogenesis, but becomes degraded after the gastrula stage. However, it was also found that a small number of injected gene ...
From cheek swabs to consensus sequences: an A to Z protocol for
... seen as increasingly necessary to obtain complete mitochondrial genomes. This is especially true in studies of humans, but for other animal species also [3]. The use of complete mtDNA genomes can help mitigate the reduced phylogenetic resolution, homoplasy and ascertainment bias that is otherwise en ...
... seen as increasingly necessary to obtain complete mitochondrial genomes. This is especially true in studies of humans, but for other animal species also [3]. The use of complete mtDNA genomes can help mitigate the reduced phylogenetic resolution, homoplasy and ascertainment bias that is otherwise en ...
Influence of the environment and probes on rapid DNA sequencing
... by sampling the current with the driving field off. The distributions of these currents, with this particular pore geometry, for all four bases are shown in the top section of Figure 3, assuming each current is measured instantaneously.∗∗ We can see that these distributions are unique, but overlappi ...
... by sampling the current with the driving field off. The distributions of these currents, with this particular pore geometry, for all four bases are shown in the top section of Figure 3, assuming each current is measured instantaneously.∗∗ We can see that these distributions are unique, but overlappi ...
Quantitative real-time PCR assay for rapid detection of
... transcribed spacer (ITS) region (Babu et al. 2007). Even though primers showed high specificity, dot-blot hybridization assays with oligonucleotide probes were not sufficiently sensitive. Furthermore the dot-blot technique is time consuming and subject to cross reactivity under field trials. Quantit ...
... transcribed spacer (ITS) region (Babu et al. 2007). Even though primers showed high specificity, dot-blot hybridization assays with oligonucleotide probes were not sufficiently sensitive. Furthermore the dot-blot technique is time consuming and subject to cross reactivity under field trials. Quantit ...
1495/Chapter 07
... Replication is initiated at hundreds or even thousands of replication origins at any one time. Replication continues until all the replication bubbles have met and the two new DNA molecules separate from each other. The packaging of chromatin means that individual replication forks proceed much more ...
... Replication is initiated at hundreds or even thousands of replication origins at any one time. Replication continues until all the replication bubbles have met and the two new DNA molecules separate from each other. The packaging of chromatin means that individual replication forks proceed much more ...
Notes 1 DNA and RNA
... green plants. These genomes are not inherited in a medallion fashion like chromosomes in the nucleus, but instead are inherited solely from the mother with the other cytoplasmic organelles. MITOCHONDRIAL DNA (mtDNA) MtDNA is often circular, double-stranded and lacking in the structural proteins of t ...
... green plants. These genomes are not inherited in a medallion fashion like chromosomes in the nucleus, but instead are inherited solely from the mother with the other cytoplasmic organelles. MITOCHONDRIAL DNA (mtDNA) MtDNA is often circular, double-stranded and lacking in the structural proteins of t ...
Molecular Structure of the GARP Family of Plant Myb
... assay was performed with a set of synthetic 34-bp oligonucleotides, each containing the core sequence (AGAT) or its variant (GGAT), as shown in Figure 5A. The presumed minimum DNA binding domain of 64 amino acids (i.e., ARR10-B) was used for the gel-shift assays. The results are shown in Figure 5A. ...
... assay was performed with a set of synthetic 34-bp oligonucleotides, each containing the core sequence (AGAT) or its variant (GGAT), as shown in Figure 5A. The presumed minimum DNA binding domain of 64 amino acids (i.e., ARR10-B) was used for the gel-shift assays. The results are shown in Figure 5A. ...
Lesson 3: Can you taste PTC?
... PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product ...
... PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product ...
Development of a qPCR Method to Measure Mitochondrial
... gold standard for measuring cellular DNA damage. However, researchers are exploring PCR-based approaches, where DNA damage results in the disruption of DNA polymerase activity and impaired DNA synthesis. Early investigations used semi-quantitative PCR approaches with target amplicons of between ~450 ...
... gold standard for measuring cellular DNA damage. However, researchers are exploring PCR-based approaches, where DNA damage results in the disruption of DNA polymerase activity and impaired DNA synthesis. Early investigations used semi-quantitative PCR approaches with target amplicons of between ~450 ...
Chapter 21 - University of Hawaii
... 26. Which of the following is NOT true about DNA? A.It forms a double helix. B.Bases are held together by hydrogen bonds. C.Bases form complementary pairs. D.It has a deoxyribose sugar. E.It contains adenine, guanine, cytosine, and uracil. 27. Which of the following is NOT true about mRNA? A.It is ...
... 26. Which of the following is NOT true about DNA? A.It forms a double helix. B.Bases are held together by hydrogen bonds. C.Bases form complementary pairs. D.It has a deoxyribose sugar. E.It contains adenine, guanine, cytosine, and uracil. 27. Which of the following is NOT true about mRNA? A.It is ...
Syllabus for Niagara Falls High School NUSTEP Forensic Class
... identify the methods by which a crime scene is documented demonstrate proper technique in collecting and packaging trace evidence describe how evidence from a crime scene is analyzed Chapter 3 The Study of Hair identify the various parts of a hair describe variations in the structure of th ...
... identify the methods by which a crime scene is documented demonstrate proper technique in collecting and packaging trace evidence describe how evidence from a crime scene is analyzed Chapter 3 The Study of Hair identify the various parts of a hair describe variations in the structure of th ...
2007 - life.illinois.edu
... repressor is inactive at 42° and so does not prevent lysis by the infecting phage. The lack of cI in the recipient lysogen leads to accumulation of cro protein. Cro protein prevents transcription of cI, both in the lysogen and in the incoming phage. This ensures that incoming phage will only be able ...
... repressor is inactive at 42° and so does not prevent lysis by the infecting phage. The lack of cI in the recipient lysogen leads to accumulation of cro protein. Cro protein prevents transcription of cI, both in the lysogen and in the incoming phage. This ensures that incoming phage will only be able ...
greenland ancient dna - Arctic and Alpine Research Group
... the order Rosales, the family Salicaceae, and the genus Saxifraga (Table 1). This result is consistent with the John Evans Glacier forming no more than a few thousand years ago in a high Arctic environment (18), characterized by low plant diversity and sparse vegetation cover similar to that current ...
... the order Rosales, the family Salicaceae, and the genus Saxifraga (Table 1). This result is consistent with the John Evans Glacier forming no more than a few thousand years ago in a high Arctic environment (18), characterized by low plant diversity and sparse vegetation cover similar to that current ...
A small organic compound enhances the religation reaction of
... Et-KuQ acid-base titration The spectrophothometric titration of a 3.5×10 − 5 mol/l aqueous solution of Et-KuQ was performed with a Shimadzu UV-2450 spectrophotometer. The various pH values were obtained by diluting HCl or NaOH mother solutions and measuring the desired pH value through a pH metre (A ...
... Et-KuQ acid-base titration The spectrophothometric titration of a 3.5×10 − 5 mol/l aqueous solution of Et-KuQ was performed with a Shimadzu UV-2450 spectrophotometer. The various pH values were obtained by diluting HCl or NaOH mother solutions and measuring the desired pH value through a pH metre (A ...
A physical map of the genome of Hmmophilus
... Digestion of DNA in agarose blocks. Usually digests were carried out on the DNA contained in one-third of a complete plug. Restriction einzyme buffers were diffused into the agarose blocks as outlined below. Plugs or portions of plugs were washed in Eppendorf tubes with 500 1.11 vlolumesof buffer (u ...
... Digestion of DNA in agarose blocks. Usually digests were carried out on the DNA contained in one-third of a complete plug. Restriction einzyme buffers were diffused into the agarose blocks as outlined below. Plugs or portions of plugs were washed in Eppendorf tubes with 500 1.11 vlolumesof buffer (u ...
DNA-dependent protein kinase in nonhomologous end joining: a
... DNA double-strand breaks (DSBs) can be accidentally introduced in cells by the action of ionizing radiation or certain reactive radicals. These agents have the ability to initiate a series of chemical reactions that ultimately sever the DNA backbone, resulting in chromosome breakage and fragmentatio ...
... DNA double-strand breaks (DSBs) can be accidentally introduced in cells by the action of ionizing radiation or certain reactive radicals. These agents have the ability to initiate a series of chemical reactions that ultimately sever the DNA backbone, resulting in chromosome breakage and fragmentatio ...
Genetics and Biotechnology
... An electric current is used to separate DNA fragments according to the size of the fragments in a process called gel electrophoresis. When an electric current is applied, the DNA fragments move toward the positive end of the gel. The smaller fragments move farther faster than the larger ones. ...
... An electric current is used to separate DNA fragments according to the size of the fragments in a process called gel electrophoresis. When an electric current is applied, the DNA fragments move toward the positive end of the gel. The smaller fragments move farther faster than the larger ones. ...
Chapter 16 The Molecular Basis of Inheritance Multiple
... 7) For a science fair project, two students decided to repeat the Hershey and Chase experiment, with modifications. They decided to label the nitrogen of the DNA, rather than the phosphate. They reasoned that each nucleotide has only one phosphate and two to five nitrogens. Thus, labeling the nitrog ...
... 7) For a science fair project, two students decided to repeat the Hershey and Chase experiment, with modifications. They decided to label the nitrogen of the DNA, rather than the phosphate. They reasoned that each nucleotide has only one phosphate and two to five nitrogens. Thus, labeling the nitrog ...
from Saccharomyces cerevisiae
... fragment that can be selectively excised from the chromatin DNA with restriction endonucleases. CEN3 DNA is organized in chromatin such that a 220-250-bp region encompassing the elements of centromere homology is resistant to nuclease digestion. We have localized the functional centromere core to a ...
... fragment that can be selectively excised from the chromatin DNA with restriction endonucleases. CEN3 DNA is organized in chromatin such that a 220-250-bp region encompassing the elements of centromere homology is resistant to nuclease digestion. We have localized the functional centromere core to a ...
Therapeutic Targeting of the DNA Mismatch Repair Pathway
... MSI-low (MSI-L) in which one of the five markers are unstable, and microsatellite stable (MSS) in which none of the five markers show instability (33). MSI-low tumors have also been classified as MSS, and the difference between MSI-L and MSS seems to be largely quantitative. The standard adjuvant tr ...
... MSI-low (MSI-L) in which one of the five markers are unstable, and microsatellite stable (MSS) in which none of the five markers show instability (33). MSI-low tumors have also been classified as MSS, and the difference between MSI-L and MSS seems to be largely quantitative. The standard adjuvant tr ...
DNA: THE INDISPENSIBLE FORENSIC SCIENCE TOOL
... DNA at Work • DNA directs the production of proteins, which are made by combining amino acids. • The sequence of amino acids in a protein chain determines the shape and function of the protein. • Each group of three nucleotides in a DNA sequence codes for a particular amino acid. – Example: G-A-G c ...
... DNA at Work • DNA directs the production of proteins, which are made by combining amino acids. • The sequence of amino acids in a protein chain determines the shape and function of the protein. • Each group of three nucleotides in a DNA sequence codes for a particular amino acid. – Example: G-A-G c ...
Uracil in DNA
... number of different antibodies that are trained to protect us from specific pathogens. To increase the number of different antibodies that can be created, we shuffle the DNA sequence in the regions that code for them, not only by recombining the existing sequences in the cells but also by creating n ...
... number of different antibodies that are trained to protect us from specific pathogens. To increase the number of different antibodies that can be created, we shuffle the DNA sequence in the regions that code for them, not only by recombining the existing sequences in the cells but also by creating n ...
Unit 10.1.4 - Measuring Genetic Variation using Molecular Markers
... • Co-migration: same mobility, same protein? An assumption commonly made when comparing profiles is that proteins which share the same mobility and intensity in a gel are homologous proteins, that is, that they are products of the same gene(s). This is a questionable assumption, especially if only o ...
... • Co-migration: same mobility, same protein? An assumption commonly made when comparing profiles is that proteins which share the same mobility and intensity in a gel are homologous proteins, that is, that they are products of the same gene(s). This is a questionable assumption, especially if only o ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.