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Distinct mechanisms of DNA repair in mycobacteria - MCBL
... leprosy. It is estimated that about a third of the human population may be latently infected with Mycobacterium tuberculosis resulting in two million deaths annually (Dye et al., 1999). The biological niche of the pathogenic mycobacteria is the host macrophages. Pathogen’s ability to sustain within ...
... leprosy. It is estimated that about a third of the human population may be latently infected with Mycobacterium tuberculosis resulting in two million deaths annually (Dye et al., 1999). The biological niche of the pathogenic mycobacteria is the host macrophages. Pathogen’s ability to sustain within ...
Pursuing DNA Catalysts for Protein Modification
... the sequence contexts of, e.g., two serine side chain hydroxyl groups when presented on a protein surface. Also, smallmolecule catalysts may not tolerate the aqueous conditions required to function with most protein substrates. Evolved protein enzymes have achieved many successes, but if nature has ...
... the sequence contexts of, e.g., two serine side chain hydroxyl groups when presented on a protein surface. Also, smallmolecule catalysts may not tolerate the aqueous conditions required to function with most protein substrates. Evolved protein enzymes have achieved many successes, but if nature has ...
Day and Sweatt
... demethylation10,30. Moreover, it appears that DNMTs may be involved in deamination of MeC in a strand-specific manner29, which would implicate them in both the methylation and demethylation of DNA. Although it remains unclear whether this model could account for demethylation of both DNA strands, th ...
... demethylation10,30. Moreover, it appears that DNMTs may be involved in deamination of MeC in a strand-specific manner29, which would implicate them in both the methylation and demethylation of DNA. Although it remains unclear whether this model could account for demethylation of both DNA strands, th ...
Gel Electrophoresis
... In contrast to the agarose gel electrophoresis, PAGE is usually run vertically (Figure 10). SDS-PAGE is carried out with two different types of polyacrylamide gels fused together. The gel is thus composed of two parts, the "stacking gel" and the "resolving gel". The role of the stacking gel is to co ...
... In contrast to the agarose gel electrophoresis, PAGE is usually run vertically (Figure 10). SDS-PAGE is carried out with two different types of polyacrylamide gels fused together. The gel is thus composed of two parts, the "stacking gel" and the "resolving gel". The role of the stacking gel is to co ...
The Roles of the Quorum-Sensing System in the Release of
... reaction for PAO-JP2 when it was exposed to a mAb against P. aeruginosa serotype O5 (data not shown). However, a reaction to the mAb was detected in the PAO-JP2 samples cultured with C12-HSL. This band was not detected when C4HSL was used instead of C12-HSL. These results indicate that when the las ...
... reaction for PAO-JP2 when it was exposed to a mAb against P. aeruginosa serotype O5 (data not shown). However, a reaction to the mAb was detected in the PAO-JP2 samples cultured with C12-HSL. This band was not detected when C4HSL was used instead of C12-HSL. These results indicate that when the las ...
101. The Role of Rigidity in DNA Looping
... of the Escherichia coli L-arabinose operon. In the absence of arabinose, AraC prefers to loop DNA by binding to two half-sites that are separated by 210 base pairs, and in the presence of arabinose it prefers to bind to adjacently located half-sites. The basis for this ligand-regulated shift in bind ...
... of the Escherichia coli L-arabinose operon. In the absence of arabinose, AraC prefers to loop DNA by binding to two half-sites that are separated by 210 base pairs, and in the presence of arabinose it prefers to bind to adjacently located half-sites. The basis for this ligand-regulated shift in bind ...
M3 Multiplex Master Mix – PCR (2x)
... of more bands, but also of additional, non-specific bands). Decreasing the MgCl2 concentration decreases PCR yield but enhances reaction specificity (less bands, but specific PCR products). 4. Primer concentration: A final primer concentration of 0.2 μM for each single primer is usually optimal, but ...
... of more bands, but also of additional, non-specific bands). Decreasing the MgCl2 concentration decreases PCR yield but enhances reaction specificity (less bands, but specific PCR products). 4. Primer concentration: A final primer concentration of 0.2 μM for each single primer is usually optimal, but ...
Using a Single-Nucleotide Polymorphism to Predict
... PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product ...
... PROZAC® and Paxil®. In this experiment, a sample of human cells is obtained by saline mouthwash. DNA is extracted by boiling with Chelex resin, which binds contaminating metal ions. Polymerase chain reaction (PCR) is then used to amplify a short region of the TAS2R38 gene. The amplified PCR product ...
Roles of the Amino Group of Purine Bases in the Thermodynamic
... important for the creation of secondary and higher-order structures and the practical application of oligonucleotides for hybridization-based detection and targeting assays. However, the small differences between the interaction energies of complementary and mismatched pairs cause a difficulty in di ...
... important for the creation of secondary and higher-order structures and the practical application of oligonucleotides for hybridization-based detection and targeting assays. However, the small differences between the interaction energies of complementary and mismatched pairs cause a difficulty in di ...
The “Starch Wars” and the Early History of DNA Profiling
... separated using protein electrophoresis. Just as in electrophoresis of DNA, protein electrophoresis involved the separation of molecules with different surface charges in a porous gel-like substance subjected to constant current. Although the separation of these polymorphic protein enzymes was relat ...
... separated using protein electrophoresis. Just as in electrophoresis of DNA, protein electrophoresis involved the separation of molecules with different surface charges in a porous gel-like substance subjected to constant current. Although the separation of these polymorphic protein enzymes was relat ...
Human Pif1 helicase is a G-quadruplex DNA
... G4 DNA was more efficiently unwound than T55-OX G4 DNA by hPifHD, whereas the converse was observed with hPif1, the full-length enzyme. The V4 annealing product, designated G4* in Figure 1(B), was also resolved by the enzyme (results not shown). As shown in Figure 2(B), the efficiency of G4 DNA unwi ...
... G4 DNA was more efficiently unwound than T55-OX G4 DNA by hPifHD, whereas the converse was observed with hPif1, the full-length enzyme. The V4 annealing product, designated G4* in Figure 1(B), was also resolved by the enzyme (results not shown). As shown in Figure 2(B), the efficiency of G4 DNA unwi ...
A Dnmt2-like protein mediates DNA methylation in
... Research article from Ambion. For controls, we synthesized double-stranded RNA from an EST clone (CK00414) (Kopczynski et al., 1998) of the CG11840 gene. Annealing of complementary RNA strands was verified by agarose gel electrophoresis. For microinjection, wild-type embryos were collected over 30 m ...
... Research article from Ambion. For controls, we synthesized double-stranded RNA from an EST clone (CK00414) (Kopczynski et al., 1998) of the CG11840 gene. Annealing of complementary RNA strands was verified by agarose gel electrophoresis. For microinjection, wild-type embryos were collected over 30 m ...
Site-Specific Integration of Transgenes in
... A1 was positive for both the 5# end and 3# end assays specific to RMCE (Fig. 2, A and B), negative for either the 5# end or the 3# end assays specific to the target (Fig. 2, C and D), and positive for a small excisionspecific band amplified by the full-length PCR (Fig. 2E). Since one copy of RMCE wa ...
... A1 was positive for both the 5# end and 3# end assays specific to RMCE (Fig. 2, A and B), negative for either the 5# end or the 3# end assays specific to the target (Fig. 2, C and D), and positive for a small excisionspecific band amplified by the full-length PCR (Fig. 2E). Since one copy of RMCE wa ...
Cloning of genes from genomic DNA: Part 3
... enzyme that leaves blunt ends as well and ligate the two together (blunt-ended cloning works, but is less efficient than cloning sticky ends). When PCR was first used to clone fragments, many people tried this and had very little success. It was discovered that the PCR enzyme, Taq polymerase, would ...
... enzyme that leaves blunt ends as well and ligate the two together (blunt-ended cloning works, but is less efficient than cloning sticky ends). When PCR was first used to clone fragments, many people tried this and had very little success. It was discovered that the PCR enzyme, Taq polymerase, would ...
Document
... *DNA fingerprinting. The use of restriction enzymes to measure the genetic variations among individuals. DNA haplotype. A pattern of DNA polymorphisms. DNA hybridization. The formation of a double-stranded nucleic acid molecule from two separate strands; also applies to a molecular technique that u ...
... *DNA fingerprinting. The use of restriction enzymes to measure the genetic variations among individuals. DNA haplotype. A pattern of DNA polymorphisms. DNA hybridization. The formation of a double-stranded nucleic acid molecule from two separate strands; also applies to a molecular technique that u ...
Relationship between Folding and Function in a Sequence
... salt concentration as binding buffer. CD experiments were conducted at 4 °C on an Aviv Model 202 spectrometer using a 2 mm path length cell. Samples were scanned from 200 to 250 nm, with a 6 s averaging time and 1 nm step size. RESULTS QuantitatiVe Analysis of DNA Affinity among p007 Variants. To st ...
... salt concentration as binding buffer. CD experiments were conducted at 4 °C on an Aviv Model 202 spectrometer using a 2 mm path length cell. Samples were scanned from 200 to 250 nm, with a 6 s averaging time and 1 nm step size. RESULTS QuantitatiVe Analysis of DNA Affinity among p007 Variants. To st ...
Molecular analysis of putative genetic factors affecting BSE
... the laboratory. Some of the samples were 6 years old and in very limited supply when used so the purity could not be improved. Given this variability between samples the study was continued by analysing individual samples, rather than pools. This was considerably more labour intensive, but increased ...
... the laboratory. Some of the samples were 6 years old and in very limited supply when used so the purity could not be improved. Given this variability between samples the study was continued by analysing individual samples, rather than pools. This was considerably more labour intensive, but increased ...
Book 12 Chapter 34 - From The Mountain Prophecies
... schemes to torture me, and or to get control of me, and/or the DNA any way they can! Daily, they are driving these things into the spaces between the teeth, into the roots of the teeth, into the nerves of the teeth and onto the upper parts of the teeth, jaws, nose and face! My Dear Ones, I have witn ...
... schemes to torture me, and or to get control of me, and/or the DNA any way they can! Daily, they are driving these things into the spaces between the teeth, into the roots of the teeth, into the nerves of the teeth and onto the upper parts of the teeth, jaws, nose and face! My Dear Ones, I have witn ...
Agarose Gel Electrophoresis
... large DNA fragments, as shown in Figure 2.5A.2B. For separating large DNA molecules, it is best to run gels at both low agarose concentrations and low applied voltages (∼1 V/cm, 0.5% agarose). Electrophoresis buffers. The two most widely used electrophoresis buffers are Tris/ acetate (TAE) and Tris/ ...
... large DNA fragments, as shown in Figure 2.5A.2B. For separating large DNA molecules, it is best to run gels at both low agarose concentrations and low applied voltages (∼1 V/cm, 0.5% agarose). Electrophoresis buffers. The two most widely used electrophoresis buffers are Tris/ acetate (TAE) and Tris/ ...
Biology Ch. 13
... Chapter 13 Genetics and Biotechnology Section 1: Applied Genetics Section 2: DNA Technology Section 3: The Human Genome ...
... Chapter 13 Genetics and Biotechnology Section 1: Applied Genetics Section 2: DNA Technology Section 3: The Human Genome ...
STRUCTURE AND DIAGNOSTIC APPLICATIONS OF DNA
... • Primase: RNA Polymerase that synthesizes the Primer for DNA replication; • Primase synthesizes RNA directly on the singlestranded DNA template because it does not require a Primer to begin synthesis; • New Deoxy-nucleotides are added to 3’ end Primer, ...
... • Primase: RNA Polymerase that synthesizes the Primer for DNA replication; • Primase synthesizes RNA directly on the singlestranded DNA template because it does not require a Primer to begin synthesis; • New Deoxy-nucleotides are added to 3’ end Primer, ...
GENECLEAN® Kit
... DNA. The fact that DNA binds in high salt and elutes in low salt makes this method especially useful as a purification procedure. Since the DNA is eluted with either water or a low salt buffer, it can be used immediately in subsequent reactions without precipitation or other further manipulation. Th ...
... DNA. The fact that DNA binds in high salt and elutes in low salt makes this method especially useful as a purification procedure. Since the DNA is eluted with either water or a low salt buffer, it can be used immediately in subsequent reactions without precipitation or other further manipulation. Th ...
Biology Review
... the two DNA strands. The helix is “right handed” curving up to the right. The two strands are held together by hydrogen bonds (dotted lines) between the nitrogenous bases which are paired in the interior of the double helix. B) For clarity, the two strands of DNA are shown untwisted in this partial ...
... the two DNA strands. The helix is “right handed” curving up to the right. The two strands are held together by hydrogen bonds (dotted lines) between the nitrogenous bases which are paired in the interior of the double helix. B) For clarity, the two strands of DNA are shown untwisted in this partial ...
16 System and a 10X Primer Pair Mix Stored in TE
... dNTP and 1.6mg/ml BSA) and added MgCl2 to the reactions separately to a final concentration of 1mM, 1.25mM, 1.5mM, 1.75mM or 2mM. For both primer formulations, the reactions produced similar results (Figure 1). The best balance between loci was achieved with 1.5mM MgCl2. Increasing MgCl2 concentrati ...
... dNTP and 1.6mg/ml BSA) and added MgCl2 to the reactions separately to a final concentration of 1mM, 1.25mM, 1.5mM, 1.75mM or 2mM. For both primer formulations, the reactions produced similar results (Figure 1). The best balance between loci was achieved with 1.5mM MgCl2. Increasing MgCl2 concentrati ...
Screening of RYR1 genotypes in swine population by a rapid and
... assay design are crucial points that can increase the amplitude of the profile difference and make sequence discrimination easier. However, the primers used for HRM must generate short amplicons. According to the manufacturer's recommendation the best results can be obtained with amplicons up to 300 ...
... assay design are crucial points that can increase the amplitude of the profile difference and make sequence discrimination easier. However, the primers used for HRM must generate short amplicons. According to the manufacturer's recommendation the best results can be obtained with amplicons up to 300 ...
DNA profiling
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DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.