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DNA PPT
DNA PPT

...  Enzymes help make new strands of DNA  One enzyme called helicase “unzips” the DNA, separating the base pairs  DNA polymerase adds new bases to pair up with the template  This enzyme also proofreads to make sure everything matches ...
DNA PPT
DNA PPT

Answers - MrsPalffysAPBio2013
Answers - MrsPalffysAPBio2013

... 3’ end of an existing nucleic acid. •First, an RNA primer of ~10 nucleotides is made by primase so that DNA polymerase has something to attach to & can begin constructing a new DNA strand •Therefore, at a replication fork, the complementary strands of DNA are not created at the same rate! •The leadi ...
DNA CODES…
DNA CODES…

... has, only 1% of it actually codes for proteins. Scientists are still trying to determine what the rest of the DNA is used for. A section of DNA that codes for a protein is called a gene. DNA is found in the nucleus of a eukaryotic cell. Genes need to be TRANSCRIBED into an mRNA molecule. DNA will un ...
Introduction to DNA
Introduction to DNA

...  Make your DNA and RNA using the pipe cleaner given and the colored beads.  Show me your RNA strand. If it is correct then turn into me your RNA sequences written down on paper. Return the beads and pipe cleaners  DNA sequence one ...
DNA RESTRICTION ANALYSIS
DNA RESTRICTION ANALYSIS

... electrophoresis box, so that comb is at negative (BLACK) cathode end. The - charged DNA fragments will migrate towards the + anode end. 7. Fill box with TAE buffer, to level that just covers entire surface of gel by about 2mm. 8. Make certain that sample wells left by comb are completely submerged b ...
RNA
RNA

Whole genome sequencing - Center for Biological Sequence Analysis
Whole genome sequencing - Center for Biological Sequence Analysis

SNP Discovery Services - Sanger Sequencing
SNP Discovery Services - Sanger Sequencing

... Sending a sample for a de novo sequencing project: The concentration of plasmid DNA required is between 100 and 500 ng/µl. The DNA must be of good quality in order to ensure that the sequencing reactions work adequately. The required quantity of plasmid DNA for a project is approximately 2 µl or 4 µ ...
Exam 2
Exam 2

... 4) You have isolated a virus with both DNA and RNA in it. Briefly describe one experiment that you would do to determine whether DNA or the RNA was the genetic material? Answer #1: Selectively labeled the virus DNA with radioactive thymine (or deoxyribose) in tube#1 and label the virus RNA with rad ...
DNA PPT
DNA PPT

DNA Isolation - Flinn Scientific
DNA Isolation - Flinn Scientific

... The steps in this laboratory procedure teach a great deal about the properties of cells, cell membranes, and deoxyribonucleic acid (DNA) itself. The collection of cheek cells from the inside of the mouth highlights the nature of body tissue. Dead cells are continually being sloughed off on both the ...
Making Recombinant DNA
Making Recombinant DNA

... the DNA/RNA of the invader in small fragments; consequently the viral DNA is not able to launch the reverse transcriptase in order to use the bacteria’s own enzymatic machinery to produce more viruses. So far, many different restriction enzymes have been identified. Each restriction enzyme “cuts” at ...
13.2 abbreviated Interactive Text
13.2 abbreviated Interactive Text

... offspring have to mature before the traits become obvious. Sometimes it takes several generations before the desired trait becomes common in the population. There is a faster and more reliable way to increase the frequency of a desired allele in a population. It is called genetic engineering. In gen ...
Introduction to some basic features of genetic information
Introduction to some basic features of genetic information

... seaweeds, fungi, plant, animals) that can have many different cell and tissue types.1 DNA contains the genetic ‘code’ of information that makes each species unique. Smaller variations in the DNA can lead to minor differences among individuals of the same species. The combination of specific DNA comp ...
DNA
DNA

... • In many-celled organisms like you, each cell uses only some of the thousands of genes that it has to make proteins. • Each cell uses only the genes that direct the making of proteins that it needs. • For example, muscle proteins are made in muscle cells but not in nerve cells. ...
Chapter 22 Lecture PowerPoint - McGraw Hill Higher Education
Chapter 22 Lecture PowerPoint - McGraw Hill Higher Education

... – When tail finds homologous region, nick occurs in in D-looped DNA – Nick allows RecA and ss-break create a new tail that can pair with gap in the other DNA ...
Chromosomes in prokaryotes
Chromosomes in prokaryotes

... not condensed into chromosomes as in eukaryotes. Structure in sequences There is a very high proportion of coding DNA and an absence of repeats in bacteria genome. Bacteria typically have a single origin of replication. The genes in prokaryotes are often organized in operons, and do not contain intr ...
Genomes and Chromosomes - Microbiology and Molecular
Genomes and Chromosomes - Microbiology and Molecular

... - High-copy-number plasmids segregate randomly to daughter cells. Plasmids are advantageous under certain conditions: - Resistance to antibiotics and toxic metals - Pathogenesis - Symbiosis Plasmids can also be transferred between cells. ...
Unit 6. Week 1. DNA and RNA (2)
Unit 6. Week 1. DNA and RNA (2)

How many tetrads are there in metaphase I of
How many tetrads are there in metaphase I of

... from each parental strand; ligase is used to connect these short segments of both daughter strands. B. Two DNA polymerase molecules act to synthesize daughter DNA strands: one via a long continuous strand that moves in the same direction as the helicase, and a second polymerase synthesizes short seg ...
Exploring DNA Structures
Exploring DNA Structures

... Background Information: DNA is the basic material that contains the information that is responsible for the way all living organisms physically look and instruction on how to carry out the activities of the cell. We are going to explore the different parts of DNA. READ THIS BEFORE MOVING ON: Before ...
Document
Document

... Replication Models • Conservative- would leave the original strand intact and copy it. • Dispersive-would produce two DNA molecule with sections of both old and new along each strand. • Semiconservative –would produce DNA molecule with both one old strand and one new strand. ...
Document
Document

DNA - Guilford, CT
DNA - Guilford, CT

... DNA is too simple a molecule to be the genetic material. By lysing S cells, separating the contents- lipids, proteins, polysaccharides, nucleic acids (DNA,RNA) and testing each fraction to see if it could transform living R cells into S cells. Only when R cells were treated with the nucleic acids fr ...
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DNA profiling



DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.
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