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DNA - Mr. Johnston's Biology Site
DNA - Mr. Johnston's Biology Site

... mRNA is read to make proteins  mRNA attaches to the ribosome  Ribosome begins reading codons Codon = group of 3 bases that codes for an amino acid  tRNA brings the correct amino acid to the ribosome  Ribosome assembles protein (polypeptide) by combining amino acids using peptide bonds ...
AP03–DNA is Everywhere - Science from Scientists
AP03–DNA is Everywhere - Science from Scientists

... Cells – the fundamental building blocks of life; some life such as algae and bacteria are unicellular, and others are multicellular like humans and trees; multicellular life contains many differentiated cells with specialized functions, but all cells making up an organism contain the same DNA Cell m ...
Chapter 3 Methods in Molecular Biology and Genetic
Chapter 3 Methods in Molecular Biology and Genetic

... •  Gene expression array are used to detect the level of all the expressed genes in an experimental sample. •  SNP arrays permit genome-wide genotyping of single nucleotide polymorphisms. =>use allele-specific oligonucledtide probe •  Array comparative genome hybridization (arrayCGH) allows the dete ...
Lecture 3
Lecture 3

... • In 1953, James Watson and Francis Crick proposed that DNA is a double-stranded molecule twisted into a helix (the double helix structure) • Each spiraling strand, comprised of a sugarphosphate backbone and attached bases, is connected to a complementary strand by non-covalent hydrogen bonding bet ...
DNA Scientists
DNA Scientists

...  Studied numerous organisms to find chromosomes but ultimately had the most success with grasshopper sperm cells.  He expanded on Mendel’s work and found that chromosomes are what carry genetic information; they are found in a pair in body cells and one set is given to offspring from each parent t ...
1. (i) Purines pair with pyrimidines / adenine and thymine always
1. (i) Purines pair with pyrimidines / adenine and thymine always

... Gametes are haploid (or concept explained) / each gamete will contain 7 units; ...
Chapter 11 Jeopardy Review
Chapter 11 Jeopardy Review

... •tRNA attaches to amino acids from the cytoplasm and brings them to the ribosome •DNA replicates itself in the nucleus •The single, unzipped DNA strand is transcribed onto the mRNA •DNA unzips •tRNA anticodons bind with the codons on the ribosome •Neighboring amino acids bind together •Long chains o ...
Basic Genetics- Powerpoint
Basic Genetics- Powerpoint

... Breeding continued: ...
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... DNA floats freely in the cytoplasm of prokaryotic cells like bacteria. DNA is protected in the nucleus of _eukaryotic cells like “you”. ...
Sample exam questions: DNA, transcription, and translation
Sample exam questions: DNA, transcription, and translation

... hence the capital letters, but all newly synthesized DNA in the daughter cells will have normal N (lower case letters) 5’ aaaggg . . . . . . . . x . . . . . . . ccctttggg 3’ 3’ TTTCCC . . . . . . . . X . . . . . . . GGGAAACCC 5’ That cell divides to make two daughter cells, which in turn divide to m ...
BI0034
BI0034

... 6) Determination of gene patterns with a given minimal length and presence in at least q (quorum) different genomes providing systematic and heuristic search strategies, 7) Clustering and visualisation of gene pattern instances concerning gene order and direction together with their environmental pa ...
transcription-and-translation-assessment-statements-2016
transcription-and-translation-assessment-statements-2016

... 7.3.1 Outline the structure of ribosomes, including protein and RNA composition, large and small subunits, three tRNA binding sites(explain their roles) and mRNA binding sites 7.3.2 List and describe the 4 steps in translation(initiation, elongation, translocation and termination 7.3.3 State that tr ...
DNA Replication - Blue Valley Schools
DNA Replication - Blue Valley Schools

... bonding of A with T and G with C. • This pattern is constant for all organisms. • The sequence of bases in a nucleotide strand is different from one species to the next. ...
Human Identity Testing
Human Identity Testing

... us consider those repeats with a frequency greater than 1% in the population. They are: 6, 7, 8, 9, 9.3, and 10 repeats. The first thing that you may wonder about is this 9.3 business. What is that? Nature is seldom obliging to logic and neatness and this is an example. The notation 9.3 means that t ...
Biotechnology and Genetic Engineering
Biotechnology and Genetic Engineering

... •7. DNA which is negative is picked up by current and carried towards the positive end of the apparatus because opposites attract. •8. The different size DNA fragments travel different distances. The largest fragments move the least distance and the shorter fragments move the most. •9. A dye is add ...
Beginning to crack the code of `junk DNA`
Beginning to crack the code of `junk DNA`

... in Turkey. In the early 1920s, he came to the United States and became a rug merchant. Medicine would have to wait until the next generation. But Kazazian switched from medicine to genetics in the 1960s, inspired by the new vistas of knowledge the field was opening up. In 1969, he joined the faculty ...
DNA and Molecular Genetics
DNA and Molecular Genetics

... proteins (in ribosomes in cytoplasm and on rough ER) from specific nucleotide sequences (in DNA of genes in nucleus) • Need a way to get information from nucleus to ribosomes  done with RNA • Actually three types of RNA that all help to read the DNA code and produce proteins ...


... * DNA copy gain/loss within a known clinically significant gene region of 50 Kb or greater. * DNA copy number loss of >200 kb or gain >500 kb outside known clinically significant regions with at least one OMIM annotated gene or within a region of clear clinical significance. * UPD testing is recomme ...
Name: Period _______ Date FINAL EXAM STUDY GUIDE G
Name: Period _______ Date FINAL EXAM STUDY GUIDE G

... Karyotype (know how to read and analyze): Be able to analyze blood types as co-dominant and multiple alleles Be able to set up and complete a dihybrid Punnett square and analyze probability ratios for genotype and phenotype. Homologous/Non-homologous chromosomes Mutations of gametes: Deletion, inver ...
Real-time PCR
Real-time PCR

... Less commonly used now Fluorophore ...
Sequencing the Human Genome
Sequencing the Human Genome

... 1988: NAS report appears, stressing multidisciplinary participation of labs across the country. The House Energy and Commerce Committee decides that the government should fund such an effort. 1990: Joint public effort launched, at an estimated cost of $3 billion, by the International Human Genome Map ...
Virtual Mark Recapture Lab
Virtual Mark Recapture Lab

... 4. Which of the following represents the correct order of steps in DNA fingerprinting? a. DNA isolationrestriction enzyme digestiongel electrophoresis b. Restriction enzyme digestiongel electrophoresisDNA isolation c. DNA isolationgel electrophoresisrestriction enzyme digestion d. None of the ...
DNA Technology: The Future Is Now
DNA Technology: The Future Is Now

... only began in April of 1995. In only 5 years, the English have created a database of nearly 1 million profiles of suspects and convicted offenders. Using this database, over 72,000 suspects have been linked to crimes and another 10,000 cases were linked to other similar crimes, even though the suspe ...
DNA and Heredity
DNA and Heredity

... very likely use a molecule with a similar function. ...
Module 5 Gene Mutations
Module 5 Gene Mutations

... Dominant Mutations • Dominant Mutations appear in the immediate offspring if it is found in at least one parent. • Examples of dominant gene diseases include polydactyly, achondroplasia, huntington’s Chorea, and retinoblastoma ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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