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Identification of a factor IX point mutation using SSCP analysis and
Identification of a factor IX point mutation using SSCP analysis and

... Hemophilia B is an X-linked recessive disorder characterized by reduced or functionally defective factor IX. Severity of the disease depends on the functional significance of the genetic alteration. We report here a C - T transition at nucleotide 20518 (numbering by (1)) of the factor IX gene of an ...
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Proposal for 431 531 - Oregon State University
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... In 1986, I was able to do a reasonable job of presenting the principles of plant genetics to an undergraduate audience with little or no background in the subject. I was able to offer sufficient additional material to justify offering the course for graduate credit, particularly for students new to ...
Proposal for 431 531 - Oregon State University
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RNA - TeacherWeb
RNA - TeacherWeb

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DNA extraction from cheek cells protocol I mailed to you
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Development of triplet repeat primed PCR (TP

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... the best known debate on these centers on corn. A recent French study showed severe kidney and liver abnormalities in rats that were fed this corn for up to 2 years.1 Immediately afterward, Russia banned the use of this seed and the corn it produces. Because other studies have not confirmed this fin ...


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Basic molecular genetics for epidemiologists
Basic molecular genetics for epidemiologists

... motif 1–6 basepairs long. Microsatellites tend to be polymorphic in their number of repetitions because of a high mutation rate. DNA polymerases tend to “slip” when copying microsatellite tracts, adding or subtracting repeat units. Given their high polymorphism, microsatellites are widely used in ma ...
DNA - K.T. Leung
DNA - K.T. Leung

... 3.4Å between nucleotide base pairs. 34Å / 360° turn. 10 nucleotide base pairs / 360° turn. The process that forms double helix is called DNA hybridization. The order or sequence of these bases along the chain forms the genetic code. ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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