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The Nobel Prize in Medicine
The Nobel Prize in Medicine

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Chapter 11: DNA: The Molecule of Heredity
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Gel Electrophoresis of DNA
Gel Electrophoresis of DNA

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Slide 1
Slide 1

... rate (μ) is equal to the rate of substitution for neutral alleles (K). Thus, homologous stretches of nonfunctional DNA, on which selection does not operate and which evolve by random genetic drift, can be compared between two species to calculate the amount of sequence divergence. If the generation ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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