Chapter 5 - FIU Faculty Websites
... mammal genome ~ 3-4 billion base pairs of DNA one gene ~ 10 kbp It would be unpractical to isolate 1 gene Preparation of genomic DNA library Isolating total DNA – Aplying shotgun cloning Digesting total DNA into fragments of suitable size (the fragments do not corresponds to the individual genes) In ...
... mammal genome ~ 3-4 billion base pairs of DNA one gene ~ 10 kbp It would be unpractical to isolate 1 gene Preparation of genomic DNA library Isolating total DNA – Aplying shotgun cloning Digesting total DNA into fragments of suitable size (the fragments do not corresponds to the individual genes) In ...
Advancing Science with DNA Sequence
... Metagenomic data are noisy • Definition of high quality genome sequence: an example of “finished” JGI genomes - each base is covered by at least two Sanger reads in each direction with a quality of at least Q20 • Definition of “ high quality” metagenome? Too many variables: species composition/abu ...
... Metagenomic data are noisy • Definition of high quality genome sequence: an example of “finished” JGI genomes - each base is covered by at least two Sanger reads in each direction with a quality of at least Q20 • Definition of “ high quality” metagenome? Too many variables: species composition/abu ...
Transcription and Translation of DNA
... Multiple translation is achieved by several ribosomes being attached to the ribosome and translating at the same time In this way many copies of the polypeptide are made ...
... Multiple translation is achieved by several ribosomes being attached to the ribosome and translating at the same time In this way many copies of the polypeptide are made ...
DNA Sequence Analysis Using Boolean Algebra
... National Center for Biotechnology Information, hosts more than 25 million sequence records comprising more than 32 billion nucleotides. The dynamic programming algorithm for alignment between two DNA sequences proposed by Smith and Waterman, Needleman-Wunsch is a very well known and versatile algori ...
... National Center for Biotechnology Information, hosts more than 25 million sequence records comprising more than 32 billion nucleotides. The dynamic programming algorithm for alignment between two DNA sequences proposed by Smith and Waterman, Needleman-Wunsch is a very well known and versatile algori ...
Notes: DNA Fingerprinting, C.7
... DNA analysis allows even a small sample of tissue to be identified with a single individual. DNA contains, in non-coding regions called junk DNA, many repeated sequences that vary in number between individuals. These differences between individuals can be used to produce a DNA fingerprint for an ind ...
... DNA analysis allows even a small sample of tissue to be identified with a single individual. DNA contains, in non-coding regions called junk DNA, many repeated sequences that vary in number between individuals. These differences between individuals can be used to produce a DNA fingerprint for an ind ...
PPT
... Some “chemical THING” was transferred from the heat-killed cells of the S strain into the live cells of the R strain, making the R strain deadly! This is called Transformation. ...
... Some “chemical THING” was transferred from the heat-killed cells of the S strain into the live cells of the R strain, making the R strain deadly! This is called Transformation. ...
Chapter 4 - Version B
... 26. The percentage composition of a nucleic acid molecule found in bacterial cells is 32.3% adenine 30.7% thymine 19.1% cytosine 17.9% guanine The molecule is most likely to be a. double-stranded DNA. b. mitochondrial DNA. c. messenger RNA. ...
... 26. The percentage composition of a nucleic acid molecule found in bacterial cells is 32.3% adenine 30.7% thymine 19.1% cytosine 17.9% guanine The molecule is most likely to be a. double-stranded DNA. b. mitochondrial DNA. c. messenger RNA. ...
Chapter 4 - Version A
... 17. During DNA replication, one of the new strands of DNA is synthesized continuously, while the other is synthesized as a number of separate fragments of DNA that are subsequently linked by DNA ligase. This is because a. replication starts at many points on the chromosome b. RNA primers only anneal ...
... 17. During DNA replication, one of the new strands of DNA is synthesized continuously, while the other is synthesized as a number of separate fragments of DNA that are subsequently linked by DNA ligase. This is because a. replication starts at many points on the chromosome b. RNA primers only anneal ...
Powerpoint
... Single-strand binding proteins Primase DNA polymerase DNA ligase Okazaki fragments ...
... Single-strand binding proteins Primase DNA polymerase DNA ligase Okazaki fragments ...
transcription, translation
... molecule to be easily transcribed. Whys is this important for genetic information? 3. Whys is RNA important to the cell? How does an mRNA molecule carry information from DNA? 4. If DNA strand read AAC GTC GCG TAC, what would the mRNA strand be? ...
... molecule to be easily transcribed. Whys is this important for genetic information? 3. Whys is RNA important to the cell? How does an mRNA molecule carry information from DNA? 4. If DNA strand read AAC GTC GCG TAC, what would the mRNA strand be? ...
central_dogma_(short_revised)
... RNA is fantastic It’s _____________ Ribonucleic acid two On the sugar ribose it has ___ hydroxyl It’s negatively charged on the Phosphate ...
... RNA is fantastic It’s _____________ Ribonucleic acid two On the sugar ribose it has ___ hydroxyl It’s negatively charged on the Phosphate ...
GPS standards
... SFS3. Students will analyze the use of toxicology, serology, and DNA technology in forensic investigations. a. Classify toxins and their effects on the body. b. Compare the effects of alcohol on blood alcohol levels with regard to gender, and according to the law. c. Evaluate forensic techniques use ...
... SFS3. Students will analyze the use of toxicology, serology, and DNA technology in forensic investigations. a. Classify toxins and their effects on the body. b. Compare the effects of alcohol on blood alcohol levels with regard to gender, and according to the law. c. Evaluate forensic techniques use ...
No Slide Title
... • Watson-Crick DNA model is of B-DNA, which is believed to be biologically significant form • A-DNA is slightly more compact than B-DNA • C-DNA, D-DNA, and E-DNA also right-handed forms of DNA, are less compact than B-DNA • Z-DNA forms a left-handed double helix ...
... • Watson-Crick DNA model is of B-DNA, which is believed to be biologically significant form • A-DNA is slightly more compact than B-DNA • C-DNA, D-DNA, and E-DNA also right-handed forms of DNA, are less compact than B-DNA • Z-DNA forms a left-handed double helix ...
I. Introduction: Definitions and mutation rates
... have very serious consquences, as in the case of sickle-cell anemia, mild consequences as in the case of hemoglobin C (a different amino acid substitution in position 6 of beta-globin) or no phenotype as in the case of two known amino acid substitutions at position 7 of beta-globin. Finally, base s ...
... have very serious consquences, as in the case of sickle-cell anemia, mild consequences as in the case of hemoglobin C (a different amino acid substitution in position 6 of beta-globin) or no phenotype as in the case of two known amino acid substitutions at position 7 of beta-globin. Finally, base s ...
Bio-CS 251 syllabus `06 - Gettysburg College Computer Science
... discovery and analysis, and will study genome structure and genome evolution. The student will also employ in silico DNA microarray methods for measuring global gene expression, and will use proteomic tools for in silico analysis of the products encoded by genes that are discovered in the course of ...
... discovery and analysis, and will study genome structure and genome evolution. The student will also employ in silico DNA microarray methods for measuring global gene expression, and will use proteomic tools for in silico analysis of the products encoded by genes that are discovered in the course of ...
Document
... c. the resulting clones are genetically identical to the donor parent regardless of the adult cell type used as a donor d. differentiated adult cells must all have the same DNA if clones result from nuclear transplantation regardless of the donor cell type ...
... c. the resulting clones are genetically identical to the donor parent regardless of the adult cell type used as a donor d. differentiated adult cells must all have the same DNA if clones result from nuclear transplantation regardless of the donor cell type ...
1. What are the four nitrogenous bases found in DNA? 1. Where in
... 6. There is only one sugarphosphate “backbone” in RNA meaning it is single stranded. ...
... 6. There is only one sugarphosphate “backbone” in RNA meaning it is single stranded. ...
Searching for microsatellite mutations in coding regions in
... Received 25 September 2000; revised 28 December 2000; accepted 3 January 2001 ...
... Received 25 September 2000; revised 28 December 2000; accepted 3 January 2001 ...
Whole-Genome Chromatin IP Sequencing (ChIP-Seq)
... sequences. The ChIP process enriches specific crosslinked DNAprotein complexes using an antibody against a protein of interest (Figure 1). Oligonucleotide adapters are then added to the small stretches of DNA that were bound to the protein of interest to enable massively parallel sequencing. After s ...
... sequences. The ChIP process enriches specific crosslinked DNAprotein complexes using an antibody against a protein of interest (Figure 1). Oligonucleotide adapters are then added to the small stretches of DNA that were bound to the protein of interest to enable massively parallel sequencing. After s ...
What is the Structure of DNA?
... The new chromosome has a bit of single stranded DNA at each end (on the lagging strand) — this region is cut off. Eukaryote chromosomes have repetitive sequences at the ends called telomeres. ...
... The new chromosome has a bit of single stranded DNA at each end (on the lagging strand) — this region is cut off. Eukaryote chromosomes have repetitive sequences at the ends called telomeres. ...
Slide
... inhibits the activity of the enzyme RNA polymerase • As a result, the affected gene cannot be transcribed – it is effectively ...
... inhibits the activity of the enzyme RNA polymerase • As a result, the affected gene cannot be transcribed – it is effectively ...
MUTATIONS
... genes that provide the raw material for evolution. Most mutations have no effect on the organism, especially among the eukaryotes, because a large portion of the DNA is not in genes and thus does not affect the organism’s phenotype. Only a small percentage of mutations causes a visible but non-l ...
... genes that provide the raw material for evolution. Most mutations have no effect on the organism, especially among the eukaryotes, because a large portion of the DNA is not in genes and thus does not affect the organism’s phenotype. Only a small percentage of mutations causes a visible but non-l ...
DNA strucutre and replication
... Discovering the structure of DNA • DNA = Deoxyribose nucleic acid • Made out of sugars (deoxyribose), phosphates and nitrogen bases ...
... Discovering the structure of DNA • DNA = Deoxyribose nucleic acid • Made out of sugars (deoxyribose), phosphates and nitrogen bases ...
i. dna structure and functions
... adenine, guanine, cytosine, and uracil (U) as the 4 bases (U replaces T) B. DNA Functions 1. DNA, as the hereditary material,: a) Replicate with high accuracy and be transmitted from one generation to the next generation; 2. DNA Controls the Cell a) One gene one protein b) A gene is a section of a ...
... adenine, guanine, cytosine, and uracil (U) as the 4 bases (U replaces T) B. DNA Functions 1. DNA, as the hereditary material,: a) Replicate with high accuracy and be transmitted from one generation to the next generation; 2. DNA Controls the Cell a) One gene one protein b) A gene is a section of a ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.