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DNA - Corner Canyon Honors Biology
DNA - Corner Canyon Honors Biology

... The Process of Protein Synthesis • The first codon is usually A-U-G, METHIONINE, which starts the production of a new protein • Following the start codon, the remaining codons call for amino acids in the order in which they appear on the mRNA strand. ...
DNA Quiz
DNA Quiz

... d. bring amino acids from the cytoplasm to the ribosomes. ____ 14. (1 point) Which of the following events occurs directly after RNA polymerase recognizes the transcription start site of a gene? a. The polymerase strings amino acids into a polypeptide. b. Free-floating nucleotides pair up with expos ...
Molecular Biology - Charles River Laboratories
Molecular Biology - Charles River Laboratories

... Regulatory guidance recommends the inclusion of pharmacogenomic investigations during the drug or biological product development process, therefore you need a partner that can offer you the most advanced and reliable assays as well as the flexibility to tailor them to your specifications. Leveraging ...
Introduction
Introduction

... Consider a (0,1) matrix M, with rows indexed by clones and columns by probes, and position (i, j) is 1 if clone i contains probe j. The problem is to permute the columns so that the ones in each row are consecutive. A (0, 1) matrix has the k-consecutive ones property (k-C1P) if there exists a column ...
Reduced extension temperatures required for PCR amplification of
Reduced extension temperatures required for PCR amplification of

... denaturation of double-stranded DNA and annealing of oligonucleotide primers. At this temperature the thermostable polymerase replicates the DNA at an optimal rate that depends on the buffer and nature of the DNA template (1). Although the sizes of the fragments that can be amplified have been gener ...
Unit 3 notes
Unit 3 notes

... IV. Characteristics of DNA 1) Consists of two parallel helical or twisted chains, each made up of subunits called ___________________. 2) The deoxyribose and phosphate portions of the nucleotides are on the outside of the molecule forming the _________________ and the nitrogen bases are on the insid ...
DNA Molecular Structure
DNA Molecular Structure

... nucleotides into the language of amino acids • ribosomes - translate sequence of nucleotides into the sequence of amino acids – occur mainly in cytosol, on surface of rough ER, and nuclear envelope – consists of two granular subunits, large and small • each made of several rRNA and enzyme molecules ...
The Central Dogma of Molecular Biology
The Central Dogma of Molecular Biology

... protein. They are NOT transcribed * Exons – sequences in the DNA that are expressed or used to make mRNA and ultimately are used to make a protein ...
DNA str, Replication, Damage & Repair
DNA str, Replication, Damage & Repair

... Cytosine The bases form weak hydrogen bonds ...
3.1-BIO-GEN-dna.rna.transcription.translation
3.1-BIO-GEN-dna.rna.transcription.translation

... • Original DNA cannot pass through the nuclear membrane, so a smaller copy is made and transported ...
Plasmid w/ kanamycin resistance (pKAN)
Plasmid w/ kanamycin resistance (pKAN)

... Stage 1: Prepare your plasmids to be cut by restriction enzymes • Obtain the plasmids (pKAN and pAMP) P stands for plasmid pKAN = plasmid with antibiotic kanamycin resistance pAMP = plasmid with antibiotic ampicillin resistance ...
Document
Document

... phage protein coats remain in the supernatant while bacteria form a pellet ...
DNA
DNA

... – The transfer RNA(tRNA) molecules bring amino acids to the ribosome one at a time. – Each tRNA identifies the appropriate codon on the mRNA and add this amino acid to the growing protein chain. – The first tRNA is released and the ribosome moves one codon length along the message, allowing the next ...
UNIT REVIEW_DNA to Protein Synthesis
UNIT REVIEW_DNA to Protein Synthesis

... 21. DNA is called the "blueprint of life" because: DNA is called the blueprint of life because it contains the instructions needed for an organism to grow, develop, survive and reproduce. DNA does this by controlling protein synthesis. Proteins do most of the work in cells, and are the basic unit of ...
Solutions: Chapter 4 and 5 Review Sheet
Solutions: Chapter 4 and 5 Review Sheet

... 15.  DNA  replicates  in  a  semiconservative  manner  by  having  each  strand  of  the  double-­‐  stranded  parent  DNA  molecule   serve  as  a  template  for,  and  ultimately  end  up  as  part  of,  the  newly  formed  daughter ...
AFM image of DNA on mica with buffer
AFM image of DNA on mica with buffer

... Mica is bound to silicon using Mg2+ in a buffer solution instead of APTES mixed with the DNA that, like on silicon, sustains its biomolecules The purpose of DNA on mica is to see how it acts on a different surface from silicon ...
C - My CCSD
C - My CCSD

... – Thymine in DNA is replaced by Uracil (U) in mRNA – Therefore, the binding in mRNA goes as follows: ...
Plasmid w/ kanamycin resistance (pKAN)
Plasmid w/ kanamycin resistance (pKAN)

... Restriction enzyme: Bam HI (cuts @ bp 2095) 2333 bp restriction fragment ...
SAM Teachers Guide Nucleic Acids and Proteins - RI
SAM Teachers Guide Nucleic Acids and Proteins - RI

... and function. Atomic Structure introduces students to the positive and negative parts of atoms. Electrostatics explores attractions among charged particles. Intermolecular Attractions looks at the role of these attractions in protein folding and in the way nucleic acids act as a template for other n ...
TG_REV_NAP_r6 - RI
TG_REV_NAP_r6 - RI

... and function. Atomic Structure introduces students to the positive and negative parts of atoms. Electrostatics explores attractions among charged particles. Intermolecular Attractions looks at the role of these attractions in protein folding and in the way nucleic acids act as a template for other n ...
CHAPTER 11
CHAPTER 11

... important during translation for codon/anticodon recognition. It also allows RNA molecules to form secondary structures and to recognize each other. C20. Answer: G = 32%, C = 32%, A = 18%, T = 18%. C21. Answer: The number of bases per turn is different in an RNA double helix and a DNA double helix. ...
Student Name: Teacher
Student Name: Teacher

... Break down the nuclear membrane of cells, releasing DNA. Isolate DNA from proteins and sugars in the solution. Protect nucleic acids from enzymes used to destroy proteins. Sterilize the material from which DNA is to be extracted. ...
Biochemistry Lecture 21
Biochemistry Lecture 21

... DNA Polymerase – cont’d • Once DNA polymerase begins synth of DNA chain, can dissociate OR can continue along template adding more nucleotides to growing chain – Rate of synth DNA depends on ability of enz to continue w/out falling off ...
DNA structure and replication notes
DNA structure and replication notes

... opposite orientation of the strands is important in DNA replication.  DNA polymerases link DNA nucleotides to a growing daughter strand, only to the 3’ end of the strand, never to the 5’ end.  Thus, a daughter DNA strand can only grow in the 5’3’ direction. ...
Lesson 6. Nucleiotides
Lesson 6. Nucleiotides

... Unlike DNA, RNA molecules in primary structure are single stranded linear polymer containing nucleotides joined by phosphodiester linkage. The linkage of the ribonucleotides in RNA is 3’5’ phosphodiester link involve 3’-OH group of ribose and 5’-phosphate group of another ribonucleotide. In eukaryot ...
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DNA nanotechnology



DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.
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