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sample
sample

... themselves, then widen again, and so forth. My form is known as a doublehelical structure. It is composed of two intertwined, stringy, spaghetti-like strands called nucleic acid or DNA (deoxyribonucleic acid). ...
Ch 16 DNA structure and replication powerpoint
Ch 16 DNA structure and replication powerpoint

... phosphate, the other (3') a free hydroxyl (-OH) 2. double stranded molecule, the two strands are opposite, one is 3' to 5' and the other is 5' to 3' antiparallel. 3. New nucleotides can only be added to the 3' end 4. Continuous synthesis of both strands is not possible 5. DNA synthesis is continuous ...
Vocabulary: RNA and Protein Synthesis
Vocabulary: RNA and Protein Synthesis

... Transfer RNA – (tRNA) a strand of RNA that transfers amino acids to the growing end of a protein molecule during translation. ...
De novo sample preparation guidelines
De novo sample preparation guidelines

... heavily impact the final results. Protein contamination, nicks in the double-stranded DNA are major causes of experiments with data quality below expectation. Also good estimation of DNA integrity for any long-range application (mate-pairs, PacBio sequencing, Chromium linkedreads) is a critical poin ...
The Genetic Code for Certain Amino Acids
The Genetic Code for Certain Amino Acids

... pyrimidine and one purine, and each base bonds exclusively with only one other base; adenine with thymine, and cytosine with quanine. The monomer (individual unit) of a nucleic acid is called a nucleotide; this is composed of a phosphate, sugar and one base. The nucleotides are referred to by the ba ...
Chapter 4
Chapter 4

... Transcription begins near promoter sites ...
Name: Biochemistry 465 Hour exam II Spring 2006
Name: Biochemistry 465 Hour exam II Spring 2006

... A) associates with the promoter before binding core enzyme. B) combines with the core enzyme to confer specific binding to a promoter. C) is inseparable from the core enzyme. D) is required for termination of an RNA chain. E) will catalyze synthesis of RNA from both DNA template strands in the absen ...
Molecular Genetics Notes
Molecular Genetics Notes

... Single stranded mRNA moves OUTSIDE the nucleus to the ribosome mRNA attaches to the RIBOSOME, which reads the codon tRNA’s anticodon matches to the codon and brings Amino Acids to the ribosomes ...
Recap of 8.1 and 8.2
Recap of 8.1 and 8.2

... So how is the structure of DNA linked to its function? 1. DNA is very stable: It passes from generation to generation without changing. 2. The two strands are linked only by hydrogen bonds: During DNA replication and protein synthesis, the strands can separate easily. 3. It’s a huge molecule: It ca ...
Ch. 11 Powerpoint - Plain Local Schools
Ch. 11 Powerpoint - Plain Local Schools

... The transcript of these genes is the pre-mRNA (precursor-mRNA). The pre-mRNA is processed in the nucleus to remove the introns and splice the exons together into a translatable mRNA. That mRNA exits the nucleus and is translated in the ...
DNA - The Double Helix
DNA - The Double Helix

... for Ribonucleic Acid. Color the mRNA as you did the DNA, except: Color the ribose a DARKER BLUE, and the uracil brown. The Blueprint of Life Every cell in your body has the same "blueprint" or the same DNA. Like the blueprints of a house tell the builders how to construct a house, the DNA "blueprin ...
ch10 GN
ch10 GN

... • RNA ______________________________attaches to a special place (certain base sequence called ____________________________) on the DNA molecule and moves along the strand, unwinding and separating the strands. • The RNA polymerase then begins reading and copying the DNA as it goes along. “zips up” a ...
Name:
Name:

... to one another and to atoms of other elements (especially H, O, N). Compounds made by cells and containing carbon are known as organic compounds. Carbon can form four covalent bonds with other carbons or other types of atoms. Cells make a huge number of large molecules from a small set of small mole ...
Module 14 Nucleic Acids Lecture 36 Nucleic Acids I
Module 14 Nucleic Acids Lecture 36 Nucleic Acids I

... NPTEL – Biotechnology – Cell Biology ...
Chapter 12 DNA Structure and Function
Chapter 12 DNA Structure and Function

... stops, and another polymerase binds farther down the chain. • This process creates several fragments, called Okazaki Fragments, that are bound together by DNA ligase. ...
Notes Protein Synthesis
Notes Protein Synthesis

...  DNA separates b/w nitrogen bases  Hydrogen bonds are broken  Each strand becomes a “template” where replication occurs  DNA replication results in two exact copies of the cells DNA 1 is the original DNA strand the other is a new strand  Provides information to RNA for protein synthesis ...
Chapter 10 - Richsingiser.com
Chapter 10 - Richsingiser.com

... The Base Pairs Postulated by Watson ...
CHAPTER 2 The Chemistry of Living Things
CHAPTER 2 The Chemistry of Living Things

... 5. Blotting. An radioactive probe. fragments are alkaline solution Incubate the nylon treated with an wicks up into membrane with a solution alkaline blotting paper, containing labeled probe solution to carrying DNA from DNA. The radioactive make them gel onto nylon filter, probe base pairs to the s ...
DNA REVIEW Name
DNA REVIEW Name

... When does replication of chromosomes take place in the cell cycle? S stage of interphase 2. Know these people and their contributions: James Watson and Francis Crick— made the 1st correct model of the DNA molecule Rosalind Franklin and Maurice Wilkins—Worked with X-ray crystallography and created X- ...
Prescott`s Microbiology, 9th Edition Chapter 16 –Mechanisms of
Prescott`s Microbiology, 9th Edition Chapter 16 –Mechanisms of

... If non homologous DNA was taken into the cell, it would not be able to align itself with a homologous region on the bacterial chromosome, and thus would not be integrated. The DNA could be degraded, but if not, could still only be passed to one daughter cell during cell division, and would thus be d ...
Deoxyribose nucleic acid
Deoxyribose nucleic acid

... makes sure there are no mistakes 4. DNA helicase zips the half old/ half new strands of DNA back up and winds it back into a helix ...
How-to-use guide for the Nanodrop Spectrophotometer
How-to-use guide for the Nanodrop Spectrophotometer

... The ratio of absorbance at 260 nm and 280 nm is used to assess the purity of DNA and RNA. A ratio of ~1.8 is generally accepted as “pure” for DNA; a ratio of ~2.0 is generally accepted as “pure” for RNA. If the ratio is appreciably lower in either case, it may indicate the presence of protein, pheno ...
DNA Synthesis Activity for Biology 40S
DNA Synthesis Activity for Biology 40S

... Before you hand out the worksheets it is pertinent that you review with students basic DNA information, such as, that DNA is the nucleic acid responsible for storing and transferring genetic information between generations, as well as that it is a large molecule (polymer), made of many repeating uni ...
AVERY and DNA
AVERY and DNA

... In 1928, English scientist FREDERICK GRIFFITH set out to try and find a reason why certain bacteria cause pneumonia. Although he did start with this goal, what happened next would change how the world looks at genetics. Griffith knew that there was two strands of bacteria that could cause pneumonia; ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI

... Give four alternative terms for gene manipulation technology 17) Mention the various kinds of ligases and its origin? 18) Comment on binary vector. 19) Define South- Western hybridization. 20) What is a PCR primer? ...
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DNA nanotechnology



DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.
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