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Homework 3.1 CHEM151: Biochemistry I Prof. Tsai Page 1 of 4 1
Homework 3.1 CHEM151: Biochemistry I Prof. Tsai Page 1 of 4 1

... 14. Which of the following statements about DNA replication in prokaryotes is false? a) DNA synthesis proceeds along both strands at the same time b) DNA synthesis proceeds in both directions from an origin simultaneously. c) Leading strand synthesis occurs in short bursts, creating a series of frag ...
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... For instance, a stretch of DNA could be AATGACCAT - which would code for a different gene than a stretch that read: GGGCCATAG. All in all, there are billions of bases (nucleotides) in cells, which code for all the things an organism needs to function. ...
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... Epigenetics and DNA Methylation: • Differential gene expression allows different cell types to adopt different fates. • Mistakes in gene regulation are associated with a variety of diseases, especially cancer. ...
Objective - Central Magnet School
Objective - Central Magnet School

... identify single base pair differences in DNA • Explain how single base pair changes called single nucleotide polymorphisms (SNPs) can be identified through genetic testing and often correlate to specific diseases or traits. ...
Chapter 12 - useful links
Chapter 12 - useful links

... The History of DNA o Fred Griffith’s experiments inferred that genetic information could be transformed or transferred from one bacterium to another. circa 1928 o Erwin Chargaff showed that the % of nitrogen bases cytosine/guanine, and adenine/thymine, were approximately equal in number. o Oswald Av ...
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Chapter 12 Section 1

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Ch. 10 DNA, RNA, PROTEIN SYNTHESIS

... – Step 1: Helicases (an enzyme) separate the DNA strands by breaking the Hydrogen bonds which connect the nitrogenous bases. Creating the replication fork (the Y-shaped region created when the DNA strands separate. – Step 2: DNA Polymerases (an enzyme) bring free floating nitrogenous bases to the no ...
DNA Ligase Joke (insert laughter here)
DNA Ligase Joke (insert laughter here)

... following replication-both strands are identical—recall semi-conservative:— each new DNA double-helix has one parental strand and one newly-formed strand No enzyme activity necessary ...
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Review 2 - web.biosci.utexas.edu
Review 2 - web.biosci.utexas.edu

... Why is DNA polymerase I considered “distributive” and polymerase III “processive”? What is the main difference between how the leading strand is synthesized and how the lagging strand is synthesized? What are Okazaki fragments? How and why are they formed? What is the role of beta clamp? Is it a par ...
Protein Synthesis
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... Lack nucleus and many of the organelles found in eukaryotes. DNA is located in the cytoplasm. Have a single circular DNA molecule (usually referred to as the cell's chromosome). ...
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... 11. RNA is similar to DNA in that it consists of nucleotides joined together, however RNA does not contain the nucleotide __________, which is replaced by __________. 12. A nucleotide of DNA is composed of a ____________ sugar group, a __________ group and a ____________ ______. 13. The nitrogenous ...
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... injected DNA of phage provides genetic information that makes infected cells produce new viral DNA and proteins, which assemble into new viruses. • DNA doubles prior to mitosis. ...
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... 1. _____________: enzyme which catalyze the _____________________________ (breaking H-Bonds) of the parental double helix. 2. Single-Strand Binding Proteins: proteins which attach and help keep the separated strands apart. 3. __________________: enzyme which relieves stress on the DNA molecule by al ...
Easy DEtEction of MultiplE GEnEs
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... testing, detection of allergic substances and criminal investigations. Genes are so minuscule and imperceptible that until now they could not be tested or analyzed without special equipment. However, a revolutionary system with a simple kit that paves the way for quick visual observation of multiple ...
Gel Electrophoresis
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... Restriction Enzymes – Enzymes that cut DNA Enzymes that cut DNA sequences at specific regions • Hundreds are known • Each one recognizes a specific sequence of nucleotides ...
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... DNA has a 1:1 ratio of pyrimidine and purine bases; the amount of -Guanine = Cytosine, Adenine = Thymine ...
Dna: Hereditary molecules of life
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DNA: The Genetic Material
DNA: The Genetic Material

... DNA polymerase III (pol III) is the main polymerase responsible for the majority of DNA synthesis DNA polymerase III adds nucleotides to the 3’ end of the daughter strand of DNA ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... A tautomeric shift A. is typically induced by high-energy radiation. B. occurs only in prokaryotes. C. can lead to a transversion mutation. D. can produce a transition mutation. ...
Exam #2 KEY
Exam #2 KEY

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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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