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Identifying the Genetic Material
Identifying the Genetic Material

... two separate batches of E. coli bacteria. Because radioactive elements release particles that can be detected with machines, they can be followed, or traced, in a biological process. Scientists could determine whether it was the DNA, the protein, or both that were being transferred into the bacteria ...
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... amplify fragments from them by PCR with suitably chosen pairs of primers that do not amplify any sequences from DNAA itself. Table 1 shows the results of an experiment with primers CL3a and CL8, which are both complementary to sequences in DNA-A (Harrison et al., 1997). Amplification products were o ...
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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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