Metagenomics: DNA sequencing of environmental samples
... axenic culture. Based on these genomic insights, Renesto et al. then used a standard tissue culture medium, supplemented with amino acids implicated by the sequence analysis, to successfully cultivate T. whipplei in the absence of host cells, shortening their doubling time by an order of magnitude34 ...
... axenic culture. Based on these genomic insights, Renesto et al. then used a standard tissue culture medium, supplemented with amino acids implicated by the sequence analysis, to successfully cultivate T. whipplei in the absence of host cells, shortening their doubling time by an order of magnitude34 ...
A general and rapid mutagenesis method using polymerase chain
... misincorporation in 3900 bp using two mutant oligos and three PCR amplifications to introduce a mutation. The method described here minimises misincorporation using only one mutant oligo, two amplifications and a DNA fragment replacing an additional PCR amplification. The experimental design of our ...
... misincorporation in 3900 bp using two mutant oligos and three PCR amplifications to introduce a mutation. The method described here minimises misincorporation using only one mutant oligo, two amplifications and a DNA fragment replacing an additional PCR amplification. The experimental design of our ...
guidelines
... tubes, plates, pipette tips, boxes for storing, but also pens and pencils!) should be treated separately from those ones used in other labs! These kinds of products should be autoclaved and/or UV sterilized. ...
... tubes, plates, pipette tips, boxes for storing, but also pens and pencils!) should be treated separately from those ones used in other labs! These kinds of products should be autoclaved and/or UV sterilized. ...
Podcast 4 Handout - Chromosome 18 Registry and Research Society
... The large blue box indicates the size of the smallest deletion or duplication that could be found using the conventional cytogenetics that produces the karyotype that you have seen many times. This box includes five million base pairs and would cover 6.5 yards on the football field. This means that ...
... The large blue box indicates the size of the smallest deletion or duplication that could be found using the conventional cytogenetics that produces the karyotype that you have seen many times. This box includes five million base pairs and would cover 6.5 yards on the football field. This means that ...
dna
... How DNA Controls the Workings of the Cell Below are two partial sequences of DNA bases (shown for only one strand of DNA) Sequence 1 is from a human and sequence 2 is from a cow. In both humans and cows, this sequence is part of a set of instructions for controlling a bodily function. In this case, ...
... How DNA Controls the Workings of the Cell Below are two partial sequences of DNA bases (shown for only one strand of DNA) Sequence 1 is from a human and sequence 2 is from a cow. In both humans and cows, this sequence is part of a set of instructions for controlling a bodily function. In this case, ...
6th Year Biology Higher Level Wesley Hammond DNA and RNA
... Isolated DNA is cut into fragments using enzymes (restriction enzymes) depending on the sequence of bases. ...
... Isolated DNA is cut into fragments using enzymes (restriction enzymes) depending on the sequence of bases. ...
"Polymerase Chain Reaction (PCR)". In: Encyclopedia of Life Sciences
... as the template for amplification. The procedure is very similar to conventional PCR but includes an initial step in which a DNA copy of the RNA template is produced using the enzyme reverse transcriptase. This enzyme, which is of viral origin, is a polymerase and has the unique ability to synthesiz ...
... as the template for amplification. The procedure is very similar to conventional PCR but includes an initial step in which a DNA copy of the RNA template is produced using the enzyme reverse transcriptase. This enzyme, which is of viral origin, is a polymerase and has the unique ability to synthesiz ...
PGD
... Genetic testing for specific disease loci (PCR) Polymerase chain reaction (PCR) -The gene causing the disorder should be confirmed and tested in the ...
... Genetic testing for specific disease loci (PCR) Polymerase chain reaction (PCR) -The gene causing the disorder should be confirmed and tested in the ...
DNA
... •DNA ---Deoxyribose Sugar, RNA--Ribose •What is a similarity of DNA and RNA? •G binds with C in both DNA and RNA •Both have sugar and phosphate backbone ...
... •DNA ---Deoxyribose Sugar, RNA--Ribose •What is a similarity of DNA and RNA? •G binds with C in both DNA and RNA •Both have sugar and phosphate backbone ...
File - Central Dogma of Molecular Biology
... model in which the new DNA strand formed contained 1/2 of the original DNA and 1/2 newly synthesized DNA--one strand was original and one strand was new. • They couldn’t rule out a model where somehow the old DNA stayed together and the newly synthesized DNA strand was ...
... model in which the new DNA strand formed contained 1/2 of the original DNA and 1/2 newly synthesized DNA--one strand was original and one strand was new. • They couldn’t rule out a model where somehow the old DNA stayed together and the newly synthesized DNA strand was ...
Slides on chromosomal changes
... See fig 17-19 It is possible that crossing over between repetitive elements (e.g. transposable elements) generates the various rearrangements Crossing over between elements on a single chromosome can result in a deletion or inversion. If two nonhomologous chromosome each have the same repeated Eleme ...
... See fig 17-19 It is possible that crossing over between repetitive elements (e.g. transposable elements) generates the various rearrangements Crossing over between elements on a single chromosome can result in a deletion or inversion. If two nonhomologous chromosome each have the same repeated Eleme ...
Acquired Copy Number Alterations in Adult Acute Myeloid Leukemia
... recurrence. Log2 ratio dot plots of paired tumor and normal DNA research samples from the same individual were generated from data obtained from the Affymetrix Genome-Wide SNP 6.0 arrays (top plot of each panel) and custom NimbleGen CGH 12 ≤135K array data (bottom plot of each panel). Solid horizont ...
... recurrence. Log2 ratio dot plots of paired tumor and normal DNA research samples from the same individual were generated from data obtained from the Affymetrix Genome-Wide SNP 6.0 arrays (top plot of each panel) and custom NimbleGen CGH 12 ≤135K array data (bottom plot of each panel). Solid horizont ...
Chromosome Structure
... Chromosome Structure into structures called chromosomes, which consist of long chains of DNA and associated proteins. In eukaryotes, DNA molecules are tightly wound around proteins - called histone proteins - which provide structural support and play a role in controlling the activities of the genes ...
... Chromosome Structure into structures called chromosomes, which consist of long chains of DNA and associated proteins. In eukaryotes, DNA molecules are tightly wound around proteins - called histone proteins - which provide structural support and play a role in controlling the activities of the genes ...
D2 - Interchim
... This total nucleic acid and DNA purification kit relies on a new nucleic acid binding mechanism. According to this, negatively charged DNA and RNA molecules interact with negatively charged surfaces by a complexing reaction involving multivalent cations such as magnesium. DNA is released as the resu ...
... This total nucleic acid and DNA purification kit relies on a new nucleic acid binding mechanism. According to this, negatively charged DNA and RNA molecules interact with negatively charged surfaces by a complexing reaction involving multivalent cations such as magnesium. DNA is released as the resu ...
Genomic DNA extraction from small amounts of serum to be used for
... both in health and in disease, the source of this DNA remains enigmatic. It is presumed that circulating DNA in healthy subjects is derived from lymphocytes or other nucleated cells [17]. Recently, several methods have been described for preparing genomic DNA from serum with some of them requiring v ...
... both in health and in disease, the source of this DNA remains enigmatic. It is presumed that circulating DNA in healthy subjects is derived from lymphocytes or other nucleated cells [17]. Recently, several methods have been described for preparing genomic DNA from serum with some of them requiring v ...
COAS_B1_Ch08 Nucleic acids
... DNA molecules in a cell nucleus are replicated before cell division takes place. First, the two • The strands of the molecule are untwisted and unzipped. Free DNA nucleotides pair up with the exposed bases on both strands. They are then linked together by the formation of bonds between their deoxyri ...
... DNA molecules in a cell nucleus are replicated before cell division takes place. First, the two • The strands of the molecule are untwisted and unzipped. Free DNA nucleotides pair up with the exposed bases on both strands. They are then linked together by the formation of bonds between their deoxyri ...
Gene Section MRE11A (MRE11 meiotic recombination 11 homolog A (S. cerevisiae))
... domain of each Rad50 unit. As the Zinc-hook of Rad50 is located at the end of a long coiled-coil domain, this provides a flexible structure in which each DNA end is accessible to additional repair enzymes while being held in close proximity to each other in preparation for re-ligation. Cells lacking ...
... domain of each Rad50 unit. As the Zinc-hook of Rad50 is located at the end of a long coiled-coil domain, this provides a flexible structure in which each DNA end is accessible to additional repair enzymes while being held in close proximity to each other in preparation for re-ligation. Cells lacking ...
Recombinant DNA Technology
... SSRs are found in and near many genes throughout the genome--they are quite common and easy to find. During normal replication of the DNA in the nucleus, DNA polymerase sometimes slips and creates extra copies or deletes a few copies of the repeat. This happens rarely enough that most people inherit ...
... SSRs are found in and near many genes throughout the genome--they are quite common and easy to find. During normal replication of the DNA in the nucleus, DNA polymerase sometimes slips and creates extra copies or deletes a few copies of the repeat. This happens rarely enough that most people inherit ...
DNA the Crown Jewels 2012
... We do not need to sequence the entire 3 billion base pairs of a person’s DNA to distinguish it from another person’s DNA Noncoding regions of DNA (junk DNA) contain sequences that are 3-100 bp in length that are repeated at different locations (loci) along the chromosome. For example- CGGCT may ...
... We do not need to sequence the entire 3 billion base pairs of a person’s DNA to distinguish it from another person’s DNA Noncoding regions of DNA (junk DNA) contain sequences that are 3-100 bp in length that are repeated at different locations (loci) along the chromosome. For example- CGGCT may ...
Chromosome Theory of Inheritance -States that genes or alleles
... -Is the condition of having more than two complete sets of chromosomes, forming a 3n or 4n individual. Rare in animals this condition is fairy frequent in plants -This often results in sterility, but increased vigor of those polyploidy plant individuals ...
... -Is the condition of having more than two complete sets of chromosomes, forming a 3n or 4n individual. Rare in animals this condition is fairy frequent in plants -This often results in sterility, but increased vigor of those polyploidy plant individuals ...
Comparative genomic hybridization
Comparative genomic hybridization is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.This is achieved through the use of competitive fluorescence in situ hybridization. In short, this involves the isolation of DNA from the two sources to be compared, most commonly a test and reference source, independent labelling of each DNA sample with a different fluorophores (fluorescent molecules) of different colours (usually red and green), denaturation of the DNA so that it is single stranded, and the hybridization of the two resultant samples in a 1:1 ratio to a normal metaphase spread of chromosomes, to which the labelled DNA samples will bind at their locus of origin. Using a fluorescence microscope and computer software, the differentially coloured fluorescent signals are then compared along the length of each chromosome for identification of chromosomal differences between the two sources. A higher intensity of the test sample colour in a specific region of a chromosome indicates the gain of material of that region in the corresponding source sample, while a higher intensity of the reference sample colour indicates the loss of material in the test sample in that specific region. A neutral colour (yellow when the fluorophore labels are red and green) indicates no difference between the two samples in that location.CGH is only able to detect unbalanced chromosomal abnormalities. This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. CGH does, however, allow for the exploration of all 46 human chromosomes in single test and the discovery of deletions and duplications, even on the microscopic scale which may lead to the identification of candidate genes to be further explored by other cytological techniques.Through the use of DNA microarrays in conjunction with CGH techniques, the more specific form of array CGH (aCGH) has been developed, allowing for a locus-by-locus measure of CNV with increased resolution as low as 100 kilobases. This improved technique allows for the aetiology of known and unknown conditions to be discovered.