Metabolic downregulation during diapause in embryos of Artemia
... after the environmental conditions become unfavorable and generally resumes after the conditions return to normal (Drinkwater and Crowe 1987, Hahn and Denlinger 2007). Diapausing animals resume normal development only after diapause is terminated due to exposure to termination cues such as dehydrati ...
... after the environmental conditions become unfavorable and generally resumes after the conditions return to normal (Drinkwater and Crowe 1987, Hahn and Denlinger 2007). Diapausing animals resume normal development only after diapause is terminated due to exposure to termination cues such as dehydrati ...
Physiological Strategies During Animal Diapause: Lessons from
... the lake. These embryos enter diapause at the gastrula stage (Clegg and Conte, 1980). Although the developmental cessation is complete by the time embryos are released by the female into the water column, the metabolic depression requires several days post release. For habitats such as the Great Sal ...
... the lake. These embryos enter diapause at the gastrula stage (Clegg and Conte, 1980). Although the developmental cessation is complete by the time embryos are released by the female into the water column, the metabolic depression requires several days post release. For habitats such as the Great Sal ...
Glycerol transport and phosphoenolpyruvate
... the neighbouring Phe-232 had been replaced with a serine were able to grow on glycerol as the sole carbon source, even when the general PTS proteins enzyme I and HPr were not functional (Wehtje et al., 1995). Glycerol kinase in these mutants was therefore assumed to possess elevated activity, render ...
... the neighbouring Phe-232 had been replaced with a serine were able to grow on glycerol as the sole carbon source, even when the general PTS proteins enzyme I and HPr were not functional (Wehtje et al., 1995). Glycerol kinase in these mutants was therefore assumed to possess elevated activity, render ...
Amino Acids in Maturation Medium and Presence
... porcine oocytes to achieve male pronuclear (MPN) formation when they are matured and penetrated in vitro under various culture conditions. When cumulus-enclosed oocytes were cultured for 24-48 h in modified Whitten's medium (pH 7.4) supplemented with 10% porcine follicular fluid, 10 IU eCG/ml, and 1 ...
... porcine oocytes to achieve male pronuclear (MPN) formation when they are matured and penetrated in vitro under various culture conditions. When cumulus-enclosed oocytes were cultured for 24-48 h in modified Whitten's medium (pH 7.4) supplemented with 10% porcine follicular fluid, 10 IU eCG/ml, and 1 ...
Saffron (Crocus sativus L.) Tissue Culture
... micropropagation of saffron using direct or indirect shoot induction or plantlet regeneration through somatic embryogenesis followed by microcorm production offers the capability to produce large quantities of propagating material in short duration of time, however, the protocols available so far ne ...
... micropropagation of saffron using direct or indirect shoot induction or plantlet regeneration through somatic embryogenesis followed by microcorm production offers the capability to produce large quantities of propagating material in short duration of time, however, the protocols available so far ne ...
Triglyceride Measurements: a Review of Methods and Interferences
... Accuracy and precision guidelines are extremely difficult to determine, owing to broad biological variability both within and among individuals. Here, we review the major triglyceride ...
... Accuracy and precision guidelines are extremely difficult to determine, owing to broad biological variability both within and among individuals. Here, we review the major triglyceride ...
The Future of Human Embryo Culture Media – Or Have We
... The ability to culture tissue samples was pioneered in the early 1900s, and during this time the importance of using a biologically defined media was acknowledged. A biologically defined medium was beneficial in that it could be reproduced at different times and in different laboratories. It can als ...
... The ability to culture tissue samples was pioneered in the early 1900s, and during this time the importance of using a biologically defined media was acknowledged. A biologically defined medium was beneficial in that it could be reproduced at different times and in different laboratories. It can als ...
Energy Metabolism Regulating Mammalian Oocyte
... process. In most mammalian species, the oocyte resumes meiosis in the preovulatory follicle shortly before ovulation. However, meiotic resumption occurs after the dog oocyte is released from the follicle (Songsasen & Wildt, 2007). At the initiation of meiotic resumption, the nucleus of the oocyte mi ...
... process. In most mammalian species, the oocyte resumes meiosis in the preovulatory follicle shortly before ovulation. However, meiotic resumption occurs after the dog oocyte is released from the follicle (Songsasen & Wildt, 2007). At the initiation of meiotic resumption, the nucleus of the oocyte mi ...
REGULATION OF PYRUVATE KINASE AND GLYCEROL
... physiological process that delays development until there is a specific stimulus that triggers the return to normal growth (Dickson, 1949). This is especially important for many insect species with an annual life-cycle because growth and development is halted in the cold months of the year. For exam ...
... physiological process that delays development until there is a specific stimulus that triggers the return to normal growth (Dickson, 1949). This is especially important for many insect species with an annual life-cycle because growth and development is halted in the cold months of the year. For exam ...
The impact of sperm metabolism during in vitro storage: the stallion
... numerous. Sperm motility is not only lost as a consequence of lipid peroxidation due to ROS attack [20], but also due to the depletion of ATP [21]. With respect to the storage of stallion spermatozoa for AI, a better understanding of metabolic processes will allow tailoring of the media constituents ...
... numerous. Sperm motility is not only lost as a consequence of lipid peroxidation due to ROS attack [20], but also due to the depletion of ATP [21]. With respect to the storage of stallion spermatozoa for AI, a better understanding of metabolic processes will allow tailoring of the media constituents ...
PDF
... composed of non-neutral lipid or synthesized during the earlier cleavage stages prior to incubation in radioactive medium (Fig. Ad). Silver grains were also found ...
... composed of non-neutral lipid or synthesized during the earlier cleavage stages prior to incubation in radioactive medium (Fig. Ad). Silver grains were also found ...
Oxidative stress and protection against reactive oxygen
... of development, and the culture conditions. Several exogenous factors and culture conditions can enhance the production of ROS by embryos. ROS can alter most types of cellular molecules, and also induce development block and retardation. Multiple mechanisms of embryo protection against ROS exist, an ...
... of development, and the culture conditions. Several exogenous factors and culture conditions can enhance the production of ROS by embryos. ROS can alter most types of cellular molecules, and also induce development block and retardation. Multiple mechanisms of embryo protection against ROS exist, an ...
Carbon and electron flow in Clostridium butyricum
... suggesting this enzyme may be the bottleneck for carbon flux to butyrate biosynthesis providing an explanation for the accumulation of large amounts of intracellular acetyl-CoA, and (iv) high levels of NADH were found in the cell. Oxidation of NADH by 1,3-PD dehydrogenase was linked to the productio ...
... suggesting this enzyme may be the bottleneck for carbon flux to butyrate biosynthesis providing an explanation for the accumulation of large amounts of intracellular acetyl-CoA, and (iv) high levels of NADH were found in the cell. Oxidation of NADH by 1,3-PD dehydrogenase was linked to the productio ...
Metabolism in the pre-implantation oocyte and embryo
... COCs to “collection” media containing different concentrations of glucose can have a dramatic effect on post-fertilisation embryo development (Frank et al., 2013). Aspiring to determine the precise differences between the metabolism of in vivo and in vitro matured COCs is not possible, as in vivo de ...
... COCs to “collection” media containing different concentrations of glucose can have a dramatic effect on post-fertilisation embryo development (Frank et al., 2013). Aspiring to determine the precise differences between the metabolism of in vivo and in vitro matured COCs is not possible, as in vivo de ...
View PDF - CiteSeerX
... Fermentation of triacetin and glycerol by Acetobacterium sp. No energy is conserved by acetate excretion* R. Emde and B. Schink** Fachbereich Biologie-Mikrobiologie, Philipps-Universitfit, D-3550 Marburg, Federal Republic of Germany Abstract. Two strains of homoacetogenic bacteria similar to Acetoba ...
... Fermentation of triacetin and glycerol by Acetobacterium sp. No energy is conserved by acetate excretion* R. Emde and B. Schink** Fachbereich Biologie-Mikrobiologie, Philipps-Universitfit, D-3550 Marburg, Federal Republic of Germany Abstract. Two strains of homoacetogenic bacteria similar to Acetoba ...
Fixation
... Each fixative has specific properties and disadvantages. Their varied effects to tissues would require careful selection of fixative when specific cellular substances is to be studied. ...
... Each fixative has specific properties and disadvantages. Their varied effects to tissues would require careful selection of fixative when specific cellular substances is to be studied. ...
Fixation
... Each fixative has specific properties and disadvantages. Their varied effects to tissues would require careful selection of fixative when specific cellular substances is to be studied. ...
... Each fixative has specific properties and disadvantages. Their varied effects to tissues would require careful selection of fixative when specific cellular substances is to be studied. ...
Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid
... Background: Due to high neutral lipids accumulation in the cytoplasm, in vitro-produced embryos from Bos primigenius indicus and their crosses are more sensitive to chilling and cryopreservation than those from Bos primigenius taurus. The objective of the present study was to evaluate the effects of ...
... Background: Due to high neutral lipids accumulation in the cytoplasm, in vitro-produced embryos from Bos primigenius indicus and their crosses are more sensitive to chilling and cryopreservation than those from Bos primigenius taurus. The objective of the present study was to evaluate the effects of ...
Nutritional requirements The composition of medium for the tissue
... Sulphur is provided in the form of sulphates. Besides, the sulphur containing amino acids like L-cysteine, L-methionine and glutathione are satisfactory sources for sulphur. Calcium and Magnesium The optimal concentration of Ca required is 3mM. An antagonism between Ca and Mg has been demonstrated a ...
... Sulphur is provided in the form of sulphates. Besides, the sulphur containing amino acids like L-cysteine, L-methionine and glutathione are satisfactory sources for sulphur. Calcium and Magnesium The optimal concentration of Ca required is 3mM. An antagonism between Ca and Mg has been demonstrated a ...
journal club CO2 Cell Paper
... to generate rapid autonomic and behavioral responses to threatening stimuli, such as a predator in the forest, a bully at the office, or an aversive stimulus in the laboratory….. The Amygdala, a collection of nuclei buried in the temporal lobe of the brain, is essential for both innate and learned f ...
... to generate rapid autonomic and behavioral responses to threatening stimuli, such as a predator in the forest, a bully at the office, or an aversive stimulus in the laboratory….. The Amygdala, a collection of nuclei buried in the temporal lobe of the brain, is essential for both innate and learned f ...
Freezing in a glass
... and lids should be tight fitting. WECK Round Rim jars with plastic “Keep Fresh “ covers are perfect for freezing foods. The ¼ liter mold jars and the 1 liter tulip jars have straight sides and 3.875 inch opening, and the “Keep Fresh” lids fit securely on the jars. When glass jars are used for freezi ...
... and lids should be tight fitting. WECK Round Rim jars with plastic “Keep Fresh “ covers are perfect for freezing foods. The ¼ liter mold jars and the 1 liter tulip jars have straight sides and 3.875 inch opening, and the “Keep Fresh” lids fit securely on the jars. When glass jars are used for freezi ...
(Vibrcgen-Zellmiihle, Fa. E. Buhl er, Tiibingen, Germany). The results
... Eater, W . K . G l y c e r o l ...
... Eater, W . K . G l y c e r o l ...
Influence of oxygen deficiency and the role of specific amino acids in
... freezing. The plant vitrification solution PVS3 [5], is the most effective for garlic allowing higher regrowth rates than other vitrification solutions [6]. The genebank at IPK comprises one of the largest cryo-collections worldwide with more than 1500 accessions, the number of garlic accessions amo ...
... freezing. The plant vitrification solution PVS3 [5], is the most effective for garlic allowing higher regrowth rates than other vitrification solutions [6]. The genebank at IPK comprises one of the largest cryo-collections worldwide with more than 1500 accessions, the number of garlic accessions amo ...
Datasheet - BosterBio
... to a year without functional compromise. Lyophilized antibodies must be reconstituted before using. Prior to use, after briefly centrifuge the vial at 12,000 x g for 5-10 seconds. This will insure complete recovery of vial contents. For long term storage, we recommend storing small, single-use aliqu ...
... to a year without functional compromise. Lyophilized antibodies must be reconstituted before using. Prior to use, after briefly centrifuge the vial at 12,000 x g for 5-10 seconds. This will insure complete recovery of vial contents. For long term storage, we recommend storing small, single-use aliqu ...
Cryopreservation
Cryopreservation or cryoconservation is a process where cells, whole tissues, or any other substances susceptible to damage caused by chemical reactivity or time are preserved by cooling to sub-zero temperatures. At low enough temperatures, any enzymatic or chemical activity which might cause damage to the material in question is effectively stopped. Cryopreservation methods seek to reach low temperatures without causing additional damage caused by the formation of ice during freezing. Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termed cryoprotectants. New methods are constantly being investigated due to the inherent toxicity of many cryoprotectants.By default it should be considered that cryopreservation alters or compromises the structure and function of cells unless it is proven otherwise for a particular cell population.