Glycogen Slides from Class
... The cAMP binds to protein kinase A (PKA), a cAMP-dependent protein kinase, activating it. PKA in turn phosphorylates other downstream target proteins including phosphorylase kinase (PhosK) and glycogen synthase (GS). The phosphorylation of PhosK leads to its activation. Conversely, phosphorylatio ...
... The cAMP binds to protein kinase A (PKA), a cAMP-dependent protein kinase, activating it. PKA in turn phosphorylates other downstream target proteins including phosphorylase kinase (PhosK) and glycogen synthase (GS). The phosphorylation of PhosK leads to its activation. Conversely, phosphorylatio ...
21. Membranes
... ii. By the same token, the cytoskeleton can hold membrane components in place. b. Temperature, lateral motion, and hydrocarbons i. As temperature lowers, phospholipids will move more slowly ii. At a certain point, this means that a membrane will become a solid, rather than the liquid it was more lik ...
... ii. By the same token, the cytoskeleton can hold membrane components in place. b. Temperature, lateral motion, and hydrocarbons i. As temperature lowers, phospholipids will move more slowly ii. At a certain point, this means that a membrane will become a solid, rather than the liquid it was more lik ...
RLIMS-P Annotation guidelines This document describes some
... “PAG, the phosphoprotein associated with glycosphingolipid-enriched microdomains (GEM), negatively regulates Src family kinases by recruiting C-terminal Src kinase (Csk) to the membrane, where Csk phosphorylates the inhibitory tyrosine of the Src kinases.” PMID: 18085663. ...
... “PAG, the phosphoprotein associated with glycosphingolipid-enriched microdomains (GEM), negatively regulates Src family kinases by recruiting C-terminal Src kinase (Csk) to the membrane, where Csk phosphorylates the inhibitory tyrosine of the Src kinases.” PMID: 18085663. ...
proteins
... of biological molecules to the functioning of living cells and to human health • Molecular interactions, such as those between the gene for lactase production, the enzyme lactase, and the milk sugar lactose, drive all biological processes ...
... of biological molecules to the functioning of living cells and to human health • Molecular interactions, such as those between the gene for lactase production, the enzyme lactase, and the milk sugar lactose, drive all biological processes ...
Human Proteome advertising miniposter (PDF)
... A proximity ligation assay uses a pair of oligonucleotide labeled antibodies binding to different epitopes on a protein, or to epitopes in close proximity on two proteins in a complex. Used for detection, visualization and quantification of single proteins or protein-protein interactions. ...
... A proximity ligation assay uses a pair of oligonucleotide labeled antibodies binding to different epitopes on a protein, or to epitopes in close proximity on two proteins in a complex. Used for detection, visualization and quantification of single proteins or protein-protein interactions. ...
FR in detergent-insoluble complexes - Journal of Cell Science
... on cells of the nervous system (Buj-Bello et al., 1997; Klein et al., 1997; Peles et al., 1997) and a ligand effect, when in released form, on transmembrane receptors (Park and Sanchez, 1997). A key determinant of functional activity of GPI-proteins (Hanada et al., 1995; Rothberg et al., 1990) appea ...
... on cells of the nervous system (Buj-Bello et al., 1997; Klein et al., 1997; Peles et al., 1997) and a ligand effect, when in released form, on transmembrane receptors (Park and Sanchez, 1997). A key determinant of functional activity of GPI-proteins (Hanada et al., 1995; Rothberg et al., 1990) appea ...
DOPAMINE RECEPTORS
... Although biochemical studies gave some indications of further heterogeneity of these dopamine receptor subtypes, it was not until the late 1980’s that the true extent of this was revealed by the application of gene cloning techniques to the dopamine receptors. This showed that there were at least fi ...
... Although biochemical studies gave some indications of further heterogeneity of these dopamine receptor subtypes, it was not until the late 1980’s that the true extent of this was revealed by the application of gene cloning techniques to the dopamine receptors. This showed that there were at least fi ...
Anti-HSP90 Catalog# SMC-149 A/B Size: 50/200µg This product is
... HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms a and B, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90a exists predominantl ...
... HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms a and B, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90a exists predominantl ...
Document
... the most abundant protein in the body, accounting for 25-35% of the body’s total protein content. • Procollagen, produced in the rough ER, is the initial precursor to collagen and consists of 3 polypeptide strands stabilized by disulfide bonds and eventually wound into a triple helix. Each polypeptid ...
... the most abundant protein in the body, accounting for 25-35% of the body’s total protein content. • Procollagen, produced in the rough ER, is the initial precursor to collagen and consists of 3 polypeptide strands stabilized by disulfide bonds and eventually wound into a triple helix. Each polypeptid ...
Minimizing filtration losses
... are a couple of reasons for this. One, during process development, small process volumes make it relatively simple to proceed quickly from process step to process step. Solubility problems may not have time to become readily apparent. Two, it’s a lot easier to see an aggregation or precipitation pro ...
... are a couple of reasons for this. One, during process development, small process volumes make it relatively simple to proceed quickly from process step to process step. Solubility problems may not have time to become readily apparent. Two, it’s a lot easier to see an aggregation or precipitation pro ...
Cannabinoid Signaling and Lipid Rafts
... synthesis is influenced by lipid rafts. The plasma membrane is a highly disordered phospholipid by-layer that consists of compartmentalized microdomains. These microdomains that are enriched in cholesterol, sphingolipids, and arachidonic acid are referred to as lipid rafts. Rafts can be conceived as ...
... synthesis is influenced by lipid rafts. The plasma membrane is a highly disordered phospholipid by-layer that consists of compartmentalized microdomains. These microdomains that are enriched in cholesterol, sphingolipids, and arachidonic acid are referred to as lipid rafts. Rafts can be conceived as ...
Bio 263/F94/T2
... a. H bonds between polar amino acid R groups b. covalent bonds c. H bonds between amide (peptide) linkages oriented parallel to the molecule's axis d. acidobasic combinations e. H bonds between amide linkages, oriented perpendicular to the molecule's axis 21. The final protein conformation (the 3-D ...
... a. H bonds between polar amino acid R groups b. covalent bonds c. H bonds between amide (peptide) linkages oriented parallel to the molecule's axis d. acidobasic combinations e. H bonds between amide linkages, oriented perpendicular to the molecule's axis 21. The final protein conformation (the 3-D ...
chapt05_lecture
... from packing tightly • Most membranes also contain sterols such as cholesterol, which can either increase or decrease membrane fluidity, depending on the temperature ...
... from packing tightly • Most membranes also contain sterols such as cholesterol, which can either increase or decrease membrane fluidity, depending on the temperature ...
1 Food intake regulation
... PYY, peptide tyrosine-tyrosine (P10082) , released by by cells in the ileum and colon in response to feeding and acts as an anorexigen Insulin, (P01308) peptide hormone released by b-cells in the islet of Langerhans of the (endocrine) pancreas (hence its name insulin) in response to elevated levels ...
... PYY, peptide tyrosine-tyrosine (P10082) , released by by cells in the ileum and colon in response to feeding and acts as an anorexigen Insulin, (P01308) peptide hormone released by b-cells in the islet of Langerhans of the (endocrine) pancreas (hence its name insulin) in response to elevated levels ...
File
... An alternate approach to the template-mediated association of peptide chains is the use of disulfide bridges to tether peptide modules and induce local folding. This strategy was utilized in the classical design of some members of the betabellin family: betadoublet176 and betabellins 14D128 and 15 ...
... An alternate approach to the template-mediated association of peptide chains is the use of disulfide bridges to tether peptide modules and induce local folding. This strategy was utilized in the classical design of some members of the betabellin family: betadoublet176 and betabellins 14D128 and 15 ...
Explanation of Scaffold`s Display Options - Proteome Software
... % of total spectra: This is the percentage of the total number of spectra which is assigned to the protein in question. This number is the number of assigned spectra for this protein divided by the total spectra in the sample (as seen in the Load Data View). Assigned spectra: This is the number of s ...
... % of total spectra: This is the percentage of the total number of spectra which is assigned to the protein in question. This number is the number of assigned spectra for this protein divided by the total spectra in the sample (as seen in the Load Data View). Assigned spectra: This is the number of s ...
Kojo Mensa-Wilmot* and Paul T.Englund Department of Biological
... the GPI-PLC coding sequence; there are also translational termination codons (TAA; outlined) to block synthesis of any protein encoded upstream of the GPI-PLC gene. The downstream primer (Figure 1A) contains 21 nucleotides complementary to the 3' end of GPI-PLC coding sequence (bold), a //mdllll sit ...
... the GPI-PLC coding sequence; there are also translational termination codons (TAA; outlined) to block synthesis of any protein encoded upstream of the GPI-PLC gene. The downstream primer (Figure 1A) contains 21 nucleotides complementary to the 3' end of GPI-PLC coding sequence (bold), a //mdllll sit ...
Protein basics - Crop Genebank Knowledge Base
... The three-dimensional structure that proteins have is a direct result of interactions with its internal environment. As a consequence, knowing how proteins are structured tells us a lot about how they perform their tasks in the cell. For instance, in aqueous environments, the hydrophobic R-groups ar ...
... The three-dimensional structure that proteins have is a direct result of interactions with its internal environment. As a consequence, knowing how proteins are structured tells us a lot about how they perform their tasks in the cell. For instance, in aqueous environments, the hydrophobic R-groups ar ...
protein translocation.
... ribosomes are "membrane-bound". • The proteins pass into the ER along the Golgi and then through the plasma membrane, unless they have signals that cause retention at one of the steps on the pathway. • They may also be directed to other organelles, such as endosomes or lysosomes. ...
... ribosomes are "membrane-bound". • The proteins pass into the ER along the Golgi and then through the plasma membrane, unless they have signals that cause retention at one of the steps on the pathway. • They may also be directed to other organelles, such as endosomes or lysosomes. ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).