Stacks off tracks
... expressing fluorescently tagged AtCASP, a truncated AtCASP-ΔCC lacking the coiled-coil ...
... expressing fluorescently tagged AtCASP, a truncated AtCASP-ΔCC lacking the coiled-coil ...
Aalborg Universitet
... Heart failure (HF) is associated with activation of the renin-angiotensin system (RAS) and sustained increased vasopressin (AVP) release from the pituitary gland [1–5]. RAS and AVP have been shown to play a role in the kidneys by taking part in the development of hyponatremia and water retention. Hy ...
... Heart failure (HF) is associated with activation of the renin-angiotensin system (RAS) and sustained increased vasopressin (AVP) release from the pituitary gland [1–5]. RAS and AVP have been shown to play a role in the kidneys by taking part in the development of hyponatremia and water retention. Hy ...
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... that grn acts upstream of unc-5 and regulates its expression. To test this hypothesis, we analysed unc-5 mRNA levels in grn/+ and grn/grn mutant embryos. We determined unc-5 expression by in situ hybridization with fluorescent RNA probes and labelled dMN (aCC and RP2) cell bodies with protein marker ...
... that grn acts upstream of unc-5 and regulates its expression. To test this hypothesis, we analysed unc-5 mRNA levels in grn/+ and grn/grn mutant embryos. We determined unc-5 expression by in situ hybridization with fluorescent RNA probes and labelled dMN (aCC and RP2) cell bodies with protein marker ...
Decision making by p53: life, death and cancer
... and exerts very little, if any, effect on cell fate. However, in response to various types of stress, p53 becomes activated; this is reflected in elevated protein levels, as well as augmented biochemical capabilities. As a consequence of p53 activation, cells can undergo marked phenotypic changes, r ...
... and exerts very little, if any, effect on cell fate. However, in response to various types of stress, p53 becomes activated; this is reflected in elevated protein levels, as well as augmented biochemical capabilities. As a consequence of p53 activation, cells can undergo marked phenotypic changes, r ...
... and out of Golgi bodies using photobleaching recovery techniques. In these experiments, we selectively photobleached a single Golgi body and then monitored recovery into the bleached structure over time. A significant recovery indicates that the bleached molecules are exchanging with fluorescent poo ...
Ectopic Expression of AINTEGUMENTA in Arabidopsis Plants
... this construct was transformed into wild-type Arabidopsis plants. As shown in Figure 1E–H, 35S::ANT confers persistent ANT expression in developing floral organs. Approximately one-half of the transgenic plants (66 out of 127 plants) produced larger than normal flowers (Fig. 2A). Of the other transf ...
... this construct was transformed into wild-type Arabidopsis plants. As shown in Figure 1E–H, 35S::ANT confers persistent ANT expression in developing floral organs. Approximately one-half of the transgenic plants (66 out of 127 plants) produced larger than normal flowers (Fig. 2A). Of the other transf ...
The COOH-terminal domain of agrin signals via a synaptic receptor
... accumulation of acetylcholine receptors (AChRs)* at the neuromuscular junction is now widely accepted (for review see Hoch, 1999). Although a complete account of how agrin triggers such changes in AChR distribution has yet to be established, much of what we know about this process has been learned f ...
... accumulation of acetylcholine receptors (AChRs)* at the neuromuscular junction is now widely accepted (for review see Hoch, 1999). Although a complete account of how agrin triggers such changes in AChR distribution has yet to be established, much of what we know about this process has been learned f ...
ภาพนิ่ง 1
... guard cells of Commelina resulted in stomatal closure after the stomata were treated briefly with UV irradiation to activate the hormone --- that is, release it ...
... guard cells of Commelina resulted in stomatal closure after the stomata were treated briefly with UV irradiation to activate the hormone --- that is, release it ...
Murine Regulatory T Cells Contain Hyperproliferative and Death
... cell surface staining for CD4 and CD8 as well as fixable Live/ Dead staining for dead cells, cultured cells were intracellularly stained for Foxp3. Comparison between total CD4+Foxp3+ Treg and CD4+Foxp32 Tcon revealed much weaker proliferation of Treg than Tcon (Fig. 1A), which is consistent with pr ...
... cell surface staining for CD4 and CD8 as well as fixable Live/ Dead staining for dead cells, cultured cells were intracellularly stained for Foxp3. Comparison between total CD4+Foxp3+ Treg and CD4+Foxp32 Tcon revealed much weaker proliferation of Treg than Tcon (Fig. 1A), which is consistent with pr ...
Receptor-Like Activity Evoked by Extracellular ADP in Arabidopsis
... a hyperpolarization-activated (i.e. activated by increasingly negative voltage) Ca2+-permeable conductance (Fig. 1A), very similar to that characterized in response to 20 mM extracellular ATP in this cell type (Demidchik et al., 2009). The concentration used is representative of wounding levels (Son ...
... a hyperpolarization-activated (i.e. activated by increasingly negative voltage) Ca2+-permeable conductance (Fig. 1A), very similar to that characterized in response to 20 mM extracellular ATP in this cell type (Demidchik et al., 2009). The concentration used is representative of wounding levels (Son ...
Specific Organization of Golgi Apparatus in Plant Cells
... myosin 6 binds to the Golgi apparatus membrane through the protein optineurin and participates in the maintenance of the Golgi apparatus morphology and transfer of vesicles to the plasma membrane [48]. With the Golgi apparatus membranes non-muscular myosin 2 and myosin 1b are bound, which are also i ...
... myosin 6 binds to the Golgi apparatus membrane through the protein optineurin and participates in the maintenance of the Golgi apparatus morphology and transfer of vesicles to the plasma membrane [48]. With the Golgi apparatus membranes non-muscular myosin 2 and myosin 1b are bound, which are also i ...
Glycogen Synthase Sensitivity to Insulin and Glucose-6
... Generation of Rat-1 cell lines stably expressing glycogen synthase. In a previous work, we studied the effects of Ser→Ala substitution at individual phosphorylation sites on the activation state of glycogen synthase transiently expressed in COS cells (4,5). Although this cell model was effective for ...
... Generation of Rat-1 cell lines stably expressing glycogen synthase. In a previous work, we studied the effects of Ser→Ala substitution at individual phosphorylation sites on the activation state of glycogen synthase transiently expressed in COS cells (4,5). Although this cell model was effective for ...
Polarization of the endomembrane system is an early event in fucoid
... that establish cell polarity and determine the site of tip growth. A common feature of polarity establishment is targeting endocytosis and exocytosis (secretion) to localized cortical domains. We have investigated the spatiotemporal development of endomembrane asymmetry in photopolarizing zygotes, a ...
... that establish cell polarity and determine the site of tip growth. A common feature of polarity establishment is targeting endocytosis and exocytosis (secretion) to localized cortical domains. We have investigated the spatiotemporal development of endomembrane asymmetry in photopolarizing zygotes, a ...
Control of Leaf and Vein Development by Auxin
... lamina. These observations raise the questions of (1) what are the specific signaling pathways that sculpt leaf shape and vascular patterns, (2) to what degree lamina growth and vascular development share common genetic control, and finally (3) how coordination between leaf and vascular development ...
... lamina. These observations raise the questions of (1) what are the specific signaling pathways that sculpt leaf shape and vascular patterns, (2) to what degree lamina growth and vascular development share common genetic control, and finally (3) how coordination between leaf and vascular development ...
Patterson - Iowa State University
... at position three in which sepals, petals, and filaments are preparing for abscission. Right, Longitudinal section of wild-type inflorescence at position seven showing the elongated cells of the remaining abscission layer proximal to the main body of the plant. B, Longitudinal section showing chitin ...
... at position three in which sepals, petals, and filaments are preparing for abscission. Right, Longitudinal section of wild-type inflorescence at position seven showing the elongated cells of the remaining abscission layer proximal to the main body of the plant. B, Longitudinal section showing chitin ...
Sensitive Interaction between Raptor a
... mTOR in buffers containing the detergent Triton X-100, suggesting that the GL-mTOR interaction does not require raptor. In addition to HEK-293T cells, we also detected the endogenous mTOR-GL complex in mouse NIH-3T3 and C2C12 cell lines (data not shown). Furthermore, when overexpressed in HEK-293T ...
... mTOR in buffers containing the detergent Triton X-100, suggesting that the GL-mTOR interaction does not require raptor. In addition to HEK-293T cells, we also detected the endogenous mTOR-GL complex in mouse NIH-3T3 and C2C12 cell lines (data not shown). Furthermore, when overexpressed in HEK-293T ...
Actin-Dependent and -Independent Functions of
... (Folkers et al., 2002). To provide support for the idea that cortical microtubules (and not other types of microtubule configurations, such as endoplasmic microtubules) are the basis of branching in trichomes, we analyzed the branching process at increased spatiotemporal resolution. Analyses were per ...
... (Folkers et al., 2002). To provide support for the idea that cortical microtubules (and not other types of microtubule configurations, such as endoplasmic microtubules) are the basis of branching in trichomes, we analyzed the branching process at increased spatiotemporal resolution. Analyses were per ...
Fibronectin and Other Adhesive Glycoproteins
... vertebrate development, presumably by mediating a variety of adhesive and migratory events. Targeted inactivation of the fibronectin gene is lethal at embryonic day 8.5 in embryos homozygous for the disruption (George et al. 1993). Plasma fibronectin is also important for thrombosis. Conditional fib ...
... vertebrate development, presumably by mediating a variety of adhesive and migratory events. Targeted inactivation of the fibronectin gene is lethal at embryonic day 8.5 in embryos homozygous for the disruption (George et al. 1993). Plasma fibronectin is also important for thrombosis. Conditional fib ...
List of omics@vib project topics
... State of the art bioinformatics at genome-wide level to identify, validate and model novel genetic factors that determine anti-inflammation therapy with glucocorticoids in several model systems >>> See also 'Bioinformatics'/ 'Proteomics' e-mail Jan Steyaert Lab Next generation sequencing technologie ...
... State of the art bioinformatics at genome-wide level to identify, validate and model novel genetic factors that determine anti-inflammation therapy with glucocorticoids in several model systems >>> See also 'Bioinformatics'/ 'Proteomics' e-mail Jan Steyaert Lab Next generation sequencing technologie ...
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... the cell of defective or superfluous organelles, and autophagy would appear to be the sole cellular process by which this occurs. Mitochondria accumulate oxidative damage with age, and, in cells with defective autophagy, such damaged mitochondria fail to be eliminated and accumulate to high levels ( ...
... the cell of defective or superfluous organelles, and autophagy would appear to be the sole cellular process by which this occurs. Mitochondria accumulate oxidative damage with age, and, in cells with defective autophagy, such damaged mitochondria fail to be eliminated and accumulate to high levels ( ...
Lateral root initiation is a probabilistic event whose frequency is set
... Treating roots with 1-naphthaleneacetic acid (NAA), a synthetic auxin that can enter cells without the aid of auxin input carriers, confirms that there is a gradient of responsiveness. After addition of NAA, LRP form along the entire length of the root, but are most concentrated in the basal meriste ...
... Treating roots with 1-naphthaleneacetic acid (NAA), a synthetic auxin that can enter cells without the aid of auxin input carriers, confirms that there is a gradient of responsiveness. After addition of NAA, LRP form along the entire length of the root, but are most concentrated in the basal meriste ...