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24.t Glycolysis

... (Thesl.rnbol@represents thephosphorylgroup-il-o ...
Chemistry - Delhi Public School, Faridabad
Chemistry - Delhi Public School, Faridabad

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File - Mr Weng`s IB Chemistry

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Chapter 1-3 Exam Review
Chapter 1-3 Exam Review

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Size-exclusion chromatography



Size-exclusion chromatography (SEC) is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. SEC is a widely used polymer characterization method because of its ability to provide good molar mass distribution (Mw) results for polymers.
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