Construction of mutant and chimeric genes using the polymerase
... segment of DNA within a complex DNA sample is effected by repeated cycles of DNA denaturation and enzymatic synthesis primed by two oligonucleotides complementary to regions within opposite strands of the DNA. In this report a simple and efficient method is described in which PCR methodology is used ...
... segment of DNA within a complex DNA sample is effected by repeated cycles of DNA denaturation and enzymatic synthesis primed by two oligonucleotides complementary to regions within opposite strands of the DNA. In this report a simple and efficient method is described in which PCR methodology is used ...
Demonstration of the ExpandTM PCR System`s Greater Fidelity and
... pUCIQ17, which contains a functional lacIq allele (1). PCR-derived mutations in lacI result in a de-repression of the expression of lacZα and subsequent formation of a functional β-galactosidase enzyme, which can be easily detected on X-Gal indicator plates. ...
... pUCIQ17, which contains a functional lacIq allele (1). PCR-derived mutations in lacI result in a de-repression of the expression of lacZα and subsequent formation of a functional β-galactosidase enzyme, which can be easily detected on X-Gal indicator plates. ...
FAFLP: last word in microbial genotyping?
... implication derived from the same conserved genome sequence. Other fragments will be polymorphic. If no polymorphic fragments are found in a comparison of a group of isolates the implication is that, on the basis of those particular FAFLP conditions, the group constitutes a single strain (clone). Th ...
... implication derived from the same conserved genome sequence. Other fragments will be polymorphic. If no polymorphic fragments are found in a comparison of a group of isolates the implication is that, on the basis of those particular FAFLP conditions, the group constitutes a single strain (clone). Th ...
Breakdown Industrial Digester PowderTM contains bacteria and
... that produce digestive enzymes when added to organic material. The bacteria in OdormuteTM Septic Tank Maintenance release lipase enzymes that attack lipids (fats) releasing fatty acids which are broken down into smaller compounds. The same bacteria also produce Protease enzymes (that break down prot ...
... that produce digestive enzymes when added to organic material. The bacteria in OdormuteTM Septic Tank Maintenance release lipase enzymes that attack lipids (fats) releasing fatty acids which are broken down into smaller compounds. The same bacteria also produce Protease enzymes (that break down prot ...
Can pseudocomplementary peptide nucleic acid nucleases
... short insertions or deletions at the cleavage site. Repair by HR using a DNA template results in gene knock-ins that are either a perfect repair or, if a modified template is introduced, sequence replacement1,2. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding mod ...
... short insertions or deletions at the cleavage site. Repair by HR using a DNA template results in gene knock-ins that are either a perfect repair or, if a modified template is introduced, sequence replacement1,2. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding mod ...
A3.3.1ActionMolecules
... They include thousands of different substances which can be classified into five basic types: structural, regulatory, immunological, transport and catalytic. In this activity, you will focus on the action or catalytic proteins; these proteins, called enzymes, act as catalysts to facilitate chemical ...
... They include thousands of different substances which can be classified into five basic types: structural, regulatory, immunological, transport and catalytic. In this activity, you will focus on the action or catalytic proteins; these proteins, called enzymes, act as catalysts to facilitate chemical ...
Biology 115 Lab 10:Gene Technology
... The DNA that we’ll be using comes from a plasmid. Plasmids are small, circular DNA molecules that occur in bacteria, and are replicated independently of the bacterial chromosome. We can get large amounts of plasmid DNA by growing bacteria containing the plasmid in culture, and then purifying the pla ...
... The DNA that we’ll be using comes from a plasmid. Plasmids are small, circular DNA molecules that occur in bacteria, and are replicated independently of the bacterial chromosome. We can get large amounts of plasmid DNA by growing bacteria containing the plasmid in culture, and then purifying the pla ...
Life on Mars
... check that the PCR is working. A ‘negative control’, without DNA, is carried out to check that samples have not been contaminated during PCR preparation. Positive controls can also be used to exclude so-called “false-positive” results. ...
... check that the PCR is working. A ‘negative control’, without DNA, is carried out to check that samples have not been contaminated during PCR preparation. Positive controls can also be used to exclude so-called “false-positive” results. ...
Using GenomiPhi DNA Amplification Kit for the Representative
... Whole genome amplification method can be applied to linear, genomic DNA and is the basis of our recently launched GenomiPhiTM product. This method employs the unique biochemical properties of Phi29 DNA polymerase, a highly processive enzyme with excellent strand displacement activity, along with ran ...
... Whole genome amplification method can be applied to linear, genomic DNA and is the basis of our recently launched GenomiPhiTM product. This method employs the unique biochemical properties of Phi29 DNA polymerase, a highly processive enzyme with excellent strand displacement activity, along with ran ...
Toxic Effects
... P450 induction together with enzymes associated with it Family 1 & 2 Lipophilic contaminants – flat molecules PCBs, PAH & Dioxins DNA adducts Repair mechanism Metallothionenes rich in SH groups Lower cellular concentrations by sequestration Prevention of damage by removal of xenobiotics before they ...
... P450 induction together with enzymes associated with it Family 1 & 2 Lipophilic contaminants – flat molecules PCBs, PAH & Dioxins DNA adducts Repair mechanism Metallothionenes rich in SH groups Lower cellular concentrations by sequestration Prevention of damage by removal of xenobiotics before they ...
Open file
... effect a person’s phenotype and therefore there physical appearance. This is because they aid in the reactions involved with growth and development. Enzymes have their own nomenclature. They can be categorised by the reactions they catalyse. These are: Oxidase (Oxidisation/reduction reactions), Hydr ...
... effect a person’s phenotype and therefore there physical appearance. This is because they aid in the reactions involved with growth and development. Enzymes have their own nomenclature. They can be categorised by the reactions they catalyse. These are: Oxidase (Oxidisation/reduction reactions), Hydr ...
Enzyme Activity with Graphs
... (1) An enzyme and a SUBSTRATE are in the same area. The substrate is the biological molecule that the enzyme will work on. (2) The enzyme grabs onto the substrate with a special area called the ACTIVE SITE. The active site is a specially shaped area of the enzyme that fits around the substrate. The ...
... (1) An enzyme and a SUBSTRATE are in the same area. The substrate is the biological molecule that the enzyme will work on. (2) The enzyme grabs onto the substrate with a special area called the ACTIVE SITE. The active site is a specially shaped area of the enzyme that fits around the substrate. The ...
Chemistry 110 Enzymes
... product of a series of enzyme-catalyzed reactions inhibits an earlier reaction in a sequence. The inhibition may be competitive or noncompetitive. ¾A proenzyme or zymogen is an inactive form of an enzyme that must have part of its polypeptide chain cleaved before it becomes active. An example is try ...
... product of a series of enzyme-catalyzed reactions inhibits an earlier reaction in a sequence. The inhibition may be competitive or noncompetitive. ¾A proenzyme or zymogen is an inactive form of an enzyme that must have part of its polypeptide chain cleaved before it becomes active. An example is try ...
full size
... product of a series of enzyme-catalyzed reactions inhibits an earlier reaction in a sequence. The inhibition may be competitive or noncompetitive. ¾A proenzyme or zymogen is an inactive form of an enzyme that must have part of its polypeptide chain cleaved before it becomes active. An example is try ...
... product of a series of enzyme-catalyzed reactions inhibits an earlier reaction in a sequence. The inhibition may be competitive or noncompetitive. ¾A proenzyme or zymogen is an inactive form of an enzyme that must have part of its polypeptide chain cleaved before it becomes active. An example is try ...
SEQUENCE ANALYSIS OF GENE CODING FOR PHENOL
... the conversion of phenols to their o-diol derivatives by incorporation of a single hydroxyl group into the substrate. The aim of the present work was to identify and sequenced the gene encoding the phenol hydroxylase in a strain of Aspergillus awamori. A three pairs of oligonucleotide primers were c ...
... the conversion of phenols to their o-diol derivatives by incorporation of a single hydroxyl group into the substrate. The aim of the present work was to identify and sequenced the gene encoding the phenol hydroxylase in a strain of Aspergillus awamori. A three pairs of oligonucleotide primers were c ...
Metal ions in biological system and Potential Medicine
... Nucleases (Restriction Enzymes) Enzymes which carry out hydrolysis of internucleotide linkages in nucleic acids at relatively specific points. ...
... Nucleases (Restriction Enzymes) Enzymes which carry out hydrolysis of internucleotide linkages in nucleic acids at relatively specific points. ...