Real-time PCR-based detection of Bordetella pertussis and
... extension at 72 uC for 10 min. PCR products were cloned using the TOPO TA Cloning system (Invitrogen). Plasmid DNA was isolated using the QIAprep miniprep kit (Qiagen) according to the manufacturer’s instructions. The nucleotide base sequences of the plasmid DNA inserts were determined by nucleotide ...
... extension at 72 uC for 10 min. PCR products were cloned using the TOPO TA Cloning system (Invitrogen). Plasmid DNA was isolated using the QIAprep miniprep kit (Qiagen) according to the manufacturer’s instructions. The nucleotide base sequences of the plasmid DNA inserts were determined by nucleotide ...
Novel Blocked-Cleavable Primers for Quantitative Detection of
... prime DNA synthesis. A cleavable RNA linkage is positioned near the 3’ end of the primer. Following hybridization to a complementary sequence, the terminal blocking group is removed by action of a thermostable RNase H2 enzyme. Importantly, the RNase H2 enzyme, which is simply added as a component of ...
... prime DNA synthesis. A cleavable RNA linkage is positioned near the 3’ end of the primer. Following hybridization to a complementary sequence, the terminal blocking group is removed by action of a thermostable RNase H2 enzyme. Importantly, the RNase H2 enzyme, which is simply added as a component of ...
2.08-11.2 Line Probe Assays
... human resource requirements need to be carefully assessed prior to implementation. It should be noted that supervision in most of the published studies was performed by scientists with postgraduate training in molecular technology. Interpretation of results of line probe assays must be done with car ...
... human resource requirements need to be carefully assessed prior to implementation. It should be noted that supervision in most of the published studies was performed by scientists with postgraduate training in molecular technology. Interpretation of results of line probe assays must be done with car ...
Development of an internally controlled real-time PCR
... evaluation of the PCR was limited. In the present study, C. psittaci DNA was detected in eight respiratory samples obtained from eight patients (c. 30% (8 ⁄ 27) of the annual reported cases in The Netherlands) [3]. One of these samples had been shown previously to be PCR-positive with a different pr ...
... evaluation of the PCR was limited. In the present study, C. psittaci DNA was detected in eight respiratory samples obtained from eight patients (c. 30% (8 ⁄ 27) of the annual reported cases in The Netherlands) [3]. One of these samples had been shown previously to be PCR-positive with a different pr ...
This article was published in an Elsevier journal. The attached copy
... were repeated every day for 7 days. Bioluminescence signal data from each sample was quantified by manually demarcating regions of interest and analyzed using LivingImage analysis software (Xenogen). 3. Results and discussion 3.1. Generation of VZV ORF deletion mutants In order to test this new VZVl ...
... were repeated every day for 7 days. Bioluminescence signal data from each sample was quantified by manually demarcating regions of interest and analyzed using LivingImage analysis software (Xenogen). 3. Results and discussion 3.1. Generation of VZV ORF deletion mutants In order to test this new VZVl ...
... SC-TR is a continuous, direct and highly specific assay of TR in cell lysates that makes use of the reduction of selenocystine (SC). SC is a small, commercially available diselenide-containing amino acid that can be reduced only by TR. The SC–TR assay can be either performed by standard spectrophoto ...
Effects of Natural Selection on Interpopulation Divergence
... at which the polymorphism occurs, which may also be referred to as a “SNP locus”; see Hughes et al. 2003) is equivalent to a low allelic frequency of the less common of the two alleles. The reduction of gene diversity at these SNP sites, in comparison to SNPs in the same genes not affecting protein ...
... at which the polymorphism occurs, which may also be referred to as a “SNP locus”; see Hughes et al. 2003) is equivalent to a low allelic frequency of the less common of the two alleles. The reduction of gene diversity at these SNP sites, in comparison to SNPs in the same genes not affecting protein ...
The pivotal roles of mitochondria in cancer: Warburg and beyond
... This propensity to metabolize glucose at excessive rates is the only requirement, and the basis behind the clinical imaging and staging of tumors via positron emission tomography (PET) [7,8]. Despite the universality of this technique, study of the “bioenergetic” basis behind this phenotype has not ...
... This propensity to metabolize glucose at excessive rates is the only requirement, and the basis behind the clinical imaging and staging of tumors via positron emission tomography (PET) [7,8]. Despite the universality of this technique, study of the “bioenergetic” basis behind this phenotype has not ...
Introduction to Sierra Molecular Page 1 INTRODUCTION TO
... Sierra Molecular's AssayAssure® products enable extraction of labile RNA and DNA from bacteria, viruses, and parasitic pathogens for diagnostic assays, even in extremely low target samples and highly inhibitory sample matrices. GeneLock® also preserve high quantity, high quality mRNA in whole blood, ...
... Sierra Molecular's AssayAssure® products enable extraction of labile RNA and DNA from bacteria, viruses, and parasitic pathogens for diagnostic assays, even in extremely low target samples and highly inhibitory sample matrices. GeneLock® also preserve high quantity, high quality mRNA in whole blood, ...
Assay Quality Considerations
... False Negative: ADO Allele drop-out (ADO): the failure of a molecular test to amplify or detect one or more alleles Potential causes: DNA template concentration • Incomplete cell lysis • DNA degradation Non-optimized assay conditions Unknown polymorphisms in target sites Reagent component ...
... False Negative: ADO Allele drop-out (ADO): the failure of a molecular test to amplify or detect one or more alleles Potential causes: DNA template concentration • Incomplete cell lysis • DNA degradation Non-optimized assay conditions Unknown polymorphisms in target sites Reagent component ...
En/Spm-LIKE TRANSPOSONS IN POACEAE SPECIES
... a given cereal’s nuclear genome) [1]. From the investigation of the Drosophila genomes, it is known that TE can give rise to significant genomic changes by creating mutations, altering gene expression, conducting telomeric function, and promoting chromosomal aberrations [2-4]. TE was divided into tw ...
... a given cereal’s nuclear genome) [1]. From the investigation of the Drosophila genomes, it is known that TE can give rise to significant genomic changes by creating mutations, altering gene expression, conducting telomeric function, and promoting chromosomal aberrations [2-4]. TE was divided into tw ...
Dell`Orphano: SNP discovery
... • 55 were discarded by computer analysis (filters). • The oligonucleotide selection program (osp) was used to design primers to amplify the 98 remaining SNPs. • 30 SNPs were in regions with no suitable amplimers. • In all, 44 STSs were developed to amplify the remaining 68 SNPs, with 16 STSs contain ...
... • 55 were discarded by computer analysis (filters). • The oligonucleotide selection program (osp) was used to design primers to amplify the 98 remaining SNPs. • 30 SNPs were in regions with no suitable amplimers. • In all, 44 STSs were developed to amplify the remaining 68 SNPs, with 16 STSs contain ...
Lecture slides
... As reference one can use an artificial “median array” for a set of arrays or use a log-normal distribution, which is a good approximation. ...
... As reference one can use an artificial “median array” for a set of arrays or use a log-normal distribution, which is a good approximation. ...
Assembly of microarrays for genome-wide measurement of
... Array printing. We used a custom built printer, employing a 4 x 4 array of quartz capillary tubes spaced on 3 mm centers to print ~70-100 μm diameter spots on 130 μm centers. We printed each DNA solution in triplicate to create an array of ~7500 elements in a 12 mm square area. We printed the arrays ...
... Array printing. We used a custom built printer, employing a 4 x 4 array of quartz capillary tubes spaced on 3 mm centers to print ~70-100 μm diameter spots on 130 μm centers. We printed each DNA solution in triplicate to create an array of ~7500 elements in a 12 mm square area. We printed the arrays ...
Microvolume Quantification of Nucleic Acids in Molecular Diagnostics
... than an hour. However, if failed DNA extractions are not identified, the delay while accumulating sufficient samples for analysis may delay the reporting of results by as much as 1 – 3 weeks in medium to small sized facilities. Applying this paradigm of nucleic acid quality control post-DNA extracti ...
... than an hour. However, if failed DNA extractions are not identified, the delay while accumulating sufficient samples for analysis may delay the reporting of results by as much as 1 – 3 weeks in medium to small sized facilities. Applying this paradigm of nucleic acid quality control post-DNA extracti ...
2008 BSHG newesletter 01
... In its current form DNA diagnostics is a very targeted process. For each sample only a very small section of the total DNA is analysed. The simplest way to achieve targeting with massively parallel sequencing is to analyse PCR products rather than whole DNA. This is not straightforward for a number ...
... In its current form DNA diagnostics is a very targeted process. For each sample only a very small section of the total DNA is analysed. The simplest way to achieve targeting with massively parallel sequencing is to analyse PCR products rather than whole DNA. This is not straightforward for a number ...
BC Yang
... substance of unknown function in the nuclei of human white blood cells. This substance will later be called deoxyribonucleic acid, or DNA. 1924 Microscope studies using stains for DNA and protein show that both substances are present in chromosomes. 1928 Franklin Griffith, a British medical offi ...
... substance of unknown function in the nuclei of human white blood cells. This substance will later be called deoxyribonucleic acid, or DNA. 1924 Microscope studies using stains for DNA and protein show that both substances are present in chromosomes. 1928 Franklin Griffith, a British medical offi ...
Functional Photonics for Single Bioentities a biophotonics Platform
... Metallic nanoparticles (NP) (from 100nm to 102 nm) can be used for nonfluorescent labels. Interaction with probe light is through light scattering, plasmon resonance or local enhancement of nonlinear optical response. We will functionalise a number of NP configurations (e.g. dots, shells, rods, ...) ...
... Metallic nanoparticles (NP) (from 100nm to 102 nm) can be used for nonfluorescent labels. Interaction with probe light is through light scattering, plasmon resonance or local enhancement of nonlinear optical response. We will functionalise a number of NP configurations (e.g. dots, shells, rods, ...) ...
Southern Analysis: - California State University San Marcos
... 0.1 M Tris (pH 7.5), 0.15 M NaCl 1 min 0.1 M Malate, 0.15 M NaCl,0.5% ...
... 0.1 M Tris (pH 7.5), 0.15 M NaCl 1 min 0.1 M Malate, 0.15 M NaCl,0.5% ...
Syllabus
... To gain a general overview, you will want to look at the texts used for MCB 104, or any other Genetics or Cell Biology course. These include Molecular Biology of the Cell, Alberts et al, 5th edition, Chapters 4, 5 or Genetics: From Genes to Genomes, Hartwell et al, 3rd edition, Chapters 4, 13, 14, 1 ...
... To gain a general overview, you will want to look at the texts used for MCB 104, or any other Genetics or Cell Biology course. These include Molecular Biology of the Cell, Alberts et al, 5th edition, Chapters 4, 5 or Genetics: From Genes to Genomes, Hartwell et al, 3rd edition, Chapters 4, 13, 14, 1 ...
ASE using Solexa Protocol
... of the SNP such that the base immediately following the 3’ end of the primer is the SNP, the second 200-300bp's downstream from the SNP. 3) Check primer design and verify that no additional SNP's occur within the gene specific annealing primers. 4) Create barcodes for each unique sample (we used 1-3 ...
... of the SNP such that the base immediately following the 3’ end of the primer is the SNP, the second 200-300bp's downstream from the SNP. 3) Check primer design and verify that no additional SNP's occur within the gene specific annealing primers. 4) Create barcodes for each unique sample (we used 1-3 ...
NEW LUNG CANCER TREATMENT POSSIBILITIES THROUGH MOLECULAR TUMOR TESTING
... is particularly recommended if you have the adenocarcinoma sub-type of NSCLC, though other sub-types can also contain these characteristics. Generally, if one of these tumor characteristics is present, the other is not. Clinical Trials - Even if your tumor is negative for EGFR and ALK mutations, you ...
... is particularly recommended if you have the adenocarcinoma sub-type of NSCLC, though other sub-types can also contain these characteristics. Generally, if one of these tumor characteristics is present, the other is not. Clinical Trials - Even if your tumor is negative for EGFR and ALK mutations, you ...
Fluorescence In Situ Hybridization in Cardiovascular Disease
... 10. Human Cot-1 DNA and total human placental DNA is used to suppress crosshybridization of large human DNA probes to the human repetitive DNA sequences. 11. Dextran sulfate used in the preparation of hybridization buffer is difficult to dissolve. After adding all ingredients, the preparation should ...
... 10. Human Cot-1 DNA and total human placental DNA is used to suppress crosshybridization of large human DNA probes to the human repetitive DNA sequences. 11. Dextran sulfate used in the preparation of hybridization buffer is difficult to dissolve. After adding all ingredients, the preparation should ...
technion - israel institute of technology - Technion
... DNA microarrays are a well-established technology for measuring gene expression levels. Currently, microarrays designed for this purpose are biased toward known and predicted gene structures, and use relatively few probes for each gene. With the ability to shrink feature size, new families of whole- ...
... DNA microarrays are a well-established technology for measuring gene expression levels. Currently, microarrays designed for this purpose are biased toward known and predicted gene structures, and use relatively few probes for each gene. With the ability to shrink feature size, new families of whole- ...
Virtual karyotype
Virtual karyotype is the digital information reflecting a karyotype, resulting from the analysis of short sequences of DNA from specific loci all over the genome, which are isolated and enumerated. It detects genomic copy number variations at a higher resolution level than conventional karyotyping or chromosome-based comparative genomic hybridization (CGH). The main methods used for creating virtual karyotypes are array-comparative genomic hybridization and SNP arrays.