Information S1.
... distribution were centralized in one laboratory (UMR775, INSERM, Pr P. Laurent-Puig). The p.G12R cell line obtained by direct mutagenesis has a mismatch located on codon 28 (c.84 T>C). It was subsequently shown that laboratories that did not correctly genotype the p.G12R had chosen their upper prime ...
... distribution were centralized in one laboratory (UMR775, INSERM, Pr P. Laurent-Puig). The p.G12R cell line obtained by direct mutagenesis has a mismatch located on codon 28 (c.84 T>C). It was subsequently shown that laboratories that did not correctly genotype the p.G12R had chosen their upper prime ...
Gene Section GGH (gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) Atlas of Genetics and Cytogenetics
... cells in patients with a wildtype germline GGH genotype, and methylation of entire GGH promoter region (seen in leukemia cells from approximately 15% of patients with nonhyperdiploid B-lineage ALL) is associated with significantly reduced GGH mRNA expression and catalytic activity and with significa ...
... cells in patients with a wildtype germline GGH genotype, and methylation of entire GGH promoter region (seen in leukemia cells from approximately 15% of patients with nonhyperdiploid B-lineage ALL) is associated with significantly reduced GGH mRNA expression and catalytic activity and with significa ...
New Potential Lung Cancer Biomarkers Identified
... the biomolecular changes occurring within residual tumor cells under precision treatment with ALK-specific kinase inhibitor treatments.” “Using Protea’s MSI technology, our team discovered several metabolites that were changing over a time course of treatment. Peptides were detected that showed diff ...
... the biomolecular changes occurring within residual tumor cells under precision treatment with ALK-specific kinase inhibitor treatments.” “Using Protea’s MSI technology, our team discovered several metabolites that were changing over a time course of treatment. Peptides were detected that showed diff ...
Gene Section PML (Promyelocytic leukemia) Atlas of Genetics and Cytogenetics
... In a wide variety of tissues. In hematopoietic tissue, expression apparently restricted to myeloid precursors. ...
... In a wide variety of tissues. In hematopoietic tissue, expression apparently restricted to myeloid precursors. ...
Gene Section SEMA3B (sema domain, immunoglobulin domain 3B)
... (NPC), as evident from both loss of heterozygosity analysis and functional studies. 21 primary NPC tumors and 2 NPC cell lines (CNE2 and SUNE1) screened for mutations by PCR-sequencing and two missense polymorphisms including Thr415Ile and lle242Met were found in SEMA3B. For the Thr415Ile polymorphi ...
... (NPC), as evident from both loss of heterozygosity analysis and functional studies. 21 primary NPC tumors and 2 NPC cell lines (CNE2 and SUNE1) screened for mutations by PCR-sequencing and two missense polymorphisms including Thr415Ile and lle242Met were found in SEMA3B. For the Thr415Ile polymorphi ...
122.1 Schramm
... patterns differentially expressed between subgroups. Surprisingly, expression profiles of stage 4 and stage 4s neuroblastomas could not clearly be distinguished by any of the mathematical methods applied. In contrast, MYCN-amplification as well as high expression of the neurotrophin receptor TrkA de ...
... patterns differentially expressed between subgroups. Surprisingly, expression profiles of stage 4 and stage 4s neuroblastomas could not clearly be distinguished by any of the mathematical methods applied. In contrast, MYCN-amplification as well as high expression of the neurotrophin receptor TrkA de ...
S1 Text. Supplementary Methods
... Finally, we filtered all genomic regions containing a high proportion of heterozygous genotype calls in the C. rubella line Cr1GR1 which has been inbred in the lab for at least six generations. This was also done using the cumulative distribution of the fraction of heterozygous calls across the geno ...
... Finally, we filtered all genomic regions containing a high proportion of heterozygous genotype calls in the C. rubella line Cr1GR1 which has been inbred in the lab for at least six generations. This was also done using the cumulative distribution of the fraction of heterozygous calls across the geno ...
Gene Section CCDC6 (coiled-coil domain containing 6) Atlas of Genetics and Cytogenetics
... tyrosine kinase domain of RET. The fusion is a constitutively active tyrosine kinase. Oncogenesis In transgenic mice the fusion gave rise to mammary adenocarcinomas and, less frequently, hyperplasia of sebaceous glands and rare benign skin tumors. ...
... tyrosine kinase domain of RET. The fusion is a constitutively active tyrosine kinase. Oncogenesis In transgenic mice the fusion gave rise to mammary adenocarcinomas and, less frequently, hyperplasia of sebaceous glands and rare benign skin tumors. ...
Mutation identification by whole genome sequencing
... 2. Next Generation Sequencing by the Illumina method a. Completed in a flow cell. 8 lanes on each cell can produce 12 billion bases of sequence information b. Genomic DNA is fragmented, bound to the surface of the flow cell, and each fragment amplified to produce a cluster of identical fragments. c. ...
... 2. Next Generation Sequencing by the Illumina method a. Completed in a flow cell. 8 lanes on each cell can produce 12 billion bases of sequence information b. Genomic DNA is fragmented, bound to the surface of the flow cell, and each fragment amplified to produce a cluster of identical fragments. c. ...
Additional file 5
... (A-C) Results from hybridization of Bp K96243 against Bp 22 genomic DNA (intra-species). Both BpK96243 and Bp22 genomic DNA were hybridized onto the pan genome microarray in a dual channel experiment. A) Hybridization signals associated with BpK96243 (left) and Bp22 (right) genomic DNA. Raw flourese ...
... (A-C) Results from hybridization of Bp K96243 against Bp 22 genomic DNA (intra-species). Both BpK96243 and Bp22 genomic DNA were hybridized onto the pan genome microarray in a dual channel experiment. A) Hybridization signals associated with BpK96243 (left) and Bp22 (right) genomic DNA. Raw flourese ...
High-Resolution Array-Based Comparative Genomic Hybridization
... these lesions are submitted entirely in formalin for histologic analysis by conventional hematoxylin and eosin–stained sections, and freshfrozen material for ancillary studies is rarely collected. Molecular techniques, such as comparative genomic hybridization (CGH), can detect chromosomal alteratio ...
... these lesions are submitted entirely in formalin for histologic analysis by conventional hematoxylin and eosin–stained sections, and freshfrozen material for ancillary studies is rarely collected. Molecular techniques, such as comparative genomic hybridization (CGH), can detect chromosomal alteratio ...
Statistical Analyses of Microarray Data
... Oligo Array Image Analysis • About 100 pixels per probe cell • These intensities are combined to form one ...
... Oligo Array Image Analysis • About 100 pixels per probe cell • These intensities are combined to form one ...
Microarray - Clemson University
... global expression analysis that is not feasible with conventional techniques ...
... global expression analysis that is not feasible with conventional techniques ...
Genomic analysis of metastasis reveals an essential role for RhoC
... Test: Use a retrovirus vector to introduce RhoC into low metastatic cell line or RhoC dominant negative form into highly metastatic cell line ...
... Test: Use a retrovirus vector to introduce RhoC into low metastatic cell line or RhoC dominant negative form into highly metastatic cell line ...
Gene Section POU6F2 (POU domain, class 6, transcription factor 2)
... In a patient with WT and LOH at chromosome 7p14, a germline 552G-T transversion in exon 5 of the POU6F2 gene, resulting in a gln184-to-his (Q184H) substitution in a glutamine repeat domain, was identified. The patient showed loss of the constitutionally wildtype allele in tumor DNA. Neither the moth ...
... In a patient with WT and LOH at chromosome 7p14, a germline 552G-T transversion in exon 5 of the POU6F2 gene, resulting in a gln184-to-his (Q184H) substitution in a glutamine repeat domain, was identified. The patient showed loss of the constitutionally wildtype allele in tumor DNA. Neither the moth ...
HiPerDART Targets and Objectives
... every tumor contains informative gene expression signatures that, at the time of diagnosis, can direct the biologic behaviour over time. Recently, several microarray gene expression profiles have shown promise in predicting the prognosis of stage II and III colon cancer (Eschrich et al, 2005; Barrie ...
... every tumor contains informative gene expression signatures that, at the time of diagnosis, can direct the biologic behaviour over time. Recently, several microarray gene expression profiles have shown promise in predicting the prognosis of stage II and III colon cancer (Eschrich et al, 2005; Barrie ...
Virtual karyotype
Virtual karyotype is the digital information reflecting a karyotype, resulting from the analysis of short sequences of DNA from specific loci all over the genome, which are isolated and enumerated. It detects genomic copy number variations at a higher resolution level than conventional karyotyping or chromosome-based comparative genomic hybridization (CGH). The main methods used for creating virtual karyotypes are array-comparative genomic hybridization and SNP arrays.