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Guidance for Working with Viral Vectors
Guidance for Working with Viral Vectors

... Pseudorabies virus (PRV) is a herpesvirus of swine and the etiological agent of Aujeszky's disease. PRV is a double-stranded DNA, enveloped virus with icosahedral symmetry. It is classified as a member of the subfamily Alphaherpesvirinae within the family Herpesviridae. Characteristics of this subfa ...
Notes Packet - Ms. Ottolini`s Biology Wiki!
Notes Packet - Ms. Ottolini`s Biology Wiki!

... Before scientists knew that DNA replication was semi-conservative, they hypothesized that it could be __________________________________. If this was the case, each parent DNA strand would still be used to build a daughter DNA strand. However, after replication was complete, the two parent DNA stran ...
Viruses and Bacteria - Hartland High School
Viruses and Bacteria - Hartland High School

... acid, either RNA or DNA, and an outer protein coat called a capsid. Some relatively large viruses, such as human flu viruses, may have an additional layer, called an envelope, surrounding their capsids. Envelopes are composed primarily of the same materials found in the plasma membranes of all cells ...
Viruses and Bacteria
Viruses and Bacteria

... acid, either RNA or DNA, and an outer protein coat called a capsid. Some relatively large viruses, such as human flu viruses, may have an additional layer, called an envelope, surrounding their capsids. Envelopes are composed primarily of the same materials found in the plasma membranes of all cells ...
Unit 8 Molecular Genetics Chp 16 DNA PPT
Unit 8 Molecular Genetics Chp 16 DNA PPT

... • Further evidence that DNA was the genetic material was derived from studies that tracked the infection of bacteria by viruses. • Viruses consist of a DNA (sometimes RNA) enclosed by a protective coat of protein. • To replicate, a virus infects a host cell and takes over the cell’s metabolic machi ...
Chapter06_Outline
Chapter06_Outline

... DNA Structure • The nucleotides are joined to form a polynucleotide chain, in which the phosphate attached to the 5' carbon of one sugar is linked to the hydroxyl group attached to the 3' carbon of the next sugar in line • The chemical bonds by which the sugar components of adjacent nucleotides are ...
Estimation of Circular DNA Size Using r
Estimation of Circular DNA Size Using r

... with appropriate linear DNA size markers. In this report several parameters important in the application of this method are examined. The effects of yirradiation on DNA have been extensively studied (summarized in (10)). y-Irradiation introduces a variety of lesions into DNA, including both single- ...
The DNA repair helicase UvrD is essential for replication
The DNA repair helicase UvrD is essential for replication

... degradation ...
power pack 5 dna replication
power pack 5 dna replication

... 3. Okasaki segments are small pieces of DNA containing 1000 to 1500 nucleotides and are formed in a. lagging strand b. leading strand c. sense strand d. non sense strand 4. The area of unwinding and separation of DNA strands during replication is called a. origin b. initiation point c. primer d. rep ...
African green monkeys - Journal of General Virology
African green monkeys - Journal of General Virology

... groups have been proposed to classify primate lentiviruses (reviewed in Sharp et al., 1994): (i) HIV-t and SIVcpz from chimpanzees, (ii) HIV-2 and SIVsm from sooty mangabeys, (iii) SIVmnd from mandrills (Papio sphinx), (iv) SIVagm from African green monkeys (Cereopithecus aethiops) (AGMs) and (v) SI ...
African green monkeys (Cercopithecus aethiops sabaeus) in the wild
African green monkeys (Cercopithecus aethiops sabaeus) in the wild

... groups have been proposed to classify primate lentiviruses (reviewed in Sharp et al., 1994): (i) HIV-t and SIVcpz from chimpanzees, (ii) HIV-2 and SIVsm from sooty mangabeys, (iii) SIVmnd from mandrills (Papio sphinx), (iv) SIVagm from African green monkeys (Cereopithecus aethiops) (AGMs) and (v) SI ...
CHAPTER  1 LITERATURE  SURVEY
CHAPTER 1 LITERATURE SURVEY

... transcribed from plasmids) containing the viral genome directly into cells, as was first demonstrated with Poliovirus (PV; Racaniello & Baltimore 1981). Due to their generally smaller genome sizes compared to DNA viruses, whole RNA virus genomes can be cloned as cDNA and manipulated at will. This ap ...
Gene sequences useful for predicting relatedness of whole
Gene sequences useful for predicting relatedness of whole

... identified (Table 1). It was known that the genera Escherichia, Salmonella and Shigella were highly related (Brenner et al., 1969, 1972; Crosa et al., 1973), so they were treated as if they belonged to a single genus. Genome sequences within each of the 16 groups were aligned in pairwise fashion by ...
File - Central Dogma of Molecular Biology
File - Central Dogma of Molecular Biology

... they determined from the work of Franklin and explained Chargaff’s findings. • They explained the base paring rules, the shape and the width of the DNA and showed that none of this was dependent on the sequence of the nucleotides. • Thus, the DNA could be put together an infinite number of ways. ...
CHAPTER 16 - HCC Learning Web
CHAPTER 16 - HCC Learning Web

... • In 1952, Alfred Hershey and Martha Chase performed experiments showing that DNA is the genetic material of a phage known as T2 • To determine this, they designed an experiment showing that only one of the two components of T2 (DNA or protein) enters an E. coli cell during infection • They conclud ...
PPT2 - Ycmou
PPT2 - Ycmou

...  Helicases mainly functions in separating the two annealed nucleic acid strands (DNA, RNA).  For the replication, it is necessary to separate the double stranded DNA strand which is done by Helicases.  For this activity they derived energy from ATP or GTP hydrolysis which is the output of breakin ...
the smallest dinoflagellate genome is yet to be found: a comment on
the smallest dinoflagellate genome is yet to be found: a comment on

... generalize in their conclusion, their report poses two challenges for dinoflagellate researchers; i.e. to look into the evolution of dinoflagellate genomes and to look for the smallest dinoflagellate genome. What makes the genome of Symbiodinium so ‘‘small’’ and that of some other dinoflagellates so ...
incidence and detection of aviadenoviruses of serotypes 1 and 5 in
incidence and detection of aviadenoviruses of serotypes 1 and 5 in

... molecules and are responsible for the trimer formation. The highly variable domains are placed mainly outside of the virion and are responsible for antigenic variation of adenovirus (5, 11, 15). Aviadenoviruses are divided into three groups. Group I is additionally divided in to five subgroups (AE), ...
Conformationally Complex Epitope on Glycoprotein H
Conformationally Complex Epitope on Glycoprotein H

... member of the subset of conserved herpesvirus genes. As determined by comparisons of encoded amino acid sequences, it is apparent that the homologous gH sequences from different herpesvirus subgroups (a, or -y) form one of the most divergent of these related protein families (15, 20, 21). To further ...
FILLED BY VECTOR CORE PROJECT: RECEIVED: LOT
FILLED BY VECTOR CORE PROJECT: RECEIVED: LOT

... The materials produced by the BCK Virus Vector Laboratory require the following levels of containment: BSL1: plasmids and virus vectors tested free of replication competent virus (upon specific request) BSL2: virus vectors upon standard request if the inserted genetic material does not increase the ...
Nanomechanical Devices Based on DNA
Nanomechanical Devices Based on DNA

... Mg2‡ ions was used to trigger a supercoiling of two DNA strands within supramolecular aggreates comprised of biotinylated DNA and streptavidin (STV).[13] The twisting of the DNA strands leads to a decrease in the distance between adjacent STV particles (Figure 1 B). Potential applications of such st ...
Types of variation in DNA-A among isolates of East African cassava
Types of variation in DNA-A among isolates of East African cassava

... cotton and okra in Pakistan (Zhou et al., 1998). If the same is true for EACMV, the extent of the sequence dissimilarity between type 1 and type 2 isolates in the intergenic region would be expected to be different on either side of ori. Inspection of the intergenic region sequences shows that, to t ...
RECESSIVE ALLELES FOUND AT R AND C LOCI IN
RECESSIVE ALLELES FOUND AT R AND C LOCI IN

... last line. No evidence of recessive alleles at a2 or c2 loci were obtained in these tests. Few successful crosses were obtained with the a2 tester. The presence of recessive alleles at two or three loci when only one was expected does much to explain the aberrant ratio phenomenon. Crosses between pl ...
Individual eukaryotic genomes
Individual eukaryotic genomes

... the mouse Mus musculus M. musculus is the second mammal to have its genome sequenced. Mouse diverged from human 75 MYA. Distinguishing features: only 300 of 30,000 annotated genes have no human orthologs Genome size: 2.5 Gb (euchromatic portion)(cf. 2.9 Gb human) Chromosomes: 6 Genes: about 30,000 W ...
Nucleotide sequences of the trailer, nucleocapsid protein gene and
Nucleotide sequences of the trailer, nucleocapsid protein gene and

... elements not being a factor in virulence. Together with previously published data, this report completes the sequence of the 15 186 nt genomic RNA of NDV strain Beaudette C. ...
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DNA virus



A DNA virus is a virus that has DNA as its genetic material and replicates using a DNA-dependent DNA polymerase. The nucleic acid is usually double-stranded DNA (dsDNA) but may also be single-stranded DNA (ssDNA). DNA viruses belong to either Group I or Group II of the Baltimore classification system for viruses. Single-stranded DNA is usually expanded to double-stranded in infected cells. Although Group VII viruses such as hepatitis B contain a DNA genome, they are not considered DNA viruses according to the Baltimore classification, but rather reverse transcribing viruses because they replicate through an RNA intermediate. Notable diseases like smallpox, herpes, and chickenpox are caused by such DNA viruses.
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