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Bioinformatics
Bioinformatics

... The sequencing of the human genome and many other genomes heralds a new age in human biology, offering unprecedented opportunities to improve human health and to stimulate industrial and economic activity. In making its contribution to realising these benefits, this theme will focus on integrating p ...
Mutation - NIU Department of Biological Sciences
Mutation - NIU Department of Biological Sciences

... Mutations can be classified according to their effects on the protein (or mRNA) produced by the gene that is mutated. 1. Silent mutations (synonymous mutations). Since the genetic code is degenerate, several codons produce the same amino acid. Especially, third base changes often have no effect on t ...
Ethical issues in personalized genomics
Ethical issues in personalized genomics

... • Most hunters and gatherers would never have been able to wrap their heads around the concept of a supermarket. The naysayers would have insisted that landing food was always going to remain probabilistic, that no amount of technology would ever make the satisfaction of hunger anything more than a ...
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Concepts of Biology
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... The first cloned agricultural animal was Dolly, a sheep who was born in 1996. The success rate of reproductive cloning at the time was very low. Dolly lived for six years and died of a lung tumor (Figure 10.7). There was speculation that because the cell DNA that gave rise to Dolly came from an olde ...
Cryptography and Linguistics of Macromolecules Cryptography and
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CHAPTER 19: GENE TECHNOLOGY
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Linkage Analysis - The Blavatnik School of Computer Science

... The Main Idea/usage: Neighboring genes on the chromosome have a tendency to stick together when passed on to offsprings. Therefore, if some disease is often passed to offsprings along with specific markergenes , then it can be concluded that the gene(s) which are responsible for the disease are loc ...
lec36_2013 - Andrew.cmu.edu
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DNA and RNA

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Student Handout - University of California, Irvine
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... complete encyclopedia. If you were missing a single volume of an encyclopedia, you could be missing information you might need some time in the future. This is also true for our chromosomes. One chromosome contains only part of the instructions for making a human. All of our nuclei, except for those ...
Heredity and Meiosis - Chaparral Star Academy
Heredity and Meiosis - Chaparral Star Academy

...  DNA can store huge amounts of information because it is very long.  Since there are only 2 possible choices of how nucleotides (A-T) (C-G) will match up, DNA sequences the information in different orders to code for different genes  Can use these sequences to check for evolutionary relationshi ...
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... • Siamese cats have a form of albinism resulting from a mutation carried genetically on the C-locus-- the same locus that also houses the gene for complete albinism. Temperature-sensitive albino cats have a mutated form of tyrosinase, the enzyme resposible for producing melanin, the pigment involve ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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