• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
How many chromosomes are shown in a normal human karyotype?
How many chromosomes are shown in a normal human karyotype?

... determined to be part of the same DNA sequence. Notice that the fragments are single stranded. Determine the single-stranded DNA sequence that the fragments below are part of. Explain your ...
Biology 11.1 Gene Technology
Biology 11.1 Gene Technology

... interest into another cell. Commonly used vectors include viruses, yeast, and plasmids. Plasmids are circular DNA molecules that can replicate independently of the main chromosomes of the bacteria. ...
Chapters 10a and 11 PowerPoint
Chapters 10a and 11 PowerPoint

... The failure of homologous chromosomes to separate properly during meiosis is called nondisjunction In one type of nondisjunction, two kinds of gametes ...
Slide 1
Slide 1

... • Mitochondria and chloroplasts, the principal energy-producing organelles of eukaryotes, arose from symbiotic association of prokaryotes of the domain Bacteria within eukaryotic cells, • The process is called endosymbiosis. • Assuming that an RNA world existed, selfreplicating entities have populat ...
notes_14C_nucacids
notes_14C_nucacids

... - Garrod – Studied rare genetic disorder: Alkaptonuria; concluded that specific gene is associated with absence of a specific enzyme. ...
Nucleotides and Nucleic Acids
Nucleotides and Nucleic Acids

... - Garrod – Studied rare genetic disorder: Alkaptonuria; concluded that specific gene is associated with absence of a specific enzyme. ...
iMap Exercise ()
iMap Exercise ()

... PCO085026_ov in the marker area. Click on it. The marker should be highlighted in blue and BACs hit by the marker should be in green. • Click on Zoom in to enlarge the BAC display. • Which of these BACs would be good candidates for sequencing? (Hint: pick the longest BACs.) ...
New techniques and the GMO-legislation
New techniques and the GMO-legislation

... Techniques/methods of genetic modification yielding organisms to be excluded from the Directive, on the condition that they do not involve the use of recombinant nucleic acid molecules or genetically modified organisms other than those produced by one or more of the techniques/methods listed below a ...
DNA
DNA

... • Construct and identify the parts of a model of a DNA molecule. • Describe how DNA copies itself. PA Science and Technology Standards covered in this unit: • 3.1.7B Describe the use of models as an application of scientific or technological concepts. • 3.3.7C Know that every organism has a set of g ...
Slide 1
Slide 1

... • If the prevalence of disease is greater in one sub-population, then this group will be over-represented amongst the cases. • Any marker which is also of higher frequency in that subgroup will appear to be associated with the disease ...
Topic 5
Topic 5

... disequilibrium” (here, the sharing of DNA sequences neighboring the CF allele that have been coinherited for many generations). Linkage disequilibrium is only seen for DNA very close to the mutant gene and can therefore help to localize the disease gene. There are important mapping approaches that r ...
Final Review
Final Review

... DNA and RNA molecules have structural similarities and differences that define function. ● Both have three components — sugar, phosphate and a nitrogenous base — which form nucleotide units that are connected by covalent bonds to form a linear molecule with 3' and 5' ends, with the nitrogenous bases ...
C1. At the molecular level, sister chromatid exchange and
C1. At the molecular level, sister chromatid exchange and

... C15. First, gene rearrangement of V, D, and J domains occurs within the light- and heavy-chain genes. Second, within a given B cell, different combinations of light and heavy chains are possible. And third, imprecise fusion may occur between the V, D, and J domains. C16. The function of the RAG1 an ...
history of genetics
history of genetics

... chromosomes separate, with one of each of the sister chromatids going to opposite poles of the cell. Therefore, each new cell has the correct number of chromosomes. Cell division during which sister chromatids fail to ...
Sequencing
Sequencing

Schedule of Lecture and Laboratory Sessions
Schedule of Lecture and Laboratory Sessions

... 10. To discuss the use of nutritional mutants (auxotrophs) in the study of bacterial conjugation 11. To describe parasexual mating (conjugation) between F+ and F- bacteria 12. To explain the F factor, what it encodes, and the mechanism of transfer from F+ to F13. Describe Hfr strains and interrupted ...
Spring 2012 Agriscience Midterm Name (print large and clearly
Spring 2012 Agriscience Midterm Name (print large and clearly

... Treat each question like a short answer question if you can. Cover the answers, create an answer in your head, and then match that answer to those given to find the best answer. If you do not know an answer, skip it and come back later. Use information from other questions to help you. One question ...
What is DNA Computing?
What is DNA Computing?

... chemically interact according to defined rules to produce new molecules Laboratory techniques that allow the isolation/identification of product molecules with specific properties  PCR, Ligation, Gel Electrophoresis, etc. ...
Drosophila genome takes flight
Drosophila genome takes flight

... for the ‘shotgun’ sequencing of large eukaryotic genomes, which relies on breaking a genome into small random pieces that are then sequenced and reassembled by computational methods. This strategy, which had been successfully used on small prokaryotic genomes, met with mixed responses from the scien ...
BB30055: Genes and genomes
BB30055: Genes and genomes

... design PCR primers unique to one locus in the genome .a single pair of PCR primers will produce different sized products for each of the different length microsatellites ...
Genetics Summary
Genetics Summary

... - Restriction enzymes —> find certain codons and cut at that specific site - Proteome —> complete set of coded proteins in a genome - Human genome has ~ 3.2 billion bases —> 32,000 bases actually code for something - Splicing —> this uses a combination of a set of genes, resulting in bit variety fro ...
Lecture 2: Functional analysis of Arabidopsis
Lecture 2: Functional analysis of Arabidopsis

... Mapping an Arabidopsis mutation using molecular markers Mutations identified in Arabidopsis thaliana (Columbia). Another frequently used accession is Arabidopsis thaliana (Landsberg erecta). Can use DNA markers to distinguish between Landsberg and Columbia. There are more than 50,000 sequence polym ...
The ABC`s of DNA - High Point University
The ABC`s of DNA - High Point University

... creations. "In fact," he writes, "with 30,000 genes, each directly interacting with four or five others on average, the human genome is not significantly more complex than a modern jet airplane, which contains more than 200,000 unique parts, each of them interacting with three or four others on aver ...
Biology DNA MCAS questions
Biology DNA MCAS questions

Apresentação do PowerPoint
Apresentação do PowerPoint

... Definition of genetic markers of the strains causative of different clinical manifestations of Chagas Disease Application: Prognosis and potential targets for treatment Trypanosoma cruzi invading a mammalian cell ...
< 1 ... 308 309 310 311 312 313 314 315 316 ... 561 >

Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report