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- Nour Al Maaref International School
- Nour Al Maaref International School

... _____ 1. Which conclusion is a result of Thomas Hunt Morgan’s research with fruit flies? a. Chromosome assortment during meiosis is not random. b. Linked genes are located on the same chromosome. c. The physical distance between genes can be determined. d. Genes recombine independently during mitosi ...
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... Total genomic DNA was isolated using GeneElute genomic DNA isolation kit (Sigma, USA) as per the manufacturer’s instructions and used as a template for PCR. Each reaction mixture contain approximately 10 ng of DNA; 2.5 mm MgCl2, 1x PCR buffer (Genei, Banglore, India), 200 μM each dCTP, dGTP, dATP an ...
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10.6AC The Pattern - Texarkana Independent School District

... 1. On rare occasion, codons are inserted, deleted, or substituted for one another during the process of DNA replication. Biologists identify these changes in DNA molecules as ___ (a) transferences. (b) mutations. (c) replications. (d) natural selection. 2. A biology teacher lists and explains some o ...
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... The detection of a dimeric form of the replicative intermediate, a head-to-head concatemer of two covalently linked doublestranded forms- has led to a model in which the growing DNA strand replicates back on itself to produce a tetrameric form from which two complete positive or negative strands are ...
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... • 1. Is a chicken egg an organism, organ, or cell? • 2. What function do you think the yolk serves? • 3. Do chickens reproduce sexually (DNA is received from both parents) or asexually (only one parent is involved, and the offspring is genetically identical to the parent)? ...
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... matches, 555 (88%) of which were assigned a biological function (see Table 3 and Fig. 5, which are published as supporting information on the PNAS web site). The genome contains 583 protein-coding genes, with an average size of 1,007 nucleotides per gene and 42 RNA-specifying genes (three ribosomal ...
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`Natural selection merely modified while redundancy created

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... A very important class of structural-variant polymophisms involve copy number variants or CNVs. A copy number variant is simply a large (from1,000 to several million nucleotides) insertion, deletion, inversion, duplication or transposition. In short, it is a large structural variant. It had long bee ...
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Molecular biology is the branch of biology that deals with the

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... Chromosomes, Genes, and DNA • Genes carried by chromosomes are the units of inheritance that are sequences of DNA • The sequence of bases along a strand constitutes the genetic code – The genetic code gives instructions to perform a specific function in the body (i.e. release this protein or that h ...
Nerve activates contraction
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... • Gene cloning and genetic engineering were made possible by the discovery of restriction enzymes that cut DNA molecules at specific locations. • In nature, bacteria use restriction enzymes to cut foreign DNA, such as from phages or other bacteria. • Most restrictions enzymes are very specific, reco ...
Using Yeast to study Eukaryotic Gene Function From Recombinant
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... Cloning genes required for mating reveals a signaling pathway similar to that seen in higher organisms ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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