Supplementary Methods
... temperature for 10 min and the beads were washed as per the manufacturer’s instructions. The fragmented RNA samples were eluted in 10 μl 10 mM Tris-HCL (pH 7.5). For sscDNA generation, each sample was mixed with 2 μl 500 μM random primer (5’phosphate-N7-OH-3’; Integrated DNA Technologies, Coralvill ...
... temperature for 10 min and the beads were washed as per the manufacturer’s instructions. The fragmented RNA samples were eluted in 10 μl 10 mM Tris-HCL (pH 7.5). For sscDNA generation, each sample was mixed with 2 μl 500 μM random primer (5’phosphate-N7-OH-3’; Integrated DNA Technologies, Coralvill ...
polymorphism
... the Alu type of transposons amounts to approximately 10% of human DNA. Exactly where in a chromosome a transposable element inserts itself could be of great consequence. To see how, one needs to know that most of the 40,000 plus human genes code for proteins. Whether a protein is an enzyme, a transp ...
... the Alu type of transposons amounts to approximately 10% of human DNA. Exactly where in a chromosome a transposable element inserts itself could be of great consequence. To see how, one needs to know that most of the 40,000 plus human genes code for proteins. Whether a protein is an enzyme, a transp ...
ANSWER: Trp+
... b. Do the sites function in cis or in trans (or both)? oriT sites can function in both cis and trans. The site is the important for nickase protein recognition. OriV sites only work in cis because they are the sites for initiation of DNA synthesis. c. E. coli mutants that have a temperature sensitiv ...
... b. Do the sites function in cis or in trans (or both)? oriT sites can function in both cis and trans. The site is the important for nickase protein recognition. OriV sites only work in cis because they are the sites for initiation of DNA synthesis. c. E. coli mutants that have a temperature sensitiv ...
Dna rEPLICATION - Manning`s Science
... Details about the lagging strand: The 3’ to 5’ parent strand is a problem for DNA polymerase since it must synthesize in the 5’ 3’ direction Short RNA primer sequences of 10-60 RNA bases are bonded to regions of the lagging strand with the purpose of initiating DNA replication PRIMASE – an en ...
... Details about the lagging strand: The 3’ to 5’ parent strand is a problem for DNA polymerase since it must synthesize in the 5’ 3’ direction Short RNA primer sequences of 10-60 RNA bases are bonded to regions of the lagging strand with the purpose of initiating DNA replication PRIMASE – an en ...
practice exam 3_answer key
... c. Each ribosome has two binding sites for tRNA molecules. d. Ribosomes are not functional until both subunits are together e. The ribosome is the where protein modification and packaging occur 53. A physical or chemical agent that changes the nucleotide sequence of DNA is called a(n) a. terminator. ...
... c. Each ribosome has two binding sites for tRNA molecules. d. Ribosomes are not functional until both subunits are together e. The ribosome is the where protein modification and packaging occur 53. A physical or chemical agent that changes the nucleotide sequence of DNA is called a(n) a. terminator. ...
Chapter 8: Cell Division
... 11.What are the sex chromosomes versus the autosomal chromosomes? Understand how sex is determined in mammals and the structural difference between the X and Y chromosome. a. Know that the sex chromosomes are one set of chromosomes that determine sex of an individual; X,X = female and X,Y= male b. ...
... 11.What are the sex chromosomes versus the autosomal chromosomes? Understand how sex is determined in mammals and the structural difference between the X and Y chromosome. a. Know that the sex chromosomes are one set of chromosomes that determine sex of an individual; X,X = female and X,Y= male b. ...
Final Exam Bio 101 Sp08
... b. chopping up DNA using electric current in gelatin c. separating DNA fragments in a gelatin-like slab under and electric current d. duplicating a single DNA piece into thousands of copies e. creating mutations in genes that create gelatin protein 39. Polymerase chain reaction refers to the process ...
... b. chopping up DNA using electric current in gelatin c. separating DNA fragments in a gelatin-like slab under and electric current d. duplicating a single DNA piece into thousands of copies e. creating mutations in genes that create gelatin protein 39. Polymerase chain reaction refers to the process ...
Name: Date: Period:___ Midterm Review: Study Guide # 4 TOPICS
... Gene – piece of a chromosome that controls a trait Allele – different forms of a gene Dominant – a trait that always shows up when it is present Recessive – a trait that is hidden by a dominant one Homozygous – two of the same alleles Heterozygous – two different alleles ...
... Gene – piece of a chromosome that controls a trait Allele – different forms of a gene Dominant – a trait that always shows up when it is present Recessive – a trait that is hidden by a dominant one Homozygous – two of the same alleles Heterozygous – two different alleles ...
Activity--Extracting DNA - e
... use DNA to solve crimes and identify victims. Anthropological studies use DNA to help identify how different animals may have been related. Scientists can even determine why the famous chemist, John Dalton, was colorblind by analyzing his preserved eyeballs. But before scientists can analyze the DNA ...
... use DNA to solve crimes and identify victims. Anthropological studies use DNA to help identify how different animals may have been related. Scientists can even determine why the famous chemist, John Dalton, was colorblind by analyzing his preserved eyeballs. But before scientists can analyze the DNA ...
Part I: Multiple Choice ______1. A haploid cell is a cell a. in which
... ______1. A haploid cell is a cell a. in which the genes are arranged haphazardly. b. containing only one copy of each chromosome. c. that has resulted from the process of mitosis. d. with twice the number of chromosomes of a diploid cell. ______2. The members of a homologous pair of chromosomes a. a ...
... ______1. A haploid cell is a cell a. in which the genes are arranged haphazardly. b. containing only one copy of each chromosome. c. that has resulted from the process of mitosis. d. with twice the number of chromosomes of a diploid cell. ______2. The members of a homologous pair of chromosomes a. a ...
Transposable Elements
... terminal repeats ; usually the two copies of the repeat are closely related rather than ...
... terminal repeats ; usually the two copies of the repeat are closely related rather than ...
Mendel`s Laws and Genetics Quiz
... Mendel’s Laws and Genetics Quiz 1. The two versions of a gene for a characteristic are called a) genotypes. b) phenotypes. c) alleles. d) chromosomes. ...
... Mendel’s Laws and Genetics Quiz 1. The two versions of a gene for a characteristic are called a) genotypes. b) phenotypes. c) alleles. d) chromosomes. ...
BioSc 231 Exam 4 2008
... 12) _____ E. coli cells were spread on an agar plate, producing 1000 colonies. The colonies are replica plated on four agar plates containing 10 micrograms per milliliter of the antibiotic tetracycline and one agar plate without antibiotic. All of the colonies are able to grow on the agar plate with ...
... 12) _____ E. coli cells were spread on an agar plate, producing 1000 colonies. The colonies are replica plated on four agar plates containing 10 micrograms per milliliter of the antibiotic tetracycline and one agar plate without antibiotic. All of the colonies are able to grow on the agar plate with ...
The Effects of Plasmid on Genotype and Phenotype
... you can readily appreciate how this type of gene can cause serious medical problems when it occurs in pathogenic bacteria. For this reason, the plasmids such as pUC 18 which are used in recombinant DNA experiments were designed so that they cannot be exchanged with other bacteria except by special t ...
... you can readily appreciate how this type of gene can cause serious medical problems when it occurs in pathogenic bacteria. For this reason, the plasmids such as pUC 18 which are used in recombinant DNA experiments were designed so that they cannot be exchanged with other bacteria except by special t ...
Registration of facility
... Access, Expression and Damage, which together with an overall indication of which level of containment should be used. 9.1 Risk of Access Assess the probability that a microorganism, or the DNA contained within it, will be able to enter the human body and survive there, or survive in the environment ...
... Access, Expression and Damage, which together with an overall indication of which level of containment should be used. 9.1 Risk of Access Assess the probability that a microorganism, or the DNA contained within it, will be able to enter the human body and survive there, or survive in the environment ...
- Bergen.org
... antisense model didn’t make sense: • The antisense technology was used in worms • Puzzling results were produced: both sense and antisense RNA preparations were sufficient to cause interference. • What could be going on? ...
... antisense model didn’t make sense: • The antisense technology was used in worms • Puzzling results were produced: both sense and antisense RNA preparations were sufficient to cause interference. • What could be going on? ...
Chapter 2 Human Genetics Overview The purpose of this chapter is
... The number of chromosomes varies by species. Humans have 46 (23 pairs) while chimpanzees and gorillas have 48 (24 pairs) o About 10 million years ago human ancestral DNA fused two chromosomes together into our modern chromosome 2. Karyotype of Human Chromosomes Not pictured Molecular Genetics 4 ...
... The number of chromosomes varies by species. Humans have 46 (23 pairs) while chimpanzees and gorillas have 48 (24 pairs) o About 10 million years ago human ancestral DNA fused two chromosomes together into our modern chromosome 2. Karyotype of Human Chromosomes Not pictured Molecular Genetics 4 ...
Lecture 10: Nucleic acids (DNA & RNA)
... 1) Deoxyribonucleic acid (DNA): is the genetic material المادة الوراثيةin most organisms (humans, animals, bacteria, plants, and some viruses). 2) Ribonucleic acid (RNA): in some viruses, RNA serves as the genetic material. Nucleic acids store and transmit genetic information المعلومات الوراثي ...
... 1) Deoxyribonucleic acid (DNA): is the genetic material المادة الوراثيةin most organisms (humans, animals, bacteria, plants, and some viruses). 2) Ribonucleic acid (RNA): in some viruses, RNA serves as the genetic material. Nucleic acids store and transmit genetic information المعلومات الوراثي ...
Quantitative analysis to assess the performance of the
... aberrations have previously been detected using optical imaging of whole chromosomes, a technique with limited sensitivity, resolution, quantification, and throughput. Efforts in recent years to use microarrays to overcome these limitations have been hampered by inadequate sensitivity, specificity a ...
... aberrations have previously been detected using optical imaging of whole chromosomes, a technique with limited sensitivity, resolution, quantification, and throughput. Efforts in recent years to use microarrays to overcome these limitations have been hampered by inadequate sensitivity, specificity a ...
Biology
... Livestock given genes for extra growth hormone Poultry given genes to resist infection ...
... Livestock given genes for extra growth hormone Poultry given genes to resist infection ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.