slides - Yin Lab @ NIU
... running (Windows) or a shell terminal is running (Ubuntu) … In any case, you have to close the terminal session (or have it be automatically terminated by the server). If this happens, your program will be terminated without finishing. If you expect your program will run for a very long time, e.g. l ...
... running (Windows) or a shell terminal is running (Ubuntu) … In any case, you have to close the terminal session (or have it be automatically terminated by the server). If this happens, your program will be terminated without finishing. If you expect your program will run for a very long time, e.g. l ...
predominant expression of at cell receptor v,6 gene subfamily
... T Cell Clones . NH2-terminal MBP-specific T cell clones were isolated as described previously, using intact MBP and MBP peptides (2, 3, 5). These clones were isolated from homozygous PL/J mice, except clones F I-12 and F I-21, which were isolated from a (PLSJ)F I mouse (3) . All clones described sha ...
... T Cell Clones . NH2-terminal MBP-specific T cell clones were isolated as described previously, using intact MBP and MBP peptides (2, 3, 5). These clones were isolated from homozygous PL/J mice, except clones F I-12 and F I-21, which were isolated from a (PLSJ)F I mouse (3) . All clones described sha ...
![Great Discoveries in Science: The Double Helix [JUDSON:] In the](http://s1.studyres.com/store/data/002738145_1-0c9d37952d304a3f9a33f852da322160-300x300.png)
Great Discoveries in Science: The Double Helix [JUDSON:] In the
... dismiss DNA was so entrenched that it persisted even after Oswald Avery showed that it can carry genetic information. [CARROLL:] Avery had isolated a substance that conveyed a trait from one bacterium to another. And this "transforming principle," as he called it, he showed that it was not destroyed ...
... dismiss DNA was so entrenched that it persisted even after Oswald Avery showed that it can carry genetic information. [CARROLL:] Avery had isolated a substance that conveyed a trait from one bacterium to another. And this "transforming principle," as he called it, he showed that it was not destroyed ...
Restriction Enzyme digestion of DNA
... • A special class of endonucleases from a bacteria has been isolated for this experiment. These special enzymes, termed restriction endonucleases (RE), digest DNA by breaking bonds only within a specific short sequence of bases. These base sequences usually ran in size from 48 base pairs but can be ...
... • A special class of endonucleases from a bacteria has been isolated for this experiment. These special enzymes, termed restriction endonucleases (RE), digest DNA by breaking bonds only within a specific short sequence of bases. These base sequences usually ran in size from 48 base pairs but can be ...
Construction and Characterization of a Highly Regulable Expression
... Minimal M9 and rich LB media used in this study were prepared as described by Miller (1972). The antibiotics ampicillin, kanamycin, streptomycin, and tetracycline were used in rich media at final concentrations of 100, 40, 200, and 20 g/ml, respectively. When used in minimal media, tetracycline was ...
... Minimal M9 and rich LB media used in this study were prepared as described by Miller (1972). The antibiotics ampicillin, kanamycin, streptomycin, and tetracycline were used in rich media at final concentrations of 100, 40, 200, and 20 g/ml, respectively. When used in minimal media, tetracycline was ...
hered master 4..hered 285 .. Page78
... Ghesquière, 1994) that each gamete in the pentaploid hybrid contains a complete Lm genome. The low frequency (2 per cent) of plants with no Lm PGI/2 alleles shows that a complete Lm genome is nearly always required to provide gamete viability. On the other hand, the existence of a large proportion ...
... Ghesquière, 1994) that each gamete in the pentaploid hybrid contains a complete Lm genome. The low frequency (2 per cent) of plants with no Lm PGI/2 alleles shows that a complete Lm genome is nearly always required to provide gamete viability. On the other hand, the existence of a large proportion ...
... The disaccharide of glucose and N-acetylglucose (shown to the right) can be an effective inhibitor against infection by the virus. As with many other viruses, there is a high rate of mutation in the viral proteins and enzymes. One such mutant enzyme was isolated and the Gln was found to be replaced ...
genetic mapping and dna sequencing of the loblolly pine genome
... We are constructing genetic maps and sequencing genes in loblolly pine (Pinus taeda L.) to gain a deeper understanding of the organization and evolution of pine genomes. Two genetic maps were constructed using restriction fragment length polymorphism (RFLP) markers using complementary DNA (cDNA) pro ...
... We are constructing genetic maps and sequencing genes in loblolly pine (Pinus taeda L.) to gain a deeper understanding of the organization and evolution of pine genomes. Two genetic maps were constructed using restriction fragment length polymorphism (RFLP) markers using complementary DNA (cDNA) pro ...
S13Set #1
... flowers. Pollen from the double plant is used to fertilize several different wild-type plants obtained from the same location in BC. In each case, a 1:1 ratio of single to double is observed in the progeny of each cross. Carefully interpret these data. [Keep in mind that the wild-type parents are ta ...
... flowers. Pollen from the double plant is used to fertilize several different wild-type plants obtained from the same location in BC. In each case, a 1:1 ratio of single to double is observed in the progeny of each cross. Carefully interpret these data. [Keep in mind that the wild-type parents are ta ...
Analysis of mRNA - quantitation (contd)
... inserted DNA » e.g., E. coli H0157 has 800 kb more than lab strains of E. coli, much of which is virulence factors, prophages and prophage like elements – What does this suggest about nature of virulence? Virulence is acquired, i.e, transferred from one organism to another BioSci D145 lecture 5 ...
... inserted DNA » e.g., E. coli H0157 has 800 kb more than lab strains of E. coli, much of which is virulence factors, prophages and prophage like elements – What does this suggest about nature of virulence? Virulence is acquired, i.e, transferred from one organism to another BioSci D145 lecture 5 ...
DNA
... protein? 2. Translation uses the mRNA to build a protein a. In the cytoplasm of the cell, translation occurs at the ribosome. Ribosomes are made of rRNA (ribosomal RNA) and proteins. b. The mRNA “start” codon attaches to the ribosome. The ribosome holds mRNA in place and helps link amino acids toget ...
... protein? 2. Translation uses the mRNA to build a protein a. In the cytoplasm of the cell, translation occurs at the ribosome. Ribosomes are made of rRNA (ribosomal RNA) and proteins. b. The mRNA “start” codon attaches to the ribosome. The ribosome holds mRNA in place and helps link amino acids toget ...
Genetic recombination and mutations - formatted
... The answer to the question as to “How does the genetic variation arise?” lies in understanding the phenomena of mutation. Mutation can be defined as the occurrence of any change in the sequence of nucleic acid or any change in the chromosomal structure. Mutations can also be defined as heritable cha ...
... The answer to the question as to “How does the genetic variation arise?” lies in understanding the phenomena of mutation. Mutation can be defined as the occurrence of any change in the sequence of nucleic acid or any change in the chromosomal structure. Mutations can also be defined as heritable cha ...
Bacteria Transformation
... Objective: Understand How Humans Benefit from Bacterial Transformation New Words: Insulin, recombinant DNA, plasmid, gene splicing The first successful insulin preparations came from cows (and later pigs). In the 1980's technology had advanced to the point where we could make human insulin. The tec ...
... Objective: Understand How Humans Benefit from Bacterial Transformation New Words: Insulin, recombinant DNA, plasmid, gene splicing The first successful insulin preparations came from cows (and later pigs). In the 1980's technology had advanced to the point where we could make human insulin. The tec ...
Genome organization of Magnaporthe grisea
... genetics and molecular biology of this fungus. Three different genetic maps for this organism have been reported (Romao and Hamer 1992; Skinner et al. 1993; Sweigard et al. 1993). One map, containing 98 RFLP markers, two isoenzymes and the mating-type locus (Skinner et al. 1990; Budde et al. 1993; S ...
... genetics and molecular biology of this fungus. Three different genetic maps for this organism have been reported (Romao and Hamer 1992; Skinner et al. 1993; Sweigard et al. 1993). One map, containing 98 RFLP markers, two isoenzymes and the mating-type locus (Skinner et al. 1990; Budde et al. 1993; S ...
The Chicken Genetic Map and Beyond Hans H. Cheng USDA
... unique 6 base-pair sequence. Differences in the DNA sequences between individuals result in differences in DNA fragment lengths after enzyme treatment. These differences are detected by separating the DNA fragments by size on an agarose gel and visualization of the appropriate fragment by Southern b ...
... unique 6 base-pair sequence. Differences in the DNA sequences between individuals result in differences in DNA fragment lengths after enzyme treatment. These differences are detected by separating the DNA fragments by size on an agarose gel and visualization of the appropriate fragment by Southern b ...
Fastest, Easiest Adenoviral System Ever
... cloning procedures to overcome the difficulties with cloning into large (~34 kb) plasmids. Procedures have included precloning into shuttle vectors and subcloning through multiple steps and multiple different strains of E.coli, all of which increase hands-on time and gives more room for error. At Cl ...
... cloning procedures to overcome the difficulties with cloning into large (~34 kb) plasmids. Procedures have included precloning into shuttle vectors and subcloning through multiple steps and multiple different strains of E.coli, all of which increase hands-on time and gives more room for error. At Cl ...
Gene sequencing Terms
... • The term "wild type" allele is sometimes used to describe an allele that is thought to contribute to the typical phenotypic character as seen in "wild" populations of organisms. • Such a "wild type" allele was historically regarded as dominant, common, and "normal", in contrast to "mutant" alleles ...
... • The term "wild type" allele is sometimes used to describe an allele that is thought to contribute to the typical phenotypic character as seen in "wild" populations of organisms. • Such a "wild type" allele was historically regarded as dominant, common, and "normal", in contrast to "mutant" alleles ...
DNA Extraction - Utah Agriculture in the Classroom
... soap and alcohol). This is because you’re not likely starting the protocol with the required amount—1/2 cup—of human cells! That means that you will not extract an amount of DNA large enough to visualize with the naked eye. If you wanted to see it, you would need a centrifuge to spin down (to the bo ...
... soap and alcohol). This is because you’re not likely starting the protocol with the required amount—1/2 cup—of human cells! That means that you will not extract an amount of DNA large enough to visualize with the naked eye. If you wanted to see it, you would need a centrifuge to spin down (to the bo ...
Introducing:
... gene map. It is called a map because it shows where the genes are located down the chromosome. Genes have numbers and letters that make up their names. •You can see how any rearrangement mutations in the chromosomes can alter the order and/or function of gene. •Numerical mutations will affect the nu ...
... gene map. It is called a map because it shows where the genes are located down the chromosome. Genes have numbers and letters that make up their names. •You can see how any rearrangement mutations in the chromosomes can alter the order and/or function of gene. •Numerical mutations will affect the nu ...
DNA Copy Number Analysis (SGF talk 2007-02-12)
... resolution by hybridizing to arrayed genomic DNA or cDNA clones. …However, currently available array CGH methods cannot simultaneously detect chromosomal loss of heterozygosity (LOH). “ SNP Array “… to combine the detection of cancer copy number with cancer-specific LOH in the same experiments, we ...
... resolution by hybridizing to arrayed genomic DNA or cDNA clones. …However, currently available array CGH methods cannot simultaneously detect chromosomal loss of heterozygosity (LOH). “ SNP Array “… to combine the detection of cancer copy number with cancer-specific LOH in the same experiments, we ...
DNA
... EXACTLY the same? A. Sugar phosphate backbone is created the same B. Phosphate bases are dissolve ...
... EXACTLY the same? A. Sugar phosphate backbone is created the same B. Phosphate bases are dissolve ...
FOXP2 and Speech
... FOXP2 protein, which one of the following statements makes the most sense? A. If its FoxP2 gene is removed, the mouse might “talk.” B. Feeding mice the three amino acids that differ between human and mouse might enable the mice to “talk.” C. Replacing the mutated human FOXP2 gene with a mouse FoxP2 ...
... FOXP2 protein, which one of the following statements makes the most sense? A. If its FoxP2 gene is removed, the mouse might “talk.” B. Feeding mice the three amino acids that differ between human and mouse might enable the mice to “talk.” C. Replacing the mutated human FOXP2 gene with a mouse FoxP2 ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.