Resources for the map-based cloning of tga1
... (Genbank AY883436-AY883558) using the PCR primers and conditions listed above. PCR products from Z. diploperennis were cloned into the TA vector (pCR 2.1-TOPO kit, Invitrogen) and at least four clones were sequenced. Nucleotide diversity (π), and Tajima's D statistic (Tajima, 1989, Genetics 123:585- ...
... (Genbank AY883436-AY883558) using the PCR primers and conditions listed above. PCR products from Z. diploperennis were cloned into the TA vector (pCR 2.1-TOPO kit, Invitrogen) and at least four clones were sequenced. Nucleotide diversity (π), and Tajima's D statistic (Tajima, 1989, Genetics 123:585- ...
2007GenomeInformaticsGMODPoster
... genome-scale biological databases. You can use it to create a small laboratory database of genome annotations, or a large webaccessible community database. GMOD includes a modular database schema called Chado that supports many common needs. ...
... genome-scale biological databases. You can use it to create a small laboratory database of genome annotations, or a large webaccessible community database. GMOD includes a modular database schema called Chado that supports many common needs. ...
Slide 1
... 13.2 DNA Technology To make a large quantity of recombinant plasmid DNA, bacterial cells are mixed with recombinant plasmid DNA. Some of the bacterial cells take up the recombinant plasmid DNA through a process called transformation. ...
... 13.2 DNA Technology To make a large quantity of recombinant plasmid DNA, bacterial cells are mixed with recombinant plasmid DNA. Some of the bacterial cells take up the recombinant plasmid DNA through a process called transformation. ...
Biosynthesis of the Antibiotic Nonribosomal Peptide Penicillin in
... bias for incorporation was seen during either of the DNA assembly steps compared to the distribution expected by chance (Fig 2c), whereas a clear bias was seen for strong promoters being required for pclA in all yeast strains able to perform benzylpenicillin biosynthesis (Fig ...
... bias for incorporation was seen during either of the DNA assembly steps compared to the distribution expected by chance (Fig 2c), whereas a clear bias was seen for strong promoters being required for pclA in all yeast strains able to perform benzylpenicillin biosynthesis (Fig ...
Integrons: natural tools for bacterial genome evolution
... exerted by antibiotic use may have driven the specific capture of resistance cassettes from the many different kinds of SI cassette pools through multiple lateral transfers. ...
... exerted by antibiotic use may have driven the specific capture of resistance cassettes from the many different kinds of SI cassette pools through multiple lateral transfers. ...
Real Time PCR Testing for Biotech Crops: Issues
... events are results of single insertion in a given region of genome, thereby resulting in a unique signature site for each transgenes. Results are not influence by copy number of promoter/terminator sequence or copy of transgenes. ...
... events are results of single insertion in a given region of genome, thereby resulting in a unique signature site for each transgenes. Results are not influence by copy number of promoter/terminator sequence or copy of transgenes. ...
Independent specialization of the human and mouse X
... contents of the human and mouse X chromosomes. We first identified all ampliconic regions of the human X chromosome, including those absent from the current reference sequence. We found 24 ampliconic regions present in the reference sequence by searching for duplicated segments of >10 kb in length a ...
... contents of the human and mouse X chromosomes. We first identified all ampliconic regions of the human X chromosome, including those absent from the current reference sequence. We found 24 ampliconic regions present in the reference sequence by searching for duplicated segments of >10 kb in length a ...
ppt - Chair of Computational Biology
... unstructured histone tails through acetylation, methylation and phosphorylation. DNA methylation occurs at 5-position of cytosine residues within CpG pairs in a reaction catalyzed by DNA methyltransferases (DNMTs). ...
... unstructured histone tails through acetylation, methylation and phosphorylation. DNA methylation occurs at 5-position of cytosine residues within CpG pairs in a reaction catalyzed by DNA methyltransferases (DNMTs). ...
18.11
... of map distance can be circumvented by the use of map function (in any organism), and by the Perkin formula (in tetrad-producing organisms such as fungi) ...
... of map distance can be circumvented by the use of map function (in any organism), and by the Perkin formula (in tetrad-producing organisms such as fungi) ...
Student Guide - the BIOTECH Project
... 1. Label the PCR tube so that you can distinguish the samples in the tube. 2. Add 5 µl primer of each primer to each tube. If necessary, gently tap you tube on the counter to get all of the liquid to the bottom of the tube. 3. Add 10 µl GoTaq (green solution). Close the tubes and centrifuge briefly ...
... 1. Label the PCR tube so that you can distinguish the samples in the tube. 2. Add 5 µl primer of each primer to each tube. If necessary, gently tap you tube on the counter to get all of the liquid to the bottom of the tube. 3. Add 10 µl GoTaq (green solution). Close the tubes and centrifuge briefly ...
Detection of unpaired DNA at meiosis results in RNA‐mediated
... half.(1) Homozygous Sad-1UV crosses are barren with all asci arrested at meiotic prophase. This phenotype allowed mapping to linkage group I and cloning of sad-1þ by complementation.(2) It also has provided a more efficient method for the isolation of further Sad-1 mutations. A complete sad-1 deleti ...
... half.(1) Homozygous Sad-1UV crosses are barren with all asci arrested at meiotic prophase. This phenotype allowed mapping to linkage group I and cloning of sad-1þ by complementation.(2) It also has provided a more efficient method for the isolation of further Sad-1 mutations. A complete sad-1 deleti ...
The Complete Chloroplast and Mitochondrial DNA Sequence of
... PHYML 2.4.4 (Guindon and Gascuel 2003) was used to compute maximum likelihood trees, using the cpREV45 model for cp sequences and the Hasegawa, Kishino and Yano (1985) model for the combined nucleic acid data set. Pairwise distance trees were obtained using TREECON (Van de Peer and De Wachter 1994), ...
... PHYML 2.4.4 (Guindon and Gascuel 2003) was used to compute maximum likelihood trees, using the cpREV45 model for cp sequences and the Hasegawa, Kishino and Yano (1985) model for the combined nucleic acid data set. Pairwise distance trees were obtained using TREECON (Van de Peer and De Wachter 1994), ...
Human-Genetics-Concepts-and-Applications-9E
... certain single nucleotide polymorphisms (SNPs) are associated with response to one drug but not another. This is an approach called A. gene therapy. B. pharmacogenomics. C. genetic determinism. D. applied pharmacology. E. genotherapy. ...
... certain single nucleotide polymorphisms (SNPs) are associated with response to one drug but not another. This is an approach called A. gene therapy. B. pharmacogenomics. C. genetic determinism. D. applied pharmacology. E. genotherapy. ...
Comparison of Deoxyribonucleic Acid Homologies of Six Strains of
... could be separated from one another based upon differences in polynucleotide sequence homologies and to determine whether there are any polynucleotide sequence homologies among the four genera of ammonia-oxidizing bacteria which we examined. In the past, DNA-DNA hybridization studies involving the a ...
... could be separated from one another based upon differences in polynucleotide sequence homologies and to determine whether there are any polynucleotide sequence homologies among the four genera of ammonia-oxidizing bacteria which we examined. In the past, DNA-DNA hybridization studies involving the a ...
8.2 Structure of DNA
... • An operator is a part of DNA that turns a gene “on” or ”off.” • An operon includes a promoter, an operator, and one or more structural genes that code for all the proteins needed to do a job. – Operons are most common in prokaryotes. – The lac operon was one of the first examples of gene regulatio ...
... • An operator is a part of DNA that turns a gene “on” or ”off.” • An operon includes a promoter, an operator, and one or more structural genes that code for all the proteins needed to do a job. – Operons are most common in prokaryotes. – The lac operon was one of the first examples of gene regulatio ...
Comparison of Deoxyribonucleic Acid Homologies of Six Strains of
... could be separated from one another based upon differences in polynucleotide sequence homologies and to determine whether there are any polynucleotide sequence homologies among the four genera of ammonia-oxidizing bacteria which we examined. In the past, DNA-DNA hybridization studies involving the a ...
... could be separated from one another based upon differences in polynucleotide sequence homologies and to determine whether there are any polynucleotide sequence homologies among the four genera of ammonia-oxidizing bacteria which we examined. In the past, DNA-DNA hybridization studies involving the a ...
Bioreg2017_Replication3_V4
... You have a 100 bp sequence element whose function you can quantitatively assay after introducing it into cells You have a protein that you purified based on its ability to bind the sequence element and you wonder whether this protein is required for the function of the sequence element. Hence, you ...
... You have a 100 bp sequence element whose function you can quantitatively assay after introducing it into cells You have a protein that you purified based on its ability to bind the sequence element and you wonder whether this protein is required for the function of the sequence element. Hence, you ...
Bio 211 Genetics Laboratory Experiment 5: Bioinformatics
... Bioinformatics is the field and study of biological information in DNA using computer‐ based approaches. Through program algorithms, coding sequences, promoters, and other functional DNA sequences can be identified from databases of genomic information, and interspecific comparisons can be made t ...
... Bioinformatics is the field and study of biological information in DNA using computer‐ based approaches. Through program algorithms, coding sequences, promoters, and other functional DNA sequences can be identified from databases of genomic information, and interspecific comparisons can be made t ...
Identification of an Insertion Sequence Located
... insertion in front of a gene affects the expression of a downstream gene(s) (11). IS can also mediate deletions, duplications, and inversions and cointegrate formation contributing to changes in the bacterial genome. Virulence genes in pathogenic bacteria are often located together on the chromosome ...
... insertion in front of a gene affects the expression of a downstream gene(s) (11). IS can also mediate deletions, duplications, and inversions and cointegrate formation contributing to changes in the bacterial genome. Virulence genes in pathogenic bacteria are often located together on the chromosome ...
Genome evolution: a sequence
... The specificity of the TF binding is central to the understanding of the regulatory relations it can form. We are therefore interested in defining the DNA motifs that can be recognize by each TF. A simple representation of the binding motif is the consensus site, usually derived by studying a set of ...
... The specificity of the TF binding is central to the understanding of the regulatory relations it can form. We are therefore interested in defining the DNA motifs that can be recognize by each TF. A simple representation of the binding motif is the consensus site, usually derived by studying a set of ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.