PCR Techniques
... Annealing temp ideally >55C (portion that anneals to your template) Hairpins Tm<50 ? Self dimers---only important if they are 3’ annealing dimers Silent mutants---better to have them on 5’ end than on 3’ end ...
... Annealing temp ideally >55C (portion that anneals to your template) Hairpins Tm<50 ? Self dimers---only important if they are 3’ annealing dimers Silent mutants---better to have them on 5’ end than on 3’ end ...
What`s New
... 2. There is an updated version of GeneMark (2.8) for bacterial models. It can be found at http://exon.gatech.edu/gmhmm2_prok.cgi. This is relatively new and untested. Preliminary data suggests it will evaluate the genomes identically to GeneMark 2.5. 3. When using the web-based GeneMark against a mo ...
... 2. There is an updated version of GeneMark (2.8) for bacterial models. It can be found at http://exon.gatech.edu/gmhmm2_prok.cgi. This is relatively new and untested. Preliminary data suggests it will evaluate the genomes identically to GeneMark 2.5. 3. When using the web-based GeneMark against a mo ...
Touring Ensembl: A practical guide to genome browsing Open Access
... The Ensembl genome browser and underlying databases focus on chordate organisms. More species such as plants and microorganisms can be investigated using our sister browser at http://www.ensemblgenomes.org. In this study, four examples are used that sample many pages and features of the Ensembl brow ...
... The Ensembl genome browser and underlying databases focus on chordate organisms. More species such as plants and microorganisms can be investigated using our sister browser at http://www.ensemblgenomes.org. In this study, four examples are used that sample many pages and features of the Ensembl brow ...
The epigenetic basis of gender in flowering plants and mammals
... Melissa Spielman, Rinke Vinkenoog, Hugh G. Dickinson and Rod J. Scott What makes a sperm male or an egg female, and how can we tell? A gamete’s gender could be defined in many ways, such as the sex of the individual or organ that produced it, its cellular morphology, or its behaviour at fertilizatio ...
... Melissa Spielman, Rinke Vinkenoog, Hugh G. Dickinson and Rod J. Scott What makes a sperm male or an egg female, and how can we tell? A gamete’s gender could be defined in many ways, such as the sex of the individual or organ that produced it, its cellular morphology, or its behaviour at fertilizatio ...
A strategy for extracting and analyzing large
... mutation must be considered when evaluating the phenotype of the double mutant. Indeed, a double mutant could have a more severe phenotype than either single mutant and still represent a synthetic, neutral, or alleviating interaction. Typically, large-scale studies have scored gene-gene interactions ...
... mutation must be considered when evaluating the phenotype of the double mutant. Indeed, a double mutant could have a more severe phenotype than either single mutant and still represent a synthetic, neutral, or alleviating interaction. Typically, large-scale studies have scored gene-gene interactions ...
a nucleosomal perspective
... transcription factors on gene regulation: a nucleosomal perspective ...
... transcription factors on gene regulation: a nucleosomal perspective ...
Molecular Plant-Microbe Interactions
... al. 1991). The NodE protein has been localized in the cytoplasmic membrane (Spaink et al. 1989) and is similar to the fatty acid synthases of E. coli (FabB) and Saccharomyces cerevisiae; it is also similar to the putative β-ketoacyl synthase of a Streptomyces sp. (Bibb et al. 1989). Therefore, it is ...
... al. 1991). The NodE protein has been localized in the cytoplasmic membrane (Spaink et al. 1989) and is similar to the fatty acid synthases of E. coli (FabB) and Saccharomyces cerevisiae; it is also similar to the putative β-ketoacyl synthase of a Streptomyces sp. (Bibb et al. 1989). Therefore, it is ...
Click
... mber of probes to the gene RNA. RNA-seq gene expression as the fraction of aligned reads that can be assigned to the before expression e Expression estimator value ...
... mber of probes to the gene RNA. RNA-seq gene expression as the fraction of aligned reads that can be assigned to the before expression e Expression estimator value ...
Revised Tetrahymena Nomenclature Rules
... Example: CU428 is the strain number 428 created at the Cornell University. 2. Strain B (whose genome has been sequenced by The Institute for Genomic Research) serves as the reference strain; all loci from inbred strain B need no further identification. 3. Genes from all other inbred strains and natu ...
... Example: CU428 is the strain number 428 created at the Cornell University. 2. Strain B (whose genome has been sequenced by The Institute for Genomic Research) serves as the reference strain; all loci from inbred strain B need no further identification. 3. Genes from all other inbred strains and natu ...
et al - International Journal of Systematic and Evolutionary
... these proteins was previously available from only a limited number of actinobacteria, whose genomes have been sequenced. One possible signature for actinobacteria, consisting of a large insert in the 23S rRNA, has previously been described (Roller et al., 1992). However, the validity and specificity ...
... these proteins was previously available from only a limited number of actinobacteria, whose genomes have been sequenced. One possible signature for actinobacteria, consisting of a large insert in the 23S rRNA, has previously been described (Roller et al., 1992). However, the validity and specificity ...
Identifying a Novel Isoform of the AZIN1 Gene by Combining High
... reading frame that would change the terminus of the subsequent protein from Ser-Asp-Glu-Asp-stop to PheArg-stop. Follow-up studies could validate this finding on the protein level and then measure gene expression of this new isoform in various tissues, subjects, and time-points. Moreover, the method ...
... reading frame that would change the terminus of the subsequent protein from Ser-Asp-Glu-Asp-stop to PheArg-stop. Follow-up studies could validate this finding on the protein level and then measure gene expression of this new isoform in various tissues, subjects, and time-points. Moreover, the method ...
1995 Broad et al: CURRENT STATE OF THE NEW ZEALAND
... approach appears to have succeeded in localizing quantitative trait loci for growth and famess in pigs (Andersson et al. 1994) to particular chromosomal segments, but more testing of the applicability of this approach for identifying quantitative trait loci is still required. Exploration of such loc ...
... approach appears to have succeeded in localizing quantitative trait loci for growth and famess in pigs (Andersson et al. 1994) to particular chromosomal segments, but more testing of the applicability of this approach for identifying quantitative trait loci is still required. Exploration of such loc ...
Nature Genetics - David Page Lab
... evolved from a pair of ordinary autosomes. At first, sex was genetically determined by a simple diallelic system, F and M, in which the male was the heterogametic sex. b, Sex chromosome differentiation began when the proto-Y chromosome accrued at least one additional gene, that together with the M a ...
... evolved from a pair of ordinary autosomes. At first, sex was genetically determined by a simple diallelic system, F and M, in which the male was the heterogametic sex. b, Sex chromosome differentiation began when the proto-Y chromosome accrued at least one additional gene, that together with the M a ...
Gene targeting in filamentous fungi: the benefits of impaired repair
... et al. 2004). By crossing out the genetic lesion, this potential drawback can be minimized in sexually propagating fungi. For deuteromycetes however, other and more tedious approaches need to be developed to ensure transient inactivation of the Ku complex; this is considered necessary especially for ...
... et al. 2004). By crossing out the genetic lesion, this potential drawback can be minimized in sexually propagating fungi. For deuteromycetes however, other and more tedious approaches need to be developed to ensure transient inactivation of the Ku complex; this is considered necessary especially for ...
Slides
... – Does a motif also appears in the homologous genes of another species? – Strongest evidence – However, will not be able to find species-specific ones ...
... – Does a motif also appears in the homologous genes of another species? – Strongest evidence – However, will not be able to find species-specific ones ...
1548 Tn Gene Is Borne by Composite Transposon Aminoglycoside
... 34 clinical isolates of enterobacteria, collected in several countries, likely to produce a CTX-M enzyme since they were more resistant to cefotaxime than to ceftazidime (Table 1), the blaCTX-M-3 gene was identified in 14 strains, the blaCTX-M-2 gene was identified in 1 strain, the blaCTX-M-9 gene w ...
... 34 clinical isolates of enterobacteria, collected in several countries, likely to produce a CTX-M enzyme since they were more resistant to cefotaxime than to ceftazidime (Table 1), the blaCTX-M-3 gene was identified in 14 strains, the blaCTX-M-2 gene was identified in 1 strain, the blaCTX-M-9 gene w ...
Number 52, 2005 11 Robert L. M etzenberg
... to mus-51. (These exceptions will be dealt with later.) his-3; mus-51 a (FGSC #9538) - (normal sequence) is transformed to hygromycin resistance with the yfg knockout cassette (but obviously will still require histidine, and they must be held under selection with hygromycin). This strain carries mus ...
... to mus-51. (These exceptions will be dealt with later.) his-3; mus-51 a (FGSC #9538) - (normal sequence) is transformed to hygromycin resistance with the yfg knockout cassette (but obviously will still require histidine, and they must be held under selection with hygromycin). This strain carries mus ...
Genetics of quantitative traits and the Central Limit Theorem
... Have you heard of Darwin Finches from the Galapagos Islands and how they are used to illustrate the interaction between evolution and ecology (beak length variation)? Have you heard explanations of evolutionary changes that go all the way to the effect of multiple genes? Well, Ricklefs, my undergrad ...
... Have you heard of Darwin Finches from the Galapagos Islands and how they are used to illustrate the interaction between evolution and ecology (beak length variation)? Have you heard explanations of evolutionary changes that go all the way to the effect of multiple genes? Well, Ricklefs, my undergrad ...