Slajd 1
... Applications of the PCR 1 – Detection of the polymorphisms 2 – Diagnostics of hereditary diseases 3 – Sequencing (detection of mutations, paternity tests) 4 – Detection of viruses, parasites and bacteria 5 – Detection of GMOs 6 – In situ PCR (detection of given sequences in given subcellular localiz ...
... Applications of the PCR 1 – Detection of the polymorphisms 2 – Diagnostics of hereditary diseases 3 – Sequencing (detection of mutations, paternity tests) 4 – Detection of viruses, parasites and bacteria 5 – Detection of GMOs 6 – In situ PCR (detection of given sequences in given subcellular localiz ...
Bioinformatics: Integrative Analyses of Genome
... found that a number of folds, such as TIM-barrels, recur often in every (analyzed) genome, while other folds are missing from certain genomes. Our analyses have also found many global, statistical differences between protein folds from different phylogenetic groups -- e.g. longer and more numerous a ...
... found that a number of folds, such as TIM-barrels, recur often in every (analyzed) genome, while other folds are missing from certain genomes. Our analyses have also found many global, statistical differences between protein folds from different phylogenetic groups -- e.g. longer and more numerous a ...
Supplementary material for Part XY (Siepel lab analysis)
... “European”, “Asian”, “African”, or “unknown” was made. This was done by tracing the two lineages coming from an individual (one for each parent) and determining which other individual either of these lineages shares the most recent ancestry with. No assignment was made in the case of a tie with mult ...
... “European”, “Asian”, “African”, or “unknown” was made. This was done by tracing the two lineages coming from an individual (one for each parent) and determining which other individual either of these lineages shares the most recent ancestry with. No assignment was made in the case of a tie with mult ...
Genetic Engineering Notes
... Genetically Modified Organisms (GMOs) = are organisms with artificially altered DNA. They can be created by: Inserting a foreign gene: Organisms that are altered in this way are known as transgenic organisms. ...
... Genetically Modified Organisms (GMOs) = are organisms with artificially altered DNA. They can be created by: Inserting a foreign gene: Organisms that are altered in this way are known as transgenic organisms. ...
genes: genetics, gemonics, an evolution
... d. a sticky end must be available for the ligase enzyme to function. e. all of these must occur. ____ 23. Gel electrophoresis separates the DNA fragments according to a. their length. b. their mass. c. their speed of travel through the gel. d. the number of nucleotides in the fragment. e. all of the ...
... d. a sticky end must be available for the ligase enzyme to function. e. all of these must occur. ____ 23. Gel electrophoresis separates the DNA fragments according to a. their length. b. their mass. c. their speed of travel through the gel. d. the number of nucleotides in the fragment. e. all of the ...
Downstream analysis of transcriptomic data
... • Fisherʼs exact test is used for ORA of gene lists for a single type of annota3on. • P-‐value for Fisherʼs exact test – is “the probability that a random draw of the same size as the g ...
... • Fisherʼs exact test is used for ORA of gene lists for a single type of annota3on. • P-‐value for Fisherʼs exact test – is “the probability that a random draw of the same size as the g ...
Genes
... There are probably more described species of macroscopic organisms than of microscopic organisms. Surveys of molecular diversity suggests that the majority of biological diversity is in the microorganisms. There is evidence that many microscopic species haven't been discovered, or are known only ...
... There are probably more described species of macroscopic organisms than of microscopic organisms. Surveys of molecular diversity suggests that the majority of biological diversity is in the microorganisms. There is evidence that many microscopic species haven't been discovered, or are known only ...
Ensembl
... ‘One shot’ sequencing of cDNA from the 5’ and 3’ end creates the EST sequence. ESTs are only 500-800 nucleotides long Low quality fragment- sequence error of ~2%. ...
... ‘One shot’ sequencing of cDNA from the 5’ and 3’ end creates the EST sequence. ESTs are only 500-800 nucleotides long Low quality fragment- sequence error of ~2%. ...
Rapid Communication: Mapping of the Titin (TTN) Gene to Pig
... G. R. Bertani2, N. J. Larsen3, S. Marklund, Z. L. Hu, and M. F. Rothschild4 Department of Animal Science, Iowa State University, Ames 50011 ...
... G. R. Bertani2, N. J. Larsen3, S. Marklund, Z. L. Hu, and M. F. Rothschild4 Department of Animal Science, Iowa State University, Ames 50011 ...
A grand challenge for nutrigenomics
... et al., 2010; Parra et al., 2010), but little data are available describing miRNA-level modulation of genes of metabolism. Finally, genetic variation influences eating behaviors (Dotson et al., 2010; Fan et al., 2010) but these effects have not been systematically explored (this is an exciting pot ...
... et al., 2010; Parra et al., 2010), but little data are available describing miRNA-level modulation of genes of metabolism. Finally, genetic variation influences eating behaviors (Dotson et al., 2010; Fan et al., 2010) but these effects have not been systematically explored (this is an exciting pot ...
Recombinant DNA
... • Bacteria lack introns. • Lack ability to remove introns from RNA transcribed by eukaryotic genes. • Use retroviruses – they have reverse transcriptase. • Copy a messenger RNA to make an intron-less DNA called cDNA. • Then put that DNA into a bacterial host. • Expression of a eukaryotic gene. ...
... • Bacteria lack introns. • Lack ability to remove introns from RNA transcribed by eukaryotic genes. • Use retroviruses – they have reverse transcriptase. • Copy a messenger RNA to make an intron-less DNA called cDNA. • Then put that DNA into a bacterial host. • Expression of a eukaryotic gene. ...
Agaba et al - Centre for Genomic Research
... only 51 genes (shown in the table below. Note that these results are based on an analysis with EnsEMBL bovine genome preliminary build Btau2.0. A more recent preliminary build is available, and the analysis will be repeated, and key findings discussed in a future publication). ...
... only 51 genes (shown in the table below. Note that these results are based on an analysis with EnsEMBL bovine genome preliminary build Btau2.0. A more recent preliminary build is available, and the analysis will be repeated, and key findings discussed in a future publication). ...
word - Mr Idea Hamster
... sequence. These will be the same for everyone and the instructor will use these values in the other four parts of the course. 2. An individual chromosome, disease, gene, protein, nucleotide sequence, and amino acid sequence. These will be different for everyone (every pair?). The student will use he ...
... sequence. These will be the same for everyone and the instructor will use these values in the other four parts of the course. 2. An individual chromosome, disease, gene, protein, nucleotide sequence, and amino acid sequence. These will be different for everyone (every pair?). The student will use he ...
Systematics powerpoint - Westgate Mennonite Collegiate
... Remember, trees such as these do not say that humans descend from other modern primates. “Man came from monkeys” is a common misperception of what evolution means. Phylogenetic trees trace common shared genes between groups, and infer shared ancestors based on relationships between modern organisms ...
... Remember, trees such as these do not say that humans descend from other modern primates. “Man came from monkeys” is a common misperception of what evolution means. Phylogenetic trees trace common shared genes between groups, and infer shared ancestors based on relationships between modern organisms ...
cover letter - Annals of Gastroenterology
... The current study is an interesting investigation in public databases with microarrays data regarding the genetic profile of the most common GI cancer. Despite the high numbers of samples and genes analyzed the studies presented several weaknesses which should be addressed before the manuscript is c ...
... The current study is an interesting investigation in public databases with microarrays data regarding the genetic profile of the most common GI cancer. Despite the high numbers of samples and genes analyzed the studies presented several weaknesses which should be addressed before the manuscript is c ...
Chapter 4 Sequencing DNA and Databases
... Scientists therefore wanted to examine the sequences of the DNA they were working with. The first DNA sequences were determined by very laborious methods that generated relative short sequences. Rapid DNA sequencing methods were developed in the mid 1970's which allowed scientists to generate more s ...
... Scientists therefore wanted to examine the sequences of the DNA they were working with. The first DNA sequences were determined by very laborious methods that generated relative short sequences. Rapid DNA sequencing methods were developed in the mid 1970's which allowed scientists to generate more s ...
... - Copy numbers gains >2Mb and losses >1Mb, including at least one OMIM annotated gene are reported in this analysis. - Gains/losses of >50 Kb within custom clinically significant gene set. On request candidate genes can be analyzed at a much lower threshold, depending on gene specific marker density ...
... chromosome of >20 Mb interstitially or >10 Mb telomerically (15 and 8 Mb, respectively, for imprinted chromosomes). * Contiguous homozygosity of >8 Mb within multiple chromosomes suggests common descent. These regions of potential recessive allele risk are designated. * A high level of allele homozy ...
3000_2013_1e
... • remember: homoplasy may be evolutionary convergence/reversal, may also reflect our own uncertainty about the character and how it develops! ...
... • remember: homoplasy may be evolutionary convergence/reversal, may also reflect our own uncertainty about the character and how it develops! ...
Using Blast To Ask Questions About Evolutionary Relationships
... Evolutionary Relationships One of the tools used to determine how recently two species share a common organism is to compare their molecular sequences. Species that share a relatively recent common ancestor will have fewer differences than species that diverged in the more distant past. By comparing ...
... Evolutionary Relationships One of the tools used to determine how recently two species share a common organism is to compare their molecular sequences. Species that share a relatively recent common ancestor will have fewer differences than species that diverged in the more distant past. By comparing ...
Document
... Knowledge of which genes in an organism are essential and under what conditions they are essential is of fundamental and practical importance. This knowledge provides us with a unique tool to refine the interpretation of cellular networks and to map critical points in these networks. From a modelin ...
... Knowledge of which genes in an organism are essential and under what conditions they are essential is of fundamental and practical importance. This knowledge provides us with a unique tool to refine the interpretation of cellular networks and to map critical points in these networks. From a modelin ...
Prokaryotic genome-size evolution Range of C values in prokaryotes
... function exclusively in the mitochondria. Presumably, some of these genes were once part of the mitochondrial genome which currently contains 28 proteincoding genes. ...
... function exclusively in the mitochondria. Presumably, some of these genes were once part of the mitochondrial genome which currently contains 28 proteincoding genes. ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.