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Human Genome and Human Genome Project
Human Genome and Human Genome Project

... • Each chromosome contains many genes, the basic physical and functional units of heredity. Genes are specific sequences of bases that encode instructions on how to make proteins. • Proteins perform most life functions and even make up the majority of cellular structures. Proteins are large, complex ...
Lecture#7 - Eukaryote gene structure and regulation.
Lecture#7 - Eukaryote gene structure and regulation.

... Griffiths (2008) 9th Ed. Readings: pp 33-36, 385-402; 300-312 Problems: 9th Ed. Ch. 8: 2,3,6,8,12-14,18 Ch 11: 1,2,5,16 Campbell (2008) 9th Ed. Readings: Concept 17.1-3, 18.2 ...
Microbial Source Tracking • Pathogen Contamination  –What is it?
Microbial Source Tracking • Pathogen Contamination –What is it?

... qPCR in Microbial Source Tracking • 16S rRNA gene found in nearly all bacteria and Archaea • Small changes in genes allow for identification of hosts • qPCR allows for quantification of specific host inputs ...
The Importance of Marine Genomics to Life
The Importance of Marine Genomics to Life

... simultaneous study of many components and their interactions with the environment from pathways, through cell tissues to whole organisms and communities (Hollywood et al., 2006; Joyce and Palsson, 2006). The importance of this field has been supported by the concurrent development of many new techno ...
From Restriction Maps to Cladograms
From Restriction Maps to Cladograms

... genes which code for hemoglobin. 1.Compare the restriction map for each species to the human map. Make a mark on the map for each difference. A difference is the addition or subtraction of a restriction site. (See the example). 2.For each restriction map, count the number of differences that you fin ...
Comparative Gene Expression Analysis: Data Analysis Issues
Comparative Gene Expression Analysis: Data Analysis Issues

... • Goal: gain biological insights by analyzing which genes have the same or divergent behavior across the two organisms • Techniques can identify pairs of orthologous genes between two organisms – C. albicans and S cerevisiae have 4000 such pairs ...
Practice Exam 2 - Montgomery College
Practice Exam 2 - Montgomery College

... A) Produced by fungi. B) Produced by bacteria. C) Synthesized in laboratories. D) Produced by Fleming. E) Produced by eukaryotic organisms. 16) Which of the following antibiotics does NOT interfere with cell wall synthesis? A) Cephalosporins B) Macrolides C) Natural penicillins D) Semisynthetic peni ...
modules_tutorial
modules_tutorial

... As an information resource, Gramene's purpose is to provide added value to data sets available within the public sector to facilitate researchers' ability to leverage the rice genomic sequence to identify and understand corresponding genes, pathways and phenotypes in the crop grasses. This is achiev ...
Catalogue of Expressed Sequence Tags (ESTs) from
Catalogue of Expressed Sequence Tags (ESTs) from

... Glycyphagus domesticus had an additional 8 allergens distinct to itself. These were dust mite allergen groups 5 and7, tropomyosin and superoxide dismutase. It also contained allergens from other organisms such as plants (Juniperus virginiana and Hevea brasiliensis), yeast (Malassezia sympodialis), a ...
12_Clicker_Questions
12_Clicker_Questions

... c. the second to the bottom fragment d. the bottom fragment (the smallest) © 2010 Pearson Education, Inc. ...
Foundations in Microbiology
Foundations in Microbiology

... • Plasmids – small, well characterized, easy to manipulate and can be transferred into appropriate host cells through transformation • Bacteriophages – have the natural ability to inject their DNA into bacterial hosts through transduction ...
Foundations in Microbiology
Foundations in Microbiology

... • Plasmids – small, well characterized, easy to manipulate and can be transferred into appropriate host cells through transformation • Bacteriophages – have the natural ability to inject their DNA into bacterial hosts through transduction ...
The Polymerase Chain Reaction (PCR) provides an extremely
The Polymerase Chain Reaction (PCR) provides an extremely

... G at positions 1, 12, ... and C at positions 6, 7, 8, 10, 15... and T at positions 4, 5, 9, 14.... then we can reconstruct the sequence Obtaining this information is conceptually quite simple. The idea is to cause a termination of a growing DNA chain at a known base (A,G,C or T) and at a known locat ...
The Two Versions of the Human Genome - Max-Planck
The Two Versions of the Human Genome - Max-Planck

... with a length of 40,000 base pairs are inserted into bacterial transport DNA and these so-called fosmids (representing now haploid DNA fragments) are reproduced in bacteria. Approximately 1.44 million fosmids are distributed on three plates, each containing 96 wells, representing the fosmid library ...
Infectious Disease Review
Infectious Disease Review

... In patient 1 a single infection may lead to two clonal types due to evolution of the microbe. These clones may be highly similar and vary by as little as a single nucleotide mutation anywhere in the genome. In patient 2 there are several unrelated differences between the clones. This may be due to m ...
go-interpretation-analysis-2014
go-interpretation-analysis-2014

... GO has made a term enrichment tool available on the website  Beginning to use our own data in the same way our users most commonly use it  This was not a goal of the GO grant. Instead, we had proposed:  We will define test datasets that will allow software developers to benchmark their products. ...
ZNF232: structure and expression analysis of a novel human C2H2
ZNF232: structure and expression analysis of a novel human C2H2

... respectively) representing the entire ZNF232 translated region; the ampli¢ed DNA was inserted into the bluntended HindIII site of the vector polylinker. A deletion derivative, pEGFP/delZNF232, lacking the NLS region was prepared by excision of a KpnI fragment de¢ned by the unique internal (nt positi ...
File
File

... 1. Archaea – discovered in the 1970’s, these are the “ancient” bacteria. They are also called “extremists” because they survive in extreme conditions. No archaea are human pathogens. They are so different from normal bacteria that they have their own group. They thrive in conditions that mimic those ...
Chapter 1
Chapter 1

... • Most common type in human infection • Members widely diverse • Most prominent features include: – Specific shapes • Rod-shaped, spherical and spiral ...
Applications Lecture 4 - Rose
Applications Lecture 4 - Rose

... individual proteins. (the production of these proteins is known as gene expression) b. Gene expression takes place in two stages i. Transcription—DNA is turned into RNA via the enzyme RNA polymerase. ii. Translation—RNA is turned into Protein in the rough Endoplasmic Reticulum found in the cytoplasm ...
Classification (Supervised Clustering)
Classification (Supervised Clustering)

... 2. Cluster the genes and take the most significantly differentially expressing gene in each cluster. 3. Add variables to your discrimination function stepwise. 4. PAM - shrink the group center to the overall center, and then apply a robust QDA with moderated variance estimates (like SAM). The method ...
REMTEC 29sep - site characterisation
REMTEC 29sep - site characterisation

... and bacterial distribution in groundwater and matrix. Understanding of all the complex processes and where these takes place. Investigate which microorganisms that are present, as well as their ability to move from permeable layers into the clayey till matrix. ...
stranded DNA from genomic library
stranded DNA from genomic library

... • STR – Short sequences of DNA repeated many times in a row – STR analysis compared lengths of STR sequences at specific sites on the genome ...
Insert Presentation title here
Insert Presentation title here

... Metabolic Syndrome Does Metabolic Syndrome exist or is it a sum of its risk components? ...
Slajd 1
Slajd 1

... Applications of the PCR 1 – Detection of the polymorphisms 2 – Diagnostics of hereditary diseases 3 – Sequencing (detection of mutations, paternity tests) 4 – Detection of viruses, parasites and bacteria 5 – Detection of GMOs 6 – In situ PCR (detection of given sequences in given subcellular localiz ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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