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Environmental Pollution
Environmental Pollution

... above-mentioned target genes. This method provided a clone library, and sequencing revealed information on new prokaryotes which grouped mainly next to uncultured microorganisms. However, due to the high diversity of approximately 104 ribotypes (wdifferent bacteria) in soils (Torsvik et al., 1990), ...
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... - What mRNAs are present in the cell and in what quantities => inferences regarding the state of the cell. - Transcriptome: The complete collection of the organism’s mRNAs . - Why not study the proteins? - The function of a protein is determined not just by its amino acid sequence, but also the spec ...
Chapter Two Line Title Here and Chapter Title Here and Here
Chapter Two Line Title Here and Chapter Title Here and Here

... Heat is one of the older and more common means of microbial control. High temperatures denature proteins, interfere with the integrity of cytoplasmic membranes and cell walls, and disrupt the function and structure of nucleic acids. Microorganisms vary in their susceptibility to heat. The thermal de ...
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... Begun in 1990, the U.S. Human Genome Project is a 13-year effort coordinated by the U.S. Department of Energy and the National Institutes of Health. The project originally was planned to last 15 years, but effective resource and technological advances have accelerated the expected completion date to ...
Comparative Sequence Analysis between Human and Mouse
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... We aligned promoter sequences of human and mouse. The distribution of alignment scores had two peaks; a major peak around 1000, and a minor peak lower than 100. When we aligned non-orthologous promoters generated by shuffling pairs, the score distribution precisely fit the minor peak of orthologous ...
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... Cloning DNA means to isolate a gene or fragment of DNA away from the other DNA of an organism and be able to propagate this piece. 2. What is a vector? Vectors (or plasmids) are naturally occurring small pieces of circular DNA that can replicate in various organisms. In this course we concentrate on ...
Human Genome Project - the Centre for Applied Genomics
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... the human genome. Based on the size of the human genome and the results from the Drosophila experiment, it was predicted that over 70 million sequencing reactions would need to be completed. The alignment of the resulting sequence assemblies along the genome would be accomplished using the large num ...
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... general, involves four main steps. First, a metagenomic library containing a pool of biocatalyst-encoding genes is constructed from a marine environment, which can be done by various methods, including cloning of enzymatically-digested DNA, uncut DNA, and PCR-amplified products. Second, the metageno ...
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BIOLOGY - Learner
BIOLOGY - Learner

... By selecting a particular class of morphological characters, researchers may also bias the analysis in such a way that groups with certain characteristics cluster with others for reasons other than homology. For instance, if the set of characters were weighted toward those involved in carnivory, car ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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