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Analyzing Copy Number Variation in the Human Genome
Analyzing Copy Number Variation in the Human Genome

... ** - 39 healthy controls, 16 with karyotype abnormalities *** - accounting for only those sites that showed in 2 or more individuals ...
Microsatellite Repeat Variation Within the y1 Gene of Maize and
Microsatellite Repeat Variation Within the y1 Gene of Maize and

... copies of the pentanucleotide repeat but differ by a single base in the first repeat. We have further subdivided these categories based on the number of (CCA)n repeats found. The only sequence variability found within the (CCA)n repeat was a C to T transition in the second and fifth (CCA)n repeats o ...
An Introduction to Bioconductor_2013
An Introduction to Bioconductor_2013

... Now we are familiar with the data, we could go the next step and do the analysis... ...
The GMOD Project: Creating Reusable Software Components
The GMOD Project: Creating Reusable Software Components

...  Chado is very good at capturing complex biological data, but…  It is a data warehouse, and so can be a little slow to query, so…  If you have only features on sequences, you probably want something else (but I’ve got that too) ...
Text S1.
Text S1.

... Criteria to define trans-specifically shared S-alleles Based on the mismatch distribution for interspecific pairs of SRK sequences between A. lyrata and A. halleri (Fig. 2), we defined "trans-specifically shared S-alleles", i.e. alleles assumed to have evolved from a single S-allele in the direct an ...
An Introduction to Bioconductor_2014
An Introduction to Bioconductor_2014

... Now we are familiar with the data, we could go the next step and do the analysis... ...
CHAPTER 20
CHAPTER 20

... Alternatively, the goal may be to prepare many copies of the gene itself. ...
Location and Characterization of the Bovine Herpesvirus Type 2
Location and Characterization of the Bovine Herpesvirus Type 2

... Apart from the disease caused by BHV-2 this virus is also of interest as it is one of the few nonhuman herpesviruses whose D N A bears nucleotide sequence homology with the HSV-1 genome (Sterz et al., 1973/74). A direct comparison made between the BHV-2 TK gene nucleotide sequence and that of the HS ...
Species abundance distributions and richness estimations in fungal
Species abundance distributions and richness estimations in fungal

... data in microbiology by treating individual DNA sequences as counts of molecular taxonomic units (MOTUs). This study addresses the suitability of species abundance models in three groups of plant-associated fungal communities – phyllosphere, ectomycorrhizal and arbuscular mycorrhizal fungi. We teste ...
Dangerous Ideas and Forbidden Knowledge, Spring 2005 Lab 2
Dangerous Ideas and Forbidden Knowledge, Spring 2005 Lab 2

... allowing scientists to easily amplify short specific regions of DNA for a variety of purposes including gene mapping, cloning, DNA sequencing and gene detection. The objective of PCR is to produce a large amount of DNA in a test tube starting from only a trace amount. A researcher can take trace amo ...
$doc.title

... Problems ...
1. Introduction 2. Analytical methods of identifying source species of
1. Introduction 2. Analytical methods of identifying source species of

... Immunological methods (ELISA, etc.) that use proteins specific to certain species as markers, and physicochemical methods (high performance liquid chromatography, etc.) that exploit the differences in the protein and other components present in different meats have been in use for identification of ...
Can Nurture Influence Nature? - Prof. Sir David Baulcombe
Can Nurture Influence Nature? - Prof. Sir David Baulcombe

... • evolution requires variation in heritable traits • heritable variation can be achieved other than by genetic mutation – epimutation • epimutations differ from genetic mutations in that they may be unstable and in that they can be induced and targeted • RNA can initiate variation that is inherited ...
Zebrafish as a model organism for the study of functional genomics
Zebrafish as a model organism for the study of functional genomics

... In order to investigate the functional roles of novel genes in vertebrates, the generation of transgenic or gene knock-out mice has been wildly established. However, it is an expensive and time-consuming process that cannot be applicable to other vertebrate species. Zebrafish is a good model organis ...
Identification of candidate genes for resource-use
Identification of candidate genes for resource-use

... In this project we aimed to develop a methodology, known as genome wide association mapping, for the identification of plant genes that influence these traits. This is a relatively new and powerful approach that has been made possible by the ever-decreasing cost of genotyping. Genes that are identif ...
DNA research
DNA research

... was the only known B. subtilis gene mapped and characterized in this region.7 The putative products of yojV, yojW, yojX, yojYand yojZshowed no significant homology to any known proteins in the data banks. General features of the five putative gene products that showed homology to other known protein ...
Entity Recognition: Current Status and Summer Plan
Entity Recognition: Current Status and Summer Plan

... • Conjunctive words/phrases tagged differently – E.g.: …three cbl genes (c-cbl , cblb , and cblc) which… – Heuristic: use some rules to capture such conjunctive words, and tag them consistently ...
Full-Text PDF
Full-Text PDF

... Due to the PCR-inhibition of coextracted molecules, the qPCR of this study became positive after 34 to 40 cycles. Even using modern methods such as 16S rRNA-based analysis in next generation sequencing, a PCR has to be performed. As negative control, a sample of water was treated and analyzed in the ...
Gene ontology analysis of gene-gene interactions in two genome
Gene ontology analysis of gene-gene interactions in two genome

... Amyotrophic Lateral Sclerosis (ALS) is a neurological disease that causes motor neuron degeneration, leading to paralysis and eventually death. Around 5,600 people are diagnosed with ALS each year with the incidence rate of two per 100,000 a year [1]. Despite the relatively low incidence rate compar ...
complex polypeptide-1 gene and related sequences
complex polypeptide-1 gene and related sequences

... mRNAs that are differentially expressed in the cell types comprising the seminiferous epithelium of the testis. We described a set of cDNA clones that derives from poly(A) + RNAs that are accumulated in meiotic and postmeiotic cells (Dudley, Potter, Lyon & Willison, 1984). Two lines of evidence show ...
Characteristic Features of the Nucleotide Sequences of Yeast
Characteristic Features of the Nucleotide Sequences of Yeast

... of three nucleotides and their statistically biased appearance. An additional reason is due to the specific usage of synonymous codons depending on the nature of genes and their products as discussed by Ikemura.5 Furthermore, structural constraints within various regions of proteins encoded by respe ...
Chapter 21: Molecular Basis of Cancer
Chapter 21: Molecular Basis of Cancer

... DHPLC analysis at different temperatures of the column ...
Isolation and identification of cobalt‐and caesium‐resistant bacteria
Isolation and identification of cobalt‐and caesium‐resistant bacteria

... DNA sequencing was performed by Source Bioscience using an ABI 3730xl 96 capillary Genome Analyser analysis system. The template of each isolate was provided as a purified PCR product at a concentration of 15 ng lL 1 ...
Supplemental Text. Informational genes undergo inter
Supplemental Text. Informational genes undergo inter

... Acanthamoeba, Paramecium and dictyostelids, but also in some fungi, platyhelminths and molluscs, the vast majority of which have been described to experience low-oxygen conditions (9,10); (ii) many of these homologues are predicted to be targeted to mitochondrion-related organelles (not shown), inc ...
Tutorial_9_NEW
Tutorial_9_NEW

... -other efficient algorithms for identifying stem loops Concentrating on intragenic regions and introns - Filtering coding regions Filtering out non conserved candidates -Mature and pre-miRNA is usually evolutionary conserved ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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