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Mining medical data using multiple corpora
Mining medical data using multiple corpora

... transformation of pleural cells. The first idea was to access automatically GenBank entries corresponding to genes from Transcriptomics server thanks to AccNum. GenBank is part of the International Nucleotide Sequence Database Collaboration, which comprises the DNA DataBank of Japan (DDBJ), the Euro ...
The genus Listonella MacDonell and Colwell 1986 is a later
The genus Listonella MacDonell and Colwell 1986 is a later

... and species of the same genus will form monophyletic groups on the basis of MLSA. The different genera of the Vibrionaceae have less than 70 % AAI. These thresholds were proposed based on a limited number of genome sequences, and we highlighted that the analysis of newly sequenced genomes would refi ...
Discriminate the Falsely Predicted Protein–Coding Genes in
Discriminate the Falsely Predicted Protein–Coding Genes in

... lies in the former has regularly specific features such as asymmetric nucleotide distributions ...
β-lactamase gene blaSHV detected in bacteria
β-lactamase gene blaSHV detected in bacteria

... indicates that there could be other resistance genes other than blaTEM and blaSHV present which may be responsible for encoding the resistance, such as CTX-M. This resistance appears to have arisen through the initial transfer of the chromosomal β-lactamase gene from Kluyvera spp. to conjugative pla ...
Presence of a mitochondrial-type 70
Presence of a mitochondrial-type 70

... fungi (44). The ciliates that possess hydrogenosomes with double bounding membranes are polyphyletic, as evidenced from small subunit ribosomal rRNA-based phylogeny (45). This supports the idea that these hydrogenosomes could derive from mitochondria. Nevertheless, in the fungus Neocallimastix, hydr ...
Prokaryotes
Prokaryotes

... not on any molecular evidence. Using molecular biology (small subunit ribosomal RNA), Carl Woese suggested that some prokaryotes are more closely related to eukaryotes. He suggested that even though they are all made up of prokaryotic cells, one group of bacteria replicated their DNA and made protei ...
Tetrapods
Tetrapods

... not on any molecular evidence. Using molecular biology (small subunit ribosomal RNA), Carl Woese suggested that some prokaryotes are more closely related to eukaryotes. He suggested that even though they are all made up of prokaryotic cells, one group of bacteria replicated their DNA and made protei ...
Experimental General. All the DNA manipulations and bacterial
Experimental General. All the DNA manipulations and bacterial

... Together with the above mutagenic primers, in the first PCRs, BC-LIP-9F (5’CCGCCACGTACAACCAGAACTATC-3’) and PET-2R (5’-GTTATTGCTCAGCGGTGG3’) were also used, and in the second PCR, BC-LIP-9F and PET-2R were used. The conditions for the 100 µL PCR mixture were as follows: 0.5 µM each primer, 0.2 mM ea ...
UBE3B developmental disorders and increased mortality in cattle
UBE3B developmental disorders and increased mortality in cattle

... of the four variants (rs440561578, rs467377722) segregated also among 191 non-Fleckvieh animals that were sequenced in the context of the 1000 bull genomes project [15] and can be therefore excluded as being causative. In conclusion, only a coding and an intronic variant of the UBE3B gene segregate ...
Index case of acute myeloid leukemia in a family
Index case of acute myeloid leukemia in a family

... Invitrogen). Dissociated cells were centrifuged at 1000 rpm, transferred into a tissue-culture flask with complete Amniomax medium to allow cell attachment, and kept at 37°C in a CO2 incubator. Genomic DNA was extracted from cultured fibroblasts using the QIAamp system (Qiagen Inc). The region aroun ...
Simulation of Gene Splicing (Genetic Engineering
Simulation of Gene Splicing (Genetic Engineering

... hormone. In the l950's, it was found that hormone from the pituitaries of dead people could be used as a treatment. However, not enough people donated their glands to supply hormone for all those who needed it. Even more sadly, some of the pituitaries used for this purpose contained a deadly virus. ...
Knox. The Gene Genie.
Knox. The Gene Genie.

... of developing a new class of drugs based on CRISPR. (The company is not yet talking about which diseases it will target first.) In April, CRISPR Therapeutics launched in Basel and London, with investments of $25 million and a similar goal. Therapies from companies like CRISPR Therapeutics and Editas ...
Cloning
Cloning

... or previously existing human. The term is generally used to refer to artificial human cloning; human clones in the form of identical twins are commonplace, with their cloning occurring during the natural process of reproduction. There are two commonly discussed types of human cloning: therapeutic cl ...
Inferring Ancestral Chloroplast Genomes with Inverted
Inferring Ancestral Chloroplast Genomes with Inverted

... clusters may be more likely to be maintained if they share related function and are under constraints in the face of genome rearrangements. 5.2. Simulations to Assess Accuracy Phylogenetic analysis methods deal with lost historic information, thus their accuracy should also be assessed through simul ...
Federal Agency for Social Development
Federal Agency for Social Development

... products that are essential for them to survive in a particular environment. e.g. Bacteria do not synthesize the enzymes needed to make tryptophan when there is an abundant supply of tryptophan in the environment. However, when tryptophan is absent from the environment the enzymes are made. Similarl ...
How Is Genetic Research On Behavior Conducted?
How Is Genetic Research On Behavior Conducted?

... overlap. As just noted, an adoption study might look at pairs of twins that had been adopted away into different families. Some studies have unusual permutations, for example, a family study might include stepchildren who are related to one but not both parents. Furthermore, data from all three kind ...
1. Telomeres 2. Centromeric Repeats 3. Retrotransposons (Class I
1. Telomeres 2. Centromeric Repeats 3. Retrotransposons (Class I

... Three of the Tos family (Tos10, Tos17, Tos19) have been shown to be active under tissue culture conditions Tos17 was found to only have two copies in the Nipponbare genome Tos17, when activated, has a preference for insertion into low copy sequences in the rice genome Tos17 activation leads to a gra ...
Reading frame
Reading frame

... In practice it is treated as a synonym for "computational molecular biology“ ----the use of computers to characterize the molecular components of living things. ...
SNaPshot® Multiplex System for SNP genotyping
SNaPshot® Multiplex System for SNP genotyping

... changes in treated and untreated samples to determine the methylation status. BAC fingerprinting Fingerprinting of large insert genomic fragment libraries, also known as BAC (bacterial artificial chromosomes) clones leads to the construction of a genome-wide physical map. These maps are critical to ...
Practice test 2
Practice test 2

... 7. In 1974, Stanley Cohen and Herbert Boyer inserted a gene from an African clawed frog into a bacterium. The bacterium produced the protein coded for by the inserted frog gene. The bacterium containing functional frog DNA would be classified as a _____. a. clone c. plasmid b. DNA fingerprint d. tra ...
Escherichia coli - Biology - University of Central Oklahoma
Escherichia coli - Biology - University of Central Oklahoma

... Cultures: Five E. coli strains were utilized in this experiment: BW25113, JW1271, JW1272, JC3272F, and JC3272I. They were stored in a -80˚ freezer and streaked on LB agar plates (Figure 1). DNA Extraction: Using an inoculating loop, randomly selected colonies were placed into microcentrifuge tubes c ...
Reference genome sequence of the model plant Setaria
Reference genome sequence of the model plant Setaria

... cvalues/)). The completed assembly was compared against 42 ran­ domly selected, fully sequenced BAC clones derived from the S. italica BAC library and against nine randomly selected, fully sequenced fosmid clones containing DNA from S. viridis accession A10. Of the 51 regions investigated, all were ...
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma

... Genomics and the Identification of APOA5 Recent accomplishments by the Human Genome Project have facilitated genome-wide strategies to uncover functional regions of the mammalian genome. With the increasing availability of genomic sequences from multiple species, comparative genomic approaches have ...
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma
Apolipoprotein A5, a Newly Identified Gene That Affects Plasma

... Genomics and the Identification of APOA5 Recent accomplishments by the Human Genome Project have facilitated genome-wide strategies to uncover functional regions of the mammalian genome. With the increasing availability of genomic sequences from multiple species, comparative genomic approaches have ...
Molecular Evidence for Vector Implication of Onchocerca lupi in Los
Molecular Evidence for Vector Implication of Onchocerca lupi in Los

... The nodules are primarily composed of eosinophils, histiocytes, fibroblasts, and newly formed blood vessels (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). This is typically associated with exophthalmos and possible third-eyelid protrusion (Komnenou, Egyed, Sréter, & Eberhard, 2003). In almost all ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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